An integrated approach to obtain xylo-oligosaccharides from sugarcane straw: From lab to pilot scale

Detalhes bibliográficos
Autor(a) principal: Brenelli, Lívia B.
Data de Publicação: 2020
Outros Autores: Figueiredo, Fernanda L., Damasio, André, Franco, Telma T., Rabelo, Sarita C. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.biortech.2020.123637
http://hdl.handle.net/11449/199018
Resumo: Sugarcane straw (SS) is a widely available agricultural processing feedstock with the potential to produce 2nd generation bioethanol and bioproducts, in addition to the more conventional use for heat and/or electrical power generation. In this study, we investigated the operational parameters to maximize the production of xylo-oligosaccharides (XOS) using mild deacetylation, followed by hydrothermal pretreatment. From the laboratory to the pilot-scale, the optimized two-stage pretreatment promoted 81.5% and 70.5% hemicellulose solubilization and led to XOS yields up to 9.8% and 9.1% (w/w of initial straw), respectively. Moreover, different fungal xylanases were also tested to hydrolyze XOS into xylobiose (X2) and xylotriose (X3). GH10 from Aspergillus nidulans performed better than GH11 xylanases and the ratio of the desired products (X2 + X3) increased to 72% due to minimal monomeric sugar formation. Furthermore, a cellulose-rich fraction was obtained, which can be used in other high value-added applications, such as for the production of cello-oligomers.
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spelling An integrated approach to obtain xylo-oligosaccharides from sugarcane straw: From lab to pilot scaleDeacetylationHydrothermal pretreatmentSugarcane strawXylanasesXylo-oligosaccharidesSugarcane straw (SS) is a widely available agricultural processing feedstock with the potential to produce 2nd generation bioethanol and bioproducts, in addition to the more conventional use for heat and/or electrical power generation. In this study, we investigated the operational parameters to maximize the production of xylo-oligosaccharides (XOS) using mild deacetylation, followed by hydrothermal pretreatment. From the laboratory to the pilot-scale, the optimized two-stage pretreatment promoted 81.5% and 70.5% hemicellulose solubilization and led to XOS yields up to 9.8% and 9.1% (w/w of initial straw), respectively. Moreover, different fungal xylanases were also tested to hydrolyze XOS into xylobiose (X2) and xylotriose (X3). GH10 from Aspergillus nidulans performed better than GH11 xylanases and the ratio of the desired products (X2 + X3) increased to 72% due to minimal monomeric sugar formation. Furthermore, a cellulose-rich fraction was obtained, which can be used in other high value-added applications, such as for the production of cello-oligomers.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Biotechnology and Biological Sciences Research CouncilConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Brazilian Biorenewables National Laboratory (LNBR) Brazilian Center for Research in Energy and Materials (CNPEM), Giuseppe Máximo Scolfaro, 10.000Interdisciplinary Center of Energy Planning University of Campinas, Cora Coralina, 330Department of Biochemistry and Tissue Biology Institute of Biology University of Campinas (UNICAMP)Department of Bioprocess and Biotechnology College of Agricultural Sciences São Paulo State University (UNESP), Avenida UniversitáriaDepartment of Bioprocess and Biotechnology College of Agricultural Sciences São Paulo State University (UNESP), Avenida UniversitáriaFAPESP: 2015/50612-8Biotechnology and Biological Sciences Research Council: 2017/15477-8Biotechnology and Biological Sciences Research Council: 2017/22669-0CNPq: 304816/2017-5CNPq: 304944/2018-1CNPq: 404654/2018-5Brazilian Center for Research in Energy and Materials (CNPEM)Universidade Estadual de Campinas (UNICAMP)Universidade Estadual Paulista (Unesp)Brenelli, Lívia B.Figueiredo, Fernanda L.Damasio, AndréFranco, Telma T.Rabelo, Sarita C. [UNESP]2020-12-12T01:28:25Z2020-12-12T01:28:25Z2020-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.biortech.2020.123637Bioresource Technology, v. 313.1873-29760960-8524http://hdl.handle.net/11449/19901810.1016/j.biortech.2020.1236372-s2.0-85086761985Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBioresource Technologyinfo:eu-repo/semantics/openAccess2021-10-22T22:23:55Zoai:repositorio.unesp.br:11449/199018Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:11:14.770916Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv An integrated approach to obtain xylo-oligosaccharides from sugarcane straw: From lab to pilot scale
title An integrated approach to obtain xylo-oligosaccharides from sugarcane straw: From lab to pilot scale
spellingShingle An integrated approach to obtain xylo-oligosaccharides from sugarcane straw: From lab to pilot scale
Brenelli, Lívia B.
