Expression of AIF, PARP, and miRNAs MIR-145, MIR-210, and MIR-486 Associated with Apoptosis in the Corpus Cavernosum of Rats Subjected to Chronic Alcoholism Model
Autor(a) principal: | |
---|---|
Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://hdl.handle.net/11449/209617 |
Resumo: | Previous studies from our group described the consequences of using ethanol on penile erection. Nevertheless, the molecular mechanisms surrounding microRNAs, apoptosis process and their relationship with erectile dysfunction associated with alcohol consumption are still poorly understood. The objective of this analysis was to evaluate the mechanism of apoptosis by the expression of AIF and PARP, as well as their regulatory microRNAs: miR-145, miR-210 and miR-486, in the corpus cavemosum of rats submitted to a semivoltmtary alcoholism model. For this study 24 Wistar rats were divided into two groups: control (C) and treated with 20 % ethanol (A) for seven weeks. The corpus cavernosum samples were prepared for immunohistochemical analysis of AIF and PARP protein expression, and microRNAs miR-145, miR-210, miR-486 gene expression in cavernous tissue was performed by real time PCR. The immunohistochemical analysis showed little nuclear positive labeling for the protein PARP and AIF in the corpus cavernosum of control and ethanol treated animals. After analysis of miR-145, -210 and -486 microRNA expression in the 12 animals studied, no results were found with significant statistical difference between the control and alcoholized groups. The expression ofAIF and PARP and their regulatory inicroRNAs involved in apoptotic process (miR-145, miR-210 and miR-486) were not altered in the corpus cavernosum of rats submitted to semivoltmtary alcoholism. |
id |
UNSP_ca91c61c069afcdaddf999be4da44dd3 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/209617 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Expression of AIF, PARP, and miRNAs MIR-145, MIR-210, and MIR-486 Associated with Apoptosis in the Corpus Cavernosum of Rats Subjected to Chronic Alcoholism ModelAlcoholismApoptosisErectile DysfunctionMicroRNAsPrevious studies from our group described the consequences of using ethanol on penile erection. Nevertheless, the molecular mechanisms surrounding microRNAs, apoptosis process and their relationship with erectile dysfunction associated with alcohol consumption are still poorly understood. The objective of this analysis was to evaluate the mechanism of apoptosis by the expression of AIF and PARP, as well as their regulatory microRNAs: miR-145, miR-210 and miR-486, in the corpus cavemosum of rats submitted to a semivoltmtary alcoholism model. For this study 24 Wistar rats were divided into two groups: control (C) and treated with 20 % ethanol (A) for seven weeks. The corpus cavernosum samples were prepared for immunohistochemical analysis of AIF and PARP protein expression, and microRNAs miR-145, miR-210, miR-486 gene expression in cavernous tissue was performed by real time PCR. The immunohistochemical analysis showed little nuclear positive labeling for the protein PARP and AIF in the corpus cavernosum of control and ethanol treated animals. After analysis of miR-145, -210 and -486 microRNA expression in the 12 animals studied, no results were found with significant statistical difference between the control and alcoholized groups. The expression ofAIF and PARP and their regulatory inicroRNAs involved in apoptotic process (miR-145, miR-210 and miR-486) were not altered in the corpus cavernosum of rats submitted to semivoltmtary alcoholism.Sao Paulo Univ USP, Med Sch Ribeirao Preto, Dept Surg & Anat, Ribeirao Preto, SP, BrazilUniv Ribeirao Preto, Dept Med, Ribeirao Preto, BrazilUNESP, Biosci Inst Botucatu, Dept