The effect of a Lactobacillus-based probiotic for the control of necrotic enteritis in broilers
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.4236/fns.2013.411A001 http://hdl.handle.net/11449/137038 |
Resumo: | Necrotic Enteritis (NE) caused by Clostridium perfringens (CP) in poultry is probably the most important bacterial disease in terms of economic implications. The disease is multi-factorial and is invariably associated with predisposing factors. The present study investigated the effect of a commercially available Lactobacillus-based probiotic (FM-B11) for the control of necrotic enteritis in broiler chickens. In experiment 1, one-day-of-hatch broiler chicks were randomly allocated to the following treatment groups: 1) Non-challenged (NC); 2) Challenged (C); 3) Challenged + probiotic (C+ FM-B11). Prior to placement, chicks in groups 2 and 3 received 0.25 mL of Salmonella typhimurium (ST) containing 105 cfu of viable cells by oral gavage. At 14, 15 and 16 days of age, all chicks in group 3 were treated with FM-B11 in the drinking water at a concentration of 106 cfu/ml. At 21d of age, all chicks in groups 2 and 3, were individually challenged with 5 × 104 sporulated oocysts of E. maxima by oral gavage. At 26d of age, all chicks in groups 2 and 3, were individually challenged with 108 cfu CP; body weight (BW) was recorded prior to challenge. The experiment was terminated at 29 days of age and the following parameters were evaluated: NE-associated mortality, CP lesion scores, CP concentrations in ileum, BW, and body weight gain (BWG). Chicks treated with FM-B11 had significantly (P < 0.05) higher body weight gain after challenge when compared to control challenge chickens. Total mortality was higher in the C group (48.8%) when compared to the C + FM-B11 (12.7%). Even though there was no significant (P > 0.05) difference in lesion score between C and C + FM-B11, group C + FM-B11 had significantly (P < 0.05) lower total number of cfu of CP recovered from the ileal mucosa and content samples when compared to group C. Experiment 2 was a unique and remarkable case report of a field outbreak of NE in a commercial broiler farm in Argentina. A reduction and control of the mortality associated with NE following 3 days of administration of FM-B11 was observed as compared with the control non treated house. These results imply that the commercially available Lactobacillus-based probiotic FM-B11 was able to reduce the severities of NE, as a secondary bacterial infection, in an experimental NE challenge model; as well as, in a commercial field outbreak of NE. |
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The effect of a Lactobacillus-based probiotic for the control of necrotic enteritis in broilersLactobacillusNecrotic enteritisSalmonellaCoccidiosisProbioticNecrotic Enteritis (NE) caused by Clostridium perfringens (CP) in poultry is probably the most important bacterial disease in terms of economic implications. The disease is multi-factorial and is invariably associated with predisposing factors. The present study investigated the effect of a commercially available Lactobacillus-based probiotic (FM-B11) for the control of necrotic enteritis in broiler chickens. In experiment 1, one-day-of-hatch broiler chicks were randomly allocated to the following treatment groups: 1) Non-challenged (NC); 2) Challenged (C); 3) Challenged + probiotic (C+ FM-B11). Prior to placement, chicks in groups 2 and 3 received 0.25 mL of Salmonella typhimurium (ST) containing 105 cfu of viable cells by oral gavage. At 14, 15 and 16 days of age, all chicks in group 3 were treated with FM-B11 in the drinking water at a concentration of 106 cfu/ml. At 21d of age, all chicks in groups 2 and 3, were individually challenged with 5 × 104 sporulated oocysts of E. maxima by oral gavage. At 26d of age, all chicks in groups 2 and 3, were individually challenged with 108 cfu CP; body weight (BW) was recorded prior to challenge. The experiment was terminated at 29 days of age and the