Bacterial RNA virus MS2 exposure increases the expression of cancer progression genes in the LNCaP prostate cancer cell line
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3892/ol.2023.13672 http://hdl.handle.net/11449/245574 |
Resumo: | Bacteriophages effectively counteract diverse bacterial infections, and their ability to treat most types of cancer has been explored using phage engineering or phage-virus hybrid platforms. In the present study, it was demonstrated that the bacteriophage MS2 can affect the expression of genes associated with the proliferation and survival of LNCaP prostate epithelial cells. LNCaP cells were exposed to bacteriophage MS2 at a concentration of 1x10(7) plaque forming units/ml for 24-48 h. After exposure, various cellular parameters, including cell viability, morphology, and changes in gene expression, were examined. MS2 affected cell viability adversely, reducing viability by 25% in the first 4 h of treatment; however, cell viability recovered within 24-48 h. Similarly, the AKT, androgen receptor, integrin alpha 5, integrin beta 1, MAPK1, MAPK3, STAT3, and peroxisome proliferator-activated receptor-gamma coactivator 1 alpha genes, which are involved in various normal cellular processes and tumor progression, were significantly upregulated, whereas the expression levels of HSP90, ITGB5, ITGB3, HSP27, ITGAV, and PI3K genes were unchanged. Therefore, based on viability and gene expression changes, bacteriophage MS2 severely impaired LNCaP cells by reducing anchorage-dependent survival and androgen signaling. A caveolin-mediated endocytosis mechanism for MS2-mediated signaling in prostate cancer cells was proposed based on reports involving bacteriophages T4, M13, and MS2, and their interactions with LNCaP and PC3 cell lines. |
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Bacterial RNA virus MS2 exposure increases the expression of cancer progression genes in the LNCaP prostate cancer cell lineandrogen receptorgene expressionintegrinbacteriophageMS2Bacteriophages effectively counteract diverse bacterial infections, and their ability to treat most types of cancer has been explored using phage engineering or phage-virus hybrid platforms. In the present study, it was demonstrated that the bacteriophage MS2 can affect the expression of genes associated with the proliferation and survival of LNCaP prostate epithelial cells. LNCaP cells were exposed to bacteriophage MS2 at a concentration of 1x10(7) plaque forming units/ml for 24-48 h. After exposure, various cellular parameters, including cell viability, morphology, and changes in gene expression, were examined. MS2 affected cell viability adversely, reducing viability by 25% in the first 4 h of treatment; however, cell viability recovered within 24-48 h. Similarly, the AKT, androgen receptor, integrin alpha 5, integrin beta 1, MAPK1, MAPK3, STAT3, and peroxisome proliferator-activated receptor-gamma coactivator 1 alpha genes, which are involved in various normal cellular processes and tumor progression, were significantly upregulated, whereas the expression levels of HSP90, ITGB5, ITGB3, HSP27, ITGAV, and PI3K genes were unchanged. Therefore, based on viability and gene expression changes, bacteriophage MS2 severely impaired LNCaP cells by reducing anchorage-dependent survival and androgen signaling. A caveolin-mediated endocytosis mechanism for MS2-mediated signaling in prostate cancer cells was proposed based on reports involving bacteriophages T4, M13, and MS2, and their interactions with LNCaP and PC3 cell lines.Sao Paulo State Univ, Inst Biosci Botucatu, Dept Struct & Funct Biol, Lab Extracellular Matrix Biol, BR-18618689 Botucatu, SP, BrazilEotvos Lorand Res Network, Biol Res Ctr, Synthet & Syst Biol Unit, H-6726 Szeged, HungaryUniv Warsaw, Fac Chem, PL-02093 Warsaw, PolandUniv Estadual Campinas, Inst Biol, Dept Struct & Funct Biol, BR-13083970 Campinas, SP, BrazilSao Paulo State Univ, Fac Med, Dept Surg & Orthoped, BR-18618687 Botucatu, SP, BrazilSao Paulo State Univ, Inst Biosci Botucatu, Dept Struct & Funct Biol, Lab Extracellular Matrix Biol, 250 Antonio Celso Wagner Zanin, BR-18618689 Botucatu, SP, BrazilSao Paulo State Univ, Inst Biosci Botucatu, Dept Struct & Funct Biol, Lab Extracellular Matrix Biol, BR-18618689 Botucatu, SP, BrazilSao Paulo State Univ, Fac Med, Dept Surg & Orthoped, BR-18618687 Botucatu, SP, BrazilSao Paulo State Univ, Inst Biosci Botucatu, Dept Struct & Funct Biol, Lab Extracellular Matrix Biol, 250 Antonio Celso Wagner Zanin, BR-18618689 Botucatu, SP, BrazilSpandidos Publ LtdUniversidade Estadual Paulista (UNESP)Eotvos Lorand Res NetworkUniv WarsawUniversidade Estadual de Campinas (UNICAMP)Sanmukh, Swapnil Ganesh [UNESP]Santos, Nilton Jose dos [UNESP]Barquilha, Caroline Nascimento [UNESP]Carvalho, Marcio de [UNESP]Reis, Patricia Pintor dos [UNESP]Delella, Flavia Karina [UNESP]Carvalho, Hernandes F. F.Latek, DorotaFeher, TamasFelisbino, Sergio Luis [UNESP]2023-07-29T11:58:48Z2023-07-29T11:58:48Z2023-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article14http://dx.doi.org/10.3892/ol.2023.13672Oncology Letters. Athens: Spandidos Publ Ltd, v. 25, n. 2, 14 p., 2023.1792-1074http://hdl.handle.net/11449/24557410.3892/ol.2023.13672WOS:000918372700001Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengOncology Lettersinfo:eu-repo/semantics/openAccess2023-07-29T11:58:48Zoai:repositorio.unesp.br:11449/245574Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-07-29T11:58:48Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Bacterial RNA virus MS2 exposure increases the expression of cancer progression genes in the LNCaP prostate cancer cell line |
