Eco-Friendly Pharmaceutical Analysis of Rifaximin in Tablets by HPLC-MS and Microbiological Turbidimetry
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1093/chromsci/bmab044 http://hdl.handle.net/11449/233225 |
Resumo: | Rifaximin, an antimicrobial used for the treatment of various diseases, lacks analytical methods in official compendia for evaluation of the final product. This paper presents an eco-friendly protocol for rifaximin tablets by high performance liquid chromatography coupled with mass spectrometry (HPLC-MS). The method was completely validated according to the International Conference on Harmonization guidelines and developed following the concept of Quality by Design. The separation was achieved using a C18 column, purified water +0.1% glacial acetic acid and ethyl alcohol, 52:48 (v/v), as mobile phase, 0.9 mL min-1 at 290 nm and ambient room temperature. Mass spectral analyses were performed using electrospray ionization (ESI) ion source and ion trap mass analyzer. The method was linear over the concentration range of 5-50 μg mL-1. The sample was subjected to acidic, basic, neutral, oxidative and photolytic degradation. Degradation products did not interfere in the quantification of the rifaximin, so the method can be considered indicative of stability. Degradation products were also evaluated individually by microbiological method using Escherichia coli. The validated method could be used promisingly as green analytical strategies for detection and quantification of rifaximin in tablets. |
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Repositório Institucional da UNESP |
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Eco-Friendly Pharmaceutical Analysis of Rifaximin in Tablets by HPLC-MS and Microbiological TurbidimetryRifaximin, an antimicrobial used for the treatment of various diseases, lacks analytical methods in official compendia for evaluation of the final product. This paper presents an eco-friendly protocol for rifaximin tablets by high performance liquid chromatography coupled with mass spectrometry (HPLC-MS). The method was completely validated according to the International Conference on Harmonization guidelines and developed following the concept of Quality by Design. The separation was achieved using a C18 column, purified water +0.1% glacial acetic acid and ethyl alcohol, 52:48 (v/v), as mobile phase, 0.9 mL min-1 at 290 nm and ambient room temperature. Mass spectral analyses were performed using electrospray ionization (ESI) ion source and ion trap mass analyzer. The method was linear over the concentration range of 5-50 μg mL-1. The sample was subjected to acidic, basic, neutral, oxidative and photolytic degradation. Degradation products did not interfere in the quantification of the rifaximin, so the method can be considered indicative of stability. Degradation products were also evaluated individually by microbiological method using Escherichia coli. The validated method could be used promisingly as green analytical strategies for detection and quantification of rifaximin in tablets.School of Pharmaceutical Sciences São Paulo State University (UNESP), 14800-903 Campus AraraquaraLaboratório de Controle de Qualidade Faculdade de Farmácia Universidade Federal de Goiás - UFGCenter for Research in Natural and Synthetic Products Department of Physics and Chemistry School of Pharmaceutical Sciences of Ribeirão Preto Universidade de São PauloSchool of Pharmaceutical Sciences São Paulo State University (UNESP), 14800-903 Campus AraraquaraUniversidade Estadual Paulista (UNESP)Universidade Federal de Goiás (UFG)Universidade de São Paulo (USP)Kogawa, Ana Carolina [UNESP]Mendonça, Jacqueline NakauLopes, Norberto PeporineSalgado, Hérida Regina Nunes [UNESP]2022-05-01T06:02:10Z2022-05-01T06:02:10Z2021-06-21info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article597-605http://dx.doi.org/10.1093/chromsci/bmab044Journal of chromatographic science, v. 59, n. 7, p. 597-605, 2021.1945-239Xhttp://hdl.handle.net/11449/23322510.1093/chromsci/bmab0442-s2.0-85109115731Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of chromatographic scienceinfo:eu-repo/semantics/openAccess2024-06-24T13:45:50Zoai:repositorio.unesp.br:11449/233225Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:39:12.003177Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Eco-Friendly Pharmaceutical Analysis of Rifaximin in Tablets by HPLC-MS and Microbiological Turbidimetry |
title |
Eco-Friendly Pharmaceutical Analysis of Rifaximin in Tablets by HPLC-MS and Microbiological Turbidimetry |
spellingShingle |
Eco-Friendly Pharmaceutical Analysis of Rifaximin in Tablets by HPLC-MS and Microbiological Turbidimetry Kogawa, Ana Carolina [UNESP] |
title_short |
Eco-Friendly Pharmaceutical Analysis of Rifaximin in Tablets by HPLC-MS and Microbiological Turbidimetry |
title_full |
Eco-Friendly Pharmaceutical Analysis of Rifaximin in Tablets by HPLC-MS and Microbiological Turbidimetry |
title_fullStr |
Eco-Friendly Pharmaceutical Analysis of Rifaximin in Tablets by HPLC-MS and Microbiological Turbidimetry |
title_full_unstemmed |
Eco-Friendly Pharmaceutical Analysis of Rifaximin in Tablets by HPLC-MS and Microbiological Turbidimetry |
title_sort |
Eco-Friendly Pharmaceutical Analysis of Rifaximin in Tablets by HPLC-MS and Microbiological Turbidimetry |
author |
Kogawa, Ana Carolina [UNESP] |
author_facet |
Kogawa, Ana Carolina [UNESP] Mendonça, Jacqueline Nakau Lopes, Norberto Peporine Salgado, Hérida Regina Nunes [UNESP] |
author_role |
author |
author2 |
Mendonça, Jacqueline Nakau Lopes, Norberto Peporine Salgado, Hérida Regina Nunes [UNESP] |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Universidade Federal de Goiás (UFG) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Kogawa, Ana Carolina [UNESP] Mendonça, Jacqueline Nakau Lopes, Norberto Peporine Salgado, Hérida Regina Nunes [UNESP] |
description |
Rifaximin, an antimicrobial used for the treatment of various diseases, lacks analytical methods in official compendia for evaluation of the final product. This paper presents an eco-friendly protocol for rifaximin tablets by high performance liquid chromatography coupled with mass spectrometry (HPLC-MS). The method was completely validated according to the International Conference on Harmonization guidelines and developed following the concept of Quality by Design. The separation was achieved using a C18 column, purified water +0.1% glacial acetic acid and ethyl alcohol, 52:48 (v/v), as mobile phase, 0.9 mL min-1 at 290 nm and ambient room temperature. Mass spectral analyses were performed using electrospray ionization (ESI) ion source and ion trap mass analyzer. The method was linear over the concentration range of 5-50 μg mL-1. The sample was subjected to acidic, basic, neutral, oxidative and photolytic degradation. Degradation products did not interfere in the quantification of the rifaximin, so the method can be considered indicative of stability. Degradation products were also evaluated individually by microbiological method using Escherichia coli. The validated method could be used promisingly as green analytical strategies for detection and quantification of rifaximin in tablets. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-21 2022-05-01T06:02:10Z 2022-05-01T06:02:10Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1093/chromsci/bmab044 Journal of chromatographic science, v. 59, n. 7, p. 597-605, 2021. 1945-239X http://hdl.handle.net/11449/233225 10.1093/chromsci/bmab044 2-s2.0-85109115731 |
url |
http://dx.doi.org/10.1093/chromsci/bmab044 http://hdl.handle.net/11449/233225 |
identifier_str_mv |
Journal of chromatographic science, v. 59, n. 7, p. 597-605, 2021. 1945-239X 10.1093/chromsci/bmab044 2-s2.0-85109115731 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of chromatographic science |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
597-605 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128960845840384 |