Comparison of different regimens of estradiol benzoate treatments followed by long-acting progesterone to prepare noncycling mares as embryo recipients

Detalhes bibliográficos
Autor(a) principal: Silva, E. S. M. [UNESP]
Data de Publicação: 2016
Outros Autores: Roser, J. F., Gomes, A. R. C. [UNESP], Fritsch, S. C. [UNESP], Pantoja, J. C. F. [UNESP], Oliveira-Filho, J. P. [UNESP], Meira, C. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.theriogenology.2016.05.041
http://hdl.handle.net/11449/161958
Resumo: The present study evaluated the influence of different regimens of estradiol benzoate (EB) treatments followed by a single dose of long-acting progesterone (LA P4) on plasma estrogen and P4 concentrations in noncyclic mares prepared as embryo recipients. Twenty-one anestrous mares were distributed into three groups (n = 7 mares per group), according to the EB dose received (single dose of 2.5 mg, total of 5 mg in decreasing doses, and total of 10 mg in decreasing doses), which was followed by a single administration of 1500 mg of LA P4 in all groups. Mares were reevaluated during the ovulatory phase and seven of them became part of the cyclic nontreated control group. Ultrasonography was performed to monitor endometrial edema, and blood samples were collected to measure estradiol (E2), estrogen conjugate (EC), and P4 by RIA. Maximum uterine edema was achieved 24 hours after administration of EB in all treated groups. Maximum E2 concentrations were observed 24 hours after the first EB injection in treated groups and there were no differences (P > 0.05) among treatments. Maximum EC concentration was observed 24 hours after the single EB injection in the 2.5-mg group, whereas in the 5- and 10-mg groups EC peaks were observed 48 hours after the first EB administration. Maximum P4 concentrations were detected 24 hours after LA P4 injection, although higher P4 concentrations were observed in the group treated with 2.5 mg of EB than in that treated with 10 mg of EB (P < 0.05). Because P4 concentrations were reduced after administration of high doses of EB, we also measured 17 alpha-hydroxyprogesterone (17-OH-P) to test the hypothesis that high concentrations of EB would accelerate the conversion of P4 to 17-OH-P. However, 17-OH-P concentrations paralleled P4 profile in all groups, irrespective of EB doses. In summary, the three EB treatment regimens induced similar E2 peaks, although the observation of EC peaks 24 hours after E2 peaks in the 5- and 10-mg groups indicate that an excess of E2 was given, which was converted into EC to be inactivated. Administration of 10 mg of EB reduced P4 concentrations 24 hours after LA P4 was given. We demonstrated that the mechanism by which this reduction occurred was not by an increase in P4 metabolism to 17 alpha-OH-P. In conclusion, the use of 2.5 mg of EB followed by 1500 mg of LA P4 appears to be a more appropriate regimen to treat noncyclic mares, although additional studies are needed to verify embryo survival with this treatment dose. (C) 2016 Elsevier Inc. All rights reserved.
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spelling Comparison of different regimens of estradiol benzoate treatments followed by long-acting progesterone to prepare noncycling mares as embryo recipientsEquine embryo transferRecipient maresHormonal protocolsEstrogenProgesteroneThe present study evaluated the influence of different regimens of estradiol benzoate (EB) treatments followed by a single dose of long-acting progesterone (LA P4) on plasma estrogen and P4 concentrations in noncyclic mares prepared as embryo recipients. Twenty-one anestrous mares were distributed into three groups (n = 7 mares per group), according to the EB dose received (single dose of 2.5 mg, total of 5 mg in decreasing doses, and total of 10 mg in decreasing doses), which was followed by a single administration of 1500 mg of LA P4 in all groups. Mares were reevaluated during the ovulatory phase and seven of them became part of the cyclic nontreated control group. Ultrasonography was performed to monitor endometrial edema, and blood samples were collected to measure estradiol (E2), estrogen conjugate (EC), and P4 by RIA. Maximum uterine edema was achieved 24 hours after administration of EB in all treated groups. Maximum E2 concentrations were observed 24 hours after the first EB injection in treated groups and there were no differences (P > 0.05) among treatments. Maximum EC concentration was observed 24 hours after the