Alginate-based microparticles coated with HPMCP/AS cellulose-derivatives enable the Ctx(Ile21)-Ha antimicrobial peptide application as a feed additive
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.ijbiomac.2021.05.011 http://hdl.handle.net/11449/207738 |
Resumo: | Microencapsulation is a potential biotechnological tool, which can overcome antimicrobial peptides (AMP) instabilities and reduce toxic side effects. Thus, this study evaluates the antibacterial activities of the Ctx(Ile21)-Ha AMP against multidrug-resistant (MDR) and non-resistant bacteria and develop and characterize peptide-loaded microparticles coated with the enteric polymers hydroxypropylmethylcellulose acetate succinate (HPMCAS) and hydroxypropylmethylcellulose phthalate (HPMCP). Ctx(Ile21)-Ha was obtained by solid phase peptide synthesis (SPPS) method, purified and characterized by HPLC and Mass Spectrometry. The peptide exhibited potent antibiotic activities against Salmonella enteritidis, Salmonella typhimurium, Pseudomonas aeruginosa (MDR), Acinetobacter baumannii (MDR), and Staphylococcus aureus (MDR). Ctx(Ile21)-Ha microencapsulation was performed by ionic gelation with high efficiency, maintaining the physical-chemical stability. Ctx(Ile21)-Ha coated-microparticles were characterized by DSC, TGA, FTIR-Raman, XRD and SEM. Hemolytic activity assay demonstrated that hemolysis was decreased up to 95% compared to single molecule. In addition, in vitro release control profile simulating different portions of gastrointestinal tract was performed and showed the microcapsules' ability to protect the peptide and release it in the intestine, aiming pathogen's location, mainly by Salmonella sp. Therefore, use of microencapsulated Ctx(Ile21)-Ha can be allowed as an antimicrobial controller in monogastric animal production as an oral feed additive (antimicrobial controller), being a valuable option for molecules with low therapeutic indexes or high hemolytic rates. |
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Repositório Institucional da UNESP |
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Alginate-based microparticles coated with HPMCP/AS cellulose-derivatives enable the Ctx(Ile21)-Ha antimicrobial peptide application as a feed additiveAMPAntibacterial activityMicrocapsulesMicroencapsulation is a potential biotechnological tool, which can overcome antimicrobial peptides (AMP) instabilities and reduce toxic side effects. Thus, this study evaluates the antibacterial activities of the Ctx(Ile21)-Ha AMP against multidrug-resistant (MDR) and non-resistant bacteria and develop and characterize peptide-loaded microparticles coated with the enteric polymers hydroxypropylmethylcellulose acetate succinate (HPMCAS) and hydroxypropylmethylcellulose phthalate (HPMCP). Ctx(Ile21)-Ha was obtained by solid phase peptide synthesis (SPPS) method, purified and characterized by HPLC and Mass Spectrometry. The peptide exhibited potent antibiotic activities against Salmonella enteritidis, Salmonella typhimurium, Pseudomonas aeruginosa (MDR), Acinetobacter baumannii (MDR), and Staphylococcus aureus (MDR). Ctx(Ile21)-Ha microencapsulation was performed by ionic gelation with high efficiency, maintaining the physical-chemical stability. Ctx(Ile21)-Ha coated-microparticles were characterized by DSC, TGA, FTIR-Raman, XRD and SEM. Hemolytic activity assay demonstrated that hemolysis was decreased up to 95% compared to single molecule. In addition, in vitro release control profile simulating different portions of gastrointestinal tract was performed and showed the microcapsules' ability to protect the peptide and release it in the intestine, aiming pathogen's location, mainly by Salmonella sp. Therefore, use of microencapsulated Ctx(Ile21)-Ha can be allowed as an antimicrobial controller in monogastric animal production as an oral feed additive (antimicrobial controller), being a valuable option for molecules with low therapeutic indexes or high hemolytic rates.