Circulating microRNAs expression profile in newly diagnosed and imatinib treated chronic phase–chronic myeloid leukemia

Detalhes bibliográficos
Autor(a) principal: Ferreira, Letícia Antunes Muniz [UNESP]
Data de Publicação: 2018
Outros Autores: Capannacci, Juliana, Hokama, Newton Key [UNESP], Nogueira, Célia Regina [UNESP], Ceccarelli, Michele, Cerulo, Luigi, D’Angelo, Fulvio, de Oliveira Montandon Hokama, Paula [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1080/10428194.2018.1499905
http://hdl.handle.net/11449/176842
Resumo: Chronic myeloid leukemia (CML) is a stem cell derived malignant disorder result of translocation t(9;22)(q34;q11) called Philadelphia chromosome (Ph+). microRNAS (miRNAs) are involved in several biological processes, altering the progression of various pathologies, including CML. This study evaluated whether circulating miRNAs display differential expression profiles in peripheral blood of CML-Chronic Phase (CML-CP) patients newly diagnosed in comparison with CML-CP treated with imatinib. We obtained peripheral blood samples from CML-CP Ph+ patients divided among group 1 (untreated newly diagnosed) and group 2 (treated with imatinib). A pool of total leukocytes from healthy donors was considered as control group. Expression analyses were performed for 768 miRNAs by RT-qPCR array. Bioinformatic tools were used to identify significant pathways and interaction networks. We found 80 deregulated miRNAs between the groups and, according to bioinformatic analysis, they are involved in different pathways, including molecular mechanisms of cancer. The study allows better understanding of disease molecular behavior, and it is useful for possible monitoring CML treatment and prognostic biomarkers identification.
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spelling Circulating microRNAs expression profile in newly diagnosed and imatinib treated chronic phase–chronic myeloid leukemiaBiomarkerschronic phasegene expression regulationimatinib mesylateleukemiamicroRNAsmyeloidPhiladelphia chromosomeChronic myeloid leukemia (CML) is a stem cell derived malignant disorder result of translocation t(9;22)(q34;q11) called Philadelphia chromosome (Ph+). microRNAS (miRNAs) are involved in several biological processes, altering the progression of various pathologies, including CML. This study evaluated whether circulating miRNAs display differential expression profiles in peripheral blood of CML-Chronic Phase (CML-CP) patients newly diagnosed in comparison with CML-CP treated with imatinib. We obtained peripheral blood samples from CML-CP Ph+ patients divided among group 1 (untreated newly diagnosed) and group 2 (treated with imatinib). A pool of total leukocytes from healthy donors was considered as control group. Expression analyses were performed for 768 miRNAs by RT-qPCR array. Bioinformatic tools were used to identify significant pathways and interaction networks. We found 80 deregulated miRNAs between the groups and, according to bioinformatic analysis, they are involved in different pathways, including molecular mechanisms of cancer. The study allows better understanding of disease molecular behavior, and it is useful for possible monitoring CML treatment and prognostic biomarkers identification.Department of Internal Medical São Paulo State University (UNESP-FMB)Laboratory of Molecular Biology Hospital Dr. Amaral CarvalhoDepartment of Science and Technology Sannio UniversityDepartment of Internal Medical São Paulo State University (UNESP-FMB)Universidade Estadual Paulista (Unesp)Hospital Dr. Amaral CarvalhoSannio UniversityFerreira, Letícia Antunes Muniz [UNESP]Capannacci, JulianaHokama, Newton Key [UNESP]Nogueira, Célia Regina [UNESP]Ceccarelli, MicheleCerulo, LuigiD’Angelo, Fulviode Oliveira Montandon Hokama, Paula [UNESP]2018-12-11T17:22:43Z2018-12-11T17:22:43Z2018-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1080/10428194.2018.1499905Leukemia and Lymphoma.1029-24031042-8194http://hdl.handle.net/11449/17684210.1080/10428194.2018.14999052-s2.0-850533452414132731111630799Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengLeukemia and Lymphoma0,9760,976info:eu-repo/semantics/openAccess2024-08-14T17:22:48Zoai:repositorio.unesp.br:11449/176842Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-14T17:22:48Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Circulating microRNAs expression profile in newly diagnosed and imatinib treated chronic phase–chronic myeloid leukemia
