Physical exercise increases Sestrin 2 protein levels and induces autophagy in the skeletal muscle of old mice
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.exger.2017.07.009 http://hdl.handle.net/11449/159739 |
Resumo: | Sestrins and autophagy deficiencies are associated with several aging-related organic dysfunctions and metabolic disorders. Here we evaluate the effects of acute exercise on Sestrin 2 (Sesn2) protein content and autophagy markers in the skeletal muscle of experimental models of aging. Twenty-four months-old C57BL/6 J male mice were submitted to a single bout of swimming exercise and the gastrocnemius muscle was evaluated by Western blot. Transcriptomic and phenotypic analysis were also performed by using strains of genetically-diverse BXD mice. The bioinformatics analysis showed a negative correlation between Sesn2 mRNA levels in the skeletal muscle and body weight gain, plasma triglycerides and fasting glucose and positive correlation with several autophagic markers in the muscle of BXDmice. Consistent with these findings, low levels of Sesn2 protein content were observed in the gastrocnemius muscle of C57BL/6 J old mice when compared to young group. Interestingly, the acute aerobic exercise induced Sesn2 accumulation and modulated several markers of autophagy in the gastrocnemius muscle old mice, including unc-51-like kinase-1 (Ulk1) phosphorylation and the protein levels of Atg5, Atg7, p62 and LC3-II. Finally, exercise increased insulin sensitivity in old animals, as demonstrated by kITT. Taken together, these findings demonstrated the acutely, aerobic physical exercise recovers Sestrin 2 protein content and induces autophagy in the skeletal muscle of old mice, contributing with the improvement of insulin sensitivity an aging animal model. (C) 2017 Elsevier Inc. All rights reserved. |
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Physical exercise increases Sestrin 2 protein levels and induces autophagy in the skeletal muscle of old micePhysical exerciseAgingSestrin 2AutophagySkeletal muscleSestrins and autophagy deficiencies are associated with several aging-related organic dysfunctions and metabolic disorders. Here we evaluate the effects of acute exercise on Sestrin 2 (Sesn2) protein content and autophagy markers in the skeletal muscle of experimental models of aging. Twenty-four months-old C57BL/6 J male mice were submitted to a single bout of swimming exercise and the gastrocnemius muscle was evaluated by Western blot. Transcriptomic and phenotypic analysis were also performed by using strains of genetically-diverse BXD mice. The bioinformatics analysis showed a negative correlation between Sesn2 mRNA levels in the skeletal muscle and body weight gain, plasma triglycerides and fasting glucose and positive correlation with several autophagic markers in the muscle of BXDmice. Consistent with these findings, low levels of Sesn2 protein content were observed in the gastrocnemius muscle of C57BL/6 J old mice when compared to young group. Interestingly, the acute aerobic exercise induced Sesn2 accumulation and modulated several markers of autophagy in the gastrocnemius muscle old mice, including unc-51-like kinase-1 (Ulk1) phosphorylation and the protein levels of Atg5, Atg7, p62 and LC3-II. Finally, exercise increased insulin sensitivity in old animals, as demonstrated by kITT. Taken together, these findings demonstrated the acutely, aerobic physical exercise recovers Sestrin 2 protein content and induces autophagy in the skeletal muscle of old mice, contributing with the improvement of insulin sensitivity an aging animal model. (C) 2017 Elsevier Inc. All rights reserved.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Univ Campinas UNICAMP, Sch Appl Sci, Lab Mol Biol Exercise LaBMEx, Limeira, SP, BrazilUniv Campinas UNICAMP, Fac Med Sci, Dept Internal Med, Campinas, SP, BrazilUniv Estadual Campinas, Sch Appl Sci, Biotechnol Lab, BR-13484350 Limeira, SP, BrazilSao Paulo State Univ, Inst Biosci, Rio Claro, SP, BrazilUniv Sao Paulo, Ribeirao Preto Med Sch, Postgrad Program Rehabil & Funct Performance, Ribeirao Preto, SP, BrazilUniv Sao Paulo, Sch Phys Educ & Sport Ribeirao Preto, Ribeirao Preto, SP, BrazilUniv Estadual Campinas, Sch Appl Sci, Lab Nutr Genom LabGeN, Limeira, SP, BrazilUniv Campinas UNICAMP, Sch Appl Sci, CEPECE Ctr Res Sport Sci, Limeira, SP, BrazilSao Paulo State Univ, Inst Biosci, Rio Claro, SP, BrazilFAPESP: FAPESP- 2016/01089-3Elsevier B.V.Universidade Estadual de Campinas (UNICAMP)Universidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Lenhare, LucieneCrisol, Barbara M.Silva, Vagner R. R.Katashima, Carlos K.Cordeiro, Andre V.Pereira, Karina D. [UNESP]Luchessi, Augusto D. [UNESP]Silva, Adelino S. R. daCintra, Dennys E.Moura, Leandro P.Pauli, Jose R.Ropelle, Eduardo R.2018-11-26T15:45:04Z2018-11-26T15:45:04Z2017-10-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article17-21application/pdfhttp://dx.doi.org/10.1016/j.exger.2017.07.009Experimental Gerontology. Oxford: Pergamon-elsevier Science Ltd, v. 97, p. 17-21, 2017.0531-5565http://hdl.handle.net/11449/15973910.1016/j.exger.2017.07.009WOS:000410651200003WOS000410651200003.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengExperimental Gerontology1,450info:eu-repo/semantics/openAccess2023-11-11T06:14:48Zoai:repositorio.unesp.br:11449/159739Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:24:43.442053Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Physical exercise increases Sestrin 2 protein levels and induces autophagy in the skeletal muscle of old mice |
