Angiotensin II, progesterone, and prostaglandins are sequential steps in the pathway to bovine oocyte nuclear maturation
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.theriogenology.2011.12.022 http://hdl.handle.net/11449/17842 |
Resumo: | Oocyte meiotic resumption is triggered by the ovulatory gonadotropin surge; in cattle, angiotensin II (AngII) and prostaglandins (PG) are key mediators of this gonadotropin-induced event. Here, we tested the hypothesis that progesterone (P-4) is also involved in oocyte meiotic resumption induced by the gonadotropin surge. In Experiment I, P-4 induced nuclear maturation in a dose-dependent manner using a coculture of follicular hemisections and cumulus-oocyte complexes. In the second experiment, using an in vivo model, an injection of mifepristone (MIFE; P-4 receptor antagonist) at the antrum of preovulatory follicles prevented GnRH-induced oocyte meiotic resumption in vivo. In Experiment III (coculture system similar to that of Experiment I), MIFE prevented stimulatory effects of AngII on resumption of meiosis, but saralasin (AngII receptor antagonist) did not inhibit P-4 actions. In Experiments IV and V, fibroblast growth Factor 10 (FGF10; known to suppress steroidogenesis in granulosa cells), blocked AngII-but not P-4-induced oocyte meiotic resumption. Therefore, we inferred that AngII is upstream to P-4 in a cascade to induce meiotic resumption. Previously, we had reported that AngII acted throughout the PGs pathway to modulate nuclear progression. In Experiment V, indomethacin inhibited resumption of meiosis induced by P-4, providing further support to the AngII-P-4, sequential effect on meiotic resumption. In conclusion, we inferred that AngII, P-4, and PGs are sequential steps in the same pathway that culminates with bovine oocyte maturation. (C) 2012 Elsevier B.V. All rights reserved. |
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Repositório Institucional da UNESP |
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Angiotensin II, progesterone, and prostaglandins are sequential steps in the pathway to bovine oocyte nuclear maturationOvulationMeiotic resumptionAngiotensinSteroidEicosanoidOocyte meiotic resumption is triggered by the ovulatory gonadotropin surge; in cattle, angiotensin II (AngII) and prostaglandins (PG) are key mediators of this gonadotropin-induced event. Here, we tested the hypothesis that progesterone (P-4) is also involved in oocyte meiotic resumption induced by the gonadotropin surge. In Experiment I, P-4 induced nuclear maturation in a dose-dependent manner using a coculture of follicular hemisections and cumulus-oocyte complexes. In the second experiment, using an in vivo model, an injection of mifepristone (MIFE; P-4 receptor antagonist) at the antrum of preovulatory follicles prevented GnRH-induced oocyte meiotic resumption in vivo. In Experiment III (coculture system similar to that of Experiment I), MIFE prevented stimulatory effects of AngII on resumption of meiosis, but saralasin (AngII receptor antagonist) did not inhibit P-4 actions. In Experiments IV and V, fibroblast growth Factor 10 (FGF10; known to suppress steroidogenesis in granulosa cells), blocked AngII-but not P-4-induced oocyte meiotic resumption. Therefore, we inferred that AngII is upstream to P-4 in a cascade to induce meiotic resumption. Previously, we had reported that AngII acted throughout the PGs pathway to modulate nuclear progression. In Experiment V, indomethacin inhibited resumption of meiosis induced by P-4, providing further support to the AngII-P-4, sequential effect on meiotic resumption. In conclusion, we inferred that AngII, P-4, and PGs are sequential steps in the same pathway that culminates with bovine oocyte maturation. (C) 2012 Elsevier B.V. All rights reserved.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Universidade Federal de Santa Maria (UFSM), Lab Biotechnol & Anim Reprod BioRep, BR-97119900 Santa Maria, RS, BrazilState Univ São Paulo UNESP, Dept Physiol, Botucatu, SP, BrazilState Univ São Paulo UNESP, Dept Physiol, Botucatu, SP, BrazilElsevier B.V.Universidade Federal de Santa Maria (UFSM)Universidade Estadual Paulista (Unesp)Siqueira, Lucas CarvalhoBarreta, Marcos HenriqueGasperin, BernardoBohrer, RodrigoSantos, Joabel TonellottoBuratini Junior, Jose [UNESP]Oliveira, Joao FranciscoGoncalves, Paulo Bayard2014-05-20T13:50:02Z2014-05-20T13:50:02Z2012-06-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1779-1787application/pdfhttp://dx.doi.org/10.1016/j.theriogenology.2011.12.022Theriogenology. New York: Elsevier B.V., v. 77, n. 9, p. 1779-1787, 2012.0093-691Xhttp://hdl.handle.net/11449/1784210.1016/j.theriogenology.2011.12.022WOS:000304231600006WOS000304231600006.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengTheriogenology2.136info:eu-repo/semantics/openAccess2023-10-18T06:10:10Zoai:repositorio.unesp.br:11449/17842Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:15:35.670895Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Angiotensin II, progesterone, and prostaglandins are sequential steps in the pathway to bovine oocyte nuclear maturation |