title Circulating microRNAs expression profile in newly diagnosed and imatinib treated chronic phase–chronic myeloid leukemia
spellingShingle Circulating microRNAs expression profile in newly diagnosed and imatinib treated chronic phase–chronic myeloid leukemia
Ferreira, Letícia Antunes Muniz [UNESP]
Biomarkers
chronic phase
gene expression regulation
imatinib mesylate
leukemia
microRNAs
myeloid
Philadelphia chromosome
title_short Circulating microRNAs expression profile in newly diagnosed and imatinib treated chronic phase–chronic myeloid leukemia
title_full Circulating microRNAs expression profile in newly diagnosed and imatinib treated chronic phase–chronic myeloid leukemia
title_fullStr Circulating microRNAs expression profile in newly diagnosed and imatinib treated chronic phase–chronic myeloid leukemia
title_full_unstemmed Circulating microRNAs expression profile in newly diagnosed and imatinib treated chronic phase–chronic myeloid leukemia
title_sort Circulating microRNAs expression profile in newly diagnosed and imatinib treated chronic phase–chronic myeloid leukemia
author Ferreira, Letícia Antunes Muniz [UNESP]
author_facet Ferreira, Letícia Antunes Muniz [UNESP]
Capannacci, Juliana
Hokama, Newton Key [UNESP]
Nogueira, Célia Regina [UNESP]
Ceccarelli, Michele
Cerulo, Luigi
D’Angelo, Fulvio
de Oliveira Montandon Hokama, Paula [UNESP]
author_role author
author2 Capannacci, Juliana
Hokama, Newton Key [UNESP]
Nogueira, Célia Regina [UNESP]
Ceccarelli, Michele
Cerulo, Luigi
D’Angelo, Fulvio
de Oliveira Montandon Hokama, Paula [UNESP]
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Hospital Dr. Amaral Carvalho
Sannio University
dc.contributor.author.fl_str_mv Ferreira, Letícia Antunes Muniz [UNESP]
Capannacci, Juliana
Hokama, Newton Key [UNESP]
Nogueira, Célia Regina [UNESP]
Ceccarelli, Michele
Cerulo, Luigi
D’Angelo, Fulvio
de Oliveira Montandon Hokama, Paula [UNESP]
dc.subject.por.fl_str_mv Biomarkers
chronic phase
gene expression regulation
imatinib mesylate
leukemia
microRNAs
myeloid
Philadelphia chromosome
topic Biomarkers
chronic phase
gene expression regulation
imatinib mesylate
leukemia
microRNAs
myeloid
Philadelphia chromosome
description Chronic myeloid leukemia (CML) is a stem cell derived malignant disorder result of translocation t(9;22)(q34;q11) called Philadelphia chromosome (Ph+). microRNAS (miRNAs) are involved in several biological processes, altering the progression of various pathologies, including CML. This study evaluated whether circulating miRNAs display differential expression profiles in peripheral blood of CML-Chronic Phase (CML-CP) patients newly diagnosed in comparison with CML-CP treated with imatinib. We obtained peripheral blood samples from CML-CP Ph+ patients divided among group 1 (untreated newly diagnosed) and group 2 (treated with imatinib). A pool of total leukocytes from healthy donors was considered as control group. Expression analyses were performed for 768 miRNAs by RT-qPCR array. Bioinformatic tools were used to identify significant pathways and interaction networks. We found 80 deregulated miRNAs between the groups and, according to bioinformatic analysis, they are involved in different pathways, including molecular mechanisms of cancer. The study allows better understanding of disease molecular behavior, and it is useful for possible monitoring CML treatment and prognostic biomarkers identification.
publishDate 2018
dc.date.none.fl_str_mv 2018-12-11T17:22:43Z
2018-12-11T17:22:43Z
2018-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1080/10428194.2018.1499905
Leukemia and Lymphoma.
1029-2403
1042-8194
http://hdl.handle.net/11449/176842
10.1080/10428194.2018.1499905
2-s2.0-85053345241
4132731111630799
url http://dx.doi.org/10.1080/10428194.2018.1499905
http://hdl.handle.net/11449/176842
identifier_str_mv Leukemia and Lymphoma.
1029-2403
1042-8194
10.1080/10428194.2018.1499905
2-s2.0-85053345241
4132731111630799
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Leukemia and Lymphoma
0,976
0,976
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808128136655667200