Neovascularization after ischemic injury: evaluation with 99mTc-HYNIC-RGD

Detalhes bibliográficos
Autor(a) principal: Faintuch, Bluma Linkowski
Data de Publicação: 2011
Outros Autores: Teodoro, Rodrigo, Oliveira, Erica Aparecida De, Nuñez, Eutimio Gustavo Fernández, Faintuch, Joel
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S0102-86502011000100011
http://hdl.handle.net/11449/127257
Resumo: Purpose: Angiogenesis involves many mediators including integrins, and the tripeptide RGD is a target amino acid recognition sequence for many of them. Hindlimb ischemia is a simple and convenient animal model however standardization of the injection procedures in the devascularized and control limb is lacking, thus rendering difficult the interpretation of results. The aim of this investigations was to evaluate neovascularization in a hindlimb murine model by means of 99mTc-HYNIC-ß-Ala-RGD. Methods: 99mTc-HYNIC-RGD analog was prepared using coligands. Ischemia was induced in Wistar rats by double- ligation of the common femoral artery. Radiolabeled RGD was injected after 2h, as well as 1, 3, 5, 7, 10 and 14 days. Uptake was evaluated by planar imaging and biodistribution studies. Results: The highest ratio between ischemia and control was achieved at the 7th day (2.62 ± 0.95), with substantial decrease by the 14th day. For pertechnetate the 7th day ratio was 0.87 ± 0.23. Scintigraphic image confirmed different uptakes. Conclusion: 99mTc-HYNIC-RGD analog concentrated in ischemic tissue by the time of widespread angiogenesis and pertechnetate confirmed reduction in blood flow. In this sense, the protocol can be recommended for ischemic models.
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spelling Neovascularization after ischemic injury: evaluation with 99mTc-HYNIC-RGDNeovascularização após lesão isquêmica: avaliação com 99mTc-HYNIC-RGDTechnetiumIsotope LabelingRadionuclide ImagingIschemiaDiagnosisRatsTecnécioMarcação por IsótopoCintilografiaIsquemiaDiagnósticoRatosPurpose: Angiogenesis involves many mediators including integrins, and the tripeptide RGD is a target amino acid recognition sequence for many of them. Hindlimb ischemia is a simple and convenient animal model however standardization of the injection procedures in the devascularized and control limb is lacking, thus rendering difficult the interpretation of results. The aim of this investigations was to evaluate neovascularization in a hindlimb murine model by means of 99mTc-HYNIC-ß-Ala-RGD. Methods: 99mTc-HYNIC-RGD analog was prepared using coligands. Ischemia was induced in Wistar rats by double- ligation of the common femoral artery. Radiolabeled RGD was injected after 2h, as well as 1, 3, 5, 7, 10 and 14 days. Uptake was evaluated by planar imaging and biodistribution studies. Results: The highest ratio between ischemia and control was achieved at the 7th day (2.62 ± 0.95), with substantial decrease by the 14th day. For pertechnetate the 7th day ratio was 0.87 ± 0.23. Scintigraphic image confirmed different uptakes. Conclusion: 99mTc-HYNIC-RGD analog concentrated in ischemic tissue by the time of widespread angiogenesis and pertechnetate confirmed reduction in blood flow. In this sense, the protocol can be recommended for ischemic models.Objetivo: A angiogênese em resposta a fenômenos isquêmicos envolve vários mediadores como as integrinas, sendo que o tripeptídeo RGD possui uma seqüência de aminoácidos com reconhecimento para este alvo. O modelo animal de isquemia de pata traseira é simples e conveniente, porém não há uma padronização do procedimento de injeção e controle radioisotópico em membro desvascularizado, dificultando, portanto a interpretação de resultados. O objetivo deste estudo foi avaliar a neovascularização em modelo murino de isquemia de pata traseira através do radiotraçador 99mTc-HYNIC-ß-Ala-RGD. Métodos: O análogo 99mTc-HYNIC-RGD foi preparado usando coligantes. A isquemia foi induzida em ratos Wistar por dupla-ligação da artéria femoral comum na prega inguinal. Peptídeo RGD radiomarcado foi injetado após 2h, assim como 1, 3, 5, 7, 10 e 14 dias. A captação foi avaliada por imagem planar e estudos de biodistribuição. Resultados: A maior diferença de captação entre isquemia e pata controle foi obtida no 7º dia (2,62 ± 0,95), com decréscimo acentuado no 14º dia. Para o pertecnetato a razão no 7º dia foi 0,87 ± 0,23. A imagem cintilográfica confirmou as diferentes captações. Conclusões: O análogo 99mTc-HYNIC-RGD concentrou-se no tecido isquêmico na etapa em que a angiogênese é mais acentuada, e o estudo do pertecnetato confirmou a redução no fluxo sanguíneo. Desta maneira, este protocolo diagnóstico pode ser recomendado para modelos isquêmicos.University of Sao Paulo IPEN Radiopharmacy CenterUSP IPEN Radiopharmacy CenterUSP IPEN Postgraduate ProgramUSP Medical School Department of GastroenterologySociedade Brasileira para o Desenvolvimento