Chitosan-based biomaterials used in critical-size bone defects: radiographic study in rat's calvaria

Detalhes bibliográficos
Autor(a) principal: Spin-neto, Rubens
Data de Publicação: 2012
Outros Autores: Coletti, Felipe Leite [UNESP], Freitas, Rubens Moreno De [UNESP], Pavone, Chaíne [UNESP], Campana-filho, Sérgio Paulo, Marcantonio, Rosemary Adriana Chiérici [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: https://scielo.br/article/S1807-25772012000500003
http://hdl.handle.net/11449/213140
Resumo: OBJECTIVE: This study evaluated, using digital radiographic images, the action of chitosan and chitosan hydrochloride biomaterials, with both low and high molecular weight, used in the correction of critical-size bone defects (CSBD's) in rat's calvaria. MATERIAL AND METHOD: CSBD's with 8 mm in diameter were surgically created in the calvaria of 50 Holtzman rats and these were filled with a blood clot (Control), low molecular weight chitosan, high molecular weight chitosan, low molecular weight chitosan hydrochloride and high molecular weight chitosan hydrochloride, for a total of 10 animals, which were divided into two experimental periods (15 and 60 days), for each biomaterial. The radiographic evaluation was made using two digital radiographs of the animal's skull: one taken right after the bone defect was created and the other at the moment of the sacrifice, providing the initial and the final radiographic bone density in the area of the defect, which were compared. RESULT: Analysis of radiographic bone density indicated that the increase in the radiographic bone density of the CSBD's treated with the proposed biomaterials, in either molecular weight, in both observed periods, where similar to those found in control group. CONCLUSION: Tested chitosan-based biomaterials were not able to enhance the radiographic density in the CSBD's made in rat's calvaria.
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spelling Chitosan-based biomaterials used in critical-size bone defects: radiographic study in rat's calvariaBiomateriais a base de quitosana na correção de defeitos ósseos críticos criados em calvaria de ratos: avaliação radiográficaBiocompatible materialsbone regenerationchitosanchitinradiographic image interpretation computer-assistedMateriais biocompatíveisregeneração ósseaquitosanaquitinainterpretação de imagem radiográfica assistida por computadorOBJECTIVE: This study evaluated, using digital radiographic images, the action of chitosan and chitosan hydrochloride biomaterials, with both low and high molecular weight, used in the correction of critical-size bone defects (CSBD's) in rat's calvaria. MATERIAL AND METHOD: CSBD's with 8 mm in diameter were surgically created in the calvaria of 50 Holtzman rats and these were filled with a blood clot (Control), low molecular weight chitosan, high molecular weight chitosan, low molecular weight chitosan hydrochloride and high molecular weight chitosan hydrochloride, for a total of 10 animals, which were divided into two experimental periods (15 and 60 days), for each biomaterial. The radiographic evaluation was made using two digital radiographs of the animal's skull: one taken right after the bone defect was created and the other at the moment of the sacrifice, providing the initial and the final radiographic bone density in the area of the defect, which were compared. RESULT: Analysis of radiographic bone density indicated that the increase in the radiographic bone density of the CSBD's treated with the proposed biomaterials, in either molecular weight, in both observed periods, where similar to those found in control group. CONCLUSION: Tested chitosan-based biomaterials were not able to enhance the radiographic density in the CSBD's made in rat's calvaria.OBJETIVO: Este estudo avaliou através de imagens radiográficas digitais, a ação de biomateriais de quitosana e de cloridrato de quitosana, com baixo e alto peso molecular, utilizados na correção de defeitos ósseos de tamanho crítico (DOTC)em calvária de ratos. MATERIAL E MÉTODO: DOTCs com 8 mm de diâmetro foram criados cirurgicamente na calvária de 50 ratos Holtzman. Em 10 animais o defeito foi preenchido foram preenchidos com coágulo sanguíneo (controle negativo). Os 40 animais restantes foram divididos de acordo com o biomaterial utilizado no preenchimento do defeito (quitosana de baixo peso e de alto peso molecular, e cloridrato de quitosana de baixo e de alto peso molecular), e foram avaliados em dois períodos experimentais (15 e 60 dias), totalizando 5 animais/biomaterial/período de avaliação. RESULTADO: A avaliação radiográfica foi feita utilizando duas radiografias digitais do crânio do animal: uma tomada logo após o defeito ósseo ser criado e a outra no momento do sacrifício. Nessas imagens, foi avaliada a densidade óssea radiográfica inicial e a final na área do defeito, que foram comparadas. As análises na densidade óssea radiográfica indicaram aumento da densidade óssea radiográfica dos DOTCs tratados para todos os biomateriais testados, em ambos os períodos. Resultados semelhantes foram encontrados no grupo controle. CONCLUSÃO: Conclui-se que os biomateriais de quitosana testados não foram capazes de aumentar a densidade radiográfica em DOTC realizados em calvária de ratos.Aarhus University, Department of DentistrySão Paulo State University, Araraquara Dental SchoolUniversity of São Paulo, São Carlos Chemistry InstituteSão Paulo State University, Araraquara Dental SchoolUniversidade Estadual Paulista Júlio de Mesquita FilhoAarhus UniversityUniversidade Estadual Paulista (Unesp)University of São PauloSpin-neto, RubensColetti, Felipe Leite [UNESP]Freitas, Rubens Moreno De [UNESP]Pavone, Chaíne [UNESP]Campana-filho, Sérgio PauloMarcantonio, Rosemary Adriana Chiérici [UNESP]2021-07-14T10:50:38Z2021-07-14T10:50:38Z2012-10info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article312-317application/pdfhttps://scielo.br/article/S1807-25772012000500003Revista de Odontologia da UNESP. Araraquara, SP, Brazil: Universidade Estadual Paulista Júlio de Mesquita Filho, v. 41, n. 5, p. 312-317, 2012.0101-17741807-2577http://hdl.handle.net/11449/213140S1807-25772012000500003S1807-25772012000500003.pdfSciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista de Odontologia da UNESPinfo:eu-repo/semantics/openAccess2024-09-26T15:21:10Zoai:repositorio.unesp.br:11449/213140Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-26T15:21:10Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Chitosan-based biomaterials used in critical-size bone defects: radiographic study in rat's calvaria
