An integrated approach to obtain xylo-oligosaccharides from sugarcane straw: From lab to pilot scale
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.biortech.2020.123637 http://hdl.handle.net/11449/199018 |
Resumo: | Sugarcane straw (SS) is a widely available agricultural processing feedstock with the potential to produce 2nd generation bioethanol and bioproducts, in addition to the more conventional use for heat and/or electrical power generation. In this study, we investigated the operational parameters to maximize the production of xylo-oligosaccharides (XOS) using mild deacetylation, followed by hydrothermal pretreatment. From the laboratory to the pilot-scale, the optimized two-stage pretreatment promoted 81.5% and 70.5% hemicellulose solubilization and led to XOS yields up to 9.8% and 9.1% (w/w of initial straw), respectively. Moreover, different fungal xylanases were also tested to hydrolyze XOS into xylobiose (X2) and xylotriose (X3). GH10 from Aspergillus nidulans performed better than GH11 xylanases and the ratio of the desired products (X2 + X3) increased to 72% due to minimal monomeric sugar formation. Furthermore, a cellulose-rich fraction was obtained, which can be used in other high value-added applications, such as for the production of cello-oligomers. |
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An integrated approach to obtain xylo-oligosaccharides from sugarcane straw: From lab to pilot scaleDeacetylationHydrothermal pretreatmentSugarcane strawXylanasesXylo-oligosaccharidesSugarcane straw (SS) is a widely available agricultural processing feedstock with the potential to produce 2nd generation bioethanol and bioproducts, in addition to the more conventional use for heat and/or electrical power generation. In this study, we investigated the operational parameters to maximize the production of xylo-oligosaccharides (XOS) using mild deacetylation, followed by hydrothermal pretreatment. From the laboratory to the pilot-scale, the optimized two-stage pretreatment promoted 81.5% and 70.5% hemicellulose solubilization and led to XOS yields up to 9.8% and 9.1% (w/w of initial straw), respectively. Moreover, different fungal xylanases were also tested to hydrolyze XOS into xylobiose (X2) and xylotriose (X3). GH10 from Aspergillus nidulans performed better than GH11 xylanases and the ratio of the desired products (X2 + X3) increased to 72% due to minimal monomeric sugar formation. Furthermore, a cellulose-rich fraction was obtained, which can be used in other high value-added applications, such as for the production of cello-oligomers.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Biotechnology and Biological Sciences Research CouncilConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Brazilian Biorenewables National Laboratory (LNBR) Brazilian Center for Research in Energy and Materials (CNPEM), Giuseppe Máximo Scolfaro, 10.000Interdisciplinary Center of Energy Planning University of Campinas, Cora Coralina, 330Department of Biochemistry and Tissue Biology Institute of Biology University of Campinas (UNICAMP)Department of Bioprocess and Biotechnology College of Agricultural Sciences São Paulo State University (UNESP), Avenida UniversitáriaDepartment of Bioprocess and Biotechnology College of Agricultural Sciences São Paulo State University (UNESP), Avenida UniversitáriaFAPESP: 2015/50612-8Biotechnology and Biological Sciences Research Council: 2017/15477-8Biotechnology and Biological Sciences Research Council: 2017/22669-0CNPq: 304816/2017-5CNPq: 304944/2018-1CNPq: 404654/2018-5Brazilian Center for Research in Energy and Materials (CNPEM)Universidade Estadual de Campinas (UNICAMP)Universidade Estadual Paulista (Unesp)Brenelli, Lívia B.Figueiredo, Fernanda L.Damasio, AndréFranco, Telma T.Rabelo, Sarita C. [UNESP]2020-12-12T01:28:25Z2020-12-12T01:28:25Z2020-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.biortech.2020.123637Bioresource Technology, v. 313.1873-29760960-8524http://hdl.handle.net/11449/19901810.1016/j.biortech.2020.1236372-s2.0-85086761985Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBioresource Technologyinfo:eu-repo/semantics/openAccess2021-10-22T22:23:55Zoai:repositorio.unesp.br:11449/199018Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:11:14.770916Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
An integrated approach to obtain xylo-oligosaccharides from sugarcane straw: From lab to pilot scale |
title |
An integrated approach to obtain xylo-oligosaccharides from sugarcane straw: From lab to pilot scale |
spellingShingle |
An integrated approach to obtain xylo-oligosaccharides from sugarcane straw: From lab to pilot scale Brenelli, Lívia B. Deacetylation Hydrothermal pretreatment Sugarcane straw Xylanases Xylo-oligosaccharides |
title_short |
An integrated approach to obtain xylo-oligosaccharides from sugarcane straw: From lab to pilot scale |
title_full |
An integrated approach to obtain xylo-oligosaccharides from sugarcane straw: From lab to pilot scale |
title_fullStr |
An integrated approach to obtain xylo-oligosaccharides from sugarcane straw: From lab to pilot scale |
title_full_unstemmed |
An integrated approach to obtain xylo-oligosaccharides from sugarcane straw: From lab to pilot scale |
title_sort |
An integrated approach to obtain xylo-oligosaccharides from sugarcane straw: From lab to pilot scale |
author |
Brenelli, Lívia B. |
author_facet |
Brenelli, Lívia B. Figueiredo, Fernanda L. Damasio, André Franco, Telma T. Rabelo, Sarita C. [UNESP] |
author_role |
author |
author2 |
Figueiredo, Fernanda L. Damasio, André Franco, Telma T. Rabelo, Sarita C. [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Brazilian Center for Research in Energy and Materials (CNPEM) Universidade Estadual de Campinas (UNICAMP) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Brenelli, Lívia B. Figueiredo, Fernanda L. Damasio, André Franco, Telma T. Rabelo, Sarita C. [UNESP] |
dc.subject.por.fl_str_mv |
Deacetylation Hydrothermal pretreatment Sugarcane straw Xylanases Xylo-oligosaccharides |
topic |
Deacetylation Hydrothermal pretreatment Sugarcane straw Xylanases Xylo-oligosaccharides |
description |
Sugarcane straw (SS) is a widely available agricultural processing feedstock with the potential to produce 2nd generation bioethanol and bioproducts, in addition to the more conventional use for heat and/or electrical power generation. In this study, we investigated the operational parameters to maximize the production of xylo-oligosaccharides (XOS) using mild deacetylation, followed by hydrothermal pretreatment. From the laboratory to the pilot-scale, the optimized two-stage pretreatment promoted 81.5% and 70.5% hemicellulose solubilization and led to XOS yields up to 9.8% and 9.1% (w/w of initial straw), respectively. Moreover, different fungal xylanases were also tested to hydrolyze XOS into xylobiose (X2) and xylotriose (X3). GH10 from Aspergillus nidulans performed better than GH11 xylanases and the ratio of the desired products (X2 + X3) increased to 72% due to minimal monomeric sugar formation. Furthermore, a cellulose-rich fraction was obtained, which can be used in other high value-added applications, such as for the production of cello-oligomers. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T01:28:25Z 2020-12-12T01:28:25Z 2020-10-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.biortech.2020.123637 Bioresource Technology, v. 313. 1873-2976 0960-8524 http://hdl.handle.net/11449/199018 10.1016/j.biortech.2020.123637 2-s2.0-85086761985 |
url |
http://dx.doi.org/10.1016/j.biortech.2020.123637 http://hdl.handle.net/11449/199018 |
identifier_str_mv |
Bioresource Technology, v. 313. 1873-2976 0960-8524 10.1016/j.biortech.2020.123637 2-s2.0-85086761985 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Bioresource Technology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128615999602688 |