Decreased collagen type i is associated with increased metalloproteinase-2 activity and protein expression of leptin in the myocardium of obese rats
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng por |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.36660/abc.20180143 http://hdl.handle.net/11449/202020 |
Resumo: | Background: Obesity is a risk factor for medical complications, including the cardiovascular system. There is limited information on collagen in the heart in obesity. Our previous study showed decreased protein levels of myocardial collagen type I in obese rats fed a high-fat diet for 34 weeks. However, the mechanisms responsible for low levels are not fully elucidated. Objective: The purpose of this study was to test the hypothesis that the reduction in collagen type I is associated with increased metalloproteinase-2 (MMP-2) activity, which is linked to elevated leptin in the myocardium of obese rats. Methods: Thirty-day-old male Wistar rats were randomized into two groups, control (standard diet) and obese (high-fat diet), and fed for 34 weeks. The general animal characteristics and metabolic and endocrine profiles were evaluated. Myocardial protein expressions of collagen I, leptin, tissue inhibitors of metalloproteinases (TIMP), and MMP-2 activity were assessed. Pearson correlation was employed to determine the associations between variables. The level of significance was 5%. Results: The obese animals had increased adiposity index compared to control. Comorbidities such as glucose intolerance, hyperinsulinemia, insulin resistance, hyperleptinemia, and hypertension were observed in obese rats. Obesity reduced collagen I, TIMP-1, and TIMP-2, and it increased leptin and MMP-2 in the myocardium. There was a negative correlation between collagen I and MMP-2 and a positive correlation between leptin and MMP-2. Conclusion: The hypothesis was confirmed; the reduction in collagen type I is associated with increased MMP-2 activity and leptin expression in the myocardium of obese rats. (Arq Bras Cardiol. 2020; 115(1):61-70). |
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Decreased collagen type i is associated with increased metalloproteinase-2 activity and protein expression of leptin in the myocardium of obese ratsA redução do colágeno tipo i está associada ao aumento da atividade da metaloproteinase-2 e da expressão proteica de leptina no miocárdio de ratos obesosAdiposityCardiovascular Diseases/physiopathologyCollagen Type 1LeptinMetaloproteinase-2ObesityRatsTissue Inhibitor of MetalloproteinasesBackground: Obesity is a risk factor for medical complications, including the cardiovascular system. There is limited information on collagen in the heart in obesity. Our previous study showed decreased protein levels of myocardial collagen type I in obese rats fed a high-fat diet for 34 weeks. However, the mechanisms responsible for low levels are not fully elucidated. Objective: The purpose of this study was to test the hypothesis that the reduction in collagen type I is associated with increased metalloproteinase-2 (MMP-2) activity, which is linked to elevated leptin in the myocardium of obese rats. Methods: Thirty-day-old male Wistar rats were randomized into two groups, control (standard diet) and obese (high-fat diet), and fed for 34 weeks. The general animal characteristics and metabolic and endocrine profiles were evaluated. Myocardial protein expressions of collagen I, leptin, tissue inhibitors of metalloproteinases (TIMP), and MMP-2 activity were assessed. Pearson correlation was employed to determine the associations between variables. The level of significance was 5%. Results: The obese animals had increased adiposity index compared to control. Comorbidities such as glucose intolerance, hyperinsulinemia, insulin resistance, hyperleptinemia, and hypertension were observed in obese rats. Obesity reduced collagen I, TIMP-1, and TIMP-2, and it increased leptin and MMP-2 in the myocardium. There was a negative correlation between collagen I and MMP-2 and a positive correlation between leptin and MMP-2. Conclusion: The hypothesis was confirmed; the reduction in collagen type I is associated with increased MMP-2 activity and leptin expression in the myocardium of obese rats. (Arq Bras Cardiol. 