Análise do transcriptoma da interação Xanthomonas fuscans subsp. aurantifolii tipo C e plantas cítricas

Detalhes bibliográficos
Autor(a) principal: Silva, Claudênia Ferreira da [UNESP]
Data de Publicação: 2015
Tipo de documento: Tese
Idioma: por
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://hdl.handle.net/11449/144058
Resumo: The bacterium Xanthomonas fuscans subsp. aurantifolii type C (XAC) is the causative agent of cancrose C, which affects only acid lime 'Galego' (Citrus aurantifolia (Christm) Swingle). The main phenotypic characteristic of this disease is the hyperplasia and hypertrophy of the affected tissue, leading to the formation of erupescentes characteristic lesions, and responsible for the abnormal cell growth is a transcription activator-like (TAL) effector family (also called the avrBs3/pthA family), which is injected into the plant by the type type III secretion system (T3SS) and is translocated to the nucleus, where it interacts with UPA-box regions (up-regulated by AvrBs3) and acts as a transcriptional activator. The sweet orange varieties are resistant, and when inoculated with XauC show strong hypersensitive response (HR), causing local programmed cell death (apoptosis) and abscission of affected leaves. The mechanisms that give the acid lime 'Galego' susceptibility to XauC is not known. This study aimed to identify, through next-generation sequencing (NGS), plant differentially expressed transcripts involved in host-pathogen interaction in pathosystems XauC-orange 'Hamlin' (species resistant) and XauC-acid lime 'Galego' (species susceptible). Leaves from 'Hamlin' and acid lime 'Galego' were inoculated with XauC suspension at a concentration of 106 cfu/mL (experimental) or sterile water (control) and collected at 5 and 7 days after inoculation (dai). Total RNA was extracted and used for transcriptome analysis by RNA-seq. The transcripts obtained from sequencing were normalized and redundancies removed. A single bank of genomic data and citrus transcripts was assembled, which was called CitSeqDB and is the junction of 6 public banks: AFFYM, NCBI Unigene, USDA, CITRUSGDB and PHYTOZOME. Transcripts that proved to be differentially expressed relative to controls were confronted with the bank and functional annotation was performed using ...
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spelling Análise do transcriptoma da interação Xanthomonas fuscans subsp. aurantifolii tipo C e plantas cítricasCítricosFrutas citricas - Doenças e pragasXanthomonasBacterias fitopatogenicasGenetica vegetalCitrusThe bacterium Xanthomonas fuscans subsp. aurantifolii type C (XAC) is the causative agent of cancrose C, which affects only acid lime 'Galego' (Citrus aurantifolia (Christm) Swingle). The main phenotypic characteristic of this disease is the hyperplasia and hypertrophy of the affected tissue, leading to the formation of erupescentes characteristic lesions, and responsible for the abnormal cell growth is a transcription activator-like (TAL) effector family (also called the avrBs3/pthA family), which is injected into the plant by the type type III secretion system (T3SS) and is translocated to the nucleus, where it interacts with UPA-box regions (up-regulated by AvrBs3) and acts as a transcriptional activator. The sweet orange varieties are resistant, and when inoculated with XauC show strong hypersensitive response (HR), causing local programmed cell death (apoptosis) and abscission of affected leaves. The mechanisms that give the acid lime 'Galego' susceptibility to XauC is not known. This study aimed to identify, through next-generation sequencing (NGS), plant differentially expressed transcripts involved in host-pathogen interaction in pathosystems XauC-orange 'Hamlin' (species resistant) and XauC-acid lime 'Galego' (species susceptible). Leaves from 'Hamlin' and acid lime 'Galego' were inoculated with XauC suspension at a concentration of 106 cfu/mL (experimental) or sterile water (control) and collected at 5 and 7 days after inoculation (dai). Total RNA was extracted and used for transcriptome analysis