Putative progressive and abortive feline leukemia virus infection outcomes in captive jaguarundis (Puma yagouaroundi)

Detalhes bibliográficos
Autor(a) principal: Filoni, Claudia [UNESP]
Data de Publicação: 2017
Outros Autores: Helfer-Hungerbuehler, A. Katrin, Catão-Dias, José Luiz, Marques, Mara Cristina, Torres, Luciana Neves, Reinacher, Manfred, Hofmann-Lehmann, Regina
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1186/s12985-017-0889-z
http://hdl.handle.net/11449/175505
Resumo: Background: Feline leukemia virus (FeLV) is an exogenous gammaretrovirus of domestic cats (Felis catus) and some wild felids. The outcomes of FeLV infection in domestic cats vary according to host susceptibility, virus strain, and infectious challenge dose. Jaguarundis (Puma yagouaroundi) are small wild felids from South and Central America. We previously reported on FeLV infections in jaguarundis. We hypothesized here that the outcomes of FeLV infection in P. yagouaroundi mimic those observed in domestic cats. The aim of this study was to investigate the population of jaguarundis at Fundação Parque Zoológico de São Paulo for natural FeLV infection and resulting outcomes. Methods: We investigated the jaguarundis using serological and molecular methods and monitored them for FeLV-related diseases for 5 years. We retrieved relevant biological and clinical information for the entire population of 23 jaguarundis held at zoo. Post-mortem findings from necropsies were recorded and histopathological and immunohistopathological analyses were performed. Sequencing and phylogenetic analyses were performed for FeLV-positive samples. For sample prevalence, 95% confidence intervals (CI) were calculated. Fisher's exact test was used to compare frequencies between infected and uninfected animals. P-values <0.05 were considered significant. Results: In total, we detected evidence of FeLV exposure in four out of 23 animals (17%; 95% CI 5-39%). No endogenous FeLV (enFeLV) sequences were detected. An intestinal B-cell lymphoma in one jaguarundi was not associated with FeLV. Two jaguarundis presented FeLV test results consistent with an abortive FeLV infection with seroconversion, and two other jaguarundis had results consistent with a progressive infection and potentially FeLV-associated clinical disorders and post-mortem changes. Phylogenetic analysis of env revealed the presence of FeLV-A, a common origin of the virus in both animals (100% identity) and the closest similarity to FeLV-FAIDS and FeLV-3281 (98.4% identity), originally isolated from cats in the USA. Conclusions: We found evidence of progressive and abortive FeLV infection outcomes in jaguarundis, and domestic cats were probably the source of infection in these jaguarundis.
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spelling Putative progressive and abortive feline leukemia virus infection outcomes in captive jaguarundis (Puma yagouaroundi)enFeLVFeLV-AIntestinal B-cell lymphomaqPCRRetrovirusRT-qPCRWild felidsBackground: Feline leukemia virus (FeLV) is an exogenous gammaretrovirus of domestic cats (Felis catus) and some wild felids. The outcomes of FeLV infection in domestic cats vary according to host susceptibility, virus strain, and infectious challenge dose. Jaguarundis (Puma yagouaroundi) are small wild felids from South and Central America. We previously reported on FeLV infections in jaguarundis. We hypothesized here that the outcomes of FeLV infection in P. yagouaroundi mimic those observed in domestic cats. The aim of this study was to investigate the population of jaguarundis at Fundação Parque Zoológico de São Paulo for natural FeLV infection and resulting outcomes. Methods: We investigated the jaguarundis using serological and molecular methods and monitored them for FeLV-related diseases for 5 years. We retrieved relevant biological and clinical information for the entire population of 23 jaguarundis held at zoo. Post-mortem findings from necropsies were recorded and histopathological and immunohistopathological analyses were performed. Sequencing and phylogenetic analyses were performed for FeLV-positive samples. For sample prevalence, 95% confidence intervals (CI) were calculated. Fisher's exact test was used to compare frequencies between infected and uninfected animals. P-values <0.05 were considered significant. Results: In total, we detected evidence of FeLV exposure in four out of 23 animals (17%; 95% CI 5-39%). No endogenous FeLV (enFeLV) sequences were detected. An intestinal B-cell lymphoma in one jaguarundi was not associated with FeLV. Two jaguarundis presented FeLV test results consistent with an abortive FeLV infection with seroconversion, and two other jaguarundis had results consistent with a progressive infection and potentially FeLV-associated clinical disorders and post-mortem changes. Phylogenetic analysis of env revealed the presence of FeLV-A, a common origin of the virus in both animals (100% identity) and the closest similarity to FeLV-FAIDS and FeLV-3281 (98.4% identity), originally isolated from cats in the USA. Conclusions: We found evidence of progressive and abortive FeLV infection outcomes in jaguarundis, and domestic cats were probably the source of infection in these jaguarundis.