Biosynthesis of silver nanoparticles using Lawsonia inermis and their biomedical application
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1049/nbt2.12096 http://hdl.handle.net/11449/240722 |
Resumo: | Developing biosynthesis of silver nanoparticles (Ag-NPs) using plant extract is an environmentally friendly method to reduce the use of harmful chemical substances. The green synthesis of Ag-NPs by Lawsonia inermis extract and its cellular toxicity and the antimicrobial effect was studied. The physical and chemical properties of synthesised Ag-NPs were investigated using UV-visible spectroscopy, infrared spectroscopy, X-ray diffraction (XRD), scanning, and transmission electron microscopy. The average size of Ag-NPs was 40 nm. The XRD result shows peaks at 2θ = 38.07°, 44.26°, 64.43°, and 77.35° are related to the FCC structure of Ag-NPs. Cytotoxicity of synthesised nanoparticles was evaluated by MTT toxicity test on breast cancer MCF7 cell line. Observations showed that the effect of cytotoxicity of nanoparticles on the studied cell line depended on concentration and time. The obtained IC50 was considered for cells at a dose of 250 μg/ml. Growth and survival rates decreased exponentially with the dose. Antimicrobial properties of Ag-NPs synthesised with extract were investigated against Escherichia coli, Salmonella typhimurium, Bacillus cereus, and Staphylococcus aureus to calculate the minimum inhibitory concentration and the minimum bactericidal concentration of (MBC). The results showed that the synthesised Ag-NPs and the plant extract have antimicrobial properties. The lowest concentration of Ag-NPs that can inhibit the growth of bacterial strains was 25 μg/ml. |
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Biosynthesis of silver nanoparticles using Lawsonia inermis and their biomedical applicationAgNPsantibacterial activityLawsonia inermisUV-visible spectroscopyX-ray diffractionDeveloping biosynthesis of silver nanoparticles (Ag-NPs) using plant extract is an environmentally friendly method to reduce the use of harmful chemical substances. The green synthesis of Ag-NPs by Lawsonia inermis extract and its cellular toxicity and the antimicrobial effect was studied. The physical and chemical properties of synthesised Ag-NPs were investigated using UV-visible spectroscopy, infrared spectroscopy, X-ray diffraction (XRD), scanning, and transmission electron microscopy. The average size of Ag-NPs was 40 nm. The XRD result shows peaks at 2θ = 38.07°, 44.26°, 64.43°, and 77.35° are related to the FCC structure of Ag-NPs. Cytotoxicity of synthesised nanoparticles was evaluated by MTT toxicity test on breast cancer MCF7 cell line. Observations showed that the effect of cytotoxicity of nanoparticles on the studied cell line depended on concentration and time. The obtained IC50 was considered for cells at a dose of 250 μg/ml. Growth and survival rates decreased exponentially with the dose. Antimicrobial properties of Ag-NPs synthesised with extract were investigated against Escherichia coli, Salmonella typhimurium, Bacillus cereus, and Staphylococcus aureus to calculate the minimum inhibitory concentration and the minimum bactericidal concentration of (MBC). The results showed that the synthesised Ag-NPs and the plant extract have antimicrobial properties. The lowest concentration of Ag-NPs that can inhibit the growth of bacterial strains was 25 μg/ml.Department of Biology College of Science Princess Nourah bint Abdulrahman UniversityAl-Maarif University College Medical Laboratory Techniques DepartmentDepartment of Chemistry College of Science Salahaddin University-ErbilDepartment of Medical Biochemical Analysis Cihan University-ErbilSão Paulo State University (Unesp) School of Technology and Sciences, Sao PauloAntibacterial Materials R&D Centre China Metal New Materials (Huzhou) Institute, ZhejiangDepartment of Medical Laboratories Techniques Al-Mustaqbal University CollegeMedical Laboratory Technology Department College of Medical Technology The Islamic UniversityDepartment of Biology College of Education for Pure ScienceSão Paulo State University (Unesp) School of Technology and Sciences, Sao PauloPrincess Nourah bint Abdulrahman UniversityMedical Laboratory Techniques DepartmentSalahaddin University-ErbilCihan University-ErbilUniversidade Estadual Paulista (UNESP)China Metal New Materials (Huzhou) InstituteAl-Mustaqbal University CollegeThe Islamic UniversityCollege of Education for Pure ScienceAlhomaidi, EmanJasim, Saade AbdalkareemAmin, Hawraz Ibrahim M.Nobre, Marcos Augusto Lima [UNESP]Khatami, MehrdadJalil, Abduladheem TurkiHussain Dilfy, Saja2023-03-01T20:29:59Z2023-03-01T20:29:59Z2022-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1049/nbt2.12096IET Nanobiotechnology.1751-875X1751-8741http://hdl.handle.net/11449/24072210.1049/nbt2.120962-s2.0-85136874798Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengIET Nanobiotechnologyinfo:eu-repo/semantics/openAccess2023-03-01T20:29:59Zoai:repositorio.unesp.br:11449/240722Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:54:31.029737Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Biosynthesis of silver nanoparticles using Lawsonia inermis and their biomedical application |
