A Critical Review on Cryopreservation of Cells Technics: Thermal Aspects
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , |
Tipo de documento: | Artigo de conferência |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/978-3-030-93456-9_16 http://hdl.handle.net/11449/239912 |
Resumo: | The studies carried out in the area of cell preservation have been of significant importance in recent years. This process, known as cryopreservation, has been used for decades and aims to preserve various cellular materials at cryogenic temperatures. Different methods allow cryopreservation, where the best known are the slow freezing and vitrification processes. Regardless of the process, it is necessary to use cryoprotective agents (CPAs) in conjunction with the cells to avoid possible cell damage during cryopreservation. Regarding the methods, they differ in their cooling rates and the concentration of CPAs used. According to some studies, the method that has been most studied is that of vitrification, which seeks to increase the cooling rate, reaching values above 20,000 ∘ C/min. Evaporation by a thin film of liquid nitrogen is one of the methods of vitrification, which uses a microstructure with a finned or porous surface for evaporation, and in these conditions cooling rates of up to 150,000 ∘ C/min were found, which shows a significant advance and alternative for cell cryopreservation. |
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A Critical Review on Cryopreservation of Cells Technics: Thermal AspectsCryopreservationSlow freezingThin film evaporationVitrificationThe studies carried out in the area of cell preservation have been of significant importance in recent years. This process, known as cryopreservation, has been used for decades and aims to preserve various cellular materials at cryogenic temperatures. Different methods allow cryopreservation, where the best known are the slow freezing and vitrification processes. Regardless of the process, it is necessary to use cryoprotective agents (CPAs) in conjunction with the cells to avoid possible cell damage during cryopreservation. Regarding the methods, they differ in their cooling rates and the concentration of CPAs used. According to some studies, the method that has been most studied is that of vitrification, which seeks to increase the cooling rate, reaching values above 20,000 ∘ C/min. Evaporation by a thin film of liquid nitrogen is one of the methods of vitrification, which uses a microstructure with a finned or porous surface for evaporation, and in these conditions cooling rates of up to 150,000 ∘ C/min were found, which shows a significant advance and alternative for cell cryopreservation.the University of Vale do Rio dos Sinos Mechanical Engineering Graduate Program LETEF Laboratory of Thermal and Fluid Dynamic Studies, Unisinos Avenue, 950, RSFSG—University Center Center of Innovation and Technology Department of Mechanical Engineering, Os Dezoito do Forte, 2366, RSUNESP—São Paulo State University Post-Graduation Program in Mechanical Engineering, Brasil Avenue, 56, SPUNESP—São Paulo State University Post-Graduation Program in Mechanical Engineering, Brasil Avenue, 56, SPLaboratory of Thermal and Fluid Dynamic StudiesCenter of Innovation and TechnologyUniversidade Estadual Paulista (UNESP)Steffenon, Guilherme D.Copetti, Jacqueline B.Oliveira, Jeferson D. deMacagnan, Mario H.Cardoso, Elaine M. [UNESP]2023-03-01T19:52:52Z2023-03-01T19:52:52Z2022-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObject175-183http://dx.doi.org/10.1007/978-3-030-93456-9_16Lecture Notes in Mechanical Engineering, p. 175-183.2195-43642195-4356http://hdl.handle.net/11449/23991210.1007/978-3-030-93456-9_162-s2.0-85128512823Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengLecture Notes in Mechanical Engineeringinfo:eu-repo/semantics/openAccess2023-03-01T19:52:52Zoai:repositorio.unesp.br:11449/239912Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:06:21.257187Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
A Critical Review on Cryopreservation of Cells Technics: Thermal Aspects |
title |
A Critical Review on Cryopreservation of Cells Technics: Thermal Aspects |
spellingShingle |
A Critical Review on Cryopreservation of Cells Technics: Thermal Aspects Steffenon, Guilherme D. Cryopreservation Slow freezing Thin film evaporation Vitrification |
title_short |
A Critical Review on Cryopreservation of Cells Technics: Thermal Aspects |
title_full |
A Critical Review on Cryopreservation of Cells Technics: Thermal Aspects |
title_fullStr |
A Critical Review on Cryopreservation of Cells Technics: Thermal Aspects |
title_full_unstemmed |
A Critical Review on Cryopreservation of Cells Technics: Thermal Aspects |
title_sort |
A Critical Review on Cryopreservation of Cells Technics: Thermal Aspects |
author |
Steffenon, Guilherme D. |
author_facet |
Steffenon, Guilherme D. Copetti, Jacqueline B. Oliveira, Jeferson D. de Macagnan, Mario H. Cardoso, Elaine M. [UNESP] |
author_role |
author |
author2 |
Copetti, Jacqueline B. Oliveira, Jeferson D. de Macagnan, Mario H. Cardoso, Elaine M. [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Laboratory of Thermal and Fluid Dynamic Studies Center of Innovation and Technology Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Steffenon, Guilherme D. Copetti, Jacqueline B. Oliveira, Jeferson D. de Macagnan, Mario H. Cardoso, Elaine M. [UNESP] |
dc.subject.por.fl_str_mv |
Cryopreservation Slow freezing Thin film evaporation Vitrification |
topic |
Cryopreservation Slow freezing Thin film evaporation Vitrification |
description |
The studies carried out in the area of cell preservation have been of significant importance in recent years. This process, known as cryopreservation, has been used for decades and aims to preserve various cellular materials at cryogenic temperatures. Different methods allow cryopreservation, where the best known are the slow freezing and vitrification processes. Regardless of the process, it is necessary to use cryoprotective agents (CPAs) in conjunction with the cells to avoid possible cell damage during cryopreservation. Regarding the methods, they differ in their cooling rates and the concentration of CPAs used. According to some studies, the method that has been most studied is that of vitrification, which seeks to increase the cooling rate, reaching values above 20,000 ∘ C/min. Evaporation by a thin film of liquid nitrogen is one of the methods of vitrification, which uses a microstructure with a finned or porous surface for evaporation, and in these conditions cooling rates of up to 150,000 ∘ C/min were found, which shows a significant advance and alternative for cell cryopreservation. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-01-01 2023-03-01T19:52:52Z 2023-03-01T19:52:52Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/conferenceObject |
format |
conferenceObject |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/978-3-030-93456-9_16 Lecture Notes in Mechanical Engineering, p. 175-183. 2195-4364 2195-4356 http://hdl.handle.net/11449/239912 10.1007/978-3-030-93456-9_16 2-s2.0-85128512823 |
url |
http://dx.doi.org/10.1007/978-3-030-93456-9_16 http://hdl.handle.net/11449/239912 |
identifier_str_mv |
Lecture Notes in Mechanical Engineering, p. 175-183. 2195-4364 2195-4356 10.1007/978-3-030-93456-9_16 2-s2.0-85128512823 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Lecture Notes in Mechanical Engineering |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
175-183 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808128895527944192 |