Rheological and structural characterization of gels from albumin and low methoxyl amidated pectin mixtures

Detalhes bibliográficos
Autor(a) principal: Chaux-Gutiérrez, Ana María [UNESP]
Data de Publicação: 2019
Outros Autores: Pérez-Monterroza, Ezequiel José [UNESP], Mauro, Maria Aparecida [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.foodhyd.2019.01.025
http://hdl.handle.net/11449/188688
Resumo: The rheological behavior of gels prepared from albumin (ALB) and a mixture of ALB and low methoxyl amidated pectin (PEC) with different proportions were studied using oscillatory rheological tests, Fourier transform infrared spectroscopy (FT-IR spectroscopy), zeta potential measurements and confocal microscopy. The mixture was always 5% of the total solute. Assays were performed in two series, one in which all mixtures were adjusted to pH = 8 and another one without pH adjustment. The zeta potential values indicated the presence of a negative net charge on ALB-PEC gels. The rheological tests indicated a frequency dependence of the storage (G′) and loss (G”) moduli, with G’ > G’’. The presence of 1% and 2% of pectin in the mixture caused an increase in G’. However, a PEC concentration above 2% led to a decrease in G′ regardless of the pH or frequency. FT-IR spectroscopy indicated that as the PEC content increased, a higher proportion of β-sheet structures was found. Confocal microscopy also confirmed aggregation of ALB particles due to the PEC concentration.
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spelling Rheological and structural characterization of gels from albumin and low methoxyl amidated pectin mixturesBiopolymersConfocal microscopyGelationPolysaccharidesProteinRheologyThe rheological behavior of gels prepared from albumin (ALB) and a mixture of ALB and low methoxyl amidated pectin (PEC) with different proportions were studied using oscillatory rheological tests, Fourier transform infrared spectroscopy (FT-IR spectroscopy), zeta potential measurements and confocal microscopy. The mixture was always 5% of the total solute. Assays were performed in two series, one in which all mixtures were adjusted to pH = 8 and another one without pH adjustment. The zeta potential values indicated the presence of a negative net charge on ALB-PEC gels. The rheological tests indicated a frequency dependence of the storage (G′) and loss (G”) moduli, with G’ > G’’. The presence of 1% and 2% of pectin in the mixture caused an increase in G’. However, a PEC concentration above 2% led to a decrease in G′ regardless of the pH or frequency. FT-IR spectroscopy indicated that as the PEC content increased, a higher proportion of β-sheet structures was found. Confocal microscopy also confirmed aggregation of ALB particles due to the PEC concentration.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Food Engineering and Technology São Paulo State University (Unesp) Institute of Biosciences Humanities and São José do Rio Preto Exact Sciences (IBILCE) São José do Rio Preto, Rua Cristovão Colombo 2265Department of Food Engineering and Technology São Paulo State University (Unesp) Institute of Biosciences Humanities and São José do Rio Preto Exact Sciences (IBILCE) São José do Rio Preto, Rua Cristovão Colombo 2265FAPESP: FAPESP 2017/02808-6Universidade Estadual Paulista (Unesp)Chaux-Gutiérrez, Ana María [UNESP]Pérez-Monterroza, Ezequiel José [UNESP]Mauro, Maria Aparecida [UNESP]2019-10-06T16:15:59Z2019-10-06T16:15:59Z2019-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article60-68http://dx.doi.org/10.1016/j.foodhyd.2019.01.025Food Hydrocolloids, v. 92, p. 60-68.0268-005Xhttp://hdl.handle.net/11449/18868810.1016/j.foodhyd.2019.01.0252-s2.0-850608905392405706828751494Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFood Hydrocolloidsinfo:eu-repo/semantics/openAccess2021-10-23T16:30:35Zoai:repositorio.unesp.br:11449/188688Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:01:46.149350Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Rheological and structural characterization of gels from albumin and low methoxyl amidated pectin mixtures
title Rheological and structural characterization of gels from albumin and low methoxyl amidated pectin mixtures
spellingShingle Rheological and structural characterization of gels from albumin and low methoxyl amidated pectin mixtures
Chaux-Gutiérrez, Ana María [UNESP]
Biopolymers
Confocal microscopy
Gelation
Polysaccharides
Protein
Rheology
title_short Rheological and structural characterization of gels from albumin and low methoxyl amidated pectin mixtures
title_full Rheological and structural characterization of gels from albumin and low methoxyl amidated pectin mixtures
title_fullStr Rheological and structural characterization of gels from albumin and low methoxyl amidated pectin mixtures
title_full_unstemmed Rheological and structural characterization of gels from albumin and low methoxyl amidated pectin mixtures
title_sort Rheological and structural characterization of gels from albumin and low methoxyl amidated pectin mixtures
author Chaux-Gutiérrez, Ana María [UNESP]
author_facet Chaux-Gutiérrez, Ana María [UNESP]
Pérez-Monterroza, Ezequiel José [UNESP]
Mauro, Maria Aparecida [UNESP]
author_role author
author2 Pérez-Monterroza, Ezequiel José [UNESP]
Mauro, Maria Aparecida [UNESP]
author2_role author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Chaux-Gutiérrez, Ana María [UNESP]
Pérez-Monterroza, Ezequiel José [UNESP]
Mauro, Maria Aparecida [UNESP]
dc.subject.por.fl_str_mv Biopolymers
Confocal microscopy
Gelation
Polysaccharides
Protein
Rheology
topic Biopolymers
Confocal microscopy
Gelation
Polysaccharides
Protein
Rheology
description The rheological behavior of gels prepared from albumin (ALB) and a mixture of ALB and low methoxyl amidated pectin (PEC) with different proportions were studied using oscillatory rheological tests, Fourier transform infrared spectroscopy (FT-IR spectroscopy), zeta potential measurements and confocal microscopy. The mixture was always 5% of the total solute. Assays were performed in two series, one in which all mixtures were adjusted to pH = 8 and another one without pH adjustment. The zeta potential values indicated the presence of a negative net charge on ALB-PEC gels. The rheological tests indicated a frequency dependence of the storage (G′) and loss (G”) moduli, with G’ > G’’. The presence of 1% and 2% of pectin in the mixture caused an increase in G’. However, a PEC concentration above 2% led to a decrease in G′ regardless of the pH or frequency. FT-IR spectroscopy indicated that as the PEC content increased, a higher proportion of β-sheet structures was found. Confocal microscopy also confirmed aggregation of ALB particles due to the PEC concentration.
publishDate 2019
dc.date.none.fl_str_mv 2019-10-06T16:15:59Z
2019-10-06T16:15:59Z
2019-07-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.foodhyd.2019.01.025
Food Hydrocolloids, v. 92, p. 60-68.
0268-005X
http://hdl.handle.net/11449/188688
10.1016/j.foodhyd.2019.01.025
2-s2.0-85060890539
2405706828751494
url http://dx.doi.org/10.1016/j.foodhyd.2019.01.025
http://hdl.handle.net/11449/188688
identifier_str_mv Food Hydrocolloids, v. 92, p. 60-68.
0268-005X
10.1016/j.foodhyd.2019.01.025
2-s2.0-85060890539
2405706828751494
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Food Hydrocolloids
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 60-68
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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