Mating mediates morphophysiological changes in the spermathecae of Coptotermes gestroi queens
Autor(a) principal: | |
---|---|
Data de Publicação: | 2023 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1111/eea.13253 http://hdl.handle.net/11449/247850 |
Resumo: | Insect spermathecae play a crucial role in sperm storage and maintenance prior to egg fertilization. Within eusocial insects, this structure is well studied in the Hymenoptera, whose queens copulate during a short period early in life and store sperm for up to decades. Within Isoptera, sperm storage and maintenance inside the spermatheca are poorly understood, especially due to the presence of a sperm-providing king. Here, we compared the morphometric and morphophysiological features of the spermathecae of virgin and mated queens of the invasive termite Coptotermes gestroi (Wasmann) (Isoptera: Rhinotermitidae). The spermatheca comprises a finger-shaped reservoir divided into two regions and a duct limited by a narrow transition. The superficial spermatheca area, as well as the luminal area, increase significantly after insemination, even among queens whose reproductive activity was reduced, suggesting that sperm storage continues during such conditions. The secretion of proteins and polysaccharides into the spermathecal lumen was a remarkable feature for both virgin and 4-year-old queens, although the concentration of the secreted content increased in the latter group. It suggests that spermatheca activation occurs before pairing, but its secretory activity intensifies to nourish and provide energy for the stored spermatozoa. Ultrastructure of the spermathecal epithelium showed a bicellular unit, composed of a secretory cell and associated canal cells. Secretory vesicles of various electron densities were observed next to the receiving canal of the secretory cells in both virgin and 2-year-old queens. Nevertheless, strongly electron-dense vesicles were only recorded for mated queens, which were associated with the increasing synthesis of proteins. The occurrence of rough endoplasmic reticulum and richness of mitochondria reinforces the protein synthesis and transport of contents towards the spermathecal lumen. In conclusion, the spermatheca of C. gestroi undergoes morphometric and physiological changes after mating, and further analysis may provide insights into the chemical nature of the spermathecal secretion prior to and after this event. |
id |
UNSP_ca087dc313bd46d29110f68ed85e78a0 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/247850 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Mating mediates morphophysiological changes in the spermathecae of Coptotermes gestroi queensBlattariaCoptotermes gestroieusocial insecthistochemistryIsopterapest speciesreproductive systemRhinotermitidaesperm storagespermathecatermiteultrastructureInsect spermathecae play a crucial role in sperm storage and maintenance prior to egg fertilization. Within eusocial insects, this structure is well studied in the Hymenoptera, whose queens copulate during a short period early in life and store sperm for up to decades. Within Isoptera, sperm storage and maintenance inside the spermatheca are poorly understood, especially due to the presence of a sperm-providing king. Here, we compared the morphometric and morphophysiological features of the spermathecae of virgin and mated queens of the invasive termite Coptotermes gestroi (Wasmann) (Isoptera: Rhinotermitidae). The spermatheca comprises a finger-shaped reservoir divided into two regions and a duct limited by a narrow transition. The superficial spermatheca area, as well as the luminal area, increase significantly after insemination, even among queens whose reproductive activity was reduced, suggesting that sperm storage continues during such conditions. The secretion of proteins and polysaccharides into the spermathecal lumen was a remarkable feature for both virgin and 4-year-old queens, although the concentration of the secreted content increased in the latter group. It suggests that spermatheca activation occurs before pairing, but its secretory activity intensifies to nourish and provide energy for the stored spermatozoa. Ultrastructure of the spermathecal epithelium showed a bicellular unit, composed of a secretory cell and associated canal cells. Secretory vesicles of various electron densities were observed next to the receiving canal of the secretory cells in both virgin and 2-year-old queens. Nevertheless, strongly electron-dense vesicles were only recorded for mated queens, which were associated with the increasing synthesis of proteins. The occurrence of rough endoplasmic reticulum and richness of mitochondria reinforces the protein synthesis and transport of contents towards the spermathecal lumen. In conclusion, the spermatheca of C. gestroi undergoes morphometric and physiological changes after mating, and further analysis may provide insights into the chemical nature of the spermathecal secretion prior to and after this event.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Laboratório de Cupins Departamento de Biologia Geral e Aplicada Instituto de Biociências de Rio Claro Universidade Estadual Paulista (Unesp), SPLaboratório de Cupins Departamento de Biologia Geral e Aplicada Instituto de Biociências de Rio Claro Universidade Estadual Paulista (Unesp), SPCAPES: 001CNPq: 305539/2014-0Universidade Estadual Paulista (UNESP)da Silva, Iago Bueno [UNESP]Costa-Leonardo, Ana Maria [UNESP]2023-07-29T13:27:37Z2023-07-29T13:27:37Z2023-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article361-373http://dx.doi.org/10.1111/eea.13253Entomologia Experimentalis et Applicata, v. 171, n. 5, p. 361-373, 2023.1570-74580013-8703http://hdl.handle.net/11449/24785010.1111/eea.132532-s2.0-85141538510Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengEntomologia Experimentalis et Applicatainfo:eu-repo/semantics/openAccess2023-07-29T13:27:37Zoai:repositorio.unesp.br:11449/247850Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:09:07.687261Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Mating mediates morphophysiological changes in the spermathecae of Coptotermes gestroi queens |
