Nephrocalcinosis induced by hyperoxaluria in rats
Autor(a) principal: | |
---|---|
Data de Publicação: | 2013 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S0102-86502013000700004 http://hdl.handle.net/11449/75842 |
Resumo: | Purpose: To investigate nephrocalcinosis due to hyperoxaluria induced by two different inducing agents in rats. Methods: Forty Sprague-Dawley male rats were randomly distributed into four groups: Group1 (Clinical control, n = 10); Group 2 (0.5% Ethylene Glycol + Vitamin D3, n = 10); Group 3 (1.25% Ethylene Glycol, n = 10); and Group 4 (5%Hydroxy L-proline, n = 10). Five animals from each group were euthanized after one week of follow-up (M1 Moment) and the remaining, after four weeks (M2 Moment). All animals underwent 24h urine dosages of calcium, oxalate, uric acid, citrate and serum creatinine. Histology and histomorphometric analyses were performed using Image J program in the hematoxylin-eosin stains. Calcium deposits in the renal parenchyma were quantified by PIXE technique (Proton Induced X-Ray Emission). Results: 24h urinary parameters did not show any significant variations after 28 days of experiment except by hyperoxaluria that was significantly higher in Group 3. Histomorphometric analyses showed a significantly higher nephrocalcinosis in Group 2 (p<0.01). The calcium deposits in the renal parenchyma were 10 and 100 times higher in Group 2 in comparison to other groups in the M1 and M2 moments, respectively. Conclusion: The Group 2 (vitamin D3+Ethylene Glycol 0.5%) was the best model to induce nephrocalcinosis in rats after 28 days. |
id |
UNSP_c776a6148142332183a3ee1ee8e29af4 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/75842 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Nephrocalcinosis induced by hyperoxaluria in ratsHyperoxaluriaLithiasisNephrocalcinosisRatscalciumcalcium oxalatecitric acidcreatinineoxalic aciduric acidanimal experimentanimal modelanimal tissuecontrolled studyhistologyhyperoxaluriakidney calcificationkidney parenchymamalemorphometricsnonhumanraturine pHurine volumePurpose: To investigate nephrocalcinosis due to hyperoxaluria induced by two different inducing agents in rats. Methods: Forty Sprague-Dawley male rats were randomly distributed into four groups: Group1 (Clinical control, n = 10); Group 2 (0.5% Ethylene Glycol + Vitamin D3, n = 10); Group 3 (1.25% Ethylene Glycol, n = 10); and Group 4 (5%Hydroxy L-proline, n = 10). Five animals from each group were euthanized after one week of follow-up (M1 Moment) and the remaining, after four weeks (M2 Moment). All animals underwent 24h urine dosages of calcium, oxalate, uric acid, citrate and serum creatinine. Histology and histomorphometric analyses were performed using Image J program in the hematoxylin-eosin stains. Calcium deposits in the renal parenchyma were quantified by PIXE technique (Proton Induced X-Ray Emission). Results: 24h urinary parameters did not show any significant variations after 28 days of experiment except by hyperoxaluria that was significantly higher in Group 3. Histomorphometric analyses showed a significantly higher nephrocalcinosis in Group 2 (p<0.01). The calcium deposits in the renal parenchyma were 10 and 100 times higher in Group 2 in comparison to other groups in the M1 and M2 moments, respectively. Conclusion: The Group 2 (vitamin D3+Ethylene Glycol 0.5%) was the best model to induce nephrocalcinosis in rats after 28 days.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Botucatu School of Medicine São Paulo State University UNESP, Botucatu-SPDepartment of Urology Botucatu School of Medicine São Paulo State University, UNESP, Botucatu-SPDepartment of Biostatistics Botucatu Institute of Biosciences São Paulo State University, UNESP, Botucatu-SPDepartment of Pathology Botucatu School of Medicine São Paulo State University, UNESP, Botucatu-SPInstitute of Physics University of Sao Paulo (USP)IC FAPESP Botucatu Institute of Biosciences São Paulo State University, UNESP, Botucatu-SPBotucatu School of Medicine São Paulo State University UNESP, Botucatu-SPDepartment of Urology Botucatu School of Medicine São Paulo State University, UNESP, Botucatu-SPDepartment of Biostatistics Botucatu Institute of Biosciences São Paulo State University, UNESP, Botucatu-SPDepartment of Pathology Botucatu School of Medicine São Paulo State University, UNESP, Botucatu-SPIC FAPESP Botucatu Institute of Biosciences São Paulo State University, UNESP, Botucatu-SPFAPESP: 11/116990Universidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Cunha, Natália Baraldi [UNESP]Kawano, Paulo Roberto [UNESP]Padovani, Carlos Roberto [UNESP]Lima, Flávio de Oliveira [UNESP]Bernardes, SueneMagalhães, Eloá Siqueira [UNESP]Amaro, Carmen Regina Petean [UNESP]Amaro, João Luiz [UNESP]2014-05-27T11:29:53Z2014-05-27T11:29:53Z2013-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article496-501application/pdfhttp://dx.doi.org/10.1590/S0102-86502013000700004Acta Cirurgica Brasileira, v. 28, n. 7, p. 496-501, 2013.0102-86501678-2674http://hdl.handle.net/11449/7584210.1590/S0102-86502013000700004S0102-86502013000700004WOS:0003224070000042-s2.0-848803934312-s2.0-84880393431.pdf2443296326760741872789708052228999898578543556920000-0001-8411-5822Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengActa Cirúrgica Brasileira0.9330,395info:eu-repo/semantics/openAccess2024-09-03T14:30:00Zoai:repositorio.unesp.br:11449/75842Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-03T14:30Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Nephrocalcinosis induced by hyperoxaluria in rats |
