Biosynthesis of silver nanoparticles using Lawsonia inermis and their biomedical application

Detalhes bibliográficos
Autor(a) principal: Alhomaidi, Eman
Data de Publicação: 2022
Outros Autores: Jasim, Saade Abdalkareem, Amin, Hawraz Ibrahim M., Nobre, Marcos Augusto Lima [UNESP], Khatami, Mehrdad, Jalil, Abduladheem Turki, Hussain Dilfy, Saja
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1049/nbt2.12096
http://hdl.handle.net/11449/240722
Resumo: Developing biosynthesis of silver nanoparticles (Ag-NPs) using plant extract is an environmentally friendly method to reduce the use of harmful chemical substances. The green synthesis of Ag-NPs by Lawsonia inermis extract and its cellular toxicity and the antimicrobial effect was studied. The physical and chemical properties of synthesised Ag-NPs were investigated using UV-visible spectroscopy, infrared spectroscopy, X-ray diffraction (XRD), scanning, and transmission electron microscopy. The average size of Ag-NPs was 40 nm. The XRD result shows peaks at 2θ = 38.07°, 44.26°, 64.43°, and 77.35° are related to the FCC structure of Ag-NPs. Cytotoxicity of synthesised nanoparticles was evaluated by MTT toxicity test on breast cancer MCF7 cell line. Observations showed that the effect of cytotoxicity of nanoparticles on the studied cell line depended on concentration and time. The obtained IC50 was considered for cells at a dose of 250 μg/ml. Growth and survival rates decreased exponentially with the dose. Antimicrobial properties of Ag-NPs synthesised with extract were investigated against Escherichia coli, Salmonella typhimurium, Bacillus cereus, and Staphylococcus aureus to calculate the minimum inhibitory concentration and the minimum bactericidal concentration of (MBC). The results showed that the synthesised Ag-NPs and the plant extract have antimicrobial properties. The lowest concentration of Ag-NPs that can inhibit the growth of bacterial strains was 25 μg/ml.
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spelling Biosynthesis of silver nanoparticles using Lawsonia inermis and their biomedical applicationAgNPsantibacterial activityLawsonia inermisUV-visible spectroscopyX-ray diffractionDeveloping biosynthesis of silver nanoparticles (Ag-NPs) using plant extract is an environmentally friendly method to reduce the use of harmful chemical substances. The green synthesis of Ag-NPs by Lawsonia inermis extract and its cellular toxicity and the antimicrobial effect was studied. The physical and chemical properties of synthesised Ag-NPs were investigated using UV-visible spectroscopy, infrared spectroscopy, X-ray diffraction (XRD), scanning, and transmission electron microscopy. The average size of Ag-NPs was 40 nm. The XRD result shows peaks at 2θ = 38.07°, 44.26°, 64.43°, and 77.35° are related to the FCC structure of Ag-NPs. Cytotoxicity of synthesised nanoparticles was evaluated by MTT toxicity test on breast cancer MCF7 cell line. Observations showed that the effect of cytotoxicity of nanoparticles on the studied cell line depended on concentration and time. The obtained IC50 was considered for cells at a dose of 250 μg/ml. Growth and survival rates decreased exponentially with the dose. Antimicrobial properties of Ag-NPs synthesised with extract were investigated against Escherichia coli, Salmonella typhimurium, Bacillus cereus, and Staphylococcus aureus to calculate the minimum inhibitory concentration and the minimum bactericidal concentration of (MBC). The results showed that the synthesised Ag-NPs and the plant extract have antimicrobial properties. The lowest concentration of Ag-NPs that can inhibit the growth of bacterial strains was 25 μg/ml.Department of Biology College of Science Princess Nourah bint Abdulrahman UniversityAl-Maarif University College Medical Laboratory Techniques DepartmentDepartment of Chemistry College of Science Salahaddin University-ErbilDepartment of Medical Biochemical Analysis Cihan University-ErbilSão Paulo State University (Unesp) School of Technology and Sciences, Sao PauloAntibacterial Materials R&D Centre China Metal New Materials (Huzhou) Institute, ZhejiangDepartment of Medical Laboratories Techniques Al-Mustaqbal University CollegeMedical Laboratory Technology Department College of Medical Technology The Islamic UniversityDepartment of Biology College of Education for Pure ScienceSão Paulo State University (Unesp) School of Technology and Sciences, Sao PauloPrincess Nourah bint Abdulrahman UniversityMedical Laboratory Techniques DepartmentSalahaddin University-ErbilCihan University-ErbilUniversidade Estadual Paulista (UNESP)China Metal New Materials (Huzhou) InstituteAl-Mustaqbal University CollegeThe Islamic UniversityCollege of Education for Pure ScienceAlhomaidi, EmanJasim, Saade AbdalkareemAmin, Hawraz Ibrahim M.Nobre, Marcos Augusto Lima [UNESP]Khatami, MehrdadJalil, Abduladheem TurkiHussain Dilfy, Saja2023-03-01T20:29:59Z2023-03-01T20:29:59Z2022-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1049/nbt2.12096IET Nanobiotechnology.1751-875X1751-8741http://hdl.handle.net/11449/24072210.1049/nbt2.120962-s2.0-85136874798Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengIET Nanobiotechnologyinfo:eu-repo/semantics/openAccess2023-03-01T20:29:59Zoai:repositorio.unesp.br:11449/240722Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T14:54:31.029737Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Biosynthesis of silver nanoparticles using Lawsonia inermis and their biomedical application
