Protective effect of flavonoids from Passiflora edulis Sims on diabetic complications in rats
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1093/jpp/rgab046 http://hdl.handle.net/11449/229693 |
Resumo: | OBJECTIVES: This study was carried out to evaluate the effects of flavonoids present in leaves of Passiflora edulis fruit on complications induced by diabetes in rats. METHODS: The extract of P. edulis leaf was obtained by 70% ethanol maceration. From the dry extract, the fractions were obtained by consecutive liquid-liquid partition with hexane, ethyl acetate and n-butanol. The content of isoorientin of ethyl acetate and n-butanol fractions was determined by ultra-performance liquid chromatography coupled with electrospray and triple quadrupole ionization (TQD) analysis in tandem mass spectrometry (UPLC-ESI-Tq-MS). Only Fr-BuOH was used to treat diabetic or not Wistar rats. Biochemical parameters, platelet aggregation and production of reactive species were evaluated. KEY FINDINGS: The UPLC-ESI-Tq-MS analysis revealed the presence of several flavonoids, among which we identified five possible flavonoids c-heterosides (luteolin-7-O-pyranosyl-3-O-glucoside, apigenin-6-8-di-C-glycoside, apigenin-6-C-arabinoside-8-C-glycoside, isoorientin, isovitexin). The diabetic rats (treated intraperitoneally with alloxan, 150 mg/kg) treated with Fr-BuOH (20 mg/kg/day for 90 days) presented improvement in blood glucose, serum levels of fructosamine, lipid profile and urea. Furthermore, the Fr-BuOH reduced both platelet aggregation and the production of oxidant species in diabetic animals. CONCLUSIONS: These results suggested that flavonoid C-glycosides present in the Fr-BuOH may be beneficial for the diabetic state, preventing complications induced by diabetes. |
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Protective effect of flavonoids from Passiflora edulis Sims on diabetic complications in ratsPassiflora edulisantiplateletdiabetesisoorientinLC-MS quantificationOBJECTIVES: This study was carried out to evaluate the effects of flavonoids present in leaves of Passiflora edulis fruit on complications induced by diabetes in rats. METHODS: The extract of P. edulis leaf was obtained by 70% ethanol maceration. From the dry extract, the fractions were obtained by consecutive liquid-liquid partition with hexane, ethyl acetate and n-butanol. The content of isoorientin of ethyl acetate and n-butanol fractions was determined by ultra-performance liquid chromatography coupled with electrospray and triple quadrupole ionization (TQD) analysis in tandem mass spectrometry (UPLC-ESI-Tq-MS). Only Fr-BuOH was used to treat diabetic or not Wistar rats. Biochemical parameters, platelet aggregation and production of reactive species were evaluated. KEY FINDINGS: The UPLC-ESI-Tq-MS analysis revealed the presence of several flavonoids, among which we identified five possible flavonoids c-heterosides (luteolin-7-O-pyranosyl-3-O-glucoside, apigenin-6-8-di-C-glycoside, apigenin-6-C-arabinoside-8-C-glycoside, isoorientin, isovitexin). The diabetic rats (treated intraperitoneally with alloxan, 150 mg/kg) treated with Fr-BuOH (20 mg/kg/day for 90 days) presented improvement in blood glucose, serum levels of fructosamine, lipid profile and urea. Furthermore, the Fr-BuOH reduced both platelet aggregation and the production of oxidant species in diabetic animals. CONCLUSIONS: These results suggested that flavonoid C-glycosides present in the Fr-BuOH may be beneficial for the diabetic state, preventing complications induced by diabetes.Department of Clinical and Toxicological Analysis Faculty of Pharmaceutical Sciences Federal University of AlfenasDepartment of Chemistry Federal University of MaranhãoInstitute of Biosciences Coastal Campus of São Vicente Universidade Estadual PaulistaDepartment of Botany Institute of Biosciences University of São PauloInstitute of Biosciences Coastal Campus of São Vicente Universidade Estadual PaulistaFederal University of AlfenasFederal University of MaranhãoUniversidade Estadual Paulista (UNESP)Universidade de São Paulo (USP)Salles, Bruno César CorreaLeme, Krissia Carolineda Silva, Marcelo Aparecidoda Rocha, Cláudia QuintinoTangerina, Marcelo M P [UNESP]Vilegas, Wagner [UNESP]Figueiredo, Sônia ADuarte, Stella Maris da SilveiraRodrigues, Maria Ritade Araújo Paula, Fernanda Borges2022-04-29T08:35:08Z2022-04-29T08:35:08Z2021-09-07info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1361-1368http://dx.doi.org/10.1093/jpp/rgab046The Journal of pharmacy and pharmacology, v. 73, n. 10, p. 1361-1368, 2021.2042-7158http://hdl.handle.net/11449/22969310.1093/jpp/rgab0462-s2.0-85116957464Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengThe Journal of pharmacy and pharmacologyinfo:eu-repo/semantics/openAccess2022-04-29T08:35:08Zoai:repositorio.unesp.br:11449/229693Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-04-29T08:35:08Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Protective effect of flavonoids from Passiflora edulis Sims on diabetic complications in rats |
