Rheological and structural characterization of gels from albumin and low methoxyl amidated pectin mixtures
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.foodhyd.2019.01.025 http://hdl.handle.net/11449/188688 |
Resumo: | The rheological behavior of gels prepared from albumin (ALB) and a mixture of ALB and low methoxyl amidated pectin (PEC) with different proportions were studied using oscillatory rheological tests, Fourier transform infrared spectroscopy (FT-IR spectroscopy), zeta potential measurements and confocal microscopy. The mixture was always 5% of the total solute. Assays were performed in two series, one in which all mixtures were adjusted to pH = 8 and another one without pH adjustment. The zeta potential values indicated the presence of a negative net charge on ALB-PEC gels. The rheological tests indicated a frequency dependence of the storage (G′) and loss (G”) moduli, with G’ > G’’. The presence of 1% and 2% of pectin in the mixture caused an increase in G’. However, a PEC concentration above 2% led to a decrease in G′ regardless of the pH or frequency. FT-IR spectroscopy indicated that as the PEC content increased, a higher proportion of β-sheet structures was found. Confocal microscopy also confirmed aggregation of ALB particles due to the PEC concentration. |
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Rheological and structural characterization of gels from albumin and low methoxyl amidated pectin mixturesBiopolymersConfocal microscopyGelationPolysaccharidesProteinRheologyThe rheological behavior of gels prepared from albumin (ALB) and a mixture of ALB and low methoxyl amidated pectin (PEC) with different proportions were studied using oscillatory rheological tests, Fourier transform infrared spectroscopy (FT-IR spectroscopy), zeta potential measurements and confocal microscopy. The mixture was always 5% of the total solute. Assays were performed in two series, one in which all mixtures were adjusted to pH = 8 and another one without pH adjustment. The zeta potential values indicated the presence of a negative net charge on ALB-PEC gels. The rheological tests indicated a frequency dependence of the storage (G′) and loss (G”) moduli, with G’ > G’’. The presence of 1% and 2% of pectin in the mixture caused an increase in G’. However, a PEC concentration above 2% led to a decrease in G′ regardless of the pH or frequency. FT-IR spectroscopy indicated that as the PEC content increased, a higher proportion of β-sheet structures was found. Confocal microscopy also confirmed aggregation of ALB particles due to the PEC concentration.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Food Engineering and Technology São Paulo State University (Unesp) Institute of Biosciences Humanities and São José do Rio Preto Exact Sciences (IBILCE) São José do Rio Preto, Rua Cristovão Colombo 2265Department of Food Engineering and Technology São Paulo State University (Unesp) Institute of Biosciences Humanities and São José do Rio Preto Exact Sciences (IBILCE) São José do Rio Preto, Rua Cristovão Colombo 2265FAPESP: FAPESP 2017/02808-6Universidade Estadual Paulista (Unesp)Chaux-Gutiérrez, Ana María [UNESP]Pérez-Monterroza, Ezequiel José [UNESP]Mauro, Maria Aparecida [UNESP]2019-10-06T16:15:59Z2019-10-06T16:15:59Z2019-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article60-68http://dx.doi.org/10.1016/j.foodhyd.2019.01.025Food Hydrocolloids, v. 92, p. 60-68.0268-005Xhttp://hdl.handle.net/11449/18868810.1016/j.foodhyd.2019.01.0252-s2.0-850608905392405706828751494Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFood Hydrocolloidsinfo:eu-repo/semantics/openAccess2021-10-23T16:30:35Zoai:repositorio.unesp.br:11449/188688Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:01:46.149350Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Rheological and structural characterization of gels from albumin and low methoxyl amidated pectin mixtures |
title |
Rheological and structural characterization of gels from albumin and low methoxyl amidated pectin mixtures |
spellingShingle |
Rheological and structural characterization of gels from albumin and low methoxyl amidated pectin mixtures Chaux-Gutiérrez, Ana María [UNESP] Biopolymers Confocal microscopy Gelation Polysaccharides Protein Rheology |
title_short |
Rheological and structural characterization of gels from albumin and low methoxyl amidated pectin mixtures |
title_full |
Rheological and structural characterization of gels from albumin and low methoxyl amidated pectin mixtures |
title_fullStr |
Rheological and structural characterization of gels from albumin and low methoxyl amidated pectin mixtures |
title_full_unstemmed |
Rheological and structural characterization of gels from albumin and low methoxyl amidated pectin mixtures |
title_sort |
Rheological and structural characterization of gels from albumin and low methoxyl amidated pectin mixtures |
author |
Chaux-Gutiérrez, Ana María [UNESP] |
author_facet |
Chaux-Gutiérrez, Ana María [UNESP] Pérez-Monterroza, Ezequiel José [UNESP] Mauro, Maria Aparecida [UNESP] |
author_role |
author |
author2 |
Pérez-Monterroza, Ezequiel José [UNESP] Mauro, Maria Aparecida [UNESP] |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Chaux-Gutiérrez, Ana María [UNESP] Pérez-Monterroza, Ezequiel José [UNESP] Mauro, Maria Aparecida [UNESP] |
dc.subject.por.fl_str_mv |
Biopolymers Confocal microscopy Gelation Polysaccharides Protein Rheology |
topic |
Biopolymers Confocal microscopy Gelation Polysaccharides Protein Rheology |
description |
The rheological behavior of gels prepared from albumin (ALB) and a mixture of ALB and low methoxyl amidated pectin (PEC) with different proportions were studied using oscillatory rheological tests, Fourier transform infrared spectroscopy (FT-IR spectroscopy), zeta potential measurements and confocal microscopy. The mixture was always 5% of the total solute. Assays were performed in two series, one in which all mixtures were adjusted to pH = 8 and another one without pH adjustment. The zeta potential values indicated the presence of a negative net charge on ALB-PEC gels. The rheological tests indicated a frequency dependence of the storage (G′) and loss (G”) moduli, with G’ > G’’. The presence of 1% and 2% of pectin in the mixture caused an increase in G’. However, a PEC concentration above 2% led to a decrease in G′ regardless of the pH or frequency. FT-IR spectroscopy indicated that as the PEC content increased, a higher proportion of β-sheet structures was found. Confocal microscopy also confirmed aggregation of ALB particles due to the PEC concentration. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-06T16:15:59Z 2019-10-06T16:15:59Z 2019-07-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.foodhyd.2019.01.025 Food Hydrocolloids, v. 92, p. 60-68. 0268-005X http://hdl.handle.net/11449/188688 10.1016/j.foodhyd.2019.01.025 2-s2.0-85060890539 2405706828751494 |
url |
http://dx.doi.org/10.1016/j.foodhyd.2019.01.025 http://hdl.handle.net/11449/188688 |
identifier_str_mv |
Food Hydrocolloids, v. 92, p. 60-68. 0268-005X 10.1016/j.foodhyd.2019.01.025 2-s2.0-85060890539 2405706828751494 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Food Hydrocolloids |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
60-68 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128741914705920 |