Alginate-based microparticles coated with HPMCP/AS cellulose-derivatives enable the Ctx(Ile21)-Ha antimicrobial peptide application as a feed additive

Detalhes bibliográficos
Autor(a) principal: Roque-Borda, Cesar Augusto [UNESP]
Data de Publicação: 2021
Outros Autores: Silva, Hanyeny Raiely Leite [UNESP], Crusca Junior, Edson [UNESP], Serafim, Jéssica Aparecida [UNESP], Meneguin, Andréia Bagliotti [UNESP], Chorilli, Marlus [UNESP], Macedo, Wagner Costa [UNESP], Teixeira, Silvio Rainho [UNESP], Guastalli, Elisabete Aparecida Lopes, Soares, Nilce Maria, Blair, Jessica M.A., Pikramenou, Zoe, Vicente, Eduardo Festozo [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.ijbiomac.2021.05.011
http://hdl.handle.net/11449/207738
Resumo: Microencapsulation is a potential biotechnological tool, which can overcome antimicrobial peptides (AMP) instabilities and reduce toxic side effects. Thus, this study evaluates the antibacterial activities of the Ctx(Ile21)-Ha AMP against multidrug-resistant (MDR) and non-resistant bacteria and develop and characterize peptide-loaded microparticles coated with the enteric polymers hydroxypropylmethylcellulose acetate succinate (HPMCAS) and hydroxypropylmethylcellulose phthalate (HPMCP). Ctx(Ile21)-Ha was obtained by solid phase peptide synthesis (SPPS) method, purified and characterized by HPLC and Mass Spectrometry. The peptide exhibited potent antibiotic activities against Salmonella enteritidis, Salmonella typhimurium, Pseudomonas aeruginosa (MDR), Acinetobacter baumannii (MDR), and Staphylococcus aureus (MDR). Ctx(Ile21)-Ha microencapsulation was performed by ionic gelation with high efficiency, maintaining the physical-chemical stability. Ctx(Ile21)-Ha coated-microparticles were characterized by DSC, TGA, FTIR-Raman, XRD and SEM. Hemolytic activity assay demonstrated that hemolysis was decreased up to 95% compared to single molecule. In addition, in vitro release control profile simulating different portions of gastrointestinal tract was performed and showed the microcapsules' ability to protect the peptide and release it in the intestine, aiming pathogen's location, mainly by Salmonella sp. Therefore, use of microencapsulated Ctx(Ile21)-Ha can be allowed as an antimicrobial controller in monogastric animal production as an oral feed additive (antimicrobial controller), being a valuable option for molecules with low therapeutic indexes or high hemolytic rates.
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spelling Alginate-based microparticles coated with HPMCP/AS cellulose-derivatives enable the Ctx(Ile21)-Ha antimicrobial peptide application as a feed additiveAMPAntibacterial activityMicrocapsulesMicroencapsulation is a potential biotechnological tool, which can overcome antimicrobial peptides (AMP) instabilities and reduce toxic side effects. Thus, this study evaluates the antibacterial activities of the Ctx(Ile21)-Ha AMP against multidrug-resistant (MDR) and non-resistant bacteria and develop and characterize peptide-loaded microparticles coated with the enteric polymers hydroxypropylmethylcellulose acetate succinate (HPMCAS) and hydroxypropylmethylcellulose phthalate (HPMCP). Ctx(Ile21)-Ha was obtained by solid phase peptide synthesis (SPPS) method, purified and characterized by HPLC and Mass Spectrometry. The peptide exhibited potent antibiotic activities against Salmonella enteritidis, Salmonella typhimurium, Pseudomonas aeruginosa (MDR), Acinetobacter baumannii (MDR), and Staphylococcus aureus (MDR). Ctx(Ile21)-Ha microencapsulation was performed by ionic gelation with high efficiency, maintaining the physical-chemical stability. Ctx(Ile21)-Ha coated-microparticles were characterized by DSC, TGA, FTIR-Raman, XRD and SEM. Hemolytic activity assay demonstrated that hemolysis was decreased up to 95% compared to single molecule. In addition, in vitro release control profile simulating different portions of gastrointestinal tract was performed and showed the microcapsules' ability to protect the peptide and release it in the intestine, aiming pathogen's location, mainly by Salmonella sp. Therefore, use of microencapsulated Ctx(Ile21)-Ha can be allowed as an antimicrobial controller in monogastric animal production as an oral feed additive (antimicrobial controller), being a valuable option for molecules with low therapeutic indexes or high hemolytic rates.