Intestinal anti-inflammatory activity of Ground Cherry (Physalis angulata L.) standardized CO2 phytopharmaceutical preparation
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3748/wjg.v23.i24.4369 http://hdl.handle.net/11449/169866 |
Resumo: | AIM To investigate the effects of Ground Cherry (Physalis angulata L.) standardized supercritical CO2 extract in trinitrobenzenesulphonic acid (TNBS) model of rat intestinal inflammation. METHODS The animals were divided into groups that received vehicle or P. angulata extract (PACO2) orally at the doses 25, 50 and 100 mg/kg daily by 5 d before TNBS damage. Protective effects of PACO2 were assessed by macroscopic analysis, biochemical determinations of the levels of myeloperoxidase (MPO), alkaline phosphatase (ALP), glutathione and cytokines (such as INF-γ, IL-1β, IL-6, IL-10 and TNF-α), gene expression evaluation (including Hsp70, heparanase, NF-κB, mitogenactivated protein kinases (Mapk) 1, 3, 6 and 9, and the mucins genes Muc 1, 2, 3 and 4) and histopathological studies using optical, and electronic (transmission and scanning) microscopy. RESULTS PACO2 extract promoted a significant reduction in MPO and ALP activities, reducing oxidative stress and neutrophil infiltration. These effects were accompanied by significant reduction of colonic levels of IFN-γ and IL-6 and down-regulation of heparanase, Hsp70, Mapk3, Mapk9, Muc1 and Muc2 genes expression when compared with TNBS-control animals. In addition, protective effects were also evidenced by reduced neutrophil infiltration, recovery of cell architecture and replacement of mucin by histopathological and ultrastructural analysis. CONCLUSION Physalis angulata supercritical CO2 extract is an intestinal anti-inflammatory product that modulates oxidative stress, immune response and expression of inflammatory mediators, with potentially utility for treating inflammatory bowel disease. |
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Intestinal anti-inflammatory activity of Ground Cherry (Physalis angulata L.) standardized CO2 phytopharmaceutical preparationInflammatory bowel diseaseIntestinal diseasePhysalis angulata L. trinitrobenzenesulphonic acidAIM To investigate the effects of Ground Cherry (Physalis angulata L.) standardized supercritical CO2 extract in trinitrobenzenesulphonic acid (TNBS) model of rat intestinal inflammation. METHODS The animals were divided into groups that received vehicle or P. angulata extract (PACO2) orally at the doses 25, 50 and 100 mg/kg daily by 5 d before TNBS damage. Protective effects of PACO2 were assessed by macroscopic analysis, biochemical determinations of the levels of myeloperoxidase (MPO), alkaline phosphatase (ALP), glutathione and cytokines (such as INF-γ, IL-1β, IL-6, IL-10 and TNF-α), gene expression evaluation (including Hsp70, heparanase, NF-κB, mitogenactivated protein kinases (Mapk) 1, 3, 6 and 9, and the mucins genes Muc 1, 2, 3 and 4) and histopathological studies using optical, and electronic (transmission and scanning) microscopy. RESULTS PACO2 extract promoted a significant reduction in MPO and ALP activities, reducing oxidative stress and neutrophil infiltration. These effects were accompanied by significant reduction of colonic levels of IFN-γ and IL-6 and down-regulation of heparanase, Hsp70, Mapk3, Mapk9, Muc1 and Muc2 genes expression when compared with TNBS-control animals. In addition, protective effects were also evidenced by reduced neutrophil infiltration, recovery of cell architecture and replacement of mucin by histopathological and ultrastructural analysis. CONCLUSION Physalis angulata supercritical CO2 extract is an intestinal anti-inflammatory product that modulates oxidative stress, immune response and expression of inflammatory mediators, with potentially utility for treating inflammatory bowel disease.Laboratory of Phytomedicines Pharmacology and Biotechnology (PhytoPharmaTech) Department of Pharmacology Institute of Biosciences Universidade Estadual Paulista UNESPLaboratory of Phytomedicines Pharmacology and Biotechnology (PhytoPharmaTech) Department of Pharmacology Institute of Biosciences Universidade Estadual Paulista UNESPUniversidade Estadual Paulista (Unesp)Almeida, Luiz Domingues [UNESP]Quaglio, Ana Elisa Valencise [UNESP]De Almeida Costa, Celso Acácio Rodrigues [UNESP]Di Stasi, Luiz Claudio [UNESP]2018-12-11T16:47:56Z2018-12-11T16:47:56Z2017-06-28info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article4369-4380application/pdfhttp://dx.doi.org/10.3748/wjg.v23.i24.4369World Journal of Gastroenterology, v. 23, n. 24, p. 4369-4380, 2017.2219-28401007-9327http://hdl.handle.net/11449/16986610.3748/wjg.v23.i24.43692-s2.0-850213159072-s2.0-85021315907.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengWorld Journal of Gastroenterology1,4091,409info:eu-repo/semantics/openAccess2023-11-24T06:12:29Zoai:repositorio.unesp.br:11449/169866Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:34:19.652621Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Intestinal anti-inflammatory activity of Ground Cherry (Physalis angulata L.) standardized CO2 phytopharmaceutical preparation |
