Voltammetric sensor based on magnetic particles modified composite electrode for determination of triamterene in biological sample

Detalhes bibliográficos
Autor(a) principal: Hudari, Felipe Fantinato [UNESP]
Data de Publicação: 2016
Outros Autores: da Silva, Bianca Ferreira [UNESP], Pividori, Maria Isabel, Zanoni, Maria Valnice Boldrin [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1007/s10008-015-3078-2
http://hdl.handle.net/11449/177599
Resumo: Some diuretic substances are controlled and monitored by the World Anti-Doping Agency as prohibited substances for use by athletes, such as triamterene (TRT). Thus, this work describes a voltammetric method based on graphite-epoxy composite electrode modified by tosyl-functionalized magnetic particles (GECE/MPs-To) for determination of TRT diuretic in urine sample. The TRT presented an oxidation peak at +1.24 V at GECE/MPs-To with irreversible behavior. Controlled potential electrolysis of the TRT at +1.26 V indicated the two electrons are transferred during amine group oxidation and the main product was identified by LC-MS/MS. The anodic peak current is 25 % higher at the modified electrode, suggesting that TRT is adsorbed on the magnetic particles. Using optimized conditions by using multivariate optimization of the parameters inherent of the square wave voltammetry, a calibration curve was constructed with a linear relationship for TRT from 0.500 to 99.8 μmol L−1. The limits of detection and quantification were 1.47 and 4.91 × 10−7 mol L−1, respectively. The proposed method was applied to urine sample and validated by LC-MS/MS technique where the values found and compared between the two techniques showed no significant difference at 95 % confidence.
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spelling Voltammetric sensor based on magnetic particles modified composite electrode for determination of triamterene in biological sampleDiureticGraphite-epoxy composite electrodeMagnetic particlesMultivariate optimizationTriamtereneVoltammetric sensorSome diuretic substances are controlled and monitored by the World Anti-Doping Agency as prohibited substances for use by athletes, such as triamterene (TRT). Thus, this work describes a voltammetric method based on graphite-epoxy composite electrode modified by tosyl-functionalized magnetic particles (GECE/MPs-To) for determination of TRT diuretic in urine sample. The TRT presented an oxidation peak at +1.24 V at GECE/MPs-To with irreversible behavior. Controlled potential electrolysis of the TRT at +1.26 V indicated the two electrons are transferred during amine group oxidation and the main product was identified by LC-MS/MS. The anodic peak current is 25 % higher at the modified electrode, suggesting that TRT is adsorbed on the magnetic particles. Using optimized conditions by using multivariate optimization of the parameters inherent of the square wave voltammetry, a calibration curve was constructed with a linear relationship for TRT from 0.500 to 99.8 μmol L−1. The limits of detection and quantification were 1.47 and 4.91 × 10−7 mol L−1, respectively. The proposed method was applied to urine sample and validated by LC-MS/MS technique where the values found and compared between the two techniques showed no significant difference at 95 % confidence.Departamento de Química UNESP Universidade Estadual Paulista Júlio Mesquita Filho Instituto de Química de Araraquara, Rua Francisco Degni, 55, Bairro Quitandinha,Departamento de Química Grupo de Sensor e Biosensor Universidade Autônoma de Barcelona, BellaterraDepartamento de Química UNESP Universidade Estadual Paulista Júlio Mesquita Filho Instituto de Química de Araraquara, Rua Francisco Degni, 55, Bairro Quitandinha,Universidade Estadual Paulista (Unesp)Universidade Autônoma de BarcelonaHudari, Felipe Fantinato [UNESP]da Silva, Bianca Ferreira [UNESP]Pividori, Maria IsabelZanoni, Maria Valnice Boldrin [UNESP]2018-12-11T17:26:16Z2018-12-11T17:26:16Z2016-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2491-2501application/pdfhttp://dx.doi.org/10.1007/s10008-015-3078-2Journal of Solid State Electrochemistry, v. 20, n. 9, p. 2491-2501, 2016.1432-8488http://hdl.handle.net/11449/17759910.1007/s10008-015-3078-22-s2.0-849474171252-s2.0-84947417125.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Solid State Electrochemistry0,661info:eu-repo/semantics/openAccess2023-10-27T06:10:48Zoai:repositorio.unesp.br:11449/177599Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:10:46.361181Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Voltammetric sensor based on magnetic particles modified composite electrode for determination of triamterene in biological sample