title |
Protective effect of flavonoids from Passiflora edulis Sims on diabetic complications in rats |
spellingShingle |
Protective effect of flavonoids from Passiflora edulis Sims on diabetic complications in rats Salles, Bruno César Correa Passiflora edulis antiplatelet diabetes isoorientin LC-MS quantification |
title_short |
Protective effect of flavonoids from Passiflora edulis Sims on diabetic complications in rats |
title_full |
Protective effect of flavonoids from Passiflora edulis Sims on diabetic complications in rats |
title_fullStr |
Protective effect of flavonoids from Passiflora edulis Sims on diabetic complications in rats |
title_full_unstemmed |
Protective effect of flavonoids from Passiflora edulis Sims on diabetic complications in rats |
title_sort |
Protective effect of flavonoids from Passiflora edulis Sims on diabetic complications in rats |
author |
Salles, Bruno César Correa |
author_facet |
Salles, Bruno César Correa Leme, Krissia Caroline da Silva, Marcelo Aparecido da Rocha, Cláudia Quintino Tangerina, Marcelo M P [UNESP] Vilegas, Wagner [UNESP] Figueiredo, Sônia A Duarte, Stella Maris da Silveira Rodrigues, Maria Rita de Araújo Paula, Fernanda Borges |
author_role |
author |
author2 |
Leme, Krissia Caroline da Silva, Marcelo Aparecido da Rocha, Cláudia Quintino Tangerina, Marcelo M P [UNESP] Vilegas, Wagner [UNESP] Figueiredo, Sônia A Duarte, Stella Maris da Silveira Rodrigues, Maria Rita de Araújo Paula, Fernanda Borges |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Federal University of Alfenas Federal University of Maranhão Universidade Estadual Paulista (UNESP) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Salles, Bruno César Correa Leme, Krissia Caroline da Silva, Marcelo Aparecido da Rocha, Cláudia Quintino Tangerina, Marcelo M P [UNESP] Vilegas, Wagner [UNESP] Figueiredo, Sônia A Duarte, Stella Maris da Silveira Rodrigues, Maria Rita de Araújo Paula, Fernanda Borges |
dc.subject.por.fl_str_mv |
Passiflora edulis antiplatelet diabetes isoorientin LC-MS quantification |
topic |
Passiflora edulis antiplatelet diabetes isoorientin LC-MS quantification |
description |
OBJECTIVES: This study was carried out to evaluate the effects of flavonoids present in leaves of Passiflora edulis fruit on complications induced by diabetes in rats. METHODS: The extract of P. edulis leaf was obtained by 70% ethanol maceration. From the dry extract, the fractions were obtained by consecutive liquid-liquid partition with hexane, ethyl acetate and n-butanol. The content of isoorientin of ethyl acetate and n-butanol fractions was determined by ultra-performance liquid chromatography coupled with electrospray and triple quadrupole ionization (TQD) analysis in tandem mass spectrometry (UPLC-ESI-Tq-MS). Only Fr-BuOH was used to treat diabetic or not Wistar rats. Biochemical parameters, platelet aggregation and production of reactive species were evaluated. KEY FINDINGS: The UPLC-ESI-Tq-MS analysis revealed the presence of several flavonoids, among which we identified five possible flavonoids c-heterosides (luteolin-7-O-pyranosyl-3-O-glucoside, apigenin-6-8-di-C-glycoside, apigenin-6-C-arabinoside-8-C-glycoside, isoorientin, isovitexin). The diabetic rats (treated intraperitoneally with alloxan, 150 mg/kg) treated with Fr-BuOH (20 mg/kg/day for 90 days) presented improvement in blood glucose, serum levels of fructosamine, lipid profile and urea. Furthermore, the Fr-BuOH reduced both platelet aggregation and the production of oxidant species in diabetic animals. CONCLUSIONS: These results suggested that flavonoid C-glycosides present in the Fr-BuOH may be beneficial for the diabetic state, preventing complications induced by diabetes. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-09-07 2022-04-29T08:35:08Z 2022-04-29T08:35:08Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1093/jpp/rgab046 The Journal of pharmacy and pharmacology, v. 73, n. 10, p. 1361-1368, 2021. 2042-7158 http://hdl.handle.net/11449/229693 10.1093/jpp/rgab046 2-s2.0-85116957464 |
url |
http://dx.doi.org/10.1093/jpp/rgab046 http://hdl.handle.net/11449/229693 |
identifier_str_mv |
The Journal of pharmacy and pharmacology, v. 73, n. 10, p. 1361-1368, 2021. 2042-7158 10.1093/jpp/rgab046 2-s2.0-85116957464 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
The Journal of pharmacy and pharmacology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1361-1368 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803649735151583232 |