Detection and Quantification of Nitrifying Bacteria Using Real-Time PCR

Detalhes bibliográficos
Autor(a) principal: Pereira, Engil I. P.
Data de Publicação: 2020
Outros Autores: Teixeira Filho, Marcelo C. M. [UNESP]
Tipo de documento: Capítulo de livro
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1007/978-1-4939-9790-9_13
http://hdl.handle.net/11449/197990
Resumo: Nitrification is the microbial-mediated transformation of ammonium (NH4 +) into nitrate (NO3 −). Many plant species depend on the availability of NO3 − as the main source of nitrogen (N). On the other hand, because NO3 − is highly mobile in the soil profile, its excess concentration can cause environmental pollution. Nitrification can be estimated at the process level, but with the development of molecular techniques it is also possible to estimate the abundance of nitrifying bacteria in the soil. Hence, in this chapter we describe the procedure for detection and quantification of nitrifying bacteria in soil samples using real-time quantitative polymerase chain reaction (PCR).
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spelling Detection and Quantification of Nitrifying Bacteria Using Real-Time PCRAmplification efficiencyDNA extractionNitrificationNitrifying geneNitrosomonas europaeaNitrification is the microbial-mediated transformation of ammonium (NH4 +) into nitrate (NO3 −). Many plant species depend on the availability of NO3 − as the main source of nitrogen (N). On the other hand, because NO3 − is highly mobile in the soil profile, its excess concentration can cause environmental pollution. Nitrification can be estimated at the process level, but with the development of molecular techniques it is also possible to estimate the abundance of nitrifying bacteria in the soil. Hence, in this chapter we describe the procedure for detection and quantification of nitrifying bacteria in soil samples using real-time quantitative polymerase chain reaction (PCR).School of Earth Environmental and Marine Sciences The University of Texas Rio Grande ValleyDepartment of Plant Protection Rural Engineering and Soils São Paulo State University (UNESP)Department of Plant Protection Rural Engineering and Soils São Paulo State University (UNESP)The University of Texas Rio Grande ValleyUniversidade Estadual Paulista (Unesp)Pereira, Engil I. P.Teixeira Filho, Marcelo C. M. [UNESP]2020-12-12T00:55:59Z2020-12-12T00:55:59Z2020-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/bookPart145-153http://dx.doi.org/10.1007/978-1-4939-9790-9_13Methods in Molecular Biology, v. 2057, p. 145-153.1940-60291064-3745http://hdl.handle.net/11449/19799010.1007/978-1-4939-9790-9_132-s2.0-85073051592Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMethods in Molecular Biologyinfo:eu-repo/semantics/openAccess2021-10-23T07:53:12Zoai:repositorio.unesp.br:11449/197990Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T07:53:12Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Detection and Quantification of Nitrifying Bacteria Using Real-Time PCR
title Detection and Quantification of Nitrifying Bacteria Using Real-Time PCR
spellingShingle Detection and Quantification of Nitrifying Bacteria Using Real-Time PCR
Pereira, Engil I. P.
Amplification efficiency
DNA extraction
Nitrification
Nitrifying gene
Nitrosomonas europaea
title_short Detection and Quantification of Nitrifying Bacteria Using Real-Time PCR
title_full Detection and Quantification of Nitrifying Bacteria Using Real-Time PCR
title_fullStr Detection and Quantification of Nitrifying Bacteria Using Real-Time PCR
title_full_unstemmed Detection and Quantification of Nitrifying Bacteria Using Real-Time PCR
title_sort Detection and Quantification of Nitrifying Bacteria Using Real-Time PCR
author Pereira, Engil I. P.
author_facet Pereira, Engil I. P.
Teixeira Filho, Marcelo C. M. [UNESP]
author_role author
author2 Teixeira Filho, Marcelo C. M. [UNESP]
author2_role author
dc.contributor.none.fl_str_mv The University of Texas Rio Grande Valley
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Pereira, Engil I. P.
Teixeira Filho, Marcelo C. M. [UNESP]
dc.subject.por.fl_str_mv Amplification efficiency
DNA extraction
Nitrification
Nitrifying gene
Nitrosomonas europaea
topic Amplification efficiency
DNA extraction
Nitrification
Nitrifying gene
Nitrosomonas europaea
description Nitrification is the microbial-mediated transformation of ammonium (NH4 +) into nitrate (NO3 −). Many plant species depend on the availability of NO3 − as the main source of nitrogen (N). On the other hand, because NO3 − is highly mobile in the soil profile, its excess concentration can cause environmental pollution. Nitrification can be estimated at the process level, but with the development of molecular techniques it is also possible to estimate the abundance of nitrifying bacteria in the soil. Hence, in this chapter we describe the procedure for detection and quantification of nitrifying bacteria in soil samples using real-time quantitative polymerase chain reaction (PCR).
publishDate 2020
dc.date.none.fl_str_mv 2020-12-12T00:55:59Z
2020-12-12T00:55:59Z
2020-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/bookPart
format bookPart
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1007/978-1-4939-9790-9_13
Methods in Molecular Biology, v. 2057, p. 145-153.
1940-6029
1064-3745
http://hdl.handle.net/11449/197990
10.1007/978-1-4939-9790-9_13
2-s2.0-85073051592
url http://dx.doi.org/10.1007/978-1-4939-9790-9_13
http://hdl.handle.net/11449/197990
identifier_str_mv Methods in Molecular Biology, v. 2057, p. 145-153.
1940-6029
1064-3745
10.1007/978-1-4939-9790-9_13
2-s2.0-85073051592
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Methods in Molecular Biology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 145-153
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1792961523783565312

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