title Voltammetric sensor based on magnetic particles modified composite electrode for determination of triamterene in biological sample
spellingShingle Voltammetric sensor based on magnetic particles modified composite electrode for determination of triamterene in biological sample
Hudari, Felipe Fantinato [UNESP]
Diuretic
Graphite-epoxy composite electrode
Magnetic particles
Multivariate optimization
Triamterene
Voltammetric sensor
title_short Voltammetric sensor based on magnetic particles modified composite electrode for determination of triamterene in biological sample
title_full Voltammetric sensor based on magnetic particles modified composite electrode for determination of triamterene in biological sample
title_fullStr Voltammetric sensor based on magnetic particles modified composite electrode for determination of triamterene in biological sample
title_full_unstemmed Voltammetric sensor based on magnetic particles modified composite electrode for determination of triamterene in biological sample
title_sort Voltammetric sensor based on magnetic particles modified composite electrode for determination of triamterene in biological sample
author Hudari, Felipe Fantinato [UNESP]
author_facet Hudari, Felipe Fantinato [UNESP]
da Silva, Bianca Ferreira [UNESP]
Pividori, Maria Isabel
Zanoni, Maria Valnice Boldrin [UNESP]
author_role author
author2 da Silva, Bianca Ferreira [UNESP]
Pividori, Maria Isabel
Zanoni, Maria Valnice Boldrin [UNESP]
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidade Autônoma de Barcelona
dc.contributor.author.fl_str_mv Hudari, Felipe Fantinato [UNESP]
da Silva, Bianca Ferreira [UNESP]
Pividori, Maria Isabel
Zanoni, Maria Valnice Boldrin [UNESP]
dc.subject.por.fl_str_mv Diuretic
Graphite-epoxy composite electrode
Magnetic particles
Multivariate optimization
Triamterene
Voltammetric sensor
topic Diuretic
Graphite-epoxy composite electrode
Magnetic particles
Multivariate optimization
Triamterene
Voltammetric sensor
description Some diuretic substances are controlled and monitored by the World Anti-Doping Agency as prohibited substances for use by athletes, such as triamterene (TRT). Thus, this work describes a voltammetric method based on graphite-epoxy composite electrode modified by tosyl-functionalized magnetic particles (GECE/MPs-To) for determination of TRT diuretic in urine sample. The TRT presented an oxidation peak at +1.24 V at GECE/MPs-To with irreversible behavior. Controlled potential electrolysis of the TRT at +1.26 V indicated the two electrons are transferred during amine group oxidation and the main product was identified by LC-MS/MS. The anodic peak current is 25 % higher at the modified electrode, suggesting that TRT is adsorbed on the magnetic particles. Using optimized conditions by using multivariate optimization of the parameters inherent of the square wave voltammetry, a calibration curve was constructed with a linear relationship for TRT from 0.500 to 99.8 μmol L−1. The limits of detection and quantification were 1.47 and 4.91 × 10−7 mol L−1, respectively. The proposed method was applied to urine sample and validated by LC-MS/MS technique where the values found and compared between the two techniques showed no significant difference at 95 % confidence.
publishDate 2016
dc.date.none.fl_str_mv 2016-09-01
2018-12-11T17:26:16Z
2018-12-11T17:26:16Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1007/s10008-015-3078-2
Journal of Solid State Electrochemistry, v. 20, n. 9, p. 2491-2501, 2016.
1432-8488
http://hdl.handle.net/11449/177599
10.1007/s10008-015-3078-2
2-s2.0-84947417125
2-s2.0-84947417125.pdf
url http://dx.doi.org/10.1007/s10008-015-3078-2
http://hdl.handle.net/11449/177599
identifier_str_mv Journal of Solid State Electrochemistry, v. 20, n. 9, p. 2491-2501, 2016.
1432-8488
10.1007/s10008-015-3078-2
2-s2.0-84947417125
2-s2.0-84947417125.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of Solid State Electrochemistry
0,661
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 2491-2501
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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