title |
Bacterial RNA virus MS2 exposure increases the expression of cancer progression genes in the LNCaP prostate cancer cell line |
spellingShingle |
Bacterial RNA virus MS2 exposure increases the expression of cancer progression genes in the LNCaP prostate cancer cell line Sanmukh, Swapnil Ganesh [UNESP] androgen receptor gene expression integrin bacteriophage MS2 |
title_short |
Bacterial RNA virus MS2 exposure increases the expression of cancer progression genes in the LNCaP prostate cancer cell line |
title_full |
Bacterial RNA virus MS2 exposure increases the expression of cancer progression genes in the LNCaP prostate cancer cell line |
title_fullStr |
Bacterial RNA virus MS2 exposure increases the expression of cancer progression genes in the LNCaP prostate cancer cell line |
title_full_unstemmed |
Bacterial RNA virus MS2 exposure increases the expression of cancer progression genes in the LNCaP prostate cancer cell line |
title_sort |
Bacterial RNA virus MS2 exposure increases the expression of cancer progression genes in the LNCaP prostate cancer cell line |
author |
Sanmukh, Swapnil Ganesh [UNESP] |
author_facet |
Sanmukh, Swapnil Ganesh [UNESP] Santos, Nilton Jose dos [UNESP] Barquilha, Caroline Nascimento [UNESP] Carvalho, Marcio de [UNESP] Reis, Patricia Pintor dos [UNESP] Delella, Flavia Karina [UNESP] Carvalho, Hernandes F. F. Latek, Dorota Feher, Tamas Felisbino, Sergio Luis [UNESP] |
author_role |
author |
author2 |
Santos, Nilton Jose dos [UNESP] Barquilha, Caroline Nascimento [UNESP] Carvalho, Marcio de [UNESP] Reis, Patricia Pintor dos [UNESP] Delella, Flavia Karina [UNESP] Carvalho, Hernandes F. F. Latek, Dorota Feher, Tamas Felisbino, Sergio Luis [UNESP] |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Eotvos Lorand Res Network Univ Warsaw Universidade Estadual de Campinas (UNICAMP) |
dc.contributor.author.fl_str_mv |
Sanmukh, Swapnil Ganesh [UNESP] Santos, Nilton Jose dos [UNESP] Barquilha, Caroline Nascimento [UNESP] Carvalho, Marcio de [UNESP] Reis, Patricia Pintor dos [UNESP] Delella, Flavia Karina [UNESP] Carvalho, Hernandes F. F. Latek, Dorota Feher, Tamas Felisbino, Sergio Luis [UNESP] |
dc.subject.por.fl_str_mv |
androgen receptor gene expression integrin bacteriophage MS2 |
topic |
androgen receptor gene expression integrin bacteriophage MS2 |
description |
Bacteriophages effectively counteract diverse bacterial infections, and their ability to treat most types of cancer has been explored using phage engineering or phage-virus hybrid platforms. In the present study, it was demonstrated that the bacteriophage MS2 can affect the expression of genes associated with the proliferation and survival of LNCaP prostate epithelial cells. LNCaP cells were exposed to bacteriophage MS2 at a concentration of 1x10(7) plaque forming units/ml for 24-48 h. After exposure, various cellular parameters, including cell viability, morphology, and changes in gene expression, were examined. MS2 affected cell viability adversely, reducing viability by 25% in the first 4 h of treatment; however, cell viability recovered within 24-48 h. Similarly, the AKT, androgen receptor, integrin alpha 5, integrin beta 1, MAPK1, MAPK3, STAT3, and peroxisome proliferator-activated receptor-gamma coactivator 1 alpha genes, which are involved in various normal cellular processes and tumor progression, were significantly upregulated, whereas the expression levels of HSP90, ITGB5, ITGB3, HSP27, ITGAV, and PI3K genes were unchanged. Therefore, based on viability and gene expression changes, bacteriophage MS2 severely impaired LNCaP cells by reducing anchorage-dependent survival and androgen signaling. A caveolin-mediated endocytosis mechanism for MS2-mediated signaling in prostate cancer cells was proposed based on reports involving bacteriophages T4, M13, and MS2, and their interactions with LNCaP and PC3 cell lines. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-07-29T11:58:48Z 2023-07-29T11:58:48Z 2023-02-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3892/ol.2023.13672 Oncology Letters. Athens: Spandidos Publ Ltd, v. 25, n. 2, 14 p., 2023. 1792-1074 http://hdl.handle.net/11449/245574 10.3892/ol.2023.13672 WOS:000918372700001 |
url |
http://dx.doi.org/10.3892/ol.2023.13672 http://hdl.handle.net/11449/245574 |
identifier_str_mv |
Oncology Letters. Athens: Spandidos Publ Ltd, v. 25, n. 2, 14 p., 2023. 1792-1074 10.3892/ol.2023.13672 WOS:000918372700001 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Oncology Letters |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
14 |
dc.publisher.none.fl_str_mv |
Spandidos Publ Ltd |
publisher.none.fl_str_mv |
Spandidos Publ Ltd |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965408172703744 |