Spermathecae: Morphofunctional features and correlation with fat bodies and trachea in six species of vectors of Chagas disease

Detalhes bibliográficos
Autor(a) principal: Damieli Nascimento, Juliana
Data de Publicação: 2019
Outros Autores: Caneguim, Breno Henrique, de Paula, Mariana Campos, Rimoldi Ribeiro, Aline, Sasso-Cerri, Estela [UNESP], da Rosa, João Aristeu [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.actatropica.2019.05.023
http://hdl.handle.net/11449/187708
Resumo: Since spermatheca is able to transport spermatozoa and maintain a specific microenvironment for the storage of viable sperm cells for long periods of time, specific morphofunctional features must be involved in this capacity, and an efficient nutritional and oxygen supply must be required. In this study, we investigated the histological features of spermathecae and fat bodies in six species of three genera of epidemiological importance for Chagas’ disease. The association of the reproductive system with the fat bodies and tracheal system was also focused in these species. The reproductive system, tracheae and fat bodies were fixed in 4% formaldehyde, and embedded in glycol methacrylate. The sections were stained with H.E., picrosirius red and Periodic-Acid Schiff methods for morphological analyses. Paraffin-embedded spermatheca sections were submitted to immunofluorescence for detection of V-ATPase. In P. lignarius, R. montenegrensis and R. prolixus, the spermatheca contains a slightly dilated tubular distal portion. In P. megistus and T. tibiamaculata, the spermatheca shows a large bulbous distal portion, and in T. infestans, a large oval-shaped distal portion. In all species, this portion was surrounded by a thin muscular layer, and the epithelial height varied according to the shape of this terminal portion. All spermathecal proximal portions showed simple columnar epithelium surrounded by a thick muscular layer. The epithelial cells of spermathecae showed PAS-positive cytoplasm and V-ATPase immunofluorescence in the apical surface. Tracheoles and polysaccharide-rich fat body cells were found next or in close contact to the oviduct or spermathecal tissues. The results indicate that the spermatheca proximal portion is related to contraction and sperm transport, whose oxygen and energy supply is guaranteed by the associated tracheal branches and fat bodies. In the storage portion, fat bodies and tracheae seem to be crucial for the maintenance of an optimal spermathecal microenvironment and storage of viable sperm cells. The participation of V-ATPase in the spermathecae epithelial cells may contribute for the maintenance of an optimal luminal milieu to spermatozoa, by alkalinization and/or acidification of lumen, similarly to the other epithelial cell types in insects. Further studies are necessary to clarify the role of this proton pump in the spermathecal epithelial cells.
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spelling Spermathecae: Morphofunctional features and correlation with fat bodies and trachea in six species of vectors of Chagas diseaseFat bodyMorphologySpermathecaTracheaTriatominaeTrophocytesV-ATPaseSince spermatheca is able to transport spermatozoa and maintain a specific microenvironment for the storage of viable sperm cells for long periods of time, specific morphofunctional features must be involved in this capacity, and an efficient nutritional and oxygen supply must be required. In this study, we investigated the histological features of spermathecae and fat bodies in six species of three genera of epidemiological importance for Chagas’ disease. The association of the reproductive system with the fat bodies and tracheal system was also focused in these species. The reproductive system, tracheae and fat bodies were fixed in 4% formaldehyde, and embedded in glycol methacrylate. The sections were stained with H.E., picrosirius red and Periodic-Acid Schiff methods for morphological analyses. Paraffin-embedded spermatheca sections were submitted to immunofluorescence for detection of V-ATPase. In P. lignarius, R. montenegrensis and R. prolixus, the spermatheca contains a slightly dilated tubular distal portion. In P. megistus and T. tibiamaculata, the spermatheca shows a large bulbous distal portion, and in T. infestans, a large oval-shaped distal portion. In all species, this portion was surrounded by a thin muscular layer, and the epithelial height varied according to the shape of this terminal portion. All spermathecal proximal portions showed simple columnar epithelium surrounded by a thick muscular layer. The epithelial cells of spermathecae showed PAS-positive cytoplasm and V-ATPase immunofluorescence in the apical surface. Tracheoles and polysaccharide-rich fat body cells were found next or in close contact to the oviduct or spermathecal tissues. The results indicate that the spermatheca proximal portion is related to contraction and sperm transport, whose oxygen and energy supply is guaranteed by the associated tracheal branches and fat bodies. In the storage portion, fat bodies and tracheae seem to be crucial for the maintenance of an optimal spermathecal microenvironment and storage of viable sperm cells. The participation of V-ATPase in the spermathecae epithelial cells may contribute for the maintenance of an optimal luminal milieu to spermatozoa, by alkalinization and/or acidification of lumen, similarly to the other epithelial cell types in insects. Further studies are necessary to clarify the role of this proton pump in the spermathecal epithelial cells.