Performance of microbiological, serological, molecular, and modified seminal plasma methods in the diagnosis of Brucella abortus in semen and serum of bovine bulls
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.biologicals.2017.06.005 http://hdl.handle.net/11449/174830 |
Resumo: | Brucellosis remains as a major infectious disease of domestic animals and is considered a re-emerging zoonosis in several countries. B. abortus infections in bulls are related to reproductive tract infections, although infected animals show transient serological titers or nonreactor status. Thus, diagnosis of bovine brucellosis based exclusively on serological tests probably underestimates B. abortus infections in bulls. In this scenario, three hundred thirty-five serum samples from reproductively mature bovine bulls were subjected simultaneously to standard serodiagnosis using the rose Bengal test (RBT), 2-mercaptoethanol (2-ME), complement fixation (CFT), and fluorescence polarization assay (FPA). Furthermore, conventional semen plasma agglutination (SPA) and modified 2-ME, FC and, FPA were carried out in all bulls replaing serum by seminal plasma. Semen from all bulls was also analyzed for sperm viability, microbiological culture in Farrell media, and polymerase chain reaction (PCR). Only eight (2.38%) semen samples were considered improper for reproduction services (necrospermia and azoospermia), although none of these animals was positive in any of the diagnosis methods used. Five bulls (1.49%) were simultaneously positive in conventional RBT, 2-ME, SPA, modified 2-ME, microbiological culture in Farrell media, and in PCR for B. abortus strain 19. Two (1.67%) bulls were positive in PCR for B. abortus field strains and negative in all other tests, although semen was considered viable to reproduction service. The identification of B. abortus B19 strain in serum and semen of bulls occurred probably due to improper vaccination of males or infection by B19 strain shedding by vaccinated females that could to contaminated environment of farms. In addition, detection of B. abortus field strains only using PCR in bulls without sperm viability abnormalities indicate the need for including molecular methods to improve diagnosis of the disease in bovine bulls. |
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Performance of microbiological, serological, molecular, and modified seminal plasma methods in the diagnosis of Brucella abortus in semen and serum of bovine bullsBrucellosisMalesPCRSeminal plasmaSerodiagnosisBrucellosis remains as a major infectious disease of domestic animals and is considered a re-emerging zoonosis in several countries. B. abortus infections in bulls are related to reproductive tract infections, although infected animals show transient serological titers or nonreactor status. Thus, diagnosis of bovine brucellosis based exclusively on serological tests probably underestimates B. abortus infections in bulls. In this scenario, three hundred thirty-five serum samples from reproductively mature bovine bulls were subjected simultaneously to standard serodiagnosis using the rose Bengal test (RBT), 2-mercaptoethanol (2-ME), complement fixation (CFT), and fluorescence polarization assay (FPA). Furthermore, conventional semen plasma agglutination (SPA) and modified 2-ME, FC and, FPA were carried out in all bulls replaing serum by seminal plasma. Semen from all bulls was also analyzed for sperm viability, microbiological culture in Farrell media, and polymerase chain reaction (PCR). Only eight (2.38%) semen samples were considered improper for reproduction services (necrospermia and azoospermia), although none of these animals was positive in any of the diagnosis methods used. Five bulls (1.49%) were simultaneously positive in conventional RBT, 2-ME, SPA, modified 2-ME, microbiological culture in Farrell media, and in PCR for B. abortus strain 19. Two (1.67%) bulls were positive in PCR for B. abortus field strains and negative in all other tests, although semen was considered viable to reproduction service. The identification of B. abortus B19 strain in serum and semen of bulls occurred probably due to improper vaccination of males or infection by B19 strain shedding by vaccinated females that could to contaminated environment of farms. In addition, detection of B. abortus field strains only using PCR in bulls without sperm viability abnormalities indicate the need for including molecular methods to improve diagnosis of the disease in bovine bulls.