title |
Biosynthesis of silver nanoparticles using Lawsonia inermis and their biomedical application |
spellingShingle |
Biosynthesis of silver nanoparticles using Lawsonia inermis and their biomedical application Alhomaidi, Eman AgNPs antibacterial activity Lawsonia inermis UV-visible spectroscopy X-ray diffraction |
title_short |
Biosynthesis of silver nanoparticles using Lawsonia inermis and their biomedical application |
title_full |
Biosynthesis of silver nanoparticles using Lawsonia inermis and their biomedical application |
title_fullStr |
Biosynthesis of silver nanoparticles using Lawsonia inermis and their biomedical application |
title_full_unstemmed |
Biosynthesis of silver nanoparticles using Lawsonia inermis and their biomedical application |
title_sort |
Biosynthesis of silver nanoparticles using Lawsonia inermis and their biomedical application |
author |
Alhomaidi, Eman |
author_facet |
Alhomaidi, Eman Jasim, Saade Abdalkareem Amin, Hawraz Ibrahim M. Nobre, Marcos Augusto Lima [UNESP] Khatami, Mehrdad Jalil, Abduladheem Turki Hussain Dilfy, Saja |
author_role |
author |
author2 |
Jasim, Saade Abdalkareem Amin, Hawraz Ibrahim M. Nobre, Marcos Augusto Lima [UNESP] Khatami, Mehrdad Jalil, Abduladheem Turki Hussain Dilfy, Saja |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Princess Nourah bint Abdulrahman University Medical Laboratory Techniques Department Salahaddin University-Erbil Cihan University-Erbil Universidade Estadual Paulista (UNESP) China Metal New Materials (Huzhou) Institute Al-Mustaqbal University College The Islamic University College of Education for Pure Science |
dc.contributor.author.fl_str_mv |
Alhomaidi, Eman Jasim, Saade Abdalkareem Amin, Hawraz Ibrahim M. Nobre, Marcos Augusto Lima [UNESP] Khatami, Mehrdad Jalil, Abduladheem Turki Hussain Dilfy, Saja |
dc.subject.por.fl_str_mv |
AgNPs antibacterial activity Lawsonia inermis UV-visible spectroscopy X-ray diffraction |
topic |
AgNPs antibacterial activity Lawsonia inermis UV-visible spectroscopy X-ray diffraction |
description |
Developing biosynthesis of silver nanoparticles (Ag-NPs) using plant extract is an environmentally friendly method to reduce the use of harmful chemical substances. The green synthesis of Ag-NPs by Lawsonia inermis extract and its cellular toxicity and the antimicrobial effect was studied. The physical and chemical properties of synthesised Ag-NPs were investigated using UV-visible spectroscopy, infrared spectroscopy, X-ray diffraction (XRD), scanning, and transmission electron microscopy. The average size of Ag-NPs was 40 nm. The XRD result shows peaks at 2θ = 38.07°, 44.26°, 64.43°, and 77.35° are related to the FCC structure of Ag-NPs. Cytotoxicity of synthesised nanoparticles was evaluated by MTT toxicity test on breast cancer MCF7 cell line. Observations showed that the effect of cytotoxicity of nanoparticles on the studied cell line depended on concentration and time. The obtained IC50 was considered for cells at a dose of 250 μg/ml. Growth and survival rates decreased exponentially with the dose. Antimicrobial properties of Ag-NPs synthesised with extract were investigated against Escherichia coli, Salmonella typhimurium, Bacillus cereus, and Staphylococcus aureus to calculate the minimum inhibitory concentration and the minimum bactericidal concentration of (MBC). The results showed that the synthesised Ag-NPs and the plant extract have antimicrobial properties. The lowest concentration of Ag-NPs that can inhibit the growth of bacterial strains was 25 μg/ml. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-01-01 2023-03-01T20:29:59Z 2023-03-01T20:29:59Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1049/nbt2.12096 IET Nanobiotechnology. 1751-875X 1751-8741 http://hdl.handle.net/11449/240722 10.1049/nbt2.12096 2-s2.0-85136874798 |
url |
http://dx.doi.org/10.1049/nbt2.12096 http://hdl.handle.net/11449/240722 |
identifier_str_mv |
IET Nanobiotechnology. 1751-875X 1751-8741 10.1049/nbt2.12096 2-s2.0-85136874798 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
IET Nanobiotechnology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808128433718296576 |