single EB injection in the 2.5-mg group, whereas in the 5- and 10-mg groups EC peaks were observed 48 hours after the first EB administration. Maximum P4 concentrations were detected 24 hours after LA P4 injection, although higher P4 concentrations were observed in the group treated with 2.5 mg of EB than in that treated with 10 mg of EB (P < 0.05). Because P4 concentrations were reduced after administration of high doses of EB, we also measured 17 alpha-hydroxyprogesterone (17-OH-P) to test the hypothesis that high concentrations of EB would accelerate the conversion of P4 to 17-OH-P. However, 17-OH-P concentrations paralleled P4 profile in all groups, irrespective of EB doses. In summary, the three EB treatment regimens induced similar E2 peaks, although the observation of EC peaks 24 hours after E2 peaks in the 5- and 10-mg groups indicate that an excess of E2 was given, which was converted into EC to be inactivated. Administration of 10 mg of EB reduced P4 concentrations 24 hours after LA P4 was given. We demonstrated that the mechanism by which this reduction occurred was not by an increase in P4 metabolism to 17 alpha-OH-P. In conclusion, the use of 2.5 mg of EB followed by 1500 mg of LA P4 appears to be a more appropriate regimen to treat noncyclic mares, although additional studies are needed to verify embryo survival with this treatment dose. (C) 2016 Elsevier Inc. All rights reserved.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Estadual Paulista, Sch Vet Med & Anim Sci, Dept Anim Reprod & Vet Radiol, Botucatu, SP, BrazilUniv Calif Davis, Dept Anim Sci, Davis, CA 95616 USAUniv Estadual Paulista, Sch Vet Med & Anim Sci, Dept Vet Hyg & Publ Hlth, Botucatu, SP, BrazilUniv Estadual Paulista, Sch Vet Med & Anim Sci, Dept Vet Clin Sci, Botucatu, SP, BrazilUniv Estadual Paulista, Sch Vet Med & Anim Sci, Dept Anim Reprod & Vet Radiol, Botucatu, SP, BrazilUniv Estadual Paulista, Sch Vet Med & Anim Sci, Dept Vet Hyg & Publ Hlth, Botucatu, SP, BrazilUniv Estadual Paulista, Sch Vet Med & Anim Sci, Dept Vet Clin Sci, Botucatu, SP, BrazilFAPESP: 2014/08207-6Elsevier B.V.Universidade Estadual Paulista (Unesp)Univ Calif DavisSilva, E. S. M. [UNESP]Roser, J. F.Gomes, A. R. C. [UNESP]Fritsch, S. C. [UNESP]Pantoja, J. C. F. [UNESP]Oliveira-Filho, J. P. [UNESP]Meira, C. [UNESP]2018-11-26T17:06:20Z2018-11-26T17:06:20Z2016-10-15info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1749-1756application/pdfhttp://dx.doi.org/10.1016/j.theriogenology.2016.05.041Theriogenology. New York: Elsevier Science Inc, v. 86, n. 7, p. 1749-1756, 2016.0093-691Xhttp://hdl.handle.net/11449/16195810.1016/j.theriogenology.2016.05.041WOS:000383935500013WOS000383935500013.pdf00429054157111990000-0002-2245-800XWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengTheriogenologyinfo:eu-repo/semantics/openAccess2024-09-09T14:00:29Zoai:repositorio.unesp.br:11449/161958Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-09T14:00:29Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Comparison of different regimens of estradiol benzoate treatments followed by long-acting progesterone to prepare noncycling mares as embryo recipients
title Comparison of different regimens of estradiol benzoate treatments followed by long-acting progesterone to prepare noncycling mares as embryo recipients
spellingShingle Comparison of different regimens of estradiol benzoate treatments followed by long-acting progesterone to prepare noncycling mares as embryo recipients
Silva, E. S. M. [UNESP]
Equine embryo transfer
Recipient mares
Hormonal protocols
Estrogen
Progesterone
title_short Comparison of different regimens of estradiol benzoate treatments followed by long-acting progesterone to prepare noncycling mares as embryo recipients
title_full Comparison of different regimens of estradiol benzoate treatments followed by long-acting progesterone to prepare noncycling mares as embryo recipients
title_fullStr Comparison of different regimens of estradiol benzoate treatments followed by long-acting progesterone to prepare noncycling mares as embryo recipients
title_full_unstemmed Comparison of different regimens of estradiol benzoate treatments followed by long-acting progesterone to prepare noncycling mares as embryo recipients
title_sort Comparison of different regimens of estradiol benzoate treatments followed by long-acting progesterone to prepare noncycling mares as embryo recipients
author Silva, E. S. M. [UNESP]
author_facet Silva, E. S. M. [UNESP]
Roser, J. F.
