Physical exercise increases Sestrin 2 protein levels and induces autophagy in the skeletal muscle of old mice

Detalhes bibliográficos
Autor(a) principal: Lenhare, Luciene
Data de Publicação: 2017
Outros Autores: Crisol, Barbara M., Silva, Vagner R. R., Katashima, Carlos K., Cordeiro, Andre V., Pereira, Karina D. [UNESP], Luchessi, Augusto D. [UNESP], Silva, Adelino S. R. da, Cintra, Dennys E., Moura, Leandro P., Pauli, Jose R., Ropelle, Eduardo R.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.exger.2017.07.009
http://hdl.handle.net/11449/159739
Resumo: Sestrins and autophagy deficiencies are associated with several aging-related organic dysfunctions and metabolic disorders. Here we evaluate the effects of acute exercise on Sestrin 2 (Sesn2) protein content and autophagy markers in the skeletal muscle of experimental models of aging. Twenty-four months-old C57BL/6 J male mice were submitted to a single bout of swimming exercise and the gastrocnemius muscle was evaluated by Western blot. Transcriptomic and phenotypic analysis were also performed by using strains of genetically-diverse BXD mice. The bioinformatics analysis showed a negative correlation between Sesn2 mRNA levels in the skeletal muscle and body weight gain, plasma triglycerides and fasting glucose and positive correlation with several autophagic markers in the muscle of BXDmice. Consistent with these findings, low levels of Sesn2 protein content were observed in the gastrocnemius muscle of C57BL/6 J old mice when compared to young group. Interestingly, the acute aerobic exercise induced Sesn2 accumulation and modulated several markers of autophagy in the gastrocnemius muscle old mice, including unc-51-like kinase-1 (Ulk1) phosphorylation and the protein levels of Atg5, Atg7, p62 and LC3-II. Finally, exercise increased insulin sensitivity in old animals, as demonstrated by kITT. Taken together, these findings demonstrated the acutely, aerobic physical exercise recovers Sestrin 2 protein content and induces autophagy in the skeletal muscle of old mice, contributing with the improvement of insulin sensitivity an aging animal model. (C) 2017 Elsevier Inc. All rights reserved.
id UNSP_c4be24efa5675c3a707f12313d5c6155
oai_identifier_str oai:repositorio.unesp.br:11449/159739
network_acronym_str UNSP
network_name_str Repositório Institucional da UNESP
repository_id_str 2946
spelling Physical exercise increases Sestrin 2 protein levels and induces autophagy in the skeletal muscle of old micePhysical exerciseAgingSestrin 2AutophagySkeletal muscleSestrins and autophagy deficiencies are associated with several aging-related organic dysfunctions and metabolic disorders. Here we evaluate the effects of acute exercise on Sestrin 2 (Sesn2) protein content and autophagy markers in the skeletal muscle of experimental models of aging. Twenty-four months-old C57BL/6 J male mice were submitted to a single bout of swimming exercise and the gastrocnemius muscle was evaluated by Western blot. Transcriptomic and phenotypic analysis were also performed by using strains of genetically-diverse BXD mice. The bioinformatics analysis showed a negative correlation between Sesn2 mRNA levels in the skeletal muscle and body weight gain, plasma triglycerides and fasting glucose and positive correlation with several autophagic markers in the muscle of BXDmice. Consistent with these findings, low levels of Sesn2 protein content were observed in the gastrocnemius muscle of C57BL/6 J old mice when compared to young group. Interestingly, the acute aerobic exercise induced Sesn2 accumulation and modulated several markers of autophagy in the gastrocnemius muscle old mice, including unc-51-like kinase-1 (Ulk1) phosphorylation and the protein levels of Atg5, Atg7, p62 and LC3-II. Finally, exercise increased insulin sensitivity in old animals, as demonstrated by kITT. Taken together, these findings demonstrated the acutely, aerobic physical exercise recovers Sestrin 2 protein content and induces autophagy in the skeletal muscle of old mice, contributing with the improvement of insulin sensitivity an aging animal model. (C) 2017 Elsevier Inc. All rights reserved.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Univ Campinas UNICAMP, Sch Appl Sci, Lab Mol Biol Exercise LaBMEx, Limeira, SP, BrazilUniv Campinas UNICAMP, Fac Med Sci, Dept Internal Med, Campinas, SP, BrazilUniv Estadual Campinas, Sch Appl Sci, Biotechnol Lab, BR-13484350 Limeira, SP, BrazilSao Paulo State Univ, Inst Biosci, Rio Claro, SP, BrazilUniv Sao Paulo, Ribeirao Preto Med Sch, Postgrad Program Rehabil & Funct Performance, Ribeirao Preto, SP, BrazilUniv Sao Paulo, Sch Phys Educ & Sport Ribeirao Preto, Ribeirao Preto, SP, BrazilUniv Estadual Campinas, Sch Appl Sci, Lab Nutr Genom LabGeN, Limeira, SP, BrazilUniv Campinas UNICAMP, Sch Appl Sci, CEPECE Ctr Res Sport Sci, Limeira, SP, BrazilSao Paulo State Univ, Inst Biosci, Rio Claro, SP, BrazilFAPESP: FAPESP- 2016/01089-3Elsevier B.V.Universidade Estadual de Campinas (UNICAMP)Universidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Lenhare, LucieneCrisol, Barbara M.Silva, Vagner R. R.Katashima, Carlos K.Cordeiro, Andre V.Pereira, Karina D. [UNESP]Luchessi, Augusto D. [UNESP]Silva, Adelino S. R. daCintra, Dennys E.Moura, Leandro P.Pauli, Jose R.Ropelle, Eduardo R.2018-11-26T15:45:04Z2018-11-26T15:45:04Z2017-10-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article17-21application/pdfhttp://dx.doi.org/10.1016/j.exger.2017.07.009Experimental Gerontology. Oxford: Pergamon-elsevier Science Ltd, v. 97, p. 17-21, 2017.0531-5565http://hdl.handle.net/11449/15973910.1016/j.exger.2017.07.009WOS:000410651200003WOS000410651200003.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengExperimental Gerontology1,450info:eu-repo/semantics/openAccess2023-11-11T06:14:48Zoai:repositorio.unesp.br:11449/159739Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:24:43.442053Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Physical exercise increases Sestrin 2 protein levels and induces autophagy in the skeletal muscle of old mice
