Molecular epidemiology and extended-spectrum β-lactamases production of klebsiella pneumoniae isolated from three dairy herds
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S0100-736X2013000700005 http://hdl.handle.net/11449/76672 |
Resumo: | The objectives of this study were to isolate Klebsiella pneumoniae from different sources in three dairy cattle herds, to use the pulsed-field gel electrophoresis (PFGE) to measure genotypic similarities between isolates within a dairy herd, to verify the production of extended- spectrum β-lactamases (ESBLs) by the double-disk synergy test (DDST), and to use the PCR to detect the main ESBLs subgroups genes. Three dairy farms were selected based on previous mastitis outbreaks caused by K. pneumoniae. Milk samples were collected from lactating cows and from the bulk tank. Swabs were performed in different locations, including milking parlors, waiting room, soil, animal's hind limbs and rectum. K. pneumoniae was isolated from 27 cases of intramammary infections (IMI) and from 41 swabs. For farm A isolates from IMI and bulk tank were considered of the same PGFE subtype. One isolate from a bulk tank, three from IMI cases and four from environmental samples were positive in the DDST test. All eight DDST positive isolates harbored the blashv gene, one harbored the blatem gene, and three harbored the blactx-m gene, including the bulk tank isolate. Our study confirms that ESBL producing bacteria is present in different locations in dairy farms, and may be responsible for IMI. The detection of ESBLs on dairy herds could be a major concern for both public and animal health. |
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Molecular epidemiology and extended-spectrum β-lactamases production of klebsiella pneumoniae isolated from three dairy herdsDairy cattle herdsExtended-spectrum β-lactamases (ESBLs)Klebsiella pneumoniaePulsed-field gel electrophoresis (PFGE)The objectives of this study were to isolate Klebsiella pneumoniae from different sources in three dairy cattle herds, to use the pulsed-field gel electrophoresis (PFGE) to measure genotypic similarities between isolates within a dairy herd, to verify the production of extended- spectrum β-lactamases (ESBLs) by the double-disk synergy test (DDST), and to use the PCR to detect the main ESBLs subgroups genes. Three dairy farms were selected based on previous mastitis outbreaks caused by K. pneumoniae. Milk samples were collected from lactating cows and from the bulk tank. Swabs were performed in different locations, including milking parlors, waiting room, soil, animal's hind limbs and rectum. K. pneumoniae was isolated from 27 cases of intramammary infections (IMI) and from 41 swabs. For farm A isolates from IMI and bulk tank were considered of the same PGFE subtype. One isolate from a bulk tank, three from IMI cases and four from environmental samples were positive in the DDST test. All eight DDST positive isolates harbored the blashv gene, one harbored the blatem gene, and three harbored the blactx-m gene, including the bulk tank isolate. Our study confirms that ESBL producing bacteria is present in different locations in dairy farms, and may be responsible for IMI. The detection of ESBLs on dairy herds could be a major concern for both public and animal health.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Departamento de Higiene Veterinária e Saúde Pública Faculdade de Medicina Veterinária e Zootecnia Universidade Estadual Paulista (Unesp), Distrito de Rubião Júnior s/n, Botucatu, SP 18618-970Departamento de Microbiologia e Imunologia Instituto de Biociências, Universidade Estadual Paulista, Distrito de Rubião Júnior s/n, Botucatu, SP 18618-970Agência Paulista de Tecnologia dos Agronegócios (APTA), Av. Rodrigues Alves 40040, Bauru, SP 17034-285Departamento de Higiene Veterinária e Saúde Pública Faculdade de Medicina Veterinária e Zootecnia Universidade Estadual Paulista (Unesp), Distrito de Rubião Júnior s/n, Botucatu, SP 18618-970Departamento de Microbiologia e Imunologia Instituto de Biociências, Universidade Estadual Paulista, Distrito de Rubião Júnior s/n, Botucatu, SP 18618-970Universidade Estadual Paulista (Unesp)Agência Paulista de Tecnologia dos Agronegócios (APTA)Nóbrega, Diego B. [UNESP]Guiduce, Marcos V. S. [UNESP]Guimarães, Felipe F. [UNESP]Riboli, Danilo F. [UNESP]Cunha, Maria L. R. S. [UNESP]Langoni, Hélio [UNESP]Pantoja, José Carlos de Figueiredo [UNESP]Lucheis, Simone Baldini [UNESP]2014-05-27T11:30:45Z2014-05-27T11:30:45Z2013-09-30info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article855-859application/pdfhttp://dx.doi.org/10.1590/S0100-736X2013000700005Pesquisa Veterinaria Brasileira, v. 33, n. 7, p. 855-859, 2013.0100-736X1678-5150http://hdl.handle.net/11449/7667210.1590/S0100-736X2013000700005S0100-736X2013000700005WOS:0003233391000052-s2.0-848845667952-s2.0-84884566795.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPesquisa Veterinária Brasileira0.385info:eu-repo/semantics/openAccess2023-10-26T06:06:26Zoai:repositorio.unesp.br:11449/76672Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-26T06:06:26Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Molecular epidemiology and extended-spectrum β-lactamases production of klebsiella pneumoniae isolated from three dairy herds |