title Biosynthesis of silver nanoparticles using Lawsonia inermis and their biomedical application
spellingShingle Biosynthesis of silver nanoparticles using Lawsonia inermis and their biomedical application
Alhomaidi, Eman
AgNPs
antibacterial activity
Lawsonia inermis
UV-visible spectroscopy
X-ray diffraction
title_short Biosynthesis of silver nanoparticles using Lawsonia inermis and their biomedical application
title_full Biosynthesis of silver nanoparticles using Lawsonia inermis and their biomedical application
title_fullStr Biosynthesis of silver nanoparticles using Lawsonia inermis and their biomedical application
title_full_unstemmed Biosynthesis of silver nanoparticles using Lawsonia inermis and their biomedical application
title_sort Biosynthesis of silver nanoparticles using Lawsonia inermis and their biomedical application
author Alhomaidi, Eman
author_facet Alhomaidi, Eman
Jasim, Saade Abdalkareem
Amin, Hawraz Ibrahim M.
Nobre, Marcos Augusto Lima [UNESP]
Khatami, Mehrdad
Jalil, Abduladheem Turki
Hussain Dilfy, Saja
author_role author
author2 Jasim, Saade Abdalkareem
Amin, Hawraz Ibrahim M.
Nobre, Marcos Augusto Lima [UNESP]
Khatami, Mehrdad
Jalil, Abduladheem Turki
Hussain Dilfy, Saja
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Princess Nourah bint Abdulrahman University
Medical Laboratory Techniques Department
Salahaddin University-Erbil
Cihan University-Erbil
Universidade Estadual Paulista (UNESP)
China Metal New Materials (Huzhou) Institute
Al-Mustaqbal University College
The Islamic University
College of Education for Pure Science
dc.contributor.author.fl_str_mv Alhomaidi, Eman
Jasim, Saade Abdalkareem
Amin, Hawraz Ibrahim M.
Nobre, Marcos Augusto Lima [UNESP]
Khatami, Mehrdad
Jalil, Abduladheem Turki
Hussain Dilfy, Saja
dc.subject.por.fl_str_mv AgNPs
antibacterial activity
Lawsonia inermis
UV-visible spectroscopy
X-ray diffraction
topic AgNPs
antibacterial activity
Lawsonia inermis
UV-visible spectroscopy
X-ray diffraction
description Developing biosynthesis of silver nanoparticles (Ag-NPs) using plant extract is an environmentally friendly method to reduce the use of harmful chemical substances. The green synthesis of Ag-NPs by Lawsonia inermis extract and its cellular toxicity and the antimicrobial effect was studied. The physical and chemical properties of synthesised Ag-NPs were investigated using UV-visible spectroscopy, infrared spectroscopy, X-ray diffraction (XRD), scanning, and transmission electron microscopy. The average size of Ag-NPs was 40 nm. The XRD result shows peaks at 2θ = 38.07°, 44.26°, 64.43°, and 77.35° are related to the FCC structure of Ag-NPs. Cytotoxicity of synthesised nanoparticles was evaluated by MTT toxicity test on breast cancer MCF7 cell line. Observations showed that the effect of cytotoxicity of nanoparticles on the studied cell line depended on concentration and time. The obtained IC50 was considered for cells at a dose of 250 μg/ml. Growth and survival rates decreased exponentially with the dose. Antimicrobial properties of Ag-NPs synthesised with extract were investigated against Escherichia coli, Salmonella typhimurium, Bacillus cereus, and Staphylococcus aureus to calculate the minimum inhibitory concentration and the minimum bactericidal concentration of (MBC). The results showed that the synthesised Ag-NPs and the plant extract have antimicrobial properties. The lowest concentration of Ag-NPs that can inhibit the growth of bacterial strains was 25 μg/ml.
publishDate 2022
dc.date.none.fl_str_mv 2022-01-01
2023-03-01T20:29:59Z
2023-03-01T20:29:59Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1049/nbt2.12096
IET Nanobiotechnology.
1751-875X
1751-8741
http://hdl.handle.net/11449/240722
10.1049/nbt2.12096
2-s2.0-85136874798
url http://dx.doi.org/10.1049/nbt2.12096
http://hdl.handle.net/11449/240722
identifier_str_mv IET Nanobiotechnology.
1751-875X
1751-8741
10.1049/nbt2.12096
2-s2.0-85136874798
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv IET Nanobiotechnology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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