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)São Paulo State University (Unesp) School of Agricultural and Veterinarian Sciences, JaboticabalSão Paulo State University (Unesp) Institute of Chemistry, AraraquaraSão Paulo State University (Unesp) School of Sciences and Engineering, TupãSão Paulo State University (Unesp) School of Pharmaceutical Sciences, AraraquaraSão Paulo State University (Unesp) School of Technology and Sciences, Presidente PrudentePoultry Health Specialized Laboratory Biological Institute, BastosInstitute of Microbiology and Infection University of BirminghamSchool of Chemistry University of BirminghamSão Paulo State University (Unesp) School of Agricultural and Veterinarian Sciences, JaboticabalSão Paulo State University (Unesp) Institute of Chemistry, AraraquaraSão Paulo State University (Unesp) School of Sciences and Engineering, TupãSão Paulo State University (Unesp) School of Pharmaceutical Sciences, AraraquaraSão Paulo State University (Unesp) School of Technology and Sciences, Presidente PrudenteFAPESP: 2016/00446-7Universidade Estadual Paulista (Unesp)Biological InstituteUniversity of BirminghamRoque-Borda, Cesar Augusto [UNESP]Silva, Hanyeny Raiely Leite [UNESP]Crusca Junior, Edson [UNESP]Serafim, Jéssica Aparecida [UNESP]Meneguin, Andréia Bagliotti [UNESP]Chorilli, Marlus [UNESP]Macedo, Wagner Costa [UNESP]Teixeira, Silvio Rainho [UNESP]Guastalli, Elisabete Aparecida LopesSoares, Nilce MariaBlair, Jessica M.A.Pikramenou, ZoeVicente, Eduardo Festozo [UNESP]2021-06-25T11:00:11Z2021-06-25T11:00:11Z2021-07-31info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1236-1247http://dx.doi.org/10.1016/j.ijbiomac.2021.05.011International Journal of Biological Macromolecules, v. 183, p. 1236-1247.1879-00030141-8130http://hdl.handle.net/11449/20773810.1016/j.ijbiomac.2021.05.0112-s2.0-85105883464Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInternational Journal of Biological Macromoleculesinfo:eu-repo/semantics/openAccess2024-06-18T18:17:53Zoai:repositorio.unesp.br:11449/207738Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:30:30.713752Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Alginate-based microparticles coated with HPMCP/AS cellulose-derivatives enable the Ctx(Ile21)-Ha antimicrobial peptide application as a feed additive |
title |
Alginate-based microparticles coated with HPMCP/AS cellulose-derivatives enable the Ctx(Ile21)-Ha antimicrobial peptide application as a feed additive |
spellingShingle |
Alginate-based microparticles coated with HPMCP/AS cellulose-derivatives enable the Ctx(Ile21)-Ha antimicrobial peptide application as a feed additive Roque-Borda, Cesar Augusto [UNESP] AMP Antibacterial activity Microcapsules |
title_short |
Alginate-based microparticles coated with HPMCP/AS cellulose-derivatives enable the Ctx(Ile21)-Ha antimicrobial peptide application as a feed additive |
title_full |
Alginate-based microparticles coated with HPMCP/AS cellulose-derivatives enable the Ctx(Ile21)-Ha antimicrobial peptide application as a feed additive |
title_fullStr |
Alginate-based microparticles coated with HPMCP/AS cellulose-derivatives enable the Ctx(Ile21)-Ha antimicrobial peptide application as a feed additive |
title_full_unstemmed |
Alginate-based microparticles coated with HPMCP/AS cellulose-derivatives enable the Ctx(Ile21)-Ha antimicrobial peptide application as a feed additive |
title_sort |
Alginate-based microparticles coated with HPMCP/AS cellulose-derivatives enable the Ctx(Ile21)-Ha antimicrobial peptide application as a feed additive |
author |
Roque-Borda, Cesar Augusto [UNESP] |
author_facet |
