Modulation of macrophage cytokine profiles during solid tumor progression: susceptibility to Candida albicans infection
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1186/1471-2334-9-98 http://hdl.handle.net/11449/8256 |
Resumo: | Background: In order to attain a better understanding of the interactions between opportunist fungi and their hosts, we investigated the cytokine profile associated with the inflammatory response to Candida albicans infection in mice with solid Ehrlich tumors of different degrees.Methods: Groups of eight animals were inoculated intraperitoneally with 5 x 10(6) C. albicans 7, 14 or 21 days after tumor implantation. After 24 or 72 hours, the animals were euthanized and intraperitoneal lavage fluid was collected. Peritoneal macrophages were cultivated and the levels of IFN-gamma, TNF-alpha, IL-12, IL-10 and IL-4 released into the supernatants were measured by ELISA. Kidney, liver and spleen samples were evaluated for fungal dissemination. Tumor-free animals and animals that had only been subjected to C. albicans infection were used as control groups.Results: Our results demonstrated that the mice produced more IFN-gamma and TNF-alpha and less IL-10, and also exhibited fungal clearance, at the beginning of tumor evolution. With the tumor progression, this picture changed: IL-10 production increased and IFN-gamma and TNF-alpha release decreased; furthermore, there was extensive fungal dissemination.Conclusion: Our results indicate that solid tumors can affect the production of macrophage cytokines and, in consequence, affect host resistance to opportunistic infections. |
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Repositório Institucional da UNESP |
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2946 |
spelling |
Modulation of macrophage cytokine profiles during solid tumor progression: susceptibility to Candida albicans infectionBackground: In order to attain a better understanding of the interactions between opportunist fungi and their hosts, we investigated the cytokine profile associated with the inflammatory response to Candida albicans infection in mice with solid Ehrlich tumors of different degrees.Methods: Groups of eight animals were inoculated intraperitoneally with 5 x 10(6) C. albicans 7, 14 or 21 days after tumor implantation. After 24 or 72 hours, the animals were euthanized and intraperitoneal lavage fluid was collected. Peritoneal macrophages were cultivated and the levels of IFN-gamma, TNF-alpha, IL-12, IL-10 and IL-4 released into the supernatants were measured by ELISA. Kidney, liver and spleen samples were evaluated for fungal dissemination. Tumor-free animals and animals that had only been subjected to C. albicans infection were used as control groups.Results: Our results demonstrated that the mice produced more IFN-gamma and TNF-alpha and less IL-10, and also exhibited fungal clearance, at the beginning of tumor evolution. With the tumor progression, this picture changed: IL-10 production increased and IFN-gamma and TNF-alpha release decreased; furthermore, there was extensive fungal dissemination.Conclusion: Our results indicate that solid tumors can affect the production of macrophage cytokines and, in consequence, affect host resistance to opportunistic infections.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)São Paulo State Univ, UNESP, Dept Biol Sci, Expt Immunol Lab,Coll Sci, BR-17047001 Bauru, SP, BrazilSão Paulo State Univ, UNESP, Botucatu Med Sch, BR-18618970 Botucatu, SP, BrazilInst Lauro Souza Lima, BR-17034971 Bauru, SP, BrazilSão Paulo State Univ, UNESP, Dept Biol Sci, Expt Immunol Lab,Coll Sci, BR-17047001 Bauru, SP, BrazilSão Paulo State Univ, UNESP, Botucatu Med Sch, BR-18618970 Botucatu, SP, BrazilBiomed Central Ltd.Universidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Camargo, Marcela Regina de [UNESP]Venturini, James [UNESP]Vilani-Moreno, Fatima R.Arruda, Maria Sueli Parreira de [UNESP]2014-05-20T13:25:54Z2014-05-20T13:25:54Z2009-06-12info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article8application/pdfhttp://dx.doi.org/10.1186/1471-2334-9-98Bmc Infectious Diseases. London: Biomed Central Ltd., v. 9, p. 8, 2009.1471-2334http://hdl.handle.net/11449/825610.1186/1471-2334-9-98WOS:000267750100001WOS000267750100001.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBMC Infectious Diseases2.6201,576info:eu-repo/semantics/openAccess2024-04-23T15:23:49Zoai:repositorio.unesp.br:11449/8256Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:05:09.333122Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Modulation of macrophage cytokine profiles during solid tumor progression: susceptibility to Candida albicans infection |