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)São Paulo State University (Unesp) School of Agricultural and Veterinarian Sciences, JaboticabalSão Paulo State University (Unesp) Institute of Chemistry, AraraquaraSão Paulo State University (Unesp) School of Sciences and Engineering, TupãSão Paulo State University (Unesp) School of Pharmaceutical Sciences, AraraquaraSão Paulo State University (Unesp) School of Technology and Sciences, Presidente PrudentePoultry Health Specialized Laboratory Biological Institute, BastosInstitute of Microbiology and Infection University of BirminghamSchool of Chemistry University of BirminghamSão Paulo State University (Unesp) School of Agricultural and Veterinarian Sciences, JaboticabalSão Paulo State University (Unesp) Institute of Chemistry, AraraquaraSão Paulo State University (Unesp) School of Sciences and Engineering, TupãSão Paulo State University (Unesp) School of Pharmaceutical Sciences, AraraquaraSão Paulo State University (Unesp) School of Technology and Sciences, Presidente PrudenteFAPESP: 2016/00446-7Universidade Estadual Paulista (Unesp)Biological InstituteUniversity of BirminghamRoque-Borda, Cesar Augusto [UNESP]Silva, Hanyeny Raiely Leite [UNESP]Crusca Junior, Edson [UNESP]Serafim, Jéssica Aparecida [UNESP]Meneguin, Andréia Bagliotti [UNESP]Chorilli, Marlus [UNESP]Macedo, Wagner Costa [UNESP]Teixeira, Silvio Rainho [UNESP]Guastalli, Elisabete Aparecida LopesSoares, Nilce MariaBlair, Jessica M.A.Pikramenou, ZoeVicente, Eduardo Festozo [UNESP]2021-06-25T11:00:11Z2021-06-25T11:00:11Z2021-07-31info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1236-1247http://dx.doi.org/10.1016/j.ijbiomac.2021.05.011International Journal of Biological Macromolecules, v. 183, p. 1236-1247.1879-00030141-8130http://hdl.handle.net/11449/20773810.1016/j.ijbiomac.2021.05.0112-s2.0-85105883464Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInternational Journal of Biological Macromoleculesinfo:eu-repo/semantics/openAccess2024-06-18T18:17:53Zoai:repositorio.unesp.br:11449/207738Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:30:30.713752Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Alginate-based microparticles coated with HPMCP/AS cellulose-derivatives enable the Ctx(Ile21)-Ha antimicrobial peptide application as a feed additive
title Alginate-based microparticles coated with HPMCP/AS cellulose-derivatives enable the Ctx(Ile21)-Ha antimicrobial peptide application as a feed additive
spellingShingle Alginate-based microparticles coated with HPMCP/AS cellulose-derivatives enable the Ctx(Ile21)-Ha antimicrobial peptide application as a feed additive
Roque-Borda, Cesar Augusto [UNESP]
AMP
Antibacterial activity
Microcapsules
title_short Alginate-based microparticles coated with HPMCP/AS cellulose-derivatives enable the Ctx(Ile21)-Ha antimicrobial peptide application as a feed additive
title_full Alginate-based microparticles coated with HPMCP/AS cellulose-derivatives enable the Ctx(Ile21)-Ha antimicrobial peptide application as a feed additive
title_fullStr Alginate-based microparticles coated with HPMCP/AS cellulose-derivatives enable the Ctx(Ile21)-Ha antimicrobial peptide application as a feed additive
title_full_unstemmed Alginate-based microparticles coated with HPMCP/AS cellulose-derivatives enable the Ctx(Ile21)-Ha antimicrobial peptide application as a feed additive
title_sort Alginate-based microparticles coated with HPMCP/AS cellulose-derivatives enable the Ctx(Ile21)-Ha antimicrobial peptide application as a feed additive
author Roque-Borda, Cesar Augusto [UNESP]
author_facet Roque-Borda, Cesar Augusto [UNESP]
Silva, Hanyeny Raiely Leite [UNESP]
Crusca Junior, Edson [UNESP]
Serafim, Jéssica Aparecida [UNESP]
Meneguin, Andréia Bagliotti [UNESP]
Chorilli, Marlus [UNESP]
Macedo, Wagner Costa [UNESP]
Teixeira, Silvio Rainho [UNESP]
Guastalli, Elisabete Aparecida Lopes
Soares, Nilce Maria
Blair, Jessica M.A.
