Evaluation of the effects of photodynamic therapy with hypericin-glucamine in the treatment of periodontal disease induced in rats
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s41547-018-0045-0 http://hdl.handle.net/11449/230591 |
Resumo: | Purpose: The purpose of this study was to evaluate the effectiveness of a photosensitizer hypericin-glucamine, activated by LED amber (34.10 J/cm2) as an adjuvant to scaling and root planing (SRP), on the treatment of experimental periodontal disease (PD) in rats. Methods: In a 15-day period, PD was induced in the mandibular molars through ligature placement. The animals (n = 60) were randomly divided into four groups: control (with induction of periodontal disease), scaling and root planing (SRP), antimicrobial photodynamic therapy (aPDT), and SRP + aPDT. At 7, 15, and 30 days after treatments, animals were euthanized. Digital microtomography, histometric, and stereometric analyses were performed to calculate the bone loss of mandibular second molars, and the tissue repair was analyzed histologically. The data were subjected to statistical analysis (α = 5%). Results: Histologically, the control group periodontium showed several morphological changes, but an evident gradual reduction in the inflammatory process was observed in the subsequent periods. The SRP, aPDT, and SRP + aPDT groups presented the same, but in less intensity. The stereometric analysis showed a significantly higher proportion of fibroblasts in SRP group (p < 0,001) and SRP + aPDT group (p < 0.0001) compared to that in the control group at 7 days post treatment. Conclusions: We conclude that scaling and root planing with or without photodynamic therapy with hypericin-glucamine increased density of the fibroblast and cell density. However, there is no statistically significant difference between SRP and SRP + aPDT. |
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Evaluation of the effects of photodynamic therapy with hypericin-glucamine in the treatment of periodontal disease induced in ratsHypericin-glucaminePeriodontal diseaseScaling and root planingPurpose: The purpose of this study was to evaluate the effectiveness of a photosensitizer hypericin-glucamine, activated by LED amber (34.10 J/cm2) as an adjuvant to scaling and root planing (SRP), on the treatment of experimental periodontal disease (PD) in rats. Methods: In a 15-day period, PD was induced in the mandibular molars through ligature placement. The animals (n = 60) were randomly divided into four groups: control (with induction of periodontal disease), scaling and root planing (SRP), antimicrobial photodynamic therapy (aPDT), and SRP + aPDT. At 7, 15, and 30 days after treatments, animals were euthanized. Digital microtomography, histometric, and stereometric analyses were performed to calculate the bone loss of mandibular second molars, and the tissue repair was analyzed histologically. The data were subjected to statistical analysis (α = 5%). Results: Histologically, the control group periodontium showed several morphological changes, but an evident gradual reduction in the inflammatory process was observed in the subsequent periods. The SRP, aPDT, and SRP + aPDT groups presented the same, but in less intensity. The stereometric analysis showed a significantly higher proportion of fibroblasts in SRP group (p < 0,001) and SRP + aPDT group (p < 0.0001) compared to that in the control group at 7 days post treatment. Conclusions: We conclude that scaling and root planing with or without photodynamic therapy with hypericin-glucamine increased density of the fibroblast and cell density. However, there is no statistically significant difference between SRP and SRP + aPDT.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)School of Dentistry Araraquara Department of Diagnosis and Surgery São Paulo State University (Unesp), Rua Humaitá, 1680, SPChemistry and Molecular Physics Department USP São Paulo University, SPCentre for Natural Sciences and Humanities UFABC Federal University of ABC, SPSchool of Dentistry Araraquara Department of Diagnosis and Surgery São Paulo State University (Unesp), Rua Humaitá, 1680, SPCNPq: 133436/2012-8CAPES: AUXPG 655/2014Universidade Estadual Paulista (UNESP)Universidade de São Paulo (USP)Universidade Federal do ABC (UFABC)Macedo, Paula Delello [UNESP]Corbi, Sâmara Tfaile [UNESP]Kitagawa, Fernanda Ali [UNESP]Perussi, Janice RodriguesRibeiro, Anderson OrzariMarcantonio, Rosemary Adriana Chierici [UNESP]2022-04-29T08:40:53Z2022-04-29T08:40:53Z2018-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article255-263http://dx.doi.org/10.1007/s41547-018-0045-0Lasers in Dental Science, v. 2, n. 4, p. 255-263, 2018.2367-2587http://hdl.handle.net/11449/23059110.1007/s41547-018-0045-02-s2.0-85126542782Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengLasers in Dental Scienceinfo:eu-repo/semantics/openAccess2022-04-29T08:40:53Zoai:repositorio.unesp.br:11449/230591Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:33:56.166419Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Evaluation of the effects of photodynamic therapy with hypericin-glucamine in the treatment of periodontal disease induced in rats |
