Extraction and Characterization of Hemicellulose from Eucalyptus By-product: Assessment of Enzymatic Hydrolysis to Produce Xylooligosaccharides
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s12010-019-03076-0 http://hdl.handle.net/11449/189428 |
Resumo: | Eucalyptus wood is the primary source of fibers to produce paper and cellulose in South American countries. The major by-product generated in the cellulose industry is sawdust derived from chip wood production, which is designated as Eucalyptus by-product (EB). The xylooligosaccharides (XOS) are xylose-based oligomers with proven effects over maintenance and stimulation of beneficial human gut bacteria. This study reported the EB extraction and characterization along with an assessment of hemicellulose hydrolysis using commercial xylanases to produce XOS. Hemicellulose derived from extracted and NaClO2 pretreated (HEEBPT) presented xylan content of 55%, which was similar to 58.5% found in commercial Birchwood hemicellulose (CBH). The enzymatic hydrolysis of HEEBPT and CBH presented 30% as maximum conversion of xylan into XOS without significant difference among the enzymatic extracts evaluated. The XOS production from EB was proven as a technically feasible alternative to recover a value-added product from hemicellulosic fraction generated in the cellulose industry. However, lignin removal with NaClO2 from EB affects the feasibility of an industrial process because they generate toxic compounds in the pretreatment step. Thus, further studies with alternative reagents, such as ionic liquids, are required to asses selectively lignin removal from EB. [Figure not available: see fulltext.]. |
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Extraction and Characterization of Hemicellulose from Eucalyptus By-product: Assessment of Enzymatic Hydrolysis to Produce XylooligosaccharidesChemical compositionEnzymatic hydrolysisEucalyptus by-productHemicellulose extractionXylooligosaccharidesEucalyptus wood is the primary source of fibers to produce paper and cellulose in South American countries. The major by-product generated in the cellulose industry is sawdust derived from chip wood production, which is designated as Eucalyptus by-product (EB). The xylooligosaccharides (XOS) are xylose-based oligomers with proven effects over maintenance and stimulation of beneficial human gut bacteria. This study reported the EB extraction and characterization along with an assessment of hemicellulose hydrolysis using commercial xylanases to produce XOS. Hemicellulose derived from extracted and NaClO2 pretreated (HEEBPT) presented xylan content of 55%, which was similar to 58.5% found in commercial Birchwood hemicellulose (CBH). The enzymatic hydrolysis of HEEBPT and CBH presented 30% as maximum conversion of xylan into XOS without significant difference among the enzymatic extracts evaluated. The XOS production from EB was proven as a technically feasible alternative to recover a value-added product from hemicellulosic fraction generated in the cellulose industry. However, lignin removal with NaClO2 from EB affects the feasibility of an industrial process because they generate toxic compounds in the pretreatment step. Thus, further studies with alternative reagents, such as ionic liquids, are required to asses selectively lignin removal from EB. [Figure not available: see fulltext.].School of Pharmaceutical Sciences (FCF) Department of Bioprocesses and Biotechnology São Paulo State University (UNESP)School of Pharmaceutical Sciences (FCF) Department of Food and Nutrition São Paulo State University (UNESP)School of Pharmaceutical Sciences (FCF) Department of Bioprocesses and Biotechnology São Paulo State University (UNESP)School of Pharmaceutical Sciences (FCF) Department of Food and Nutrition São Paulo State University (UNESP)Universidade Estadual Paulista (Unesp)Mafei, Thamyres Del Torto [UNESP]Neto, Flávia Sanchez Penalva Pinto [UNESP]Peixoto, Guilherme [UNESP]de Baptista Neto, Álvaro [UNESP]Monti, Rubens [UNESP]Masarin, Fernando [UNESP]2019-10-06T16:40:24Z2019-10-06T16:40:24Z2019-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1007/s12010-019-03076-0Applied Biochemistry and Biotechnology.1559-02910273-2289http://hdl.handle.net/11449/18942810.1007/s12010-019-03076-02-s2.0-85069448180Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengApplied Biochemistry and Biotechnologyinfo:eu-repo/semantics/openAccess2021-10-23T01:35:31Zoai:repositorio.unesp.br:11449/189428Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T01:35:31Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Extraction and Characterization of Hemicellulose from Eucalyptus By-product: Assessment of Enzymatic Hydrolysis to Produce Xylooligosaccharides |