Deacetylation
Hydrothermal pretreatment
Sugarcane straw
Xylanases
Xylo-oligosaccharides
title_short An integrated approach to obtain xylo-oligosaccharides from sugarcane straw: From lab to pilot scale
title_full An integrated approach to obtain xylo-oligosaccharides from sugarcane straw: From lab to pilot scale
title_fullStr An integrated approach to obtain xylo-oligosaccharides from sugarcane straw: From lab to pilot scale
title_full_unstemmed An integrated approach to obtain xylo-oligosaccharides from sugarcane straw: From lab to pilot scale
title_sort An integrated approach to obtain xylo-oligosaccharides from sugarcane straw: From lab to pilot scale
author Brenelli, Lívia B.
author_facet Brenelli, Lívia B.
Figueiredo, Fernanda L.
Damasio, André
Franco, Telma T.
Rabelo, Sarita C. [UNESP]
author_role author
author2 Figueiredo, Fernanda L.
Damasio, André
Franco, Telma T.
Rabelo, Sarita C. [UNESP]
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Brazilian Center for Research in Energy and Materials (CNPEM)
Universidade Estadual de Campinas (UNICAMP)
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Brenelli, Lívia B.
Figueiredo, Fernanda L.
Damasio, André
Franco, Telma T.
Rabelo, Sarita C. [UNESP]
dc.subject.por.fl_str_mv Deacetylation
Hydrothermal pretreatment
Sugarcane straw
Xylanases
Xylo-oligosaccharides
topic Deacetylation
Hydrothermal pretreatment
Sugarcane straw
Xylanases
Xylo-oligosaccharides
description Sugarcane straw (SS) is a widely available agricultural processing feedstock with the potential to produce 2nd generation bioethanol and bioproducts, in addition to the more conventional use for heat and/or electrical power generation. In this study, we investigated the operational parameters to maximize the production of xylo-oligosaccharides (XOS) using mild deacetylation, followed by hydrothermal pretreatment. From the laboratory to the pilot-scale, the optimized two-stage pretreatment promoted 81.5% and 70.5% hemicellulose solubilization and led to XOS yields up to 9.8% and 9.1% (w/w of initial straw), respectively. Moreover, different fungal xylanases were also tested to hydrolyze XOS into xylobiose (X2) and xylotriose (X3). GH10 from Aspergillus nidulans performed better than GH11 xylanases and the ratio of the desired products (X2 + X3) increased to 72% due to minimal monomeric sugar formation. Furthermore, a cellulose-rich fraction was obtained, which can be used in other high value-added applications, such as for the production of cello-oligomers.
publishDate 2020
dc.date.none.fl_str_mv 2020-12-12T01:28:25Z
2020-12-12T01:28:25Z
2020-10-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.biortech.2020.123637
Bioresource Technology, v. 313.
1873-2976
0960-8524
http://hdl.handle.net/11449/199018
10.1016/j.biortech.2020.123637
2-s2.0-85086761985
url http://dx.doi.org/10.1016/j.biortech.2020.123637
http://hdl.handle.net/11449/199018
identifier_str_mv Bioresource Technology, v. 313.
1873-2976
0960-8524
10.1016/j.biortech.2020.123637
2-s2.0-85086761985
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Bioresource Technology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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