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Biology Institute Department of Animal Biology – Parasitology University of Campinas – UNICAMPDepartment of Morphology and Genetics Federal University of São PauloUniversity of AraraquaraDepartment of Morphology Dental School of São Paulo State University (UNESP)São Paulo State University (UNESP) School of Pharmaceutical SciencesDepartment of Morphology Dental School of São Paulo State University (UNESP)São Paulo State University (UNESP) School of Pharmaceutical SciencesUniversidade Estadual de Campinas (UNICAMP)Universidade de São Paulo (USP)University of AraraquaraUniversidade Estadual Paulista (Unesp)Damieli Nascimento, JulianaCaneguim, Breno Henriquede Paula, Mariana CamposRimoldi Ribeiro, AlineSasso-Cerri, Estela [UNESP]da Rosa, João Aristeu [UNESP]2019-10-06T15:44:48Z2019-10-06T15:44:48Z2019-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.actatropica.2019.05.023Acta Tropica, v. 197.1873-62540001-706Xhttp://hdl.handle.net/11449/18770810.1016/j.actatropica.2019.05.0232-s2.0-85066492174Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengActa Tropicainfo:eu-repo/semantics/openAccess2021-10-23T20:19:25Zoai:repositorio.unesp.br:11449/187708Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:33:00.107256Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Spermathecae: Morphofunctional features and correlation with fat bodies and trachea in six species of vectors of Chagas disease
title Spermathecae: Morphofunctional features and correlation with fat bodies and trachea in six species of vectors of Chagas disease
spellingShingle Spermathecae: Morphofunctional features and correlation with fat bodies and trachea in six species of vectors of Chagas disease
Damieli Nascimento, Juliana
Fat body
Morphology
Spermatheca
Trachea
Triatominae
Trophocytes
V-ATPase
title_short Spermathecae: Morphofunctional features and correlation with fat bodies and trachea in six species of vectors of Chagas disease
title_full Spermathecae: Morphofunctional features and correlation with fat bodies and trachea in six species of vectors of Chagas disease
title_fullStr Spermathecae: Morphofunctional features and correlation with fat bodies and trachea in six species of vectors of Chagas disease
title_full_unstemmed Spermathecae: Morphofunctional features and correlation with fat bodies and trachea in six species of vectors of Chagas disease
title_sort Spermathecae: Morphofunctional features and correlation with fat bodies and trachea in six species of vectors of Chagas disease
author Damieli Nascimento, Juliana
author_facet Damieli Nascimento, Juliana
Caneguim, Breno Henrique
de Paula, Mariana Campos
Rimoldi Ribeiro, Aline
Sasso-Cerri, Estela [UNESP]
da Rosa, João Aristeu [UNESP]
author_role author
author2 Caneguim, Breno Henrique
de Paula, Mariana Campos
Rimoldi Ribeiro, Aline
Sasso-Cerri, Estela [UNESP]
da Rosa, João Aristeu [UNESP]
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual de Campinas (UNICAMP)
Universidade de São Paulo (USP)
University of Araraquara
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Damieli Nascimento, Juliana
Caneguim, Breno Henrique
de Paula, Mariana Campos
Rimoldi Ribeiro, Aline
Sasso-Cerri, Estela [UNESP]
da Rosa, João Aristeu [UNESP]
dc.subject.por.fl_str_mv Fat body
Morphology
Spermatheca
Trachea
Triatominae
Trophocytes
V-ATPase
topic Fat body
Morphology
Spermatheca
Trachea
Triatominae
Trophocytes
V-ATPase
description Since spermatheca is able to transport spermatozoa and maintain a specific microenvironment for the storage of viable sperm cells for long periods of time, specific morphofunctional features must be involved in this capacity, and an efficient nutritional and oxygen supply must be required. In this study, we investigated the histological features of spermathecae and fat bodies in six species of three genera of epidemiological importance for Chagas’ disease. The association of the reproductive system with the fat bodies and tracheal system was also focused in these species. The reproductive system, tracheae and fat bodies were fixed in 4% formaldehyde, and embedded in glycol methacrylate. The sections were stained with H.E., picrosirius red and Periodic-Acid Schiff methods for morphological analyses. Paraffin-embedded spermatheca sections were submitted to immunofluorescence for detection of V-ATPase. In P. lignarius, R. montenegrensis and R. prolixus, the spermatheca contains a slightly dilated tubular distal portion. In P. megistus and T. tibiamaculata, the spermatheca shows a large bulbous distal portion, and in T. infestans, a large oval-shaped distal portion. In all species, this portion was surrounded by a thin muscular layer, and the epithelial height varied according to the shape of this terminal portion. All spermathecal proximal portions showed simple columnar epithelium surrounded by a thick muscular layer. The epithelial cells of spermathecae showed PAS-positive cytoplasm and V-ATPase immunofluorescence in the apical surface. Tracheoles and polysaccharide-rich fat body cells were found next or in close contact to the oviduct or spermathecal tissues. The results indicate that the spermatheca proximal portion is related to contraction and sperm transport, whose oxygen and energy supply is guaranteed by the associated tracheal branches and fat bodies. In the storage portion, fat bodies and tracheae seem to be crucial for the maintenance of an optimal spermathecal microenvironment and storage of viable sperm cells. The participation of V-ATPase in the spermathecae epithelial cells may contribute for the maintenance of an optimal luminal milieu to spermatozoa, by alkalinization and/or acidification of lumen, similarly to the other epithelial cell types in insects. Further studies are necessary to clarify the role of this proton pump in the spermathecal epithelial cells.
publishDate 2019
dc.date.none.fl_str_mv 2019-10-06T15:44:48Z
2019-10-06T15:44:48Z
2019-09-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.actatropica.2019.05.023
Acta Tropica, v. 197.
1873-6254
0001-706X
http://hdl.handle.net/11449/187708
10.1016/j.actatropica.2019.05.023
2-s2.0-85066492174
url http://dx.doi.org/10.1016/j.actatropica.2019.05.023
http://hdl.handle.net/11449/187708
identifier_str_mv Acta Tropica, v. 197.
1873-6254
0001-706X
10.1016/j.actatropica.2019.05.023
2-s2.0-85066492174
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Acta Tropica
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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