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Tecnologia em Agronegócio Faculdade de Tecnologia de Botucatu (FATEC)Departamento de Higiene Veterinária e Saúde Pública Faculdade de Medicina Veterinária e Zootecnia Universidade Estadual Paulista (UNESP)Departamento de Medicina Veterinária Preventiva e Reprodução Animal Faculdade de Ciências Agrárias e Veterinárias UNESPLaboratório de Doenças Bacterianas da Reprodução do Centro de Pesquisa e Desenvolvimento de Sanidade Animal Instituto Biológico de São PauloDoenças Infecciosas dos Animais Universidade de Santo AmaroReprodução Animal Universidade do Oeste Paulista (UNOESTE)Agência Paulista de Tecnologia em Agronegócio (APTA) Bovinos de Corte Instituto de ZootecniaDepartamento de Higiene Veterinária e Saúde Pública Faculdade de Medicina Veterinária e Zootecnia Universidade Estadual Paulista (UNESP)Departamento de Medicina Veterinária Preventiva e Reprodução Animal Faculdade de Ciências Agrárias e Veterinárias UNESPFAPESP: 2011/20263-0Faculdade de Tecnologia de Botucatu (FATEC)Universidade Estadual Paulista (Unesp)Instituto Biológico de São PauloUniversidade de Santo AmaroUniversidade do Oeste Paulista (UNOESTE)Instituto de ZootecniaNardi Júnior, G.Megid, J. [UNESP]Mathias, L. A. [UNESP]Paulin, L.Vicente, A. F. [UNESP]Cortez, A.Listoni, F. J.P. [UNESP]Lara, G. H.B. [UNESP]Motta, R. G. [UNESP]Chacur, M. G.M.Monteiro, F. M.Ribeiro, M. G. [UNESP]2018-12-11T17:13:04Z2018-12-11T17:13:04Z2017-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article6-9application/pdfhttp://dx.doi.org/10.1016/j.biologicals.2017.06.005Biologicals, v. 48, p. 6-9.1095-83201045-1056http://hdl.handle.net/11449/17483010.1016/j.biologicals.2017.06.0052-s2.0-850217024542-s2.0-85021702454.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBiologicals0,6360,636info:eu-repo/semantics/openAccess2024-06-06T18:10:26Zoai:repositorio.unesp.br:11449/174830Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:39:03.764171Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Performance of microbiological, serological, molecular, and modified seminal plasma methods in the diagnosis of Brucella abortus in semen and serum of bovine bulls |
title |
Performance of microbiological, serological, molecular, and modified seminal plasma methods in the diagnosis of Brucella abortus in semen and serum of bovine bulls |
spellingShingle |
Performance of microbiological, serological, molecular, and modified seminal plasma methods in the diagnosis of Brucella abortus in semen and serum of bovine bulls Nardi Júnior, G. Brucellosis Males PCR Seminal plasma Serodiagnosis |
title_short |
Performance of microbiological, serological, molecular, and modified seminal plasma methods in the diagnosis of Brucella abortus in semen and serum of bovine bulls |
title_full |
Performance of microbiological, serological, molecular, and modified seminal plasma methods in the diagnosis of Brucella abortus in semen and serum of bovine bulls |
title_fullStr |
Performance of microbiological, serological, molecular, and modified seminal plasma methods in the diagnosis of Brucella abortus in semen and serum of bovine bulls |
title_full_unstemmed |
Performance of microbiological, serological, molecular, and modified seminal plasma methods in the diagnosis of Brucella abortus in semen and serum of bovine bulls |
title_sort |
Performance of microbiological, serological, molecular, and modified seminal plasma methods in the diagnosis of Brucella abortus in semen and serum of bovine bulls |