title |
Mating mediates morphophysiological changes in the spermathecae of Coptotermes gestroi queens |
spellingShingle |
Mating mediates morphophysiological changes in the spermathecae of Coptotermes gestroi queens da Silva, Iago Bueno [UNESP] Blattaria Coptotermes gestroi eusocial insect histochemistry Isoptera pest species reproductive system Rhinotermitidae sperm storage spermatheca termite ultrastructure |
title_short |
Mating mediates morphophysiological changes in the spermathecae of Coptotermes gestroi queens |
title_full |
Mating mediates morphophysiological changes in the spermathecae of Coptotermes gestroi queens |
title_fullStr |
Mating mediates morphophysiological changes in the spermathecae of Coptotermes gestroi queens |
title_full_unstemmed |
Mating mediates morphophysiological changes in the spermathecae of Coptotermes gestroi queens |
title_sort |
Mating mediates morphophysiological changes in the spermathecae of Coptotermes gestroi queens |
author |
da Silva, Iago Bueno [UNESP] |
author_facet |
da Silva, Iago Bueno [UNESP] Costa-Leonardo, Ana Maria [UNESP] |
author_role |
author |
author2 |
Costa-Leonardo, Ana Maria [UNESP] |
author2_role |
author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
da Silva, Iago Bueno [UNESP] Costa-Leonardo, Ana Maria [UNESP] |
dc.subject.por.fl_str_mv |
Blattaria Coptotermes gestroi eusocial insect histochemistry Isoptera pest species reproductive system Rhinotermitidae sperm storage spermatheca termite ultrastructure |
topic |
Blattaria Coptotermes gestroi eusocial insect histochemistry Isoptera pest species reproductive system Rhinotermitidae sperm storage spermatheca termite ultrastructure |
description |
Insect spermathecae play a crucial role in sperm storage and maintenance prior to egg fertilization. Within eusocial insects, this structure is well studied in the Hymenoptera, whose queens copulate during a short period early in life and store sperm for up to decades. Within Isoptera, sperm storage and maintenance inside the spermatheca are poorly understood, especially due to the presence of a sperm-providing king. Here, we compared the morphometric and morphophysiological features of the spermathecae of virgin and mated queens of the invasive termite Coptotermes gestroi (Wasmann) (Isoptera: Rhinotermitidae). The spermatheca comprises a finger-shaped reservoir divided into two regions and a duct limited by a narrow transition. The superficial spermatheca area, as well as the luminal area, increase significantly after insemination, even among queens whose reproductive activity was reduced, suggesting that sperm storage continues during such conditions. The secretion of proteins and polysaccharides into the spermathecal lumen was a remarkable feature for both virgin and 4-year-old queens, although the concentration of the secreted content increased in the latter group. It suggests that spermatheca activation occurs before pairing, but its secretory activity intensifies to nourish and provide energy for the stored spermatozoa. Ultrastructure of the spermathecal epithelium showed a bicellular unit, composed of a secretory cell and associated canal cells. Secretory vesicles of various electron densities were observed next to the receiving canal of the secretory cells in both virgin and 2-year-old queens. Nevertheless, strongly electron-dense vesicles were only recorded for mated queens, which were associated with the increasing synthesis of proteins. The occurrence of rough endoplasmic reticulum and richness of mitochondria reinforces the protein synthesis and transport of contents towards the spermathecal lumen. In conclusion, the spermatheca of C. gestroi undergoes morphometric and physiological changes after mating, and further analysis may provide insights into the chemical nature of the spermathecal secretion prior to and after this event. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-07-29T13:27:37Z 2023-07-29T13:27:37Z 2023-05-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1111/eea.13253 Entomologia Experimentalis et Applicata, v. 171, n. 5, p. 361-373, 2023. 1570-7458 0013-8703 http://hdl.handle.net/11449/247850 10.1111/eea.13253 2-s2.0-85141538510 |
url |
http://dx.doi.org/10.1111/eea.13253 http://hdl.handle.net/11449/247850 |
identifier_str_mv |
Entomologia Experimentalis et Applicata, v. 171, n. 5, p. 361-373, 2023. 1570-7458 0013-8703 10.1111/eea.13253 2-s2.0-85141538510 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Entomologia Experimentalis et Applicata |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
361-373 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128468242661376 |