title |
Intestinal anti-inflammatory activity of Ground Cherry (Physalis angulata L.) standardized CO2 phytopharmaceutical preparation |
spellingShingle |
Intestinal anti-inflammatory activity of Ground Cherry (Physalis angulata L.) standardized CO2 phytopharmaceutical preparation Almeida, Luiz Domingues [UNESP] Inflammatory bowel disease Intestinal disease Physalis angulata L. trinitrobenzenesulphonic acid |
title_short |
Intestinal anti-inflammatory activity of Ground Cherry (Physalis angulata L.) standardized CO2 phytopharmaceutical preparation |
title_full |
Intestinal anti-inflammatory activity of Ground Cherry (Physalis angulata L.) standardized CO2 phytopharmaceutical preparation |
title_fullStr |
Intestinal anti-inflammatory activity of Ground Cherry (Physalis angulata L.) standardized CO2 phytopharmaceutical preparation |
title_full_unstemmed |
Intestinal anti-inflammatory activity of Ground Cherry (Physalis angulata L.) standardized CO2 phytopharmaceutical preparation |
title_sort |
Intestinal anti-inflammatory activity of Ground Cherry (Physalis angulata L.) standardized CO2 phytopharmaceutical preparation |
author |
Almeida, Luiz Domingues [UNESP] |
author_facet |
Almeida, Luiz Domingues [UNESP] Quaglio, Ana Elisa Valencise [UNESP] De Almeida Costa, Celso Acácio Rodrigues [UNESP] Di Stasi, Luiz Claudio [UNESP] |
author_role |
author |
author2 |
Quaglio, Ana Elisa Valencise [UNESP] De Almeida Costa, Celso Acácio Rodrigues [UNESP] Di Stasi, Luiz Claudio [UNESP] |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Almeida, Luiz Domingues [UNESP] Quaglio, Ana Elisa Valencise [UNESP] De Almeida Costa, Celso Acácio Rodrigues [UNESP] Di Stasi, Luiz Claudio [UNESP] |
dc.subject.por.fl_str_mv |
Inflammatory bowel disease Intestinal disease Physalis angulata L. trinitrobenzenesulphonic acid |
topic |
Inflammatory bowel disease Intestinal disease Physalis angulata L. trinitrobenzenesulphonic acid |
description |
AIM To investigate the effects of Ground Cherry (Physalis angulata L.) standardized supercritical CO2 extract in trinitrobenzenesulphonic acid (TNBS) model of rat intestinal inflammation. METHODS The animals were divided into groups that received vehicle or P. angulata extract (PACO2) orally at the doses 25, 50 and 100 mg/kg daily by 5 d before TNBS damage. Protective effects of PACO2 were assessed by macroscopic analysis, biochemical determinations of the levels of myeloperoxidase (MPO), alkaline phosphatase (ALP), glutathione and cytokines (such as INF-γ, IL-1β, IL-6, IL-10 and TNF-α), gene expression evaluation (including Hsp70, heparanase, NF-κB, mitogenactivated protein kinases (Mapk) 1, 3, 6 and 9, and the mucins genes Muc 1, 2, 3 and 4) and histopathological studies using optical, and electronic (transmission and scanning) microscopy. RESULTS PACO2 extract promoted a significant reduction in MPO and ALP activities, reducing oxidative stress and neutrophil infiltration. These effects were accompanied by significant reduction of colonic levels of IFN-γ and IL-6 and down-regulation of heparanase, Hsp70, Mapk3, Mapk9, Muc1 and Muc2 genes expression when compared with TNBS-control animals. In addition, protective effects were also evidenced by reduced neutrophil infiltration, recovery of cell architecture and replacement of mucin by histopathological and ultrastructural analysis. CONCLUSION Physalis angulata supercritical CO2 extract is an intestinal anti-inflammatory product that modulates oxidative stress, immune response and expression of inflammatory mediators, with potentially utility for treating inflammatory bowel disease. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-06-28 2018-12-11T16:47:56Z 2018-12-11T16:47:56Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3748/wjg.v23.i24.4369 World Journal of Gastroenterology, v. 23, n. 24, p. 4369-4380, 2017. 2219-2840 1007-9327 http://hdl.handle.net/11449/169866 10.3748/wjg.v23.i24.4369 2-s2.0-85021315907 2-s2.0-85021315907.pdf |
url |
http://dx.doi.org/10.3748/wjg.v23.i24.4369 http://hdl.handle.net/11449/169866 |
identifier_str_mv |
World Journal of Gastroenterology, v. 23, n. 24, p. 4369-4380, 2017. 2219-2840 1007-9327 10.3748/wjg.v23.i24.4369 2-s2.0-85021315907 2-s2.0-85021315907.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
World Journal of Gastroenterology 1,409 1,409 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
4369-4380 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808128950486958080 |