Nephrocalcinosis induced by hyperoxaluria in rats

Detalhes bibliográficos
Autor(a) principal: Cunha, Natália Baraldi [UNESP]
Data de Publicação: 2013
Outros Autores: Kawano, Paulo Roberto [UNESP], Padovani, Carlos Roberto [UNESP], Lima, Flávio de Oliveira [UNESP], Bernardes, Suene, Magalhães, Eloá Siqueira [UNESP], Amaro, Carmen Regina Petean [UNESP], Amaro, João Luiz [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S0102-86502013000700004
http://hdl.handle.net/11449/75842
Resumo: Purpose: To investigate nephrocalcinosis due to hyperoxaluria induced by two different inducing agents in rats. Methods: Forty Sprague-Dawley male rats were randomly distributed into four groups: Group1 (Clinical control, n = 10); Group 2 (0.5% Ethylene Glycol + Vitamin D3, n = 10); Group 3 (1.25% Ethylene Glycol, n = 10); and Group 4 (5%Hydroxy L-proline, n = 10). Five animals from each group were euthanized after one week of follow-up (M1 Moment) and the remaining, after four weeks (M2 Moment). All animals underwent 24h urine dosages of calcium, oxalate, uric acid, citrate and serum creatinine. Histology and histomorphometric analyses were performed using Image J program in the hematoxylin-eosin stains. Calcium deposits in the renal parenchyma were quantified by PIXE technique (Proton Induced X-Ray Emission). Results: 24h urinary parameters did not show any significant variations after 28 days of experiment except by hyperoxaluria that was significantly higher in Group 3. Histomorphometric analyses showed a significantly higher nephrocalcinosis in Group 2 (p<0.01). The calcium deposits in the renal parenchyma were 10 and 100 times higher in Group 2 in comparison to other groups in the M1 and M2 moments, respectively. Conclusion: The Group 2 (vitamin D3+Ethylene Glycol 0.5%) was the best model to induce nephrocalcinosis in rats after 28 days.
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spelling Nephrocalcinosis induced by hyperoxaluria in ratsHyperoxaluriaLithiasisNephrocalcinosisRatscalciumcalcium oxalatecitric acidcreatinineoxalic aciduric acidanimal experimentanimal modelanimal tissuecontrolled studyhistologyhyperoxaluriakidney calcificationkidney parenchymamalemorphometricsnonhumanraturine pHurine volumePurpose: To investigate nephrocalcinosis due to hyperoxaluria induced by two different inducing agents in rats. Methods: Forty Sprague-Dawley male rats were randomly distributed into four groups: Group1 (Clinical control, n = 10); Group 2 (0.5% Ethylene Glycol + Vitamin D3, n = 10); Group 3 (1.25% Ethylene Glycol, n = 10); and Group 4 (5%Hydroxy L-proline, n = 10). Five animals from each group were euthanized after one week of follow-up (M1 Moment) and the remaining, after four weeks (M2 Moment). All animals underwent 24h urine dosages of calcium, oxalate, uric acid, citrate and serum creatinine. Histology and histomorphometric analyses were performed using Image J program in the hematoxylin-eosin stains. Calcium deposits in the renal parenchyma were quantified by PIXE technique (Proton Induced X-Ray Emission). Results: 24h urinary parameters did not show any significant variations after 28 days of experiment except by hyperoxaluria that was significantly higher in Group 3. Histomorphometric analyses showed a significantly higher nephrocalcinosis in Group 2 (p<0.01). The calcium deposits in the renal parenchyma were 10 and 100 times higher in Group 2 in comparison to other groups in the M1 and M2 moments, respectively. Conclusion: The Group 2 (vitamin D3+Ethylene Glycol 0.5%) was the best model to induce nephrocalcinosis in rats after 28 days.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Botucatu School of Medicine São Paulo State University UNESP, Botucatu-SPDepartment of Urology Botucatu School of Medicine São Paulo State University, UNESP, Botucatu-SPDepartment of Biostatistics Botucatu Institute of Biosciences São Paulo State University, UNESP, Botucatu-SPDepartment of Pathology Botucatu School of Medicine São Paulo State University, UNESP, Botucatu-SPInstitute of Physics University of Sao Paulo (USP)IC FAPESP Botucatu Institute of Biosciences São Paulo State University, UNESP, Botucatu-SPBotucatu School of Medicine São Paulo State University UNESP, Botucatu-SPDepartment of Urology Botucatu School of Medicine São Paulo State University, UNESP, Botucatu-SPDepartment of Biostatistics Botucatu Institute of Biosciences São Paulo State University, UNESP, Botucatu-SPDepartment of Pathology Botucatu School of Medicine São Paulo State University, UNESP, Botucatu-SPIC FAPESP Botucatu Institute of Biosciences São Paulo State University, UNESP, Botucatu-SPFAPESP: 11/116990Universidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Cunha, Natália Baraldi [UNESP]Kawano, Paulo Roberto [UNESP]Padovani, Carlos Roberto [UNESP]Lima, Flávio de Oliveira [UNESP]Bernardes, SueneMagalhães, Eloá Siqueira [UNESP]Amaro, Carmen Regina Petean [UNESP]Amaro, João Luiz [UNESP]2014-05-27T11:29:53Z2014-05-27T11:29:53Z2013-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article496-501application/pdfhttp://dx.doi.org/10.1590/S0102-86502013000700004Acta Cirurgica Brasileira, v. 28, n. 7, p. 496-501, 2013.0102-86501678-2674http://hdl.handle.net/11449/7584210.1590/S0102-86502013000700004S0102-86502013000700004WOS:0003224070000042-s2.0-848803934312-s2.0-84880393431.pdf2443296326760741872789708052228999898578543556920000-0001-8411-5822Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengActa Cirúrgica Brasileira0.9330,395info:eu-repo/semantics/openAccess2024-09-03T14:30:00Zoai:repositorio.unesp.br:11449/75842Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-03T14:30Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Nephrocalcinosis induced by hyperoxaluria in rats
