Efeito da injeção de angiotensina II no Núcleo Parabraquial Lateral (NPBL) na ingestão de água e NaCl hipertônico

• Autor(a) principal: Bearare, Gabriela Patrício [UNESP]
• Data de Publicação: 2011
• Tipo de documento: Trabalho de conclusão de curso
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/155186; http://www.athena.biblioteca.unesp.br/exlibris/bd/capelo/2017-04-25/000880642.pdf
• topic: Angiotensina II; Núcleos parabraquiais; Água; Cloreto de sódio; Angiotensin II

Recent studies showed that losartan, the AT1 angiotensin II antagonist injected into the lateral parabrachial nucleus (LPBN) reduces water and 0.3 M NaCl intake induced by activation of GABAA receptors with muscimol injected in the same site. Angiotensin II (ANG II) is an important peptide involved on sodium intake control. In the present study, we investigated the effects of ANG II injected into the LPBN on water and sodium intake in sodium-depleted rats. Male Wistar rats (280-320 g) with stainless steel cannulas implanted bilaterally into LPBN had access simultaneously to water and 0.3 M NaCl intake. Water and 0.3 M NaCl intakes were tested in rats treated with subcutaneous injections of the diuretic furosemide (FURO - 10 mg/kg) combined with the angiotensin converting enzyme inhibitor captopril (CAP - 5 mg/kg). Bilateral injections of ANG II (50 ng/0.2 L, n=7) into the LPBN did no affect 0.3 M NaCl and water intake induced by FURO+CAP treatment (4.1  1.3 and 8.7  1.7 vs. saline: 5.2  1.2 and 6.6  1.0 mL/150 min, respectively). The pre-treatment with losartan (50 μg/0.2 μL) into the LPBN produced no effects on 0.3 M NaCl and water induced by ANG II (9.4 ± 4.0 and 8.0 ± 2.4 mL/150 min, respectively). Losartan by itself injected into LPBN did not affect water and sodium intake in FURO-CAP treated rats. The results suggest that thirst and sodium apettite is not affected by angiotensinergic mechanisms in the LPBN of sodium-depleted rats