title |
Physical exercise increases Sestrin 2 protein levels and induces autophagy in the skeletal muscle of old mice |
spellingShingle |
Physical exercise increases Sestrin 2 protein levels and induces autophagy in the skeletal muscle of old mice Lenhare, Luciene Physical exercise Aging Sestrin 2 Autophagy Skeletal muscle |
title_short |
Physical exercise increases Sestrin 2 protein levels and induces autophagy in the skeletal muscle of old mice |
title_full |
Physical exercise increases Sestrin 2 protein levels and induces autophagy in the skeletal muscle of old mice |
title_fullStr |
Physical exercise increases Sestrin 2 protein levels and induces autophagy in the skeletal muscle of old mice |
title_full_unstemmed |
Physical exercise increases Sestrin 2 protein levels and induces autophagy in the skeletal muscle of old mice |
title_sort |
Physical exercise increases Sestrin 2 protein levels and induces autophagy in the skeletal muscle of old mice |
author |
Lenhare, Luciene |
author_facet |
Lenhare, Luciene Crisol, Barbara M. Silva, Vagner R. R. Katashima, Carlos K. Cordeiro, Andre V. Pereira, Karina D. [UNESP] Luchessi, Augusto D. [UNESP] Silva, Adelino S. R. da Cintra, Dennys E. Moura, Leandro P. Pauli, Jose R. Ropelle, Eduardo R. |
author_role |
author |
author2 |
Crisol, Barbara M. Silva, Vagner R. R. Katashima, Carlos K. Cordeiro, Andre V. Pereira, Karina D. [UNESP] Luchessi, Augusto D. [UNESP] Silva, Adelino S. R. da Cintra, Dennys E. Moura, Leandro P. Pauli, Jose R. Ropelle, Eduardo R. |
author2_role |
author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual de Campinas (UNICAMP) Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Lenhare, Luciene Crisol, Barbara M. Silva, Vagner R. R. Katashima, Carlos K. Cordeiro, Andre V. Pereira, Karina D. [UNESP] Luchessi, Augusto D. [UNESP] Silva, Adelino S. R. da Cintra, Dennys E. Moura, Leandro P. Pauli, Jose R. Ropelle, Eduardo R. |
dc.subject.por.fl_str_mv |
Physical exercise Aging Sestrin 2 Autophagy Skeletal muscle |
topic |
Physical exercise Aging Sestrin 2 Autophagy Skeletal muscle |
description |
Sestrins and autophagy deficiencies are associated with several aging-related organic dysfunctions and metabolic disorders. Here we evaluate the effects of acute exercise on Sestrin 2 (Sesn2) protein content and autophagy markers in the skeletal muscle of experimental models of aging. Twenty-four months-old C57BL/6 J male mice were submitted to a single bout of swimming exercise and the gastrocnemius muscle was evaluated by Western blot. Transcriptomic and phenotypic analysis were also performed by using strains of genetically-diverse BXD mice. The bioinformatics analysis showed a negative correlation between Sesn2 mRNA levels in the skeletal muscle and body weight gain, plasma triglycerides and fasting glucose and positive correlation with several autophagic markers in the muscle of BXDmice. Consistent with these findings, low levels of Sesn2 protein content were observed in the gastrocnemius muscle of C57BL/6 J old mice when compared to young group. Interestingly, the acute aerobic exercise induced Sesn2 accumulation and modulated several markers of autophagy in the gastrocnemius muscle old mice, including unc-51-like kinase-1 (Ulk1) phosphorylation and the protein levels of Atg5, Atg7, p62 and LC3-II. Finally, exercise increased insulin sensitivity in old animals, as demonstrated by kITT. Taken together, these findings demonstrated the acutely, aerobic physical exercise recovers Sestrin 2 protein content and induces autophagy in the skeletal muscle of old mice, contributing with the improvement of insulin sensitivity an aging animal model. (C) 2017 Elsevier Inc. All rights reserved. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-10-15 2018-11-26T15:45:04Z 2018-11-26T15:45:04Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.exger.2017.07.009 Experimental Gerontology. Oxford: Pergamon-elsevier Science Ltd, v. 97, p. 17-21, 2017. 0531-5565 http://hdl.handle.net/11449/159739 10.1016/j.exger.2017.07.009 WOS:000410651200003 WOS000410651200003.pdf |
url |
http://dx.doi.org/10.1016/j.exger.2017.07.009 http://hdl.handle.net/11449/159739 |
identifier_str_mv |
Experimental Gerontology. Oxford: Pergamon-elsevier Science Ltd, v. 97, p. 17-21, 2017. 0531-5565 10.1016/j.exger.2017.07.009 WOS:000410651200003 WOS000410651200003.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Experimental Gerontology 1,450 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
17-21 application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128806126354432 |