by RNA-seq. The transcripts obtained from sequencing were normalized and redundancies removed. A single bank of genomic data and citrus transcripts was assembled, which was called CitSeqDB and is the junction of 6 public banks: AFFYM, NCBI Unigene, USDA, CITRUSGDB and PHYTOZOME. Transcripts that proved to be differentially expressed relative to controls were confronted with the bank and functional annotation was performed using ...A bactéria Xanthomonas fuscans subsp. aurantifolii tipo C (XauC) é o agente causal da cancrose C, que afeta apenas lima ácida 'Galego' (Citrus aurantifolia (Christm) Swingle). A principal característica fenotípica desta doença é a hiperplasia e hipertrofia do tecido afetado, que leva à formação de lesões erupescentes características, sendo que o responsável pela proliferação celular desordenada é um efetor tipo TAL da família AvrBs3/PthA, que é injetado na planta pelo sistema de secreção tipo III (SST3) da bactéria e é translocado até o núcleo, onde interage com regiões UPA-box (up-regulated by AvrBs3) e funciona como um ativador transcricional. As variedades de laranja doce são resistentes à cancrose C e, quando inoculadas com XauC apresentam forte reação de hipersensibilidade (HR), causando morte celular programada local (apoptose) e abscisão das folhas afetadas. O(s) mecanismo(s) que confer(em) à lima ácida 'Galego' susceptibilidade à XauC não é(são) conhecido(s). Este estudo teve como objetivo identificar, através de sequenciamento de nova geração (NGS), transcritos de plantas diferencialmente expressos envolvidos na interação patógeno-hospedeiro nos patossistemas XauC-laranja 'Hamlin' (espécie resistente) e XauC-lima ácida 'Galego' (espécie susceptível). Para isso, folhas de laranja 'Hamlin' e lima ácida 'Galego' foram inoculadas com suspensão de XauC a uma concentração de 106 ufc/mL, sendo que o controle foram folhas inoculadas com água estéril. Essas folhas foram coletadas nos tempos de 5 e 7 dias após inoculação (dai) e o RNA total foi extraído e utilizado para a análise dos transcrissomas por RNA-seq. Os transcritos obtidos do sequenciamento foram normalizados e as redundâncias foram removidas. Foi montado um banco único de dados genômicos e de transcritos de citros...Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Capes)Universidade Estadual Paulista (Unesp)Ferro, Jesus Aparecido [UNESP]Belasque Júnior, José [UNESP]Universidade Estadual Paulista (Unesp)Silva, Claudênia Ferreira da [UNESP]2016-09-27T13:40:01Z2016-09-27T13:40:01Z2015-12-07info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisv, 89 p. : il.application/pdfSILVA, Claudênia Ferreira da. Análise do transcriptoma da interação Xanthomonas fuscans subsp. aurantifolii tipo C e plantas cítricas. 2015. v, 89 p. Tese (doutorado) - Universidade Estadual Paulista Júlio de Mesquita Filho, Faculdade de Ciências Agrárias e Veterinárias de Jaboticabal, 2015.http://hdl.handle.net/11449/144058000867476000867476.pdf33004102070P60147241723612464Alephreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporinfo:eu-repo/semantics/openAccess2024-06-05T18:49:00Zoai:repositorio.unesp.br:11449/144058Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:25:44.013499Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Análise do transcriptoma da interação Xanthomonas fuscans subsp. aurantifolii tipo C e plantas cítricas
title Análise do transcriptoma da interação Xanthomonas fuscans subsp. aurantifolii tipo C e plantas cítricas
spellingShingle Análise do transcriptoma da interação Xanthomonas fuscans subsp. aurantifolii tipo C e plantas cítricas
Silva, Claudênia Ferreira da [UNESP]
Cítricos
Frutas citricas - Doenças e pragas
Xanthomonas
Bacterias fitopatogenicas
Genetica vegetal
Citrus
title_short Análise do transcriptoma da interação Xanthomonas fuscans subsp. aurantifolii tipo C e plantas cítricas
title_full Análise do transcriptoma da interação Xanthomonas fuscans subsp. aurantifolii tipo C e plantas cítricas
title_fullStr Análise do transcriptoma da interação Xanthomonas fuscans subsp. aurantifolii tipo C e plantas cítricas