2020; 115(1):61-70).Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Faculdade de Medicina de Botucatu Universidade Estadual Paulista (UNESP)Instituto de Biociências-Universidade Estadual Paulista (UNESP)Faculdade de Medicina de Botucatu Universidade Estadual Paulista (UNESP)Instituto de Biociências-Universidade Estadual Paulista (UNESP)FAPESP: 2010/14208-4FAPESP: 2011/12165-9Universidade Estadual Paulista (Unesp)da Silva-Bertani, Danielle Cristina Tomaz [UNESP]Vileigas, Danielle Fernandes [UNESP]Mota, Gustavo Augusto Ferreira [UNESP]de Souza, Sérgio Luiz Borges [UNESP]De Tomasi, Loreta Casquel [UNESP]de Campos, Dijon Henrique Salomé [UNESP]de Deus, Adriana Fernandes [UNESP]Freire, Paula Paccielli [UNESP]Alves, Carlos Augusto Barnabe [UNESP]Padovani, Carlos Roberto [UNESP]Cicogna, Antonio Carlos [UNESP]2020-12-12T02:47:49Z2020-12-12T02:47:49Z2020-07-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article61-70application/pdfhttp://dx.doi.org/10.36660/abc.20180143Arquivos Brasileiros de Cardiologia, v. 115, n. 1, p. 61-70, 2020.1678-41700066-782Xhttp://hdl.handle.net/11449/20202010.36660/abc.20180143S0066-782X20200008000612-s2.0-85089407944S0066-782X2020000800061.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengporArquivos Brasileiros de Cardiologiainfo:eu-repo/semantics/openAccess2024-08-14T17:36:02Zoai:repositorio.unesp.br:11449/202020Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-14T17:36:02Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Decreased collagen type i is associated with increased metalloproteinase-2 activity and protein expression of leptin in the myocardium of obese rats A redução do colágeno tipo i está associada ao aumento da atividade da metaloproteinase-2 e da expressão proteica de leptina no miocárdio de ratos obesos |
title |
Decreased collagen type i is associated with increased metalloproteinase-2 activity and protein expression of leptin in the myocardium of obese rats |
spellingShingle |
Decreased collagen type i is associated with increased metalloproteinase-2 activity and protein expression of leptin in the myocardium of obese rats da Silva-Bertani, Danielle Cristina Tomaz [UNESP] Adiposity Cardiovascular Diseases/physiopathology Collagen Type 1 Leptin Metaloproteinase-2 Obesity Rats Tissue Inhibitor of Metalloproteinases |
title_short |
Decreased collagen type i is associated with increased metalloproteinase-2 activity and protein expression of leptin in the myocardium of obese rats |
title_full |
Decreased collagen type i is associated with increased metalloproteinase-2 activity and protein expression of leptin in the myocardium of obese rats |
title_fullStr |
Decreased collagen type i is associated with increased metalloproteinase-2 activity and protein expression of leptin in the myocardium of obese rats |
title_full_unstemmed |
Decreased collagen type i is associated with increased metalloproteinase-2 activity and protein expression of leptin in the myocardium of obese rats |
title_sort |
Decreased collagen type i is associated with increased metalloproteinase-2 activity and protein expression of leptin in the myocardium of obese rats |
author |
da Silva-Bertani, Danielle Cristina Tomaz [UNESP] |
author_facet |
da Silva-Bertani, Danielle Cristina Tomaz [UNESP] Vileigas, Danielle Fernandes [UNESP] Mota, Gustavo Augusto Ferreira [UNESP] de Souza, Sérgio Luiz Borges [UNESP] De Tomasi, Loreta Casquel [UNESP] de Campos, Dijon Henrique Salomé [UNESP] de Deus, Adriana Fernandes [UNESP] Freire, Paula Paccielli [UNESP] Alves, Carlos Augusto Barnabe [UNESP] Padovani, Carlos Roberto [UNESP] Cicogna, Antonio Carlos [UNESP] |