Anat, Botucatu, SP, BrazilUniv Fed Sao Carlos, Dept Morphol & Pathol, Sao Carlos, SP, BrazilUniv Marilia, Dept Odontol, Marilia, SP, BrazilUniv Marilia, Postgrad Program Struct & Funct Interact Rehabil, Marilia, SP, BrazilUNESP, Biosci Inst Botucatu, Dept Anat, Botucatu, SP, BrazilSoc Chilena AnatomiaUniversidade de São Paulo (USP)Univ Ribeirao PretoUniversidade Estadual Paulista (Unesp)Universidade Federal de São Carlos (UFSCar)Univ MariliaSarraipo, Vagner SchiavoniLizarte Neto, Fermino SanchesMello de Carvalho, Camila AlbuquerqueSilva, Jairo Pinheiro daDurand, Marina ToledoSchimming, Bruno Cezar [UNESP]Martinez, MarceloTazima Sorita Galli, Maria de FatimaBuono, Fabianna de OliveiraBarbirato da Mata Tiezzi, Maria FernandaNovais, Paulo CezarGula, Isabella StracieriThomazini, Jose AntonioSassoli Fazan, Valeria PaulaRodrigues, Andressa RomualdoCunha Tirapelli, Daniela Pretti daAssis Cirino, Mucio Luiz deFernandes Molina, Carlos AugustoTucci Junior, SilvioTirapelli, Luis Fernando2021-06-25T12:24:03Z2021-06-25T12:24:03Z2020-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1639-1644International Journal Of Morphology. Temuco: Soc Chilena Anatomia, v. 38, n. 6, p. 1639-1644, 2020.0717-9502http://hdl.handle.net/11449/209617WOS:000585776700021Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInternational Journal Of Morphologyinfo:eu-repo/semantics/openAccess2021-10-23T19:28:19Zoai:repositorio.unesp.br:11449/209617Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:40:45.432258Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Expression of AIF, PARP, and miRNAs MIR-145, MIR-210, and MIR-486 Associated with Apoptosis in the Corpus Cavernosum of Rats Subjected to Chronic Alcoholism Model |
title |
Expression of AIF, PARP, and miRNAs MIR-145, MIR-210, and MIR-486 Associated with Apoptosis in the Corpus Cavernosum of Rats Subjected to Chronic Alcoholism Model |
spellingShingle |
Expression of AIF, PARP, and miRNAs MIR-145, MIR-210, and MIR-486 Associated with Apoptosis in the Corpus Cavernosum of Rats Subjected to Chronic Alcoholism Model Sarraipo, Vagner Schiavoni Alcoholism Apoptosis Erectile Dysfunction MicroRNAs |
title_short |
Expression of AIF, PARP, and miRNAs MIR-145, MIR-210, and MIR-486 Associated with Apoptosis in the Corpus Cavernosum of Rats Subjected to Chronic Alcoholism Model |
title_full |
Expression of AIF, PARP, and miRNAs MIR-145, MIR-210, and MIR-486 Associated with Apoptosis in the Corpus Cavernosum of Rats Subjected to Chronic Alcoholism Model |
title_fullStr |
Expression of AIF, PARP, and miRNAs MIR-145, MIR-210, and MIR-486 Associated with Apoptosis in the Corpus Cavernosum of Rats Subjected to Chronic Alcoholism Model |
title_full_unstemmed |
Expression of AIF, PARP, and miRNAs MIR-145, MIR-210, and MIR-486 Associated with Apoptosis in the Corpus Cavernosum of Rats Subjected to Chronic Alcoholism Model |
title_sort |
Expression of AIF, PARP, and miRNAs MIR-145, MIR-210, and MIR-486 Associated with Apoptosis in the Corpus Cavernosum of Rats Subjected to Chronic Alcoholism Model |
author |
Sarraipo, Vagner Schiavoni |
author_facet |
Sarraipo, Vagner Schiavoni Lizarte Neto, Fermino Sanches Mello de Carvalho, Camila Albuquerque Silva, Jairo Pinheiro da Durand, Marina Toledo Schimming, Bruno Cezar [UNESP] Martinez, Marcelo Tazima Sorita Galli, Maria de Fatima Buono, Fabianna de Oliveira Barbirato da Mata Tiezzi, Maria Fernanda Novais, Paulo Cezar Gula, Isabella Stracieri Thomazini, Jose Antonio Sassoli Fazan, Valeria Paula Rodrigues, Andressa Romualdo Cunha Tirapelli, Daniela Pretti da Assis Cirino, Mucio Luiz de Fernandes Molina, Carlos Augusto Tucci Junior, Silvio Tirapelli, Luis Fernando |
author_role |
author |
author2 |
