Impact of perinatal bisphenol A and 17β estradiol exposure: Comparing hormone receptor response
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.ecoenv.2019.109918 http://hdl.handle.net/11449/201374 |
Resumo: | Hormonal regulation controls mammary gland (MG) development. Therefore some hormone-related factors can disrupt the early phases of MGs development, making the gland more susceptible to long term modifications in its response to circulating hormones. Endocrine disruptors, such as bisphenol A (BPA), are able to cause alterations in hormone receptor expression, leading to changes in the cell proliferation index, which may expose the tissue to neoplastic alterations. Thus, we evaluated the variations in hormone receptor expression in the MG of 6-month old Mongolian gerbils exposed to BPA and 17β estradiol during the perinatal period. Receptors for estrogen alpha (ERα), beta (ERβ), progesterone (PGR), prolactin (PRL-R), and co-localization of connexin 43 (Cx43) and ERα in gerbils were analyzed, and serum concentrations of estradiol and progesterone were assessed. No alterations in body, liver, and ovary-uterus complex weights were observed. However, there was an increase in epithelial ERα expression in the 17β estradiol (E2) group and in PGR in the BPA group. Although immunohistochemistry did not show alterations in ERβ expression, western blotting revealed a decrease in this protein in the BPA group. PRL-R was more present in epithelial cells in the vehicle control (VC), E2, and BPA groups in comparison to the intact control group. Cx43 was more frequent in E2 and BPA groups, suggesting a protective response from the gland against possible malignancy. Serum concentration of estradiol reduced in VC, E2, and BPA groups, confirming that alterations also impacts steroid levels. Consequently, perinatal exposure to BPA and the reference endogenous estrogen, 17β estradiol, are able to increase the tendency of endocrine disruption in MG in a long term manner, since repercussions are observed even 6 months after exposure. |
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Impact of perinatal bisphenol A and 17β estradiol exposure: Comparing hormone receptor responseEstrogenEstrogen receptorMammary glandMongolian gerbilProgesterone receptorXenoestrogenHormonal regulation controls mammary gland (MG) development. Therefore some hormone-related factors can disrupt the early phases of MGs development, making the gland more susceptible to long term modifications in its response to circulating hormones. Endocrine disruptors, such as bisphenol A (BPA), are able to cause alterations in hormone receptor expression, leading to changes in the cell proliferation index, which may expose the tissue to neoplastic alterations. Thus, we evaluated the variations in hormone receptor expression in the MG of 6-month old Mongolian gerbils exposed to BPA and 17β estradiol during the perinatal period. Receptors for estrogen alpha (ERα), beta (ERβ), progesterone (PGR), prolactin (PRL-R), and co-localization of connexin 43 (Cx43) and ERα in gerbils were analyzed, and serum concentrations of estradiol and progesterone were assessed. No alterations in body, liver, and ovary-uterus complex weights were observed. However, there was an increase in epithelial ERα expression in the 17β estradiol (E2) group and in PGR in the BPA group. Although immunohistochemistry did not show alterations in ERβ expression, western blotting revealed a decrease in this protein in the BPA group. PRL-R was more present in epithelial cells in the vehicle control (VC), E2, and BPA groups in comparison to the intact control group. Cx43 was more frequent in E2 and BPA groups, suggesting a protective response from the gland against possible malignancy. Serum concentration of estradiol reduced in VC, E2, and BPA groups, confirming that alterations also impacts steroid levels. Consequently, perinatal exposure to BPA and the reference endogenous estrogen, 17β estradiol, are able to increase the tendency of endocrine disruption in MG in a long term manner, since repercussions are observed even 6 months after exposure.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Department of Biology Institute of Biosciences Humanities and Exact Sciences São Paulo State University (UNESP), Rua Cristóvão Colombo 2265, Jardim NazarethFederal University of São João del Rei (UFSJ) Campus Centro Oeste Dona Lindu, Avenida Sebastião Gonçalves Coelho, 400, Bairro ChanadourLaboratory of Gynecology Institute of Experimental and Clinique Research Université Catholique de Louvain (UCL), Avenue Mounier 52, Bte B1.52.02Department of Biology Institute of Biosciences Humanities and Exact Sciences São Paulo State University (UNESP), Rua Cristóvão Colombo 2265, Jardim NazarethCAPES: 001FAPESP: 2015/01548–5FAPESP: 2016/22947–8CNPq: 305840/2015–0Universidade Estadual Paulista (Unesp)Universidade Federal de Sergipe (UFS)Université Catholique de Louvain (UCL)Leonel, Ellen Cristina Rivas [UNESP]Campos, Silvana Gisele PegorinGuerra, Luiz Henrique Alves [UNESP]Bedolo, Carolina Marques [UNESP]Vilamaior, Patrícia Simone Leite [UNESP]Calmon, Marilia Freitas [UNESP]Rahal, Paula [UNESP]Amorim, Christiani AndradeTaboga, Sebastião Roberto [UNESP]2020-12-12T02:30:55Z2020-12-12T02:30:55Z2020-01-30info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.ecoenv.2019.109918Ecotoxicology and Environmental Safety, v. 188.1090-24140147-6513http://hdl.handle.net/11449/20137410.1016/j.ecoenv.2019.1099182-s2.0-8507596218579910823626712120000-0001-5693-6148Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengEcotoxicology and Environmental Safetyinfo:eu-repo/semantics/openAccess2021-10-23T10:11:25Zoai:repositorio.unesp.br:11449/201374Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:38:17.024437Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Impact of perinatal bisphenol A and 17β estradiol exposure: Comparing hormone receptor response |
