Enhancement of salt tolerance in corn using Azospirillum brasilense: an approach on antioxidant systems
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s10265-021-01332-1 http://hdl.handle.net/11449/229210 |
Resumo: | Salinity has become one of the major factors limiting agricultural production. In this regard, different cost-effective management strategies such as the use of plant growth-promoting bacteria (PGPB) as inoculants to alleviate salt-stress conditions and minimize plant productivity losses have been used in agricultural systems. The aim of this study was to characterize induced antioxidant responses in corn through inoculation with Azospirillum brasilense and examine the relationship between these responses and the acquired salt-stress tolerance. Treatments were performed by combining sodium chloride (0 and 100 mM NaCl) through irrigation water with absence and presence of A. brasilense inoculation. The experiment was performed in a completely randomized design with four replications. Lipid peroxidation (malondialdehyde [MDA]), and nitrogen (N), sodium (Na+) and potassium (K+) contents, as well as dry biomass, glycine betaine, and antioxidant enzymes activities such as of superoxide dismutase (SOD, EC 1. 15. 1. 1), glutathione reductase (GR, EC 1. 6. 4. 2), guaiacol peroxidase (GPOX, EC 1. 11. 1. 7), and glutathione peroxidase (GSH-PX, EC 1. 11. 1. 9) were determined. Overall results indicated that plants treated with 100 mM NaCl showed the most pronounced salt-stress damages with consequent increase in MDA content. However, inoculated plants showed an enhanced capacity to withstand or avoid salt-stress damages. These results could be attributed, at least in part, to the increased activity of antioxidant enzymes. Our results suggest that A. brasilense may confer tolerance to salt stress in corn plants enhancing antioxidant responses, primarily by the enzymes GSH-PX and GPOX, and the osmolyte glycine betaine. |
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Enhancement of salt tolerance in corn using Azospirillum brasilense: an approach on antioxidant systemsAlleviateAntioxidant enzymesGrowth-promoting bacteriaOxidative stressSalinitySalinity has become one of the major factors limiting agricultural production. In this regard, different cost-effective management strategies such as the use of plant growth-promoting bacteria (PGPB) as inoculants to alleviate salt-stress conditions and minimize plant productivity losses have been used in agricultural systems. The aim of this study was to characterize induced antioxidant responses in corn through inoculation with Azospirillum brasilense and examine the relationship between these responses and the acquired salt-stress tolerance. Treatments were performed by combining sodium chloride (0 and 100 mM NaCl) through irrigation water with absence and presence of A. brasilense inoculation. The experiment was performed in a completely randomized design with four replications. Lipid peroxidation (malondialdehyde [MDA]), and nitrogen (N), sodium (Na+) and potassium (K+) contents, as well as dry biomass, glycine betaine, and antioxidant enzymes activities such as of superoxide dismutase (SOD, EC 1. 15. 1. 1), glutathione reductase (GR, EC 1. 6. 4. 2), guaiacol peroxidase (GPOX, EC 1. 11. 1. 7), and glutathione peroxidase (GSH-PX, EC 1. 11. 1. 9) were determined. Overall results indicated that plants treated with 100 mM NaCl showed the most pronounced salt-stress damages with consequent increase in MDA content. However, inoculated plants showed an enhanced capacity to withstand or avoid salt-stress damages. These results could be attributed, at least in part, to the increased activity of antioxidant enzymes. Our results suggest that A. brasilense may confer tolerance to salt stress in corn plants enhancing antioxidant responses, primarily by the enzymes GSH-PX and GPOX, and the osmolyte glycine betaine.Universidade Estadual Paulista (UNESP) Faculdade de Ciências Agrárias e Veterinárias Depto. de Biologia Aplicada à AgropecuáriaNúcleo de Produção Vegetal Instituto Nacional do Semiárido (INSA)Universidade Estadual Paulista (UNESP) Faculdade de Ciências Agrárias e Veterinárias Depto. de Produção VegetalUniversidade Estadual Paulista (UNESP) Faculdade de Ciências Agrárias e Veterinárias Depto. de Biologia Aplicada à AgropecuáriaUniversidade Estadual Paulista (UNESP) Faculdade de Ciências Agrárias e Veterinárias Depto. de Produção VegetalUniversidade Estadual Paulista (UNESP)Instituto Nacional do Semiárido (INSA)Checchio, Mirela Vantini [UNESP]de Cássia Alves, Ritade Oliveira, Kevein Ruas [UNESP]Moro, Gustavo Vitti [UNESP]Santos, Durvalina Maria Mathias dos [UNESP]Gratão, Priscila Lupino [UNESP]2022-04-29T08:31:15Z2022-04-29T08:31:15Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1007/s10265-021-01332-1Journal of Plant Research.1618-08600918-9440http://hdl.handle.net/11449/22921010.1007/s10265-021-01332-12-s2.0-85111166291Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Plant Researchinfo:eu-repo/semantics/openAccess2024-06-07T13:56:41Zoai:repositorio.unesp.br:11449/229210Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:26:21.019668Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Enhancement of salt tolerance in corn using Azospirillum brasilense: an approach on antioxidant systems |