title |
Evaluation of the effects of photodynamic therapy with hypericin-glucamine in the treatment of periodontal disease induced in rats |
spellingShingle |
Evaluation of the effects of photodynamic therapy with hypericin-glucamine in the treatment of periodontal disease induced in rats Macedo, Paula Delello [UNESP] Hypericin-glucamine Periodontal disease Scaling and root planing |
title_short |
Evaluation of the effects of photodynamic therapy with hypericin-glucamine in the treatment of periodontal disease induced in rats |
title_full |
Evaluation of the effects of photodynamic therapy with hypericin-glucamine in the treatment of periodontal disease induced in rats |
title_fullStr |
Evaluation of the effects of photodynamic therapy with hypericin-glucamine in the treatment of periodontal disease induced in rats |
title_full_unstemmed |
Evaluation of the effects of photodynamic therapy with hypericin-glucamine in the treatment of periodontal disease induced in rats |
title_sort |
Evaluation of the effects of photodynamic therapy with hypericin-glucamine in the treatment of periodontal disease induced in rats |
author |
Macedo, Paula Delello [UNESP] |
author_facet |
Macedo, Paula Delello [UNESP] Corbi, Sâmara Tfaile [UNESP] Kitagawa, Fernanda Ali [UNESP] Perussi, Janice Rodrigues Ribeiro, Anderson Orzari Marcantonio, Rosemary Adriana Chierici [UNESP] |
author_role |
author |
author2 |
Corbi, Sâmara Tfaile [UNESP] Kitagawa, Fernanda Ali [UNESP] Perussi, Janice Rodrigues Ribeiro, Anderson Orzari Marcantonio, Rosemary Adriana Chierici [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Universidade de São Paulo (USP) Universidade Federal do ABC (UFABC) |
dc.contributor.author.fl_str_mv |
Macedo, Paula Delello [UNESP] Corbi, Sâmara Tfaile [UNESP] Kitagawa, Fernanda Ali [UNESP] Perussi, Janice Rodrigues Ribeiro, Anderson Orzari Marcantonio, Rosemary Adriana Chierici [UNESP] |
dc.subject.por.fl_str_mv |
Hypericin-glucamine Periodontal disease Scaling and root planing |
topic |
Hypericin-glucamine Periodontal disease Scaling and root planing |
description |
Purpose: The purpose of this study was to evaluate the effectiveness of a photosensitizer hypericin-glucamine, activated by LED amber (34.10 J/cm2) as an adjuvant to scaling and root planing (SRP), on the treatment of experimental periodontal disease (PD) in rats. Methods: In a 15-day period, PD was induced in the mandibular molars through ligature placement. The animals (n = 60) were randomly divided into four groups: control (with induction of periodontal disease), scaling and root planing (SRP), antimicrobial photodynamic therapy (aPDT), and SRP + aPDT. At 7, 15, and 30 days after treatments, animals were euthanized. Digital microtomography, histometric, and stereometric analyses were performed to calculate the bone loss of mandibular second molars, and the tissue repair was analyzed histologically. The data were subjected to statistical analysis (α = 5%). Results: Histologically, the control group periodontium showed several morphological changes, but an evident gradual reduction in the inflammatory process was observed in the subsequent periods. The SRP, aPDT, and SRP + aPDT groups presented the same, but in less intensity. The stereometric analysis showed a significantly higher proportion of fibroblasts in SRP group (p < 0,001) and SRP + aPDT group (p < 0.0001) compared to that in the control group at 7 days post treatment. Conclusions: We conclude that scaling and root planing with or without photodynamic therapy with hypericin-glucamine increased density of the fibroblast and cell density. However, there is no statistically significant difference between SRP and SRP + aPDT. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-01 2022-04-29T08:40:53Z 2022-04-29T08:40:53Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s41547-018-0045-0 Lasers in Dental Science, v. 2, n. 4, p. 255-263, 2018. 2367-2587 http://hdl.handle.net/11449/230591 10.1007/s41547-018-0045-0 2-s2.0-85126542782 |
url |
http://dx.doi.org/10.1007/s41547-018-0045-0 http://hdl.handle.net/11449/230591 |
identifier_str_mv |
Lasers in Dental Science, v. 2, n. 4, p. 255-263, 2018. 2367-2587 10.1007/s41547-018-0045-0 2-s2.0-85126542782 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Lasers in Dental Science |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
255-263 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128533975793664 |