Biocompatibilidade de cimentos experimentais comparativamente ao Pro-Root MTA: estudo em tecido subcutâneo de ratos

• Autor(a) principal: Rey, Marcela Ito [UNESP]
• Data de Publicação: 2014
• Tipo de documento: Trabalho de conclusão de curso
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/149574; http://www.athena.biblioteca.unesp.br/exlibris/bd/capelo/2017-01-27/000866880.pdf
• topic: Biocompatibilidade; Cimentos dentários; Endodontia; Endodontics

An essential property of endodontic materials is biological compatibility, since not observed, can wake the occurrence of a series of pathological events unfavorable. One of the most practical and used methods for the study of tissue response to endodontic sealers is the implantation of polyethylene tubes containing these materials in the subcutaneous tissue of rats. Given the development of experimental sealers, which contain calcium hydroxide in its formulation, we propose to evaluate the tissue response to these sealers, comparing them to the Mineral Trióxidos Aggregate, Pro-Root® MTA. Were used 56 polyethylene tubes and 14 rats (four tubes per rat) divided into 4 groups: G1 - control (empty tubes), G2 - tubes containing SealAta; G3 - tubes containing SealAta Plus; G4 - tubes containing the ProRoot® MTA. The evaluation time was 7 and 30 days. After each postoperative period, seven animals were euthanized and the polyethylene tubes were removed with the surrounding tissue and fixed in 10% formalin. The specimens were processed for light microscopy analysis, with inclusion in glycol methacrylate, cut serial 3μm and stained with hematoxylin and eosin. The tissue sections were evaluated by pre-established criteria by assigning scores to the microscopic events observed. Data were analyzed from a statistical viewpoint using the Kruskall Wallis and Dunn with a significance level of 5% (p <0.05). It can be observed mild to moderate inflammatory response at 7 days, which was reduced at day 30 for all materials.The experimental sealers showed tissue response similar to sealer Pro-Root® MTA at 7 (p=0.85) and at 30 days (p=0.89).It can be concluded that the experimental sealers SealAta and SealAta Plus are biocompatible, producing similar reactions to Pro-Root® MTA