author |
Nardi Júnior, G. |
author_facet |
Nardi Júnior, G. Megid, J. [UNESP] Mathias, L. A. [UNESP] Paulin, L. Vicente, A. F. [UNESP] Cortez, A. Listoni, F. J.P. [UNESP] Lara, G. H.B. [UNESP] Motta, R. G. [UNESP] Chacur, M. G.M. Monteiro, F. M. Ribeiro, M. G. [UNESP] |
author_role |
author |
author2 |
Megid, J. [UNESP] Mathias, L. A. [UNESP] Paulin, L. Vicente, A. F. [UNESP] Cortez, A. Listoni, F. J.P. [UNESP] Lara, G. H.B. [UNESP] Motta, R. G. [UNESP] Chacur, M. G.M. Monteiro, F. M. Ribeiro, M. G. [UNESP] |
author2_role |
author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Faculdade de Tecnologia de Botucatu (FATEC) Universidade Estadual Paulista (Unesp) Instituto Biológico de São Paulo Universidade de Santo Amaro Universidade do Oeste Paulista (UNOESTE) Instituto de Zootecnia |
dc.contributor.author.fl_str_mv |
Nardi Júnior, G. Megid, J. [UNESP] Mathias, L. A. [UNESP] Paulin, L. Vicente, A. F. [UNESP] Cortez, A. Listoni, F. J.P. [UNESP] Lara, G. H.B. [UNESP] Motta, R. G. [UNESP] Chacur, M. G.M. Monteiro, F. M. Ribeiro, M. G. [UNESP] |
dc.subject.por.fl_str_mv |
Brucellosis Males PCR Seminal plasma Serodiagnosis |
topic |
Brucellosis Males PCR Seminal plasma Serodiagnosis |
description |
Brucellosis remains as a major infectious disease of domestic animals and is considered a re-emerging zoonosis in several countries. B. abortus infections in bulls are related to reproductive tract infections, although infected animals show transient serological titers or nonreactor status. Thus, diagnosis of bovine brucellosis based exclusively on serological tests probably underestimates B. abortus infections in bulls. In this scenario, three hundred thirty-five serum samples from reproductively mature bovine bulls were subjected simultaneously to standard serodiagnosis using the rose Bengal test (RBT), 2-mercaptoethanol (2-ME), complement fixation (CFT), and fluorescence polarization assay (FPA). Furthermore, conventional semen plasma agglutination (SPA) and modified 2-ME, FC and, FPA were carried out in all bulls replaing serum by seminal plasma. Semen from all bulls was also analyzed for sperm viability, microbiological culture in Farrell media, and polymerase chain reaction (PCR). Only eight (2.38%) semen samples were considered improper for reproduction services (necrospermia and azoospermia), although none of these animals was positive in any of the diagnosis methods used. Five bulls (1.49%) were simultaneously positive in conventional RBT, 2-ME, SPA, modified 2-ME, microbiological culture in Farrell media, and in PCR for B. abortus strain 19. Two (1.67%) bulls were positive in PCR for B. abortus field strains and negative in all other tests, although semen was considered viable to reproduction service. The identification of B. abortus B19 strain in serum and semen of bulls occurred probably due to improper vaccination of males or infection by B19 strain shedding by vaccinated females that could to contaminated environment of farms. In addition, detection of B. abortus field strains only using PCR in bulls without sperm viability abnormalities indicate the need for including molecular methods to improve diagnosis of the disease in bovine bulls. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-07-01 2018-12-11T17:13:04Z 2018-12-11T17:13:04Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.biologicals.2017.06.005 Biologicals, v. 48, p. 6-9. 1095-8320 1045-1056 http://hdl.handle.net/11449/174830 10.1016/j.biologicals.2017.06.005 2-s2.0-85021702454 2-s2.0-85021702454.pdf |
url |
http://dx.doi.org/10.1016/j.biologicals.2017.06.005 http://hdl.handle.net/11449/174830 |
identifier_str_mv |
Biologicals, v. 48, p. 6-9. 1095-8320 1045-1056 10.1016/j.biologicals.2017.06.005 2-s2.0-85021702454 2-s2.0-85021702454.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Biologicals 0,636 0,636 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
6-9 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808129540179886080 |