title_full_unstemmed Análise do transcriptoma da interação Xanthomonas fuscans subsp. aurantifolii tipo C e plantas cítricas
title_sort Análise do transcriptoma da interação Xanthomonas fuscans subsp. aurantifolii tipo C e plantas cítricas
author Silva, Claudênia Ferreira da [UNESP]
author_facet Silva, Claudênia Ferreira da [UNESP]
author_role author
dc.contributor.none.fl_str_mv Ferro, Jesus Aparecido [UNESP]
Belasque Júnior, José [UNESP]
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Silva, Claudênia Ferreira da [UNESP]
dc.subject.por.fl_str_mv Cítricos
Frutas citricas - Doenças e pragas
Xanthomonas
Bacterias fitopatogenicas
Genetica vegetal
Citrus
topic Cítricos
Frutas citricas - Doenças e pragas
Xanthomonas
Bacterias fitopatogenicas
Genetica vegetal
Citrus
description The bacterium Xanthomonas fuscans subsp. aurantifolii type C (XAC) is the causative agent of cancrose C, which affects only acid lime 'Galego' (Citrus aurantifolia (Christm) Swingle). The main phenotypic characteristic of this disease is the hyperplasia and hypertrophy of the affected tissue, leading to the formation of erupescentes characteristic lesions, and responsible for the abnormal cell growth is a transcription activator-like (TAL) effector family (also called the avrBs3/pthA family), which is injected into the plant by the type type III secretion system (T3SS) and is translocated to the nucleus, where it interacts with UPA-box regions (up-regulated by AvrBs3) and acts as a transcriptional activator. The sweet orange varieties are resistant, and when inoculated with XauC show strong hypersensitive response (HR), causing local programmed cell death (apoptosis) and abscission of affected leaves. The mechanisms that give the acid lime 'Galego' susceptibility to XauC is not known. This study aimed to identify, through next-generation sequencing (NGS), plant differentially expressed transcripts involved in host-pathogen interaction in pathosystems XauC-orange 'Hamlin' (species resistant) and XauC-acid lime 'Galego' (species susceptible). Leaves from 'Hamlin' and acid lime 'Galego' were inoculated with XauC suspension at a concentration of 106 cfu/mL (experimental) or sterile water (control) and collected at 5 and 7 days after inoculation (dai). Total RNA was extracted and used for transcriptome analysis by RNA-seq. The transcripts obtained from sequencing were normalized and redundancies removed. A single bank of genomic data and citrus transcripts was assembled, which was called CitSeqDB and is the junction of 6 public banks: AFFYM, NCBI Unigene, USDA, CITRUSGDB and PHYTOZOME. Transcripts that proved to be differentially expressed relative to controls were confronted with the bank and functional annotation was performed using ...
publishDate 2015
dc.date.none.fl_str_mv 2015-12-07
2016-09-27T13:40:01Z
2016-09-27T13:40:01Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
status_str publishedVersion
dc.identifier.uri.fl_str_mv SILVA, Claudênia Ferreira da. Análise do transcriptoma da interação Xanthomonas fuscans subsp. aurantifolii tipo C e plantas cítricas. 2015. v, 89 p. Tese (doutorado) - Universidade Estadual Paulista Júlio de Mesquita Filho, Faculdade de Ciências Agrárias e Veterinárias de Jaboticabal, 2015.
http://hdl.handle.net/11449/144058
000867476
000867476.pdf
33004102070P6
0147241723612464
identifier_str_mv SILVA, Claudênia Ferreira da. Análise do transcriptoma da interação Xanthomonas fuscans subsp. aurantifolii tipo C e plantas cítricas. 2015. v, 89 p. Tese (doutorado) - Universidade Estadual Paulista Júlio de Mesquita Filho, Faculdade de Ciências Agrárias e Veterinárias de Jaboticabal, 2015.
000867476
000867476.pdf
33004102070P6
0147241723612464
url http://hdl.handle.net/11449/144058
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.format.none.fl_str_mv v, 89 p. : il.
application/pdf
dc.publisher.none.fl_str_mv Universidade Estadual Paulista (Unesp)
publisher.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.source.none.fl_str_mv Aleph
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
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