Systemic and local effects of radiotherapy: an experimental study on implants placed in rats

Detalhes bibliográficos
Autor(a) principal: da Cruz Vegian, Mariana Raquel [UNESP]
Data de Publicação: 2019
Outros Autores: Costa, Bruno César Almeida [UNESP], de Fátima Santana-Melo, Gabriela [UNESP], Godoi, Fernanda Herrera Costa [UNESP], Kaminagakura, Estela [UNESP], Tango, Rubens Nisie [UNESP], do Prado, Renata Falchete [UNESP], de Oliveira, Luciane Dias [UNESP], Federico, Claudio Antonio, de Oliveira Marco Avelino, Sarah [UNESP], Neves, Rafael Marques [UNESP], de Vasconcellos, Luana Marotta Reis [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1007/s00784-019-02946-5
http://hdl.handle.net/11449/189198
Resumo: Objectives: Evaluate the modulating effect of ionizing radiation, blood cytokine levels, and bone remodeling of the interface around the implant to understand the radiation mechanisms which can impair the implants receptor site. Material and methods: Sixty rats were submitted to grade V titanium implants in the femurs and were divided into the following groups: no-irradiation (N-Ir): control group with implant only; early-irradiation (E-Ir): implant + irradiation after 24 h; late-irradiation (L-Ir): implant + irradiation after 4 weeks; and previous-irradiation (P-Ir): irradiation + implant after 4 weeks. The animals in the E-Ir, L-Ir, and P-Ir groups were irradiated in two fractional stages of 15 Gy. At 3 days, 2 weeks, and 7 weeks after the final procedure, five animals were randomly euthanized per group. Serum levels of TNF-ɑ, IL-1β, TGF-β, IL-6, M-CSF, and IL-10 were measured from blood collected prior to euthanasia using the ELISA test. The pieces containing the implants were subjected to immunohistochemical labeling using the tartrate acid resistant to phosphatase, osteocalcin, and caspase-3 markers and mCT. The ANOVA test was used for statistical analysis, and the Tukey multiple comparison test (p < 0.05) was applied. Results: The results indicated that ionizing radiation modifies the production of pro- and anti-inflammatory serum cytokines, the expression of proteins involved in bone remodeling and cellular apoptosis, as well as changes in bone formation. Conclusions: The results suggests that a longer period between radiotherapy and implant placement surgery when irradiation occurs prior to implant installation would allow the recovery and renewal of bone cells and avoid future failures in osseointegration. Clinical relevance: The search for modifications caused by ionizing irradiation in bone tissue can indicate the ideal period for implant placement without affecting the osseointegration process.
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spelling Systemic and local effects of radiotherapy: an experimental study on implants placed in ratsBone remodelingCytokinesImmunohistochemistryRadiotherapyTitanium implantsObjectives: Evaluate the modulating effect of ionizing radiation, blood cytokine levels, and bone remodeling of the interface around the implant to understand the radiation mechanisms which can impair the implants receptor site. Material and methods: Sixty rats were submitted to grade V titanium implants in the femurs and were divided into the following groups: no-irradiation (N-Ir): control group with implant only; early-irradiation (E-Ir): implant + irradiation after 24 h; late-irradiation (L-Ir): implant + irradiation after 4 weeks; and previous-irradiation (P-Ir): irradiation + implant after 4 weeks. The animals in the E-Ir, L-Ir, and P-Ir groups were irradiated in two fractional stages of 15 Gy. At 3 days, 2 weeks, and 7 weeks after the final procedure, five animals were randomly euthanized per group. Serum levels of TNF-ɑ, IL-1β, TGF-β, IL-6, M-CSF, and IL-10 were measured from blood collected prior to euthanasia using the ELISA test. The pieces containing the implants were subjected to immunohistochemical labeling using the tartrate acid resistant to phosphatase, osteocalcin, and caspase-3 markers and mCT. The ANOVA test was used for