Lizarte Neto, Fermino Sanches Mello de Carvalho, Camila Albuquerque Silva, Jairo Pinheiro da Durand, Marina Toledo Schimming, Bruno Cezar [UNESP] Martinez, Marcelo Tazima Sorita Galli, Maria de Fatima Buono, Fabianna de Oliveira Barbirato da Mata Tiezzi, Maria Fernanda Novais, Paulo Cezar Gula, Isabella Stracieri Thomazini, Jose Antonio Sassoli Fazan, Valeria Paula Rodrigues, Andressa Romualdo Cunha Tirapelli, Daniela Pretti da Assis Cirino, Mucio Luiz de Fernandes Molina, Carlos Augusto Tucci Junior, Silvio Tirapelli, Luis Fernando |
author2_role |
author author author author author author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Univ Ribeirao Preto Universidade Estadual Paulista (Unesp) Universidade Federal de São Carlos (UFSCar) Univ Marilia |
dc.contributor.author.fl_str_mv |
Sarraipo, Vagner Schiavoni Lizarte Neto, Fermino Sanches Mello de Carvalho, Camila Albuquerque Silva, Jairo Pinheiro da Durand, Marina Toledo Schimming, Bruno Cezar [UNESP] Martinez, Marcelo Tazima Sorita Galli, Maria de Fatima Buono, Fabianna de Oliveira Barbirato da Mata Tiezzi, Maria Fernanda Novais, Paulo Cezar Gula, Isabella Stracieri Thomazini, Jose Antonio Sassoli Fazan, Valeria Paula Rodrigues, Andressa Romualdo Cunha Tirapelli, Daniela Pretti da Assis Cirino, Mucio Luiz de Fernandes Molina, Carlos Augusto Tucci Junior, Silvio Tirapelli, Luis Fernando |
dc.subject.por.fl_str_mv |
Alcoholism Apoptosis Erectile Dysfunction MicroRNAs |
topic |
Alcoholism Apoptosis Erectile Dysfunction MicroRNAs |
description |
Previous studies from our group described the consequences of using ethanol on penile erection. Nevertheless, the molecular mechanisms surrounding microRNAs, apoptosis process and their relationship with erectile dysfunction associated with alcohol consumption are still poorly understood. The objective of this analysis was to evaluate the mechanism of apoptosis by the expression of AIF and PARP, as well as their regulatory microRNAs: miR-145, miR-210 and miR-486, in the corpus cavemosum of rats submitted to a semivoltmtary alcoholism model. For this study 24 Wistar rats were divided into two groups: control (C) and treated with 20 % ethanol (A) for seven weeks. The corpus cavernosum samples were prepared for immunohistochemical analysis of AIF and PARP protein expression, and microRNAs miR-145, miR-210, miR-486 gene expression in cavernous tissue was performed by real time PCR. The immunohistochemical analysis showed little nuclear positive labeling for the protein PARP and AIF in the corpus cavernosum of control and ethanol treated animals. After analysis of miR-145, -210 and -486 microRNA expression in the 12 animals studied, no results were found with significant statistical difference between the control and alcoholized groups. The expression ofAIF and PARP and their regulatory inicroRNAs involved in apoptotic process (miR-145, miR-210 and miR-486) were not altered in the corpus cavernosum of rats submitted to semivoltmtary alcoholism. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-01 2021-06-25T12:24:03Z 2021-06-25T12:24:03Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
International Journal Of Morphology. Temuco: Soc Chilena Anatomia, v. 38, n. 6, p. 1639-1644, 2020. 0717-9502 http://hdl.handle.net/11449/209617 WOS:000585776700021 |
identifier_str_mv |
International Journal Of Morphology. Temuco: Soc Chilena Anatomia, v. 38, n. 6, p. 1639-1644, 2020. 0717-9502 WOS:000585776700021 |
url |
http://hdl.handle.net/11449/209617 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
International Journal Of Morphology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1639-1644 |
dc.publisher.none.fl_str_mv |
Soc Chilena Anatomia |
publisher.none.fl_str_mv |
Soc Chilena Anatomia |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129542460538880 |