title |
Enhancement of salt tolerance in corn using Azospirillum brasilense: an approach on antioxidant systems |
spellingShingle |
Enhancement of salt tolerance in corn using Azospirillum brasilense: an approach on antioxidant systems Checchio, Mirela Vantini [UNESP] Alleviate Antioxidant enzymes Growth-promoting bacteria Oxidative stress Salinity |
title_short |
Enhancement of salt tolerance in corn using Azospirillum brasilense: an approach on antioxidant systems |
title_full |
Enhancement of salt tolerance in corn using Azospirillum brasilense: an approach on antioxidant systems |
title_fullStr |
Enhancement of salt tolerance in corn using Azospirillum brasilense: an approach on antioxidant systems |
title_full_unstemmed |
Enhancement of salt tolerance in corn using Azospirillum brasilense: an approach on antioxidant systems |
title_sort |
Enhancement of salt tolerance in corn using Azospirillum brasilense: an approach on antioxidant systems |
author |
Checchio, Mirela Vantini [UNESP] |
author_facet |
Checchio, Mirela Vantini [UNESP] de Cássia Alves, Rita de Oliveira, Kevein Ruas [UNESP] Moro, Gustavo Vitti [UNESP] Santos, Durvalina Maria Mathias dos [UNESP] Gratão, Priscila Lupino [UNESP] |
author_role |
author |
author2 |
de Cássia Alves, Rita de Oliveira, Kevein Ruas [UNESP] Moro, Gustavo Vitti [UNESP] Santos, Durvalina Maria Mathias dos [UNESP] Gratão, Priscila Lupino [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Instituto Nacional do Semiárido (INSA) |
dc.contributor.author.fl_str_mv |
Checchio, Mirela Vantini [UNESP] de Cássia Alves, Rita de Oliveira, Kevein Ruas [UNESP] Moro, Gustavo Vitti [UNESP] Santos, Durvalina Maria Mathias dos [UNESP] Gratão, Priscila Lupino [UNESP] |
dc.subject.por.fl_str_mv |
Alleviate Antioxidant enzymes Growth-promoting bacteria Oxidative stress Salinity |
topic |
Alleviate Antioxidant enzymes Growth-promoting bacteria Oxidative stress Salinity |
description |
Salinity has become one of the major factors limiting agricultural production. In this regard, different cost-effective management strategies such as the use of plant growth-promoting bacteria (PGPB) as inoculants to alleviate salt-stress conditions and minimize plant productivity losses have been used in agricultural systems. The aim of this study was to characterize induced antioxidant responses in corn through inoculation with Azospirillum brasilense and examine the relationship between these responses and the acquired salt-stress tolerance. Treatments were performed by combining sodium chloride (0 and 100 mM NaCl) through irrigation water with absence and presence of A. brasilense inoculation. The experiment was performed in a completely randomized design with four replications. Lipid peroxidation (malondialdehyde [MDA]), and nitrogen (N), sodium (Na+) and potassium (K+) contents, as well as dry biomass, glycine betaine, and antioxidant enzymes activities such as of superoxide dismutase (SOD, EC 1. 15. 1. 1), glutathione reductase (GR, EC 1. 6. 4. 2), guaiacol peroxidase (GPOX, EC 1. 11. 1. 7), and glutathione peroxidase (GSH-PX, EC 1. 11. 1. 9) were determined. Overall results indicated that plants treated with 100 mM NaCl showed the most pronounced salt-stress damages with consequent increase in MDA content. However, inoculated plants showed an enhanced capacity to withstand or avoid salt-stress damages. These results could be attributed, at least in part, to the increased activity of antioxidant enzymes. Our results suggest that A. brasilense may confer tolerance to salt stress in corn plants enhancing antioxidant responses, primarily by the enzymes GSH-PX and GPOX, and the osmolyte glycine betaine. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-01-01 2022-04-29T08:31:15Z 2022-04-29T08:31:15Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s10265-021-01332-1 Journal of Plant Research. 1618-0860 0918-9440 http://hdl.handle.net/11449/229210 10.1007/s10265-021-01332-1 2-s2.0-85111166291 |
url |
http://dx.doi.org/10.1007/s10265-021-01332-1 http://hdl.handle.net/11449/229210 |
identifier_str_mv |
Journal of Plant Research. 1618-0860 0918-9440 10.1007/s10265-021-01332-1 2-s2.0-85111166291 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Plant Research |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129202250055680 |