statistical analysis, and the Tukey multiple comparison test (p < 0.05) was applied. Results: The results indicated that ionizing radiation modifies the production of pro- and anti-inflammatory serum cytokines, the expression of proteins involved in bone remodeling and cellular apoptosis, as well as changes in bone formation. Conclusions: The results suggests that a longer period between radiotherapy and implant placement surgery when irradiation occurs prior to implant installation would allow the recovery and renewal of bone cells and avoid future failures in osseointegration. Clinical relevance: The search for modifications caused by ionizing irradiation in bone tissue can indicate the ideal period for implant placement without affecting the osseointegration process.Department of Bioscience and Oral Diagnosis Institute of Science and Technology of São José dos Campos Sao Paulo State University (Unesp), Av. Engenheiro Francisco José Longo, 777Department of Dental Materials and Prosthodontics Institute of Science and Technology of São José dos Campos Sao Paulo State University (Unesp)Department of Aerospace Science and Technology Institute of Advanced Studies Technological Institute of AeronauticsGraduated student of Institute of Science and Technology of São José dos Campos Sao Paulo State University (Unesp)Department of Bioscience and Oral Diagnosis Institute of Science and Technology of São José dos Campos Sao Paulo State University (Unesp), Av. Engenheiro Francisco José Longo, 777Department of Dental Materials and Prosthodontics Institute of Science and Technology of São José dos Campos Sao Paulo State University (Unesp)Graduated student of Institute of Science and Technology of São José dos Campos Sao Paulo State University (Unesp)Universidade Estadual Paulista (Unesp)Technological Institute of Aeronauticsda Cruz Vegian, Mariana Raquel [UNESP]Costa, Bruno César Almeida [UNESP]de Fátima Santana-Melo, Gabriela [UNESP]Godoi, Fernanda Herrera Costa [UNESP]Kaminagakura, Estela [UNESP]Tango, Rubens Nisie [UNESP]do Prado, Renata Falchete [UNESP]de Oliveira, Luciane Dias [UNESP]Federico, Claudio Antoniode Oliveira Marco Avelino, Sarah [UNESP]Neves, Rafael Marques [UNESP]de Vasconcellos, Luana Marotta Reis [UNESP]2019-10-06T16:33:10Z2019-10-06T16:33:10Z2019-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1007/s00784-019-02946-5Clinical Oral Investigations.1436-37711432-6981http://hdl.handle.net/11449/18919810.1007/s00784-019-02946-52-s2.0-8506660192491387166804406030000-0003-0946-1826Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengClinical Oral Investigationsinfo:eu-repo/semantics/openAccess2021-10-23T12:24:32Zoai:repositorio.unesp.br:11449/189198Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:57:51.717240Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Systemic and local effects of radiotherapy: an experimental study on implants placed in rats
title Systemic and local effects of radiotherapy: an experimental study on implants placed in rats
spellingShingle Systemic and local effects of radiotherapy: an experimental study on implants placed in rats
da Cruz Vegian, Mariana Raquel [UNESP]
Bone remodeling
Cytokines
Immunohistochemistry
Radiotherapy
Titanium implants
title_short Systemic and local effects of radiotherapy: an experimental study on implants placed in rats
title_full Systemic and local effects of radiotherapy: an experimental study on implants placed in rats
title_fullStr Systemic and local effects of radiotherapy: an experimental study on implants placed in rats
title_full_unstemmed Systemic and local effects of radiotherapy: an experimental study on implants placed in rats
title_sort Systemic and local effects of radiotherapy: an experimental study on implants placed in rats
author da Cruz Vegian, Mariana Raquel [UNESP]
author_facet da Cruz Vegian, Mariana Raquel [UNESP]
Costa, Bruno César Almeida [UNESP]
de Fátima Santana-Melo, Gabriela [UNESP]
Godoi, Fernanda Herrera Costa [UNESP]
Kaminagakura, Estela [UNESP]
Tango, Rubens Nisie [UNESP]