following parameters were evaluated: NE-associated mortality, CP lesion scores, CP concentrations in ileum, BW, and body weight gain (BWG). Chicks treated with FM-B11 had significantly (P < 0.05) higher body weight gain after challenge when compared to control challenge chickens. Total mortality was higher in the C group (48.8%) when compared to the C + FM-B11 (12.7%). Even though there was no significant (P > 0.05) difference in lesion score between C and C + FM-B11, group C + FM-B11 had significantly (P < 0.05) lower total number of cfu of CP recovered from the ileal mucosa and content samples when compared to group C. Experiment 2 was a unique and remarkable case report of a field outbreak of NE in a commercial broiler farm in Argentina. A reduction and control of the mortality associated with NE following 3 days of administration of FM-B11 was observed as compared with the control non treated house. These results imply that the commercially available Lactobacillus-based probiotic FM-B11 was able to reduce the severities of NE, as a secondary bacterial infection, in an experimental NE challenge model; as well as, in a commercial field outbreak of NE.Programa de Apoyo a la Superación del Personal Académico (PASPA)University of Arkansas, Department of Poultry ScienceUniversidad Nacional Autónoma de México, Departamento de Medicina y Zootecnia de Aves, Facultad de Medicina Veterinaria y ZootecniaUniversidade Estadual Paulista, Departamento de Clínica Veterinária, Faculdade de Medicina Veterinária e Zootecnia de BotucatuUniversity of ArkansasUniversidad Nacional Autónoma de MéxicoUniversidade Estadual Paulista (Unesp)Layton, Sherryll LynnHernandez-Velasco, XochitChaitanya, ShivaramaiahXavier, JorgeMenconi, AnitaLatorre, Juan DavidKallapura, GopalaKuttappan, Vivek AyamchirakkunnelWolfenden, Ross ElderonAndreatti Filho, Raphael Lucio [UNESP]Hargis, Billy MarshalTellez, Guillermo2016-04-01T18:43:53Z2016-04-01T18:43:53Z2013info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1-7application/pdfhttp://dx.doi.org/10.4236/fns.2013.411A001Food and Nutrition Sciences, v. 4, n. 11, p. 1-7, 2013.2157-9458http://hdl.handle.net/11449/13703810.4236/fns.2013.411A001ISSN2157-9458-2013-04-11-01-07.pdf8502462873517464Currículo Lattesreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengFood and Nutrition Sciencesinfo:eu-repo/semantics/openAccess2023-12-14T06:21:13Zoai:repositorio.unesp.br:11449/137038Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:19:22.335701Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
The effect of a Lactobacillus-based probiotic for the control of necrotic enteritis in broilers |
title |
The effect of a Lactobacillus-based probiotic for the control of necrotic enteritis in broilers |
spellingShingle |
The effect of a Lactobacillus-based probiotic for the control of necrotic enteritis in broilers Layton, Sherryll Lynn Lactobacillus Necrotic enteritis Salmonella Coccidiosis Probiotic |
title_short |
The effect of a Lactobacillus-based probiotic for the control of necrotic enteritis in broilers |
title_full |
The effect of a Lactobacillus-based probiotic for the control of necrotic enteritis in broilers |
title_fullStr |
The effect of a Lactobacillus-based probiotic for the control of necrotic enteritis in broilers |
title_full_unstemmed |
The effect of a Lactobacillus-based probiotic for the control of necrotic enteritis in broilers |
title_sort |
The effect of a Lactobacillus-based probiotic for the control of necrotic enteritis in broilers |
author |
Layton, Sherryll Lynn |
author_facet |
Layton, Sherryll Lynn Hernandez-Velasco, Xochit Chaitanya, Shivaramaiah Xavier, Jorge Menconi, Anita Latorre, Juan David Kallapura, Gopala Kuttappan, Vivek Ayamchirakkunnel Wolfenden, Ross Elderon Andreatti Filho, Raphael Lucio [UNESP] Hargis, Billy Marshal Tellez, Guillermo |
author_role |
author |
author2 |
Hernandez-Velasco, Xochit Chaitanya, Shivaramaiah Xavier, Jorge Menconi, Anita Latorre, Juan David Kallapura, Gopala Kuttappan, Vivek Ayamchirakkunnel Wolfenden, Ross Elderon Andreatti Filho, Raphael Lucio [UNESP] Hargis, Billy Marshal Tellez, Guillermo |