title |
Molecular epidemiology and extended-spectrum β-lactamases production of klebsiella pneumoniae isolated from three dairy herds |
spellingShingle |
Molecular epidemiology and extended-spectrum β-lactamases production of klebsiella pneumoniae isolated from three dairy herds Nóbrega, Diego B. [UNESP] Dairy cattle herds Extended-spectrum β-lactamases (ESBLs) Klebsiella pneumoniae Pulsed-field gel electrophoresis (PFGE) |
title_short |
Molecular epidemiology and extended-spectrum β-lactamases production of klebsiella pneumoniae isolated from three dairy herds |
title_full |
Molecular epidemiology and extended-spectrum β-lactamases production of klebsiella pneumoniae isolated from three dairy herds |
title_fullStr |
Molecular epidemiology and extended-spectrum β-lactamases production of klebsiella pneumoniae isolated from three dairy herds |
title_full_unstemmed |
Molecular epidemiology and extended-spectrum β-lactamases production of klebsiella pneumoniae isolated from three dairy herds |
title_sort |
Molecular epidemiology and extended-spectrum β-lactamases production of klebsiella pneumoniae isolated from three dairy herds |
author |
Nóbrega, Diego B. [UNESP] |
author_facet |
Nóbrega, Diego B. [UNESP] Guiduce, Marcos V. S. [UNESP] Guimarães, Felipe F. [UNESP] Riboli, Danilo F. [UNESP] Cunha, Maria L. R. S. [UNESP] Langoni, Hélio [UNESP] Pantoja, José Carlos de Figueiredo [UNESP] Lucheis, Simone Baldini [UNESP] |
author_role |
author |
author2 |
Guiduce, Marcos V. S. [UNESP] Guimarães, Felipe F. [UNESP] Riboli, Danilo F. [UNESP] Cunha, Maria L. R. S. [UNESP] Langoni, Hélio [UNESP] Pantoja, José Carlos de Figueiredo [UNESP] Lucheis, Simone Baldini [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Agência Paulista de Tecnologia dos Agronegócios (APTA) |
dc.contributor.author.fl_str_mv |
Nóbrega, Diego B. [UNESP] Guiduce, Marcos V. S. [UNESP] Guimarães, Felipe F. [UNESP] Riboli, Danilo F. [UNESP] Cunha, Maria L. R. S. [UNESP] Langoni, Hélio [UNESP] Pantoja, José Carlos de Figueiredo [UNESP] Lucheis, Simone Baldini [UNESP] |
dc.subject.por.fl_str_mv |
Dairy cattle herds Extended-spectrum β-lactamases (ESBLs) Klebsiella pneumoniae Pulsed-field gel electrophoresis (PFGE) |
topic |
Dairy cattle herds Extended-spectrum β-lactamases (ESBLs) Klebsiella pneumoniae Pulsed-field gel electrophoresis (PFGE) |
description |
The objectives of this study were to isolate Klebsiella pneumoniae from different sources in three dairy cattle herds, to use the pulsed-field gel electrophoresis (PFGE) to measure genotypic similarities between isolates within a dairy herd, to verify the production of extended- spectrum β-lactamases (ESBLs) by the double-disk synergy test (DDST), and to use the PCR to detect the main ESBLs subgroups genes. Three dairy farms were selected based on previous mastitis outbreaks caused by K. pneumoniae. Milk samples were collected from lactating cows and from the bulk tank. Swabs were performed in different locations, including milking parlors, waiting room, soil, animal's hind limbs and rectum. K. pneumoniae was isolated from 27 cases of intramammary infections (IMI) and from 41 swabs. For farm A isolates from IMI and bulk tank were considered of the same PGFE subtype. One isolate from a bulk tank, three from IMI cases and four from environmental samples were positive in the DDST test. All eight DDST positive isolates harbored the blashv gene, one harbored the blatem gene, and three harbored the blactx-m gene, including the bulk tank isolate. Our study confirms that ESBL producing bacteria is present in different locations in dairy farms, and may be responsible for IMI. The detection of ESBLs on dairy herds could be a major concern for both public and animal health. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-09-30 2014-05-27T11:30:45Z 2014-05-27T11:30:45Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S0100-736X2013000700005 Pesquisa Veterinaria Brasileira, v. 33, n. 7, p. 855-859, 2013. 0100-736X 1678-5150 http://hdl.handle.net/11449/76672 10.1590/S0100-736X2013000700005 S0100-736X2013000700005 WOS:000323339100005 2-s2.0-84884566795 2-s2.0-84884566795.pdf |
url |
http://dx.doi.org/10.1590/S0100-736X2013000700005 http://hdl.handle.net/11449/76672 |
identifier_str_mv |
Pesquisa Veterinaria Brasileira, v. 33, n. 7, p. 855-859, 2013. 0100-736X 1678-5150 10.1590/S0100-736X2013000700005 S0100-736X2013000700005 WOS:000323339100005 2-s2.0-84884566795 2-s2.0-84884566795.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Pesquisa Veterinária Brasileira 0.385 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
855-859 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964703031558144 |