do Prado, Renata Falchete [UNESP]
de Oliveira, Luciane Dias [UNESP]
Federico, Claudio Antonio
de Oliveira Marco Avelino, Sarah [UNESP]
Neves, Rafael Marques [UNESP]
de Vasconcellos, Luana Marotta Reis [UNESP]
author_role author
author2 Costa, Bruno César Almeida [UNESP]
de Fátima Santana-Melo, Gabriela [UNESP]
Godoi, Fernanda Herrera Costa [UNESP]
Kaminagakura, Estela [UNESP]
Tango, Rubens Nisie [UNESP]
do Prado, Renata Falchete [UNESP]
de Oliveira, Luciane Dias [UNESP]
Federico, Claudio Antonio
de Oliveira Marco Avelino, Sarah [UNESP]
Neves, Rafael Marques [UNESP]
de Vasconcellos, Luana Marotta Reis [UNESP]
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Technological Institute of Aeronautics
dc.contributor.author.fl_str_mv da Cruz Vegian, Mariana Raquel [UNESP]
Costa, Bruno César Almeida [UNESP]
de Fátima Santana-Melo, Gabriela [UNESP]
Godoi, Fernanda Herrera Costa [UNESP]
Kaminagakura, Estela [UNESP]
Tango, Rubens Nisie [UNESP]
do Prado, Renata Falchete [UNESP]
de Oliveira, Luciane Dias [UNESP]
Federico, Claudio Antonio
de Oliveira Marco Avelino, Sarah [UNESP]
Neves, Rafael Marques [UNESP]
de Vasconcellos, Luana Marotta Reis [UNESP]
dc.subject.por.fl_str_mv Bone remodeling
Cytokines
Immunohistochemistry
Radiotherapy
Titanium implants
topic Bone remodeling
Cytokines
Immunohistochemistry
Radiotherapy
Titanium implants
description Objectives: Evaluate the modulating effect of ionizing radiation, blood cytokine levels, and bone remodeling of the interface around the implant to understand the radiation mechanisms which can impair the implants receptor site. Material and methods: Sixty rats were submitted to grade V titanium implants in the femurs and were divided into the following groups: no-irradiation (N-Ir): control group with implant only; early-irradiation (E-Ir): implant + irradiation after 24 h; late-irradiation (L-Ir): implant + irradiation after 4 weeks; and previous-irradiation (P-Ir): irradiation + implant after 4 weeks. The animals in the E-Ir, L-Ir, and P-Ir groups were irradiated in two fractional stages of 15 Gy. At 3 days, 2 weeks, and 7 weeks after the final procedure, five animals were randomly euthanized per group. Serum levels of TNF-ɑ, IL-1β, TGF-β, IL-6, M-CSF, and IL-10 were measured from blood collected prior to euthanasia using the ELISA test. The pieces containing the implants were subjected to immunohistochemical labeling using the tartrate acid resistant to phosphatase, osteocalcin, and caspase-3 markers and mCT. The ANOVA test was used for statistical analysis, and the Tukey multiple comparison test (p < 0.05) was applied. Results: The results indicated that ionizing radiation modifies the production of pro- and anti-inflammatory serum cytokines, the expression of proteins involved in bone remodeling and cellular apoptosis, as well as changes in bone formation. Conclusions: The results suggests that a longer period between radiotherapy and implant placement surgery when irradiation occurs prior to implant installation would allow the recovery and renewal of bone cells and avoid future failures in osseointegration. Clinical relevance: The search for modifications caused by ionizing irradiation in bone tissue can indicate the ideal period for implant placement without affecting the osseointegration process.
publishDate 2019
dc.date.none.fl_str_mv 2019-10-06T16:33:10Z
2019-10-06T16:33:10Z
2019-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1007/s00784-019-02946-5
Clinical Oral Investigations.
1436-3771
1432-6981
http://hdl.handle.net/11449/189198
10.1007/s00784-019-02946-5
2-s2.0-85066601924
9138716680440603
0000-0003-0946-1826
url http://dx.doi.org/10.1007/s00784-019-02946-5
http://hdl.handle.net/11449/189198
identifier_str_mv Clinical Oral Investigations.
1436-3771
1432-6981
10.1007/s00784-019-02946-5
2-s2.0-85066601924
9138716680440603
0000-0003-0946-1826
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Clinical Oral Investigations
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
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