title Physical exercise increases Sestrin 2 protein levels and induces autophagy in the skeletal muscle of old mice
spellingShingle Physical exercise increases Sestrin 2 protein levels and induces autophagy in the skeletal muscle of old mice
Lenhare, Luciene
Physical exercise
Aging
Sestrin 2
Autophagy
Skeletal muscle
title_short Physical exercise increases Sestrin 2 protein levels and induces autophagy in the skeletal muscle of old mice
title_full Physical exercise increases Sestrin 2 protein levels and induces autophagy in the skeletal muscle of old mice
title_fullStr Physical exercise increases Sestrin 2 protein levels and induces autophagy in the skeletal muscle of old mice
title_full_unstemmed Physical exercise increases Sestrin 2 protein levels and induces autophagy in the skeletal muscle of old mice
title_sort Physical exercise increases Sestrin 2 protein levels and induces autophagy in the skeletal muscle of old mice
author Lenhare, Luciene
author_facet Lenhare, Luciene
Crisol, Barbara M.
Silva, Vagner R. R.
Katashima, Carlos K.
Cordeiro, Andre V.
Pereira, Karina D. [UNESP]
Luchessi, Augusto D. [UNESP]
Silva, Adelino S. R. da
Cintra, Dennys E.
Moura, Leandro P.
Pauli, Jose R.
Ropelle, Eduardo R.
author_role author
author2 Crisol, Barbara M.
Silva, Vagner R. R.
Katashima, Carlos K.
Cordeiro, Andre V.
Pereira, Karina D. [UNESP]
Luchessi, Augusto D. [UNESP]
Silva, Adelino S. R. da
Cintra, Dennys E.
Moura, Leandro P.
Pauli, Jose R.
Ropelle, Eduardo R.
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual de Campinas (UNICAMP)
Universidade Estadual Paulista (Unesp)
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv Lenhare, Luciene
Crisol, Barbara M.
Silva, Vagner R. R.
Katashima, Carlos K.
Cordeiro, Andre V.
Pereira, Karina D. [UNESP]
Luchessi, Augusto D. [UNESP]
Silva, Adelino S. R. da
Cintra, Dennys E.
Moura, Leandro P.
Pauli, Jose R.
Ropelle, Eduardo R.
dc.subject.por.fl_str_mv Physical exercise
Aging
Sestrin 2
Autophagy
Skeletal muscle
topic Physical exercise
Aging
Sestrin 2
Autophagy
Skeletal muscle
description Sestrins and autophagy deficiencies are associated with several aging-related organic dysfunctions and metabolic disorders. Here we evaluate the effects of acute exercise on Sestrin 2 (Sesn2) protein content and autophagy markers in the skeletal muscle of experimental models of aging. Twenty-four months-old C57BL/6 J male mice were submitted to a single bout of swimming exercise and the gastrocnemius muscle was evaluated by Western blot. Transcriptomic and phenotypic analysis were also performed by using strains of genetically-diverse BXD mice. The bioinformatics analysis showed a negative correlation between Sesn2 mRNA levels in the skeletal muscle and body weight gain, plasma triglycerides and fasting glucose and positive correlation with several autophagic markers in the muscle of BXDmice. Consistent with these findings, low levels of Sesn2 protein content were observed in the gastrocnemius muscle of C57BL/6 J old mice when compared to young group. Interestingly, the acute aerobic exercise induced Sesn2 accumulation and modulated several markers of autophagy in the gastrocnemius muscle old mice, including unc-51-like kinase-1 (Ulk1) phosphorylation and the protein levels of Atg5, Atg7, p62 and LC3-II. Finally, exercise increased insulin sensitivity in old animals, as demonstrated by kITT. Taken together, these findings demonstrated the acutely, aerobic physical exercise recovers Sestrin 2 protein content and induces autophagy in the skeletal muscle of old mice, contributing with the improvement of insulin sensitivity an aging animal model. (C) 2017 Elsevier Inc. All rights reserved.
publishDate 2017
dc.date.none.fl_str_mv 2017-10-15
2018-11-26T15:45:04Z
2018-11-26T15:45:04Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.exger.2017.07.009
Experimental Gerontology. Oxford: Pergamon-elsevier Science Ltd, v. 97, p. 17-21, 2017.
0531-5565
http://hdl.handle.net/11449/159739
10.1016/j.exger.2017.07.009
WOS:000410651200003
WOS000410651200003.pdf
url http://dx.doi.org/10.1016/j.exger.2017.07.009
http://hdl.handle.net/11449/159739
identifier_str_mv Experimental Gerontology. Oxford: Pergamon-elsevier Science Ltd, v. 97, p. 17-21, 2017.
0531-5565
10.1016/j.exger.2017.07.009
WOS:000410651200003
WOS000410651200003.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Experimental Gerontology
1,450
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 17-21
application/pdf
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808128806126354432