title Nephrocalcinosis induced by hyperoxaluria in rats
spellingShingle Nephrocalcinosis induced by hyperoxaluria in rats
Cunha, Natália Baraldi [UNESP]
Hyperoxaluria
Lithiasis
Nephrocalcinosis
Rats
calcium
calcium oxalate
citric acid
creatinine
oxalic acid
uric acid
animal experiment
animal model
animal tissue
controlled study
histology
hyperoxaluria
kidney calcification
kidney parenchyma
male
morphometrics
nonhuman
rat
urine pH
urine volume
title_short Nephrocalcinosis induced by hyperoxaluria in rats
title_full Nephrocalcinosis induced by hyperoxaluria in rats
title_fullStr Nephrocalcinosis induced by hyperoxaluria in rats
title_full_unstemmed Nephrocalcinosis induced by hyperoxaluria in rats
title_sort Nephrocalcinosis induced by hyperoxaluria in rats
author Cunha, Natália Baraldi [UNESP]
author_facet Cunha, Natália Baraldi [UNESP]
Kawano, Paulo Roberto [UNESP]
Padovani, Carlos Roberto [UNESP]
Lima, Flávio de Oliveira [UNESP]
Bernardes, Suene
Magalhães, Eloá Siqueira [UNESP]
Amaro, Carmen Regina Petean [UNESP]
Amaro, João Luiz [UNESP]
author_role author
author2 Kawano, Paulo Roberto [UNESP]
Padovani, Carlos Roberto [UNESP]
Lima, Flávio de Oliveira [UNESP]
Bernardes, Suene
Magalhães, Eloá Siqueira [UNESP]
Amaro, Carmen Regina Petean [UNESP]
Amaro, João Luiz [UNESP]
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv Cunha, Natália Baraldi [UNESP]
Kawano, Paulo Roberto [UNESP]
Padovani, Carlos Roberto [UNESP]
Lima, Flávio de Oliveira [UNESP]
Bernardes, Suene
Magalhães, Eloá Siqueira [UNESP]
Amaro, Carmen Regina Petean [UNESP]
Amaro, João Luiz [UNESP]
dc.subject.por.fl_str_mv Hyperoxaluria
Lithiasis
Nephrocalcinosis
Rats
calcium
calcium oxalate
citric acid
creatinine
oxalic acid
uric acid
animal experiment
animal model
animal tissue
controlled study
histology
hyperoxaluria
kidney calcification
kidney parenchyma
male
morphometrics
nonhuman
rat
urine pH
urine volume
topic Hyperoxaluria
Lithiasis
Nephrocalcinosis
Rats
calcium
calcium oxalate
citric acid
creatinine
oxalic acid
uric acid
animal experiment
animal model
animal tissue
controlled study
histology
hyperoxaluria
kidney calcification
kidney parenchyma
male
morphometrics
nonhuman
rat
urine pH
urine volume
description Purpose: To investigate nephrocalcinosis due to hyperoxaluria induced by two different inducing agents in rats. Methods: Forty Sprague-Dawley male rats were randomly distributed into four groups: Group1 (Clinical control, n = 10); Group 2 (0.5% Ethylene Glycol + Vitamin D3, n = 10); Group 3 (1.25% Ethylene Glycol, n = 10); and Group 4 (5%Hydroxy L-proline, n = 10). Five animals from each group were euthanized after one week of follow-up (M1 Moment) and the remaining, after four weeks (M2 Moment). All animals underwent 24h urine dosages of calcium, oxalate, uric acid, citrate and serum creatinine. Histology and histomorphometric analyses were performed using Image J program in the hematoxylin-eosin stains. Calcium deposits in the renal parenchyma were quantified by PIXE technique (Proton Induced X-Ray Emission). Results: 24h urinary parameters did not show any significant variations after 28 days of experiment except by hyperoxaluria that was significantly higher in Group 3. Histomorphometric analyses showed a significantly higher nephrocalcinosis in Group 2 (p<0.01). The calcium deposits in the renal parenchyma were 10 and 100 times higher in Group 2 in comparison to other groups in the M1 and M2 moments, respectively. Conclusion: The Group 2 (vitamin D3+Ethylene Glycol 0.5%) was the best model to induce nephrocalcinosis in rats after 28 days.
publishDate 2013
dc.date.none.fl_str_mv 2013-07-01
2014-05-27T11:29:53Z
2014-05-27T11:29:53Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S0102-86502013000700004
Acta Cirurgica Brasileira, v. 28, n. 7, p. 496-501, 2013.
0102-8650
1678-2674
http://hdl.handle.net/11449/75842
10.1590/S0102-86502013000700004
S0102-86502013000700004
WOS:000322407000004
2-s2.0-84880393431
2-s2.0-84880393431.pdf
2443296326760741
8727897080522289
9989857854355692
0000-0001-8411-5822
url http://dx.doi.org/10.1590/S0102-86502013000700004
http://hdl.handle.net/11449/75842
identifier_str_mv Acta Cirurgica Brasileira, v. 28, n. 7, p. 496-501, 2013.
0102-8650
1678-2674
10.1590/S0102-86502013000700004
S0102-86502013000700004
WOS:000322407000004
2-s2.0-84880393431
2-s2.0-84880393431.pdf
2443296326760741
8727897080522289
9989857854355692
0000-0001-8411-5822
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Acta Cirúrgica Brasileira
0.933
0,395
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 496-501
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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