Cost-effective composite electrode for the fast voltammetric screening and determination of riboflavin (B2) and pyridoxine (B6) in pharmaceuticals
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://www.uff.br/RVQ/index.php/rvq/article/view/384 http://hdl.handle.net/11449/76628 |
Resumo: | Different solid composites made by mechanical dispersions of graphite particles into heated paraffin (from 65 to 80% graphite, in mass) were prepared and assessed in order to optimize their use in electrochemical and electroanalytical procedures for bioanalysis. Besides these, composites were also evaluated by thermoanalytical techniques aiming to study their conservation and long-term stability (over eight months without special care), among others. Best results were found at 80% m/m graphite in paraffin. Such electrode combines low-cost, stability, sensitivity, ease of maintenance and clearance, besides the possibilities of manufacture in many different forms and shapes (with or without modifications) and applicability in a wide range of pH. Electrochemical studies by different voltammetric techniques involving vitamins from complex B (riboflavin and pyridoxine) leaded to a better understanding about their electrooxidative processes onto carbon-composite electrodes, specially regarding reversibility and pH-dependence. Data were also acquired and optimized with analytical purposes, being square-wave voltammetry in pH 4.2 chosen by its many advantages. Good linearity between peak responses as function of concentration were reached from 5 to 43 μmol L-1 for riboflavin (peak at -0.257 V) and up to 8.5 × 10-4 mol L -1 for pyridoxine (peak at +1.04 V), best studied conditions; limits of detection (at an S/N of 3) for both analites showed to be circa 1.0 mol L-1. Different commercial samples were analyzed for riboflavin (EMS® complex B syrup) and pyridoxine (Citoneurin 5000 Merck® ampoules) providing 96.6% and 98.7% recoveries, respectively. |
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Cost-effective composite electrode for the fast voltammetric screening and determination of riboflavin (B2) and pyridoxine (B6) in pharmaceuticalsComposite electrodesPyridoxineRiboflavinVoltammetric analysisDifferent solid composites made by mechanical dispersions of graphite particles into heated paraffin (from 65 to 80% graphite, in mass) were prepared and assessed in order to optimize their use in electrochemical and electroanalytical procedures for bioanalysis. Besides these, composites were also evaluated by thermoanalytical techniques aiming to study their conservation and long-term stability (over eight months without special care), among others. Best results were found at 80% m/m graphite in paraffin. Such electrode combines low-cost, stability, sensitivity, ease of maintenance and clearance, besides the possibilities of manufacture in many different forms and shapes (with or without modifications) and applicability in a wide range of pH. Electrochemical studies by different voltammetric techniques involving vitamins from complex B (riboflavin and pyridoxine) leaded to a better understanding about their electrooxidative processes onto carbon-composite electrodes, specially regarding reversibility and pH-dependence. Data were also acquired and optimized with analytical purposes, being square-wave voltammetry in pH 4.2 chosen by its many advantages. Good linearity between peak responses as function of concentration were reached from 5 to 43 μmol L-1 for riboflavin (peak at -0.257 V) and up to 8.5 × 10-4 mol L -1 for pyridoxine (peak at +1.04 V), best studied conditions; limits of detection (at an S/N of 3) for both analites showed to be circa 1.0 mol L-1. Different commercial samples were analyzed for riboflavin (EMS® complex B syrup) and pyridoxine (Citoneurin 5000 Merck® ampoules) providing 96.6% and 98.7% recoveries, respectively.Universidade Federal Fluminense Instituto de Química Departamento de Química Analítica, Rua Outeiro São João Batista s/n, CEP 2402-150, Niterói-RJUniversidade Estadual Paulista Departamento de Física e Química Faculdade de Engenharia de Guaratinguetá, Avenida Doutor Ariberto Pereira da Cunha, 333, CEP 12516-410, Guaratinguetá-SPUniversidade Estadual Paulista Departamento de Física e Química Faculdade de Engenharia de Guaratinguetá, Avenida Doutor Ariberto Pereira da Cunha, 333, CEP 12516-410, Guaratinguetá-SPUniversidade Federal Fluminense (UFF)Universidade Estadual Paulista (Unesp)Dos Santos, Thiago A.D.Barreto, Lucas N.Ritta, Almir Guilherme S. L.De Meneses, Wanessa S.Nunes, Ronaldo S. [UNESP]Semaan, Felipe S.2014-05-27T11:30:44Z2014-05-27T11:30:44Z2013-09-23info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article548-562application/pdfhttp://www.uff.br/RVQ/index.php/rvq/article/view/384Revista Virtual de Quimica, v. 5, n. 4, p. 548-562, 2013.1984-6835http://hdl.handle.net/11449/766282-s2.0-848842482872-s2.0-84884248287.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista Virtual de Quimica0,127info:eu-repo/semantics/openAccess2024-07-01T20:52:27Zoai:repositorio.unesp.br:11449/76628Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:28:14.700439Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Cost-effective composite electrode for the fast voltammetric screening and determination of riboflavin (B2) and pyridoxine (B6) in pharmaceuticals |