title |
Extraction and Characterization of Hemicellulose from Eucalyptus By-product: Assessment of Enzymatic Hydrolysis to Produce Xylooligosaccharides |
spellingShingle |
Extraction and Characterization of Hemicellulose from Eucalyptus By-product: Assessment of Enzymatic Hydrolysis to Produce Xylooligosaccharides Mafei, Thamyres Del Torto [UNESP] Chemical composition Enzymatic hydrolysis Eucalyptus by-product Hemicellulose extraction Xylooligosaccharides |
title_short |
Extraction and Characterization of Hemicellulose from Eucalyptus By-product: Assessment of Enzymatic Hydrolysis to Produce Xylooligosaccharides |
title_full |
Extraction and Characterization of Hemicellulose from Eucalyptus By-product: Assessment of Enzymatic Hydrolysis to Produce Xylooligosaccharides |
title_fullStr |
Extraction and Characterization of Hemicellulose from Eucalyptus By-product: Assessment of Enzymatic Hydrolysis to Produce Xylooligosaccharides |
title_full_unstemmed |
Extraction and Characterization of Hemicellulose from Eucalyptus By-product: Assessment of Enzymatic Hydrolysis to Produce Xylooligosaccharides |
title_sort |
Extraction and Characterization of Hemicellulose from Eucalyptus By-product: Assessment of Enzymatic Hydrolysis to Produce Xylooligosaccharides |
author |
Mafei, Thamyres Del Torto [UNESP] |
author_facet |
Mafei, Thamyres Del Torto [UNESP] Neto, Flávia Sanchez Penalva Pinto [UNESP] Peixoto, Guilherme [UNESP] de Baptista Neto, Álvaro [UNESP] Monti, Rubens [UNESP] Masarin, Fernando [UNESP] |
author_role |
author |
author2 |
Neto, Flávia Sanchez Penalva Pinto [UNESP] Peixoto, Guilherme [UNESP] de Baptista Neto, Álvaro [UNESP] Monti, Rubens [UNESP] Masarin, Fernando [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Mafei, Thamyres Del Torto [UNESP] Neto, Flávia Sanchez Penalva Pinto [UNESP] Peixoto, Guilherme [UNESP] de Baptista Neto, Álvaro [UNESP] Monti, Rubens [UNESP] Masarin, Fernando [UNESP] |
dc.subject.por.fl_str_mv |
Chemical composition Enzymatic hydrolysis Eucalyptus by-product Hemicellulose extraction Xylooligosaccharides |
topic |
Chemical composition Enzymatic hydrolysis Eucalyptus by-product Hemicellulose extraction Xylooligosaccharides |
description |
Eucalyptus wood is the primary source of fibers to produce paper and cellulose in South American countries. The major by-product generated in the cellulose industry is sawdust derived from chip wood production, which is designated as Eucalyptus by-product (EB). The xylooligosaccharides (XOS) are xylose-based oligomers with proven effects over maintenance and stimulation of beneficial human gut bacteria. This study reported the EB extraction and characterization along with an assessment of hemicellulose hydrolysis using commercial xylanases to produce XOS. Hemicellulose derived from extracted and NaClO2 pretreated (HEEBPT) presented xylan content of 55%, which was similar to 58.5% found in commercial Birchwood hemicellulose (CBH). The enzymatic hydrolysis of HEEBPT and CBH presented 30% as maximum conversion of xylan into XOS without significant difference among the enzymatic extracts evaluated. The XOS production from EB was proven as a technically feasible alternative to recover a value-added product from hemicellulosic fraction generated in the cellulose industry. However, lignin removal with NaClO2 from EB affects the feasibility of an industrial process because they generate toxic compounds in the pretreatment step. Thus, further studies with alternative reagents, such as ionic liquids, are required to asses selectively lignin removal from EB. [Figure not available: see fulltext.]. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-06T16:40:24Z 2019-10-06T16:40:24Z 2019-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s12010-019-03076-0 Applied Biochemistry and Biotechnology. 1559-0291 0273-2289 http://hdl.handle.net/11449/189428 10.1007/s12010-019-03076-0 2-s2.0-85069448180 |
url |
http://dx.doi.org/10.1007/s12010-019-03076-0 http://hdl.handle.net/11449/189428 |
identifier_str_mv |
Applied Biochemistry and Biotechnology. 1559-0291 0273-2289 10.1007/s12010-019-03076-0 2-s2.0-85069448180 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Applied Biochemistry and Biotechnology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965249331265536 |