Biomateriais a base de quitosana na correção de defeitos ósseos críticos criados em calvaria de ratos: avaliação radiográfica
title Chitosan-based biomaterials used in critical-size bone defects: radiographic study in rat's calvaria
spellingShingle Chitosan-based biomaterials used in critical-size bone defects: radiographic study in rat's calvaria
Spin-neto, Rubens
Biocompatible materials
bone regeneration
chitosan
chitin
radiographic image interpretation computer-assisted
Materiais biocompatíveis
regeneração óssea
quitosana
quitina
interpretação de imagem radiográfica assistida por computador
title_short Chitosan-based biomaterials used in critical-size bone defects: radiographic study in rat's calvaria
title_full Chitosan-based biomaterials used in critical-size bone defects: radiographic study in rat's calvaria
title_fullStr Chitosan-based biomaterials used in critical-size bone defects: radiographic study in rat's calvaria
title_full_unstemmed Chitosan-based biomaterials used in critical-size bone defects: radiographic study in rat's calvaria
title_sort Chitosan-based biomaterials used in critical-size bone defects: radiographic study in rat's calvaria
author Spin-neto, Rubens
author_facet Spin-neto, Rubens
Coletti, Felipe Leite [UNESP]
Freitas, Rubens Moreno De [UNESP]
Pavone, Chaíne [UNESP]
Campana-filho, Sérgio Paulo
Marcantonio, Rosemary Adriana Chiérici [UNESP]
author_role author
author2 Coletti, Felipe Leite [UNESP]
Freitas, Rubens Moreno De [UNESP]
Pavone, Chaíne [UNESP]
Campana-filho, Sérgio Paulo
Marcantonio, Rosemary Adriana Chiérici [UNESP]
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Aarhus University
Universidade Estadual Paulista (Unesp)
University of São Paulo
dc.contributor.author.fl_str_mv Spin-neto, Rubens
Coletti, Felipe Leite [UNESP]
Freitas, Rubens Moreno De [UNESP]
Pavone, Chaíne [UNESP]
Campana-filho, Sérgio Paulo
Marcantonio, Rosemary Adriana Chiérici [UNESP]
dc.subject.por.fl_str_mv Biocompatible materials
bone regeneration
chitosan
chitin
radiographic image interpretation computer-assisted
Materiais biocompatíveis
regeneração óssea
quitosana
quitina
interpretação de imagem radiográfica assistida por computador
topic Biocompatible materials
bone regeneration
chitosan
chitin
radiographic image interpretation computer-assisted
Materiais biocompatíveis
regeneração óssea
quitosana
quitina
interpretação de imagem radiográfica assistida por computador
description OBJECTIVE: This study evaluated, using digital radiographic images, the action of chitosan and chitosan hydrochloride biomaterials, with both low and high molecular weight, used in the correction of critical-size bone defects (CSBD's) in rat's calvaria. MATERIAL AND METHOD: CSBD's with 8 mm in diameter were surgically created in the calvaria of 50 Holtzman rats and these were filled with a blood clot (Control), low molecular weight chitosan, high molecular weight chitosan, low molecular weight chitosan hydrochloride and high molecular weight chitosan hydrochloride, for a total of 10 animals, which were divided into two experimental periods (15 and 60 days), for each biomaterial. The radiographic evaluation was made using two digital radiographs of the animal's skull: one taken right after the bone defect was created and the other at the moment of the sacrifice, providing the initial and the final radiographic bone density in the area of the defect, which were compared. RESULT: Analysis of radiographic bone density indicated that the increase in the radiographic bone density of the CSBD's treated with the proposed biomaterials, in either molecular weight, in both observed periods, where similar to those found in control group. CONCLUSION: Tested chitosan-based biomaterials were not able to enhance the radiographic density in the CSBD's made in rat's calvaria.
publishDate 2012
dc.date.none.fl_str_mv 2012-10
2021-07-14T10:50:38Z
2021-07-14T10:50:38Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://scielo.br/article/S1807-25772012000500003
Revista de Odontologia da UNESP. Araraquara, SP, Brazil: Universidade Estadual Paulista Júlio de Mesquita Filho, v. 41, n. 5, p. 312-317, 2012.
0101-1774
1807-2577
http://hdl.handle.net/11449/213140
S1807-25772012000500003
S1807-25772012000500003.pdf
url https://scielo.br/article/S1807-25772012000500003
http://hdl.handle.net/11449/213140
identifier_str_mv Revista de Odontologia da UNESP. Araraquara, SP, Brazil: Universidade Estadual Paulista Júlio de Mesquita Filho, v. 41, n. 5, p. 312-317, 2012.
0101-1774
1807-2577
S1807-25772012000500003
S1807-25772012000500003.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Revista de Odontologia da UNESP
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 312-317
application/pdf
dc.publisher.none.fl_str_mv Universidade Estadual Paulista Júlio de Mesquita Filho
publisher.none.fl_str_mv Universidade Estadual Paulista Júlio de Mesquita Filho
dc.source.none.fl_str_mv SciELO
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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