Roque-Borda, Cesar Augusto [UNESP] Silva, Hanyeny Raiely Leite [UNESP] Crusca Junior, Edson [UNESP] Serafim, Jéssica Aparecida [UNESP] Meneguin, Andréia Bagliotti [UNESP] Chorilli, Marlus [UNESP] Macedo, Wagner Costa [UNESP] Teixeira, Silvio Rainho [UNESP] Guastalli, Elisabete Aparecida Lopes Soares, Nilce Maria Blair, Jessica M.A. Pikramenou, Zoe Vicente, Eduardo Festozo [UNESP] |
author_role |
author |
author2 |
Silva, Hanyeny Raiely Leite [UNESP] Crusca Junior, Edson [UNESP] Serafim, Jéssica Aparecida [UNESP] Meneguin, Andréia Bagliotti [UNESP] Chorilli, Marlus [UNESP] Macedo, Wagner Costa [UNESP] Teixeira, Silvio Rainho [UNESP] Guastalli, Elisabete Aparecida Lopes Soares, Nilce Maria Blair, Jessica M.A. Pikramenou, Zoe Vicente, Eduardo Festozo [UNESP] |
author2_role |
author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Biological Institute University of Birmingham |
dc.contributor.author.fl_str_mv |
Roque-Borda, Cesar Augusto [UNESP] Silva, Hanyeny Raiely Leite [UNESP] Crusca Junior, Edson [UNESP] Serafim, Jéssica Aparecida [UNESP] Meneguin, Andréia Bagliotti [UNESP] Chorilli, Marlus [UNESP] Macedo, Wagner Costa [UNESP] Teixeira, Silvio Rainho [UNESP] Guastalli, Elisabete Aparecida Lopes Soares, Nilce Maria Blair, Jessica M.A. Pikramenou, Zoe Vicente, Eduardo Festozo [UNESP] |
dc.subject.por.fl_str_mv |
AMP Antibacterial activity Microcapsules |
topic |
AMP Antibacterial activity Microcapsules |
description |
Microencapsulation is a potential biotechnological tool, which can overcome antimicrobial peptides (AMP) instabilities and reduce toxic side effects. Thus, this study evaluates the antibacterial activities of the Ctx(Ile21)-Ha AMP against multidrug-resistant (MDR) and non-resistant bacteria and develop and characterize peptide-loaded microparticles coated with the enteric polymers hydroxypropylmethylcellulose acetate succinate (HPMCAS) and hydroxypropylmethylcellulose phthalate (HPMCP). Ctx(Ile21)-Ha was obtained by solid phase peptide synthesis (SPPS) method, purified and characterized by HPLC and Mass Spectrometry. The peptide exhibited potent antibiotic activities against Salmonella enteritidis, Salmonella typhimurium, Pseudomonas aeruginosa (MDR), Acinetobacter baumannii (MDR), and Staphylococcus aureus (MDR). Ctx(Ile21)-Ha microencapsulation was performed by ionic gelation with high efficiency, maintaining the physical-chemical stability. Ctx(Ile21)-Ha coated-microparticles were characterized by DSC, TGA, FTIR-Raman, XRD and SEM. Hemolytic activity assay demonstrated that hemolysis was decreased up to 95% compared to single molecule. In addition, in vitro release control profile simulating different portions of gastrointestinal tract was performed and showed the microcapsules' ability to protect the peptide and release it in the intestine, aiming pathogen's location, mainly by Salmonella sp. Therefore, use of microencapsulated Ctx(Ile21)-Ha can be allowed as an antimicrobial controller in monogastric animal production as an oral feed additive (antimicrobial controller), being a valuable option for molecules with low therapeutic indexes or high hemolytic rates. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T11:00:11Z 2021-06-25T11:00:11Z 2021-07-31 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.ijbiomac.2021.05.011 International Journal of Biological Macromolecules, v. 183, p. 1236-1247. 1879-0003 0141-8130 http://hdl.handle.net/11449/207738 10.1016/j.ijbiomac.2021.05.011 2-s2.0-85105883464 |
url |
http://dx.doi.org/10.1016/j.ijbiomac.2021.05.011 http://hdl.handle.net/11449/207738 |
identifier_str_mv |
International Journal of Biological Macromolecules, v. 183, p. 1236-1247. 1879-0003 0141-8130 10.1016/j.ijbiomac.2021.05.011 2-s2.0-85105883464 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
International Journal of Biological Macromolecules |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1236-1247 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128524354060288 |