author2_role |
author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
University of Arkansas Universidad Nacional Autónoma de México Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Layton, Sherryll Lynn Hernandez-Velasco, Xochit Chaitanya, Shivaramaiah Xavier, Jorge Menconi, Anita Latorre, Juan David Kallapura, Gopala Kuttappan, Vivek Ayamchirakkunnel Wolfenden, Ross Elderon Andreatti Filho, Raphael Lucio [UNESP] Hargis, Billy Marshal Tellez, Guillermo |
dc.subject.por.fl_str_mv |
Lactobacillus Necrotic enteritis Salmonella Coccidiosis Probiotic |
topic |
Lactobacillus Necrotic enteritis Salmonella Coccidiosis Probiotic |
description |
Necrotic Enteritis (NE) caused by Clostridium perfringens (CP) in poultry is probably the most important bacterial disease in terms of economic implications. The disease is multi-factorial and is invariably associated with predisposing factors. The present study investigated the effect of a commercially available Lactobacillus-based probiotic (FM-B11) for the control of necrotic enteritis in broiler chickens. In experiment 1, one-day-of-hatch broiler chicks were randomly allocated to the following treatment groups: 1) Non-challenged (NC); 2) Challenged (C); 3) Challenged + probiotic (C+ FM-B11). Prior to placement, chicks in groups 2 and 3 received 0.25 mL of Salmonella typhimurium (ST) containing 105 cfu of viable cells by oral gavage. At 14, 15 and 16 days of age, all chicks in group 3 were treated with FM-B11 in the drinking water at a concentration of 106 cfu/ml. At 21d of age, all chicks in groups 2 and 3, were individually challenged with 5 × 104 sporulated oocysts of E. maxima by oral gavage. At 26d of age, all chicks in groups 2 and 3, were individually challenged with 108 cfu CP; body weight (BW) was recorded prior to challenge. The experiment was terminated at 29 days of age and the following parameters were evaluated: NE-associated mortality, CP lesion scores, CP concentrations in ileum, BW, and body weight gain (BWG). Chicks treated with FM-B11 had significantly (P < 0.05) higher body weight gain after challenge when compared to control challenge chickens. Total mortality was higher in the C group (48.8%) when compared to the C + FM-B11 (12.7%). Even though there was no significant (P > 0.05) difference in lesion score between C and C + FM-B11, group C + FM-B11 had significantly (P < 0.05) lower total number of cfu of CP recovered from the ileal mucosa and content samples when compared to group C. Experiment 2 was a unique and remarkable case report of a field outbreak of NE in a commercial broiler farm in Argentina. A reduction and control of the mortality associated with NE following 3 days of administration of FM-B11 was observed as compared with the control non treated house. These results imply that the commercially available Lactobacillus-based probiotic FM-B11 was able to reduce the severities of NE, as a secondary bacterial infection, in an experimental NE challenge model; as well as, in a commercial field outbreak of NE. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013 2016-04-01T18:43:53Z 2016-04-01T18:43:53Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.4236/fns.2013.411A001 Food and Nutrition Sciences, v. 4, n. 11, p. 1-7, 2013. 2157-9458 http://hdl.handle.net/11449/137038 10.4236/fns.2013.411A001 ISSN2157-9458-2013-04-11-01-07.pdf 8502462873517464 |
url |
http://dx.doi.org/10.4236/fns.2013.411A001 http://hdl.handle.net/11449/137038 |
identifier_str_mv |
Food and Nutrition Sciences, v. 4, n. 11, p. 1-7, 2013. 2157-9458 10.4236/fns.2013.411A001 ISSN2157-9458-2013-04-11-01-07.pdf 8502462873517464 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Food and Nutrition Sciences |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1-7 application/pdf |
dc.source.none.fl_str_mv |
Currículo Lattes reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808129188349083648 |