title |
Nephrocalcinosis induced by hyperoxaluria in rats |
spellingShingle |
Nephrocalcinosis induced by hyperoxaluria in rats Cunha, Natália Baraldi [UNESP] Hyperoxaluria Lithiasis Nephrocalcinosis Rats calcium calcium oxalate citric acid creatinine oxalic acid uric acid animal experiment animal model animal tissue controlled study histology hyperoxaluria kidney calcification kidney parenchyma male morphometrics nonhuman rat urine pH urine volume |
title_short |
Nephrocalcinosis induced by hyperoxaluria in rats |
title_full |
Nephrocalcinosis induced by hyperoxaluria in rats |
title_fullStr |
Nephrocalcinosis induced by hyperoxaluria in rats |
title_full_unstemmed |
Nephrocalcinosis induced by hyperoxaluria in rats |
title_sort |
Nephrocalcinosis induced by hyperoxaluria in rats |
author |
Cunha, Natália Baraldi [UNESP] |
author_facet |
Cunha, Natália Baraldi [UNESP] Kawano, Paulo Roberto [UNESP] Padovani, Carlos Roberto [UNESP] Lima, Flávio de Oliveira [UNESP] Bernardes, Suene Magalhães, Eloá Siqueira [UNESP] Amaro, Carmen Regina Petean [UNESP] Amaro, João Luiz [UNESP] |
author_role |
author |
author2 |
Kawano, Paulo Roberto [UNESP] Padovani, Carlos Roberto [UNESP] Lima, Flávio de Oliveira [UNESP] Bernardes, Suene Magalhães, Eloá Siqueira [UNESP] Amaro, Carmen Regina Petean [UNESP] Amaro, João Luiz [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Cunha, Natália Baraldi [UNESP] Kawano, Paulo Roberto [UNESP] Padovani, Carlos Roberto [UNESP] Lima, Flávio de Oliveira [UNESP] Bernardes, Suene Magalhães, Eloá Siqueira [UNESP] Amaro, Carmen Regina Petean [UNESP] Amaro, João Luiz [UNESP] |
dc.subject.por.fl_str_mv |
Hyperoxaluria Lithiasis Nephrocalcinosis Rats calcium calcium oxalate citric acid creatinine oxalic acid uric acid animal experiment animal model animal tissue controlled study histology hyperoxaluria kidney calcification kidney parenchyma male morphometrics nonhuman rat urine pH urine volume |
topic |
Hyperoxaluria Lithiasis Nephrocalcinosis Rats calcium calcium oxalate citric acid creatinine oxalic acid uric acid animal experiment animal model animal tissue controlled study histology hyperoxaluria kidney calcification kidney parenchyma male morphometrics nonhuman rat urine pH urine volume |
description |
Purpose: To investigate nephrocalcinosis due to hyperoxaluria induced by two different inducing agents in rats. Methods: Forty Sprague-Dawley male rats were randomly distributed into four groups: Group1 (Clinical control, n = 10); Group 2 (0.5% Ethylene Glycol + Vitamin D3, n = 10); Group 3 (1.25% Ethylene Glycol, n = 10); and Group 4 (5%Hydroxy L-proline, n = 10). Five animals from each group were euthanized after one week of follow-up (M1 Moment) and the remaining, after four weeks (M2 Moment). All animals underwent 24h urine dosages of calcium, oxalate, uric acid, citrate and serum creatinine. Histology and histomorphometric analyses were performed using Image J program in the hematoxylin-eosin stains. Calcium deposits in the renal parenchyma were quantified by PIXE technique (Proton Induced X-Ray Emission). Results: 24h urinary parameters did not show any significant variations after 28 days of experiment except by hyperoxaluria that was significantly higher in Group 3. Histomorphometric analyses showed a significantly higher nephrocalcinosis in Group 2 (p<0.01). The calcium deposits in the renal parenchyma were 10 and 100 times higher in Group 2 in comparison to other groups in the M1 and M2 moments, respectively. Conclusion: The Group 2 (vitamin D3+Ethylene Glycol 0.5%) was the best model to induce nephrocalcinosis in rats after 28 days. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-07-01 2014-05-27T11:29:53Z 2014-05-27T11:29:53Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S0102-86502013000700004 Acta Cirurgica Brasileira, v. 28, n. 7, p. 496-501, 2013. 0102-8650 1678-2674 http://hdl.handle.net/11449/75842 10.1590/S0102-86502013000700004 S0102-86502013000700004 WOS:000322407000004 2-s2.0-84880393431 2-s2.0-84880393431.pdf 2443296326760741 8727897080522289 9989857854355692 0000-0001-8411-5822 |
url |
http://dx.doi.org/10.1590/S0102-86502013000700004 http://hdl.handle.net/11449/75842 |
identifier_str_mv |
Acta Cirurgica Brasileira, v. 28, n. 7, p. 496-501, 2013. 0102-8650 1678-2674 10.1590/S0102-86502013000700004 S0102-86502013000700004 WOS:000322407000004 2-s2.0-84880393431 2-s2.0-84880393431.pdf 2443296326760741 8727897080522289 9989857854355692 0000-0001-8411-5822 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Acta Cirúrgica Brasileira 0.933 0,395 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
496-501 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1810021383532445696 |