title |
Modulation of macrophage cytokine profiles during solid tumor progression: susceptibility to Candida albicans infection |
spellingShingle |
Modulation of macrophage cytokine profiles during solid tumor progression: susceptibility to Candida albicans infection Camargo, Marcela Regina de [UNESP] |
title_short |
Modulation of macrophage cytokine profiles during solid tumor progression: susceptibility to Candida albicans infection |
title_full |
Modulation of macrophage cytokine profiles during solid tumor progression: susceptibility to Candida albicans infection |
title_fullStr |
Modulation of macrophage cytokine profiles during solid tumor progression: susceptibility to Candida albicans infection |
title_full_unstemmed |
Modulation of macrophage cytokine profiles during solid tumor progression: susceptibility to Candida albicans infection |
title_sort |
Modulation of macrophage cytokine profiles during solid tumor progression: susceptibility to Candida albicans infection |
author |
Camargo, Marcela Regina de [UNESP] |
author_facet |
Camargo, Marcela Regina de [UNESP] Venturini, James [UNESP] Vilani-Moreno, Fatima R. Arruda, Maria Sueli Parreira de [UNESP] |
author_role |
author |
author2 |
Venturini, James [UNESP] Vilani-Moreno, Fatima R. Arruda, Maria Sueli Parreira de [UNESP] |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Camargo, Marcela Regina de [UNESP] Venturini, James [UNESP] Vilani-Moreno, Fatima R. Arruda, Maria Sueli Parreira de [UNESP] |
description |
Background: In order to attain a better understanding of the interactions between opportunist fungi and their hosts, we investigated the cytokine profile associated with the inflammatory response to Candida albicans infection in mice with solid Ehrlich tumors of different degrees.Methods: Groups of eight animals were inoculated intraperitoneally with 5 x 10(6) C. albicans 7, 14 or 21 days after tumor implantation. After 24 or 72 hours, the animals were euthanized and intraperitoneal lavage fluid was collected. Peritoneal macrophages were cultivated and the levels of IFN-gamma, TNF-alpha, IL-12, IL-10 and IL-4 released into the supernatants were measured by ELISA. Kidney, liver and spleen samples were evaluated for fungal dissemination. Tumor-free animals and animals that had only been subjected to C. albicans infection were used as control groups.Results: Our results demonstrated that the mice produced more IFN-gamma and TNF-alpha and less IL-10, and also exhibited fungal clearance, at the beginning of tumor evolution. With the tumor progression, this picture changed: IL-10 production increased and IFN-gamma and TNF-alpha release decreased; furthermore, there was extensive fungal dissemination.Conclusion: Our results indicate that solid tumors can affect the production of macrophage cytokines and, in consequence, affect host resistance to opportunistic infections. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-06-12 2014-05-20T13:25:54Z 2014-05-20T13:25:54Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1186/1471-2334-9-98 Bmc Infectious Diseases. London: Biomed Central Ltd., v. 9, p. 8, 2009. 1471-2334 http://hdl.handle.net/11449/8256 10.1186/1471-2334-9-98 WOS:000267750100001 WOS000267750100001.pdf |
url |
http://dx.doi.org/10.1186/1471-2334-9-98 http://hdl.handle.net/11449/8256 |
identifier_str_mv |
Bmc Infectious Diseases. London: Biomed Central Ltd., v. 9, p. 8, 2009. 1471-2334 10.1186/1471-2334-9-98 WOS:000267750100001 WOS000267750100001.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
BMC Infectious Diseases 2.620 1,576 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
8 application/pdf |
dc.publisher.none.fl_str_mv |
Biomed Central Ltd. |
publisher.none.fl_str_mv |
Biomed Central Ltd. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129282325610496 |