title |
Impact of perinatal bisphenol A and 17β estradiol exposure: Comparing hormone receptor response |
spellingShingle |
Impact of perinatal bisphenol A and 17β estradiol exposure: Comparing hormone receptor response Leonel, Ellen Cristina Rivas [UNESP] Estrogen Estrogen receptor Mammary gland Mongolian gerbil Progesterone receptor Xenoestrogen |
title_short |
Impact of perinatal bisphenol A and 17β estradiol exposure: Comparing hormone receptor response |
title_full |
Impact of perinatal bisphenol A and 17β estradiol exposure: Comparing hormone receptor response |
title_fullStr |
Impact of perinatal bisphenol A and 17β estradiol exposure: Comparing hormone receptor response |
title_full_unstemmed |
Impact of perinatal bisphenol A and 17β estradiol exposure: Comparing hormone receptor response |
title_sort |
Impact of perinatal bisphenol A and 17β estradiol exposure: Comparing hormone receptor response |
author |
Leonel, Ellen Cristina Rivas [UNESP] |
author_facet |
Leonel, Ellen Cristina Rivas [UNESP] Campos, Silvana Gisele Pegorin Guerra, Luiz Henrique Alves [UNESP] Bedolo, Carolina Marques [UNESP] Vilamaior, Patrícia Simone Leite [UNESP] Calmon, Marilia Freitas [UNESP] Rahal, Paula [UNESP] Amorim, Christiani Andrade Taboga, Sebastião Roberto [UNESP] |
author_role |
author |
author2 |
Campos, Silvana Gisele Pegorin Guerra, Luiz Henrique Alves [UNESP] Bedolo, Carolina Marques [UNESP] Vilamaior, Patrícia Simone Leite [UNESP] Calmon, Marilia Freitas [UNESP] Rahal, Paula [UNESP] Amorim, Christiani Andrade Taboga, Sebastião Roberto [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Federal de Sergipe (UFS) Université Catholique de Louvain (UCL) |
dc.contributor.author.fl_str_mv |
Leonel, Ellen Cristina Rivas [UNESP] Campos, Silvana Gisele Pegorin Guerra, Luiz Henrique Alves [UNESP] Bedolo, Carolina Marques [UNESP] Vilamaior, Patrícia Simone Leite [UNESP] Calmon, Marilia Freitas [UNESP] Rahal, Paula [UNESP] Amorim, Christiani Andrade Taboga, Sebastião Roberto [UNESP] |
dc.subject.por.fl_str_mv |
Estrogen Estrogen receptor Mammary gland Mongolian gerbil Progesterone receptor Xenoestrogen |
topic |
Estrogen Estrogen receptor Mammary gland Mongolian gerbil Progesterone receptor Xenoestrogen |
description |
Hormonal regulation controls mammary gland (MG) development. Therefore some hormone-related factors can disrupt the early phases of MGs development, making the gland more susceptible to long term modifications in its response to circulating hormones. Endocrine disruptors, such as bisphenol A (BPA), are able to cause alterations in hormone receptor expression, leading to changes in the cell proliferation index, which may expose the tissue to neoplastic alterations. Thus, we evaluated the variations in hormone receptor expression in the MG of 6-month old Mongolian gerbils exposed to BPA and 17β estradiol during the perinatal period. Receptors for estrogen alpha (ERα), beta (ERβ), progesterone (PGR), prolactin (PRL-R), and co-localization of connexin 43 (Cx43) and ERα in gerbils were analyzed, and serum concentrations of estradiol and progesterone were assessed. No alterations in body, liver, and ovary-uterus complex weights were observed. However, there was an increase in epithelial ERα expression in the 17β estradiol (E2) group and in PGR in the BPA group. Although immunohistochemistry did not show alterations in ERβ expression, western blotting revealed a decrease in this protein in the BPA group. PRL-R was more present in epithelial cells in the vehicle control (VC), E2, and BPA groups in comparison to the intact control group. Cx43 was more frequent in E2 and BPA groups, suggesting a protective response from the gland against possible malignancy. Serum concentration of estradiol reduced in VC, E2, and BPA groups, confirming that alterations also impacts steroid levels. Consequently, perinatal exposure to BPA and the reference endogenous estrogen, 17β estradiol, are able to increase the tendency of endocrine disruption in MG in a long term manner, since repercussions are observed even 6 months after exposure. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T02:30:55Z 2020-12-12T02:30:55Z 2020-01-30 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.ecoenv.2019.109918 Ecotoxicology and Environmental Safety, v. 188. 1090-2414 0147-6513 http://hdl.handle.net/11449/201374 10.1016/j.ecoenv.2019.109918 2-s2.0-85075962185 7991082362671212 0000-0001-5693-6148 |
url |
http://dx.doi.org/10.1016/j.ecoenv.2019.109918 http://hdl.handle.net/11449/201374 |
identifier_str_mv |
Ecotoxicology and Environmental Safety, v. 188. 1090-2414 0147-6513 10.1016/j.ecoenv.2019.109918 2-s2.0-85075962185 7991082362671212 0000-0001-5693-6148 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Ecotoxicology and Environmental Safety |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129539501457408 |