title |
Cost-effective composite electrode for the fast voltammetric screening and determination of riboflavin (B2) and pyridoxine (B6) in pharmaceuticals |
spellingShingle |
Cost-effective composite electrode for the fast voltammetric screening and determination of riboflavin (B2) and pyridoxine (B6) in pharmaceuticals Dos Santos, Thiago A.D. Composite electrodes Pyridoxine Riboflavin Voltammetric analysis |
title_short |
Cost-effective composite electrode for the fast voltammetric screening and determination of riboflavin (B2) and pyridoxine (B6) in pharmaceuticals |
title_full |
Cost-effective composite electrode for the fast voltammetric screening and determination of riboflavin (B2) and pyridoxine (B6) in pharmaceuticals |
title_fullStr |
Cost-effective composite electrode for the fast voltammetric screening and determination of riboflavin (B2) and pyridoxine (B6) in pharmaceuticals |
title_full_unstemmed |
Cost-effective composite electrode for the fast voltammetric screening and determination of riboflavin (B2) and pyridoxine (B6) in pharmaceuticals |
title_sort |
Cost-effective composite electrode for the fast voltammetric screening and determination of riboflavin (B2) and pyridoxine (B6) in pharmaceuticals |
author |
Dos Santos, Thiago A.D. |
author_facet |
Dos Santos, Thiago A.D. Barreto, Lucas N. Ritta, Almir Guilherme S. L. De Meneses, Wanessa S. Nunes, Ronaldo S. [UNESP] Semaan, Felipe S. |
author_role |
author |
author2 |
Barreto, Lucas N. Ritta, Almir Guilherme S. L. De Meneses, Wanessa S. Nunes, Ronaldo S. [UNESP] Semaan, Felipe S. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal Fluminense (UFF) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Dos Santos, Thiago A.D. Barreto, Lucas N. Ritta, Almir Guilherme S. L. De Meneses, Wanessa S. Nunes, Ronaldo S. [UNESP] Semaan, Felipe S. |
dc.subject.por.fl_str_mv |
Composite electrodes Pyridoxine Riboflavin Voltammetric analysis |
topic |
Composite electrodes Pyridoxine Riboflavin Voltammetric analysis |
description |
Different solid composites made by mechanical dispersions of graphite particles into heated paraffin (from 65 to 80% graphite, in mass) were prepared and assessed in order to optimize their use in electrochemical and electroanalytical procedures for bioanalysis. Besides these, composites were also evaluated by thermoanalytical techniques aiming to study their conservation and long-term stability (over eight months without special care), among others. Best results were found at 80% m/m graphite in paraffin. Such electrode combines low-cost, stability, sensitivity, ease of maintenance and clearance, besides the possibilities of manufacture in many different forms and shapes (with or without modifications) and applicability in a wide range of pH. Electrochemical studies by different voltammetric techniques involving vitamins from complex B (riboflavin and pyridoxine) leaded to a better understanding about their electrooxidative processes onto carbon-composite electrodes, specially regarding reversibility and pH-dependence. Data were also acquired and optimized with analytical purposes, being square-wave voltammetry in pH 4.2 chosen by its many advantages. Good linearity between peak responses as function of concentration were reached from 5 to 43 μmol L-1 for riboflavin (peak at -0.257 V) and up to 8.5 × 10-4 mol L -1 for pyridoxine (peak at +1.04 V), best studied conditions; limits of detection (at an S/N of 3) for both analites showed to be circa 1.0 mol L-1. Different commercial samples were analyzed for riboflavin (EMS® complex B syrup) and pyridoxine (Citoneurin 5000 Merck® ampoules) providing 96.6% and 98.7% recoveries, respectively. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-09-23 2014-05-27T11:30:44Z 2014-05-27T11:30:44Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.uff.br/RVQ/index.php/rvq/article/view/384 Revista Virtual de Quimica, v. 5, n. 4, p. 548-562, 2013. 1984-6835 http://hdl.handle.net/11449/76628 2-s2.0-84884248287 2-s2.0-84884248287.pdf |
url |
http://www.uff.br/RVQ/index.php/rvq/article/view/384 http://hdl.handle.net/11449/76628 |
identifier_str_mv |
Revista Virtual de Quimica, v. 5, n. 4, p. 548-562, 2013. 1984-6835 2-s2.0-84884248287 2-s2.0-84884248287.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Revista Virtual de Quimica 0,127 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
548-562 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129206514614272 |