title |
Angiotensin II, progesterone, and prostaglandins are sequential steps in the pathway to bovine oocyte nuclear maturation |
spellingShingle |
Angiotensin II, progesterone, and prostaglandins are sequential steps in the pathway to bovine oocyte nuclear maturation Siqueira, Lucas Carvalho Ovulation Meiotic resumption Angiotensin Steroid Eicosanoid |
title_short |
Angiotensin II, progesterone, and prostaglandins are sequential steps in the pathway to bovine oocyte nuclear maturation |
title_full |
Angiotensin II, progesterone, and prostaglandins are sequential steps in the pathway to bovine oocyte nuclear maturation |
title_fullStr |
Angiotensin II, progesterone, and prostaglandins are sequential steps in the pathway to bovine oocyte nuclear maturation |
title_full_unstemmed |
Angiotensin II, progesterone, and prostaglandins are sequential steps in the pathway to bovine oocyte nuclear maturation |
title_sort |
Angiotensin II, progesterone, and prostaglandins are sequential steps in the pathway to bovine oocyte nuclear maturation |
author |
Siqueira, Lucas Carvalho |
author_facet |
Siqueira, Lucas Carvalho Barreta, Marcos Henrique Gasperin, Bernardo Bohrer, Rodrigo Santos, Joabel Tonellotto Buratini Junior, Jose [UNESP] Oliveira, Joao Francisco Goncalves, Paulo Bayard |
author_role |
author |
author2 |
Barreta, Marcos Henrique Gasperin, Bernardo Bohrer, Rodrigo Santos, Joabel Tonellotto Buratini Junior, Jose [UNESP] Oliveira, Joao Francisco Goncalves, Paulo Bayard |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Federal de Santa Maria (UFSM) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Siqueira, Lucas Carvalho Barreta, Marcos Henrique Gasperin, Bernardo Bohrer, Rodrigo Santos, Joabel Tonellotto Buratini Junior, Jose [UNESP] Oliveira, Joao Francisco Goncalves, Paulo Bayard |
dc.subject.por.fl_str_mv |
Ovulation Meiotic resumption Angiotensin Steroid Eicosanoid |
topic |
Ovulation Meiotic resumption Angiotensin Steroid Eicosanoid |
description |
Oocyte meiotic resumption is triggered by the ovulatory gonadotropin surge; in cattle, angiotensin II (AngII) and prostaglandins (PG) are key mediators of this gonadotropin-induced event. Here, we tested the hypothesis that progesterone (P-4) is also involved in oocyte meiotic resumption induced by the gonadotropin surge. In Experiment I, P-4 induced nuclear maturation in a dose-dependent manner using a coculture of follicular hemisections and cumulus-oocyte complexes. In the second experiment, using an in vivo model, an injection of mifepristone (MIFE; P-4 receptor antagonist) at the antrum of preovulatory follicles prevented GnRH-induced oocyte meiotic resumption in vivo. In Experiment III (coculture system similar to that of Experiment I), MIFE prevented stimulatory effects of AngII on resumption of meiosis, but saralasin (AngII receptor antagonist) did not inhibit P-4 actions. In Experiments IV and V, fibroblast growth Factor 10 (FGF10; known to suppress steroidogenesis in granulosa cells), blocked AngII-but not P-4-induced oocyte meiotic resumption. Therefore, we inferred that AngII is upstream to P-4 in a cascade to induce meiotic resumption. Previously, we had reported that AngII acted throughout the PGs pathway to modulate nuclear progression. In Experiment V, indomethacin inhibited resumption of meiosis induced by P-4, providing further support to the AngII-P-4, sequential effect on meiotic resumption. In conclusion, we inferred that AngII, P-4, and PGs are sequential steps in the same pathway that culminates with bovine oocyte maturation. (C) 2012 Elsevier B.V. All rights reserved. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-06-01 2014-05-20T13:50:02Z 2014-05-20T13:50:02Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.theriogenology.2011.12.022 Theriogenology. New York: Elsevier B.V., v. 77, n. 9, p. 1779-1787, 2012. 0093-691X http://hdl.handle.net/11449/17842 10.1016/j.theriogenology.2011.12.022 WOS:000304231600006 WOS000304231600006.pdf |
url |
http://dx.doi.org/10.1016/j.theriogenology.2011.12.022 http://hdl.handle.net/11449/17842 |
identifier_str_mv |
Theriogenology. New York: Elsevier B.V., v. 77, n. 9, p. 1779-1787, 2012. 0093-691X 10.1016/j.theriogenology.2011.12.022 WOS:000304231600006 WOS000304231600006.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Theriogenology 2.136 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1779-1787 application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128490630807552 |