Gomes, A. R. C. [UNESP]
Fritsch, S. C. [UNESP]
Pantoja, J. C. F. [UNESP]
Oliveira-Filho, J. P. [UNESP]
Meira, C. [UNESP]
author_role author
author2 Roser, J. F.
Gomes, A. R. C. [UNESP]
Fritsch, S. C. [UNESP]
Pantoja, J. C. F. [UNESP]
Oliveira-Filho, J. P. [UNESP]
Meira, C. [UNESP]
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Univ Calif Davis
dc.contributor.author.fl_str_mv Silva, E. S. M. [UNESP]
Roser, J. F.
Gomes, A. R. C. [UNESP]
Fritsch, S. C. [UNESP]
Pantoja, J. C. F. [UNESP]
Oliveira-Filho, J. P. [UNESP]
Meira, C. [UNESP]
dc.subject.por.fl_str_mv Equine embryo transfer
Recipient mares
Hormonal protocols
Estrogen
Progesterone
topic Equine embryo transfer
Recipient mares
Hormonal protocols
Estrogen
Progesterone
description The present study evaluated the influence of different regimens of estradiol benzoate (EB) treatments followed by a single dose of long-acting progesterone (LA P4) on plasma estrogen and P4 concentrations in noncyclic mares prepared as embryo recipients. Twenty-one anestrous mares were distributed into three groups (n = 7 mares per group), according to the EB dose received (single dose of 2.5 mg, total of 5 mg in decreasing doses, and total of 10 mg in decreasing doses), which was followed by a single administration of 1500 mg of LA P4 in all groups. Mares were reevaluated during the ovulatory phase and seven of them became part of the cyclic nontreated control group. Ultrasonography was performed to monitor endometrial edema, and blood samples were collected to measure estradiol (E2), estrogen conjugate (EC), and P4 by RIA. Maximum uterine edema was achieved 24 hours after administration of EB in all treated groups. Maximum E2 concentrations were observed 24 hours after the first EB injection in treated groups and there were no differences (P > 0.05) among treatments. Maximum EC concentration was observed 24 hours after the single EB injection in the 2.5-mg group, whereas in the 5- and 10-mg groups EC peaks were observed 48 hours after the first EB administration. Maximum P4 concentrations were detected 24 hours after LA P4 injection, although higher P4 concentrations were observed in the group treated with 2.5 mg of EB than in that treated with 10 mg of EB (P < 0.05). Because P4 concentrations were reduced after administration of high doses of EB, we also measured 17 alpha-hydroxyprogesterone (17-OH-P) to test the hypothesis that high concentrations of EB would accelerate the conversion of P4 to 17-OH-P. However, 17-OH-P concentrations paralleled P4 profile in all groups, irrespective of EB doses. In summary, the three EB treatment regimens induced similar E2 peaks, although the observation of EC peaks 24 hours after E2 peaks in the 5- and 10-mg groups indicate that an excess of E2 was given, which was converted into EC to be inactivated. Administration of 10 mg of EB reduced P4 concentrations 24 hours after LA P4 was given. We demonstrated that the mechanism by which this reduction occurred was not by an increase in P4 metabolism to 17 alpha-OH-P. In conclusion, the use of 2.5 mg of EB followed by 1500 mg of LA P4 appears to be a more appropriate regimen to treat noncyclic mares, although additional studies are needed to verify embryo survival with this treatment dose. (C) 2016 Elsevier Inc. All rights reserved.
publishDate 2016
dc.date.none.fl_str_mv 2016-10-15
2018-11-26T17:06:20Z
2018-11-26T17:06:20Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.theriogenology.2016.05.041
Theriogenology. New York: Elsevier Science Inc, v. 86, n. 7, p. 1749-1756, 2016.
0093-691X
http://hdl.handle.net/11449/161958
10.1016/j.theriogenology.2016.05.041
WOS:000383935500013
WOS000383935500013.pdf
0042905415711199
0000-0002-2245-800X
url http://dx.doi.org/10.1016/j.theriogenology.2016.05.041
http://hdl.handle.net/11449/161958
identifier_str_mv Theriogenology. New York: Elsevier Science Inc, v. 86, n. 7, p. 1749-1756, 2016.
0093-691X
10.1016/j.theriogenology.2016.05.041
WOS:000383935500013
WOS000383935500013.pdf
0042905415711199
0000-0002-2245-800X
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Theriogenology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 1749-1756
application/pdf
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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