Pikramenou, Zoe
Vicente, Eduardo Festozo [UNESP]
author_role author
author2 Silva, Hanyeny Raiely Leite [UNESP]
Crusca Junior, Edson [UNESP]
Serafim, Jéssica Aparecida [UNESP]
Meneguin, Andréia Bagliotti [UNESP]
Chorilli, Marlus [UNESP]
Macedo, Wagner Costa [UNESP]
Teixeira, Silvio Rainho [UNESP]
Guastalli, Elisabete Aparecida Lopes
Soares, Nilce Maria
Blair, Jessica M.A.
Pikramenou, Zoe
Vicente, Eduardo Festozo [UNESP]
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Biological Institute
University of Birmingham
dc.contributor.author.fl_str_mv Roque-Borda, Cesar Augusto [UNESP]
Silva, Hanyeny Raiely Leite [UNESP]
Crusca Junior, Edson [UNESP]
Serafim, Jéssica Aparecida [UNESP]
Meneguin, Andréia Bagliotti [UNESP]
Chorilli, Marlus [UNESP]
Macedo, Wagner Costa [UNESP]
Teixeira, Silvio Rainho [UNESP]
Guastalli, Elisabete Aparecida Lopes
Soares, Nilce Maria
Blair, Jessica M.A.
Pikramenou, Zoe
Vicente, Eduardo Festozo [UNESP]
dc.subject.por.fl_str_mv AMP
Antibacterial activity
Microcapsules
topic AMP
Antibacterial activity
Microcapsules
description Microencapsulation is a potential biotechnological tool, which can overcome antimicrobial peptides (AMP) instabilities and reduce toxic side effects. Thus, this study evaluates the antibacterial activities of the Ctx(Ile21)-Ha AMP against multidrug-resistant (MDR) and non-resistant bacteria and develop and characterize peptide-loaded microparticles coated with the enteric polymers hydroxypropylmethylcellulose acetate succinate (HPMCAS) and hydroxypropylmethylcellulose phthalate (HPMCP). Ctx(Ile21)-Ha was obtained by solid phase peptide synthesis (SPPS) method, purified and characterized by HPLC and Mass Spectrometry. The peptide exhibited potent antibiotic activities against Salmonella enteritidis, Salmonella typhimurium, Pseudomonas aeruginosa (MDR), Acinetobacter baumannii (MDR), and Staphylococcus aureus (MDR). Ctx(Ile21)-Ha microencapsulation was performed by ionic gelation with high efficiency, maintaining the physical-chemical stability. Ctx(Ile21)-Ha coated-microparticles were characterized by DSC, TGA, FTIR-Raman, XRD and SEM. Hemolytic activity assay demonstrated that hemolysis was decreased up to 95% compared to single molecule. In addition, in vitro release control profile simulating different portions of gastrointestinal tract was performed and showed the microcapsules' ability to protect the peptide and release it in the intestine, aiming pathogen's location, mainly by Salmonella sp. Therefore, use of microencapsulated Ctx(Ile21)-Ha can be allowed as an antimicrobial controller in monogastric animal production as an oral feed additive (antimicrobial controller), being a valuable option for molecules with low therapeutic indexes or high hemolytic rates.
publishDate 2021
dc.date.none.fl_str_mv 2021-06-25T11:00:11Z
2021-06-25T11:00:11Z
2021-07-31
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.ijbiomac.2021.05.011
International Journal of Biological Macromolecules, v. 183, p. 1236-1247.
1879-0003
0141-8130
http://hdl.handle.net/11449/207738
10.1016/j.ijbiomac.2021.05.011
2-s2.0-85105883464
url http://dx.doi.org/10.1016/j.ijbiomac.2021.05.011
http://hdl.handle.net/11449/207738
identifier_str_mv International Journal of Biological Macromolecules, v. 183, p. 1236-1247.
1879-0003
0141-8130
10.1016/j.ijbiomac.2021.05.011
2-s2.0-85105883464
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv International Journal of Biological Macromolecules
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 1236-1247
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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