author_role |
author |
author2 |
Vileigas, Danielle Fernandes [UNESP] Mota, Gustavo Augusto Ferreira [UNESP] de Souza, Sérgio Luiz Borges [UNESP] De Tomasi, Loreta Casquel [UNESP] de Campos, Dijon Henrique Salomé [UNESP] de Deus, Adriana Fernandes [UNESP] Freire, Paula Paccielli [UNESP] Alves, Carlos Augusto Barnabe [UNESP] Padovani, Carlos Roberto [UNESP] Cicogna, Antonio Carlos [UNESP] |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
da Silva-Bertani, Danielle Cristina Tomaz [UNESP] Vileigas, Danielle Fernandes [UNESP] Mota, Gustavo Augusto Ferreira [UNESP] de Souza, Sérgio Luiz Borges [UNESP] De Tomasi, Loreta Casquel [UNESP] de Campos, Dijon Henrique Salomé [UNESP] de Deus, Adriana Fernandes [UNESP] Freire, Paula Paccielli [UNESP] Alves, Carlos Augusto Barnabe [UNESP] Padovani, Carlos Roberto [UNESP] Cicogna, Antonio Carlos [UNESP] |
dc.subject.por.fl_str_mv |
Adiposity Cardiovascular Diseases/physiopathology Collagen Type 1 Leptin Metaloproteinase-2 Obesity Rats Tissue Inhibitor of Metalloproteinases |
topic |
Adiposity Cardiovascular Diseases/physiopathology Collagen Type 1 Leptin Metaloproteinase-2 Obesity Rats Tissue Inhibitor of Metalloproteinases |
description |
Background: Obesity is a risk factor for medical complications, including the cardiovascular system. There is limited information on collagen in the heart in obesity. Our previous study showed decreased protein levels of myocardial collagen type I in obese rats fed a high-fat diet for 34 weeks. However, the mechanisms responsible for low levels are not fully elucidated. Objective: The purpose of this study was to test the hypothesis that the reduction in collagen type I is associated with increased metalloproteinase-2 (MMP-2) activity, which is linked to elevated leptin in the myocardium of obese rats. Methods: Thirty-day-old male Wistar rats were randomized into two groups, control (standard diet) and obese (high-fat diet), and fed for 34 weeks. The general animal characteristics and metabolic and endocrine profiles were evaluated. Myocardial protein expressions of collagen I, leptin, tissue inhibitors of metalloproteinases (TIMP), and MMP-2 activity were assessed. Pearson correlation was employed to determine the associations between variables. The level of significance was 5%. Results: The obese animals had increased adiposity index compared to control. Comorbidities such as glucose intolerance, hyperinsulinemia, insulin resistance, hyperleptinemia, and hypertension were observed in obese rats. Obesity reduced collagen I, TIMP-1, and TIMP-2, and it increased leptin and MMP-2 in the myocardium. There was a negative correlation between collagen I and MMP-2 and a positive correlation between leptin and MMP-2. Conclusion: The hypothesis was confirmed; the reduction in collagen type I is associated with increased MMP-2 activity and leptin expression in the myocardium of obese rats. (Arq Bras Cardiol. 2020; 115(1):61-70). |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T02:47:49Z 2020-12-12T02:47:49Z 2020-07-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.36660/abc.20180143 Arquivos Brasileiros de Cardiologia, v. 115, n. 1, p. 61-70, 2020. 1678-4170 0066-782X http://hdl.handle.net/11449/202020 10.36660/abc.20180143 S0066-782X2020000800061 2-s2.0-85089407944 S0066-782X2020000800061.pdf |
url |
http://dx.doi.org/10.36660/abc.20180143 http://hdl.handle.net/11449/202020 |
identifier_str_mv |
Arquivos Brasileiros de Cardiologia, v. 115, n. 1, p. 61-70, 2020. 1678-4170 0066-782X 10.36660/abc.20180143 S0066-782X2020000800061 2-s2.0-85089407944 S0066-782X2020000800061.pdf |
dc.language.iso.fl_str_mv |
eng por |
language |
eng por |
dc.relation.none.fl_str_mv |
Arquivos Brasileiros de Cardiologia |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
61-70 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808128179241484288 |