da Pesquisa em CirurgiaUniversidade de São Paulo (USP)Faintuch, Bluma LinkowskiTeodoro, RodrigoOliveira, Erica Aparecida DeNuñez, Eutimio Gustavo FernándezFaintuch, Joel2015-08-21T17:54:44Z2015-08-21T17:54:44Z2011-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article58-63application/pdfhttp://dx.doi.org/10.1590/S0102-86502011000100011Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 26, n. 1, p. 58-63, 2011.0102-8650http://hdl.handle.net/11449/12725710.1590/S0102-86502011000100011S0102-86502011000100011S0102-86502011000100011.pdfSciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengActa Cirurgica Brasileira0.9330,395info:eu-repo/semantics/openAccess2023-12-23T06:19:18Zoai:repositorio.unesp.br:11449/127257Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:06:09.832147Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Neovascularization after ischemic injury: evaluation with 99mTc-HYNIC-RGD
Neovascularização após lesão isquêmica: avaliação com 99mTc-HYNIC-RGD
title Neovascularization after ischemic injury: evaluation with 99mTc-HYNIC-RGD
spellingShingle Neovascularization after ischemic injury: evaluation with 99mTc-HYNIC-RGD
Faintuch, Bluma Linkowski
Technetium
Isotope Labeling
Radionuclide Imaging
Ischemia
Diagnosis
Rats
Tecnécio
Marcação por Isótopo
Cintilografia
Isquemia
Diagnóstico
Ratos
title_short Neovascularization after ischemic injury: evaluation with 99mTc-HYNIC-RGD
title_full Neovascularization after ischemic injury: evaluation with 99mTc-HYNIC-RGD
title_fullStr Neovascularization after ischemic injury: evaluation with 99mTc-HYNIC-RGD
title_full_unstemmed Neovascularization after ischemic injury: evaluation with 99mTc-HYNIC-RGD
title_sort Neovascularization after ischemic injury: evaluation with 99mTc-HYNIC-RGD
author Faintuch, Bluma Linkowski
author_facet Faintuch, Bluma Linkowski
Teodoro, Rodrigo
Oliveira, Erica Aparecida De
Nuñez, Eutimio Gustavo Fernández
Faintuch, Joel
author_role author
author2 Teodoro, Rodrigo
Oliveira, Erica Aparecida De
Nuñez, Eutimio Gustavo Fernández
Faintuch, Joel
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv Faintuch, Bluma Linkowski
Teodoro, Rodrigo
Oliveira, Erica Aparecida De
Nuñez, Eutimio Gustavo Fernández
Faintuch, Joel
dc.subject.por.fl_str_mv Technetium
Isotope Labeling
Radionuclide Imaging
Ischemia
Diagnosis
Rats
Tecnécio
Marcação por Isótopo
Cintilografia
Isquemia
Diagnóstico
Ratos
topic Technetium
Isotope Labeling
Radionuclide Imaging
Ischemia
Diagnosis
Rats
Tecnécio
Marcação por Isótopo
Cintilografia
Isquemia
Diagnóstico
Ratos
description Purpose: Angiogenesis involves many mediators including integrins, and the tripeptide RGD is a target amino acid recognition sequence for many of them. Hindlimb ischemia is a simple and convenient animal model however standardization of the injection procedures in the devascularized and control limb is lacking, thus rendering difficult the interpretation of results. The aim of this investigations was to evaluate neovascularization in a hindlimb murine model by means of 99mTc-HYNIC-ß-Ala-RGD. Methods: 99mTc-HYNIC-RGD analog was prepared using coligands. Ischemia was induced in Wistar rats by double- ligation of the common femoral artery. Radiolabeled RGD was injected after 2h, as well as 1, 3, 5, 7, 10 and 14 days. Uptake was evaluated by planar imaging and biodistribution studies. Results: The highest ratio between ischemia and control was achieved at the 7th day (2.62 ± 0.95), with substantial decrease by the 14th day. For pertechnetate the 7th day ratio was 0.87 ± 0.23. Scintigraphic image confirmed different uptakes. Conclusion: 99mTc-HYNIC-RGD analog concentrated in ischemic tissue by the time of widespread angiogenesis and pertechnetate confirmed reduction in blood flow. In this sense, the protocol can be recommended for ischemic models.
publishDate 2011
dc.date.none.fl_str_mv 2011-02-01
2015-08-21T17:54:44Z
2015-08-21T17:54:44Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S0102-86502011000100011
Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 26, n. 1, p. 58-63, 2011.
0102-8650
http://hdl.handle.net/11449/127257
10.1590/S0102-86502011000100011
S0102-86502011000100011
S0102-86502011000100011.pdf
url http://dx.doi.org/10.1590/S0102-86502011000100011
http://hdl.handle.net/11449/127257
identifier_str_mv Acta Cirurgica Brasileira. Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia, v. 26, n. 1, p. 58-63, 2011.
0102-8650
10.1590/S0102-86502011000100011
S0102-86502011000100011
S0102-86502011000100011.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Acta Cirurgica Brasileira
0.933
0,395
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 58-63
application/pdf
dc.publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
dc.source.none.fl_str_mv SciELO
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808129284002283520

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