Follicular lesions with papillary nuclear characteristics: Differences in chromatin detected by computerized image analysis

• Autor(a) principal: Coelho, Bárbara Parente [UNESP]
• Data de Publicação: 2020
• Outros Autores: Valentim, Flávia de Oliveira [UNESP], Miot, Hélio Amante [UNESP], Jaune, Danilo Takeshi Abe [UNESP], Hayashi, Caroline Yuki [UNESP], de Oliveira, Cristiano Claudino [UNESP], Marques, Mariângela de Alencar [UNESP], Tagliarini, José Vicente [UNESP], Castilho, Emanuel Celice [UNESP], Soares, Paula, Mazeto, Gláucia Maria Ferreira da Silva [UNESP]
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.20945/2359-3997000000282; http://hdl.handle.net/11449/206668
• topic: Carcinoma, Papillary, Follicular; Cell nucleus; Histology; Thyroid neoplasms

Objective: Follicular lesions of the thyroid with papillary carcinoma nuclear characteristics are classified as infiltrative follicular variant of papillary thyroid carcinoma-FVPTC (IFVPTC), encapsulated/ well demarcated FVPTC with tumour capsular invasion (IEFVPTC), and the newly described category “non-invasive follicular thyroid neoplasm with papillary-like nuclear features” (NIFTP) formerly known as non-invasive encapsulated FVPTC.This study evaluated whether computerized image analysis can detect nuclear differences between these three tumour subtypes. Materials and methods: Slides with histological material from 15 cases of NIFTP and 33 cases of FVPTC subtypes (22 IEFVPTC, and 11 IFVPTC) were analyzed using the Image J image processing program.Tumour cells were compared for both nuclear morphometry and chromatin textural characteristics. Results: Nuclei from NIFTP and IFVPTC tumours differed in terms of chromatin textural features (grey intensity): mean (92.37 ± 21.01 vs 72.99 ± 14.73, p = 0.02), median (84.93 ± 21.17 vs 65.18 ± 17.08, p = 0.02), standard deviation (47.77 ± 9.55 vs 39.39 ± 7.18; p = 0.02), and coefficient of variation of standard deviation (19.96 ± 4.01 vs 24.75 ± 3.31; p = 0.003). No differences were found in relation to IEFVPTC. Conclusion: Computerized image analysis revealed differences in nuclear texture between NIFTP and IFVPTC, but not for IEFVPTC.