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Institute of Biosciences Department of Microbiology and Immunology São Paulo State University (UNESP) Campus Botucatu, Rua Professor Doutor Antonio Celso Wagner Zanin SNVetsuisse Faculty Clinical Laboratory Center for Clinical Studies University of Zurich, Winterthurerstrasse 260School of Veterinary Medicine and Animal Sciences Department of Pathology University of São Paulo (USP), Av. Prof. Orlando Marques de Paiva 87, São PauloFundação Parque Zoológico de São Paulo (FPZSP), Av. Miguel Stéfano 4241, São PauloSchool of Veterinary Medicine and Animal Sciences Veterinary Hospital (HOVET) University of São Paulo (USP), Av. Prof. Orlando Marques de Paiva 87, São PauloInstitute of Veterinary Pathology University of Giessen, Frankfurter Strasse 96Institute of Biosciences Department of Microbiology and Immunology São Paulo State University (UNESP) Campus Botucatu, Rua Professor Doutor Antonio Celso Wagner Zanin SNUniversidade Estadual Paulista (Unesp)University of ZurichUniversidade de São Paulo (USP)Fundação Parque Zoológico de São Paulo (FPZSP)University of GiessenFiloni, Claudia [UNESP]Helfer-Hungerbuehler, A. KatrinCatão-Dias, José LuizMarques, Mara CristinaTorres, Luciana NevesReinacher, ManfredHofmann-Lehmann, Regina2018-12-11T17:16:05Z2018-12-11T17:16:05Z2017-11-17info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1186/s12985-017-0889-zVirology Journal, v. 14, n. 1, 2017.1743-422Xhttp://hdl.handle.net/11449/17550510.1186/s12985-017-0889-z2-s2.0-850344457422-s2.0-85034445742.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengVirology Journalinfo:eu-repo/semantics/openAccess2023-11-02T06:11:39Zoai:repositorio.unesp.br:11449/175505Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:44:40.864633Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Putative progressive and abortive feline leukemia virus infection outcomes in captive jaguarundis (Puma yagouaroundi)
title Putative progressive and abortive feline leukemia virus infection outcomes in captive jaguarundis (Puma yagouaroundi)
spellingShingle Putative progressive and abortive feline leukemia virus infection outcomes in captive jaguarundis (Puma yagouaroundi)
Filoni, Claudia [UNESP]
enFeLV
FeLV-A
Intestinal B-cell lymphoma
qPCR
Retrovirus
RT-qPCR
Wild felids
title_short Putative progressive and abortive feline leukemia virus infection outcomes in captive jaguarundis (Puma yagouaroundi)
title_full Putative progressive and abortive feline leukemia virus infection outcomes in captive jaguarundis (Puma yagouaroundi)
title_fullStr Putative progressive and abortive feline leukemia virus infection outcomes in captive jaguarundis (Puma yagouaroundi)
title_full_unstemmed Putative progressive and abortive feline leukemia virus infection outcomes in captive jaguarundis (Puma yagouaroundi)
title_sort Putative progressive and abortive feline leukemia virus infection outcomes in captive jaguarundis (Puma yagouaroundi)
author Filoni, Claudia [UNESP]
author_facet Filoni, Claudia [UNESP]
Helfer-Hungerbuehler, A. Katrin
Catão-Dias, José Luiz
Marques, Mara Cristina
Torres, Luciana Neves
Reinacher, Manfred
Hofmann-Lehmann, Regina
author_role author
author2 Helfer-Hungerbuehler, A. Katrin
Catão-Dias, José Luiz
Marques, Mara Cristina
Torres, Luciana Neves
Reinacher, Manfred
Hofmann-Lehmann, Regina
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
University of Zurich
Universidade de São Paulo (USP)
Fundação Parque Zoológico de São Paulo (FPZSP)
University of Giessen
dc.contributor.author.fl_str_mv Filoni, Claudia [UNESP]
Helfer-Hungerbuehler, A. Katrin
Catão-Dias, José Luiz
Marques, Mara Cristina
Torres, Luciana Neves
Reinacher, Manfred
Hofmann-Lehmann, Regina
dc.subject.por.fl_str_mv enFeLV
FeLV-A
Intestinal B-cell lymphoma
qPCR
Retrovirus
RT-qPCR
Wild felids
topic enFeLV
FeLV-A
Intestinal B-cell lymphoma
qPCR
Retrovirus
RT-qPCR
Wild felids
description Background: Feline leukemia virus (FeLV) is an exogenous gammaretrovirus of domestic cats (Felis catus) and some wild felids. The outcomes of FeLV infection in domestic cats vary according to host susceptibility, virus strain, and infectious challenge dose. Jaguarundis (Puma yagouaroundi) are small wild felids from South and Central America. We previously reported on FeLV infections in jaguarundis. We hypothesized here that the outcomes of FeLV infection in P. yagouaroundi mimic those observed in domestic cats. The aim of this study was to investigate the population of jaguarundis at Fundação Parque Zoológico de São Paulo for natural FeLV infection and resulting outcomes. Methods: We investigated the jaguarundis using serological and molecular methods and monitored them for FeLV-related diseases for 5 years. We retrieved relevant biological and clinical information for the entire population of 23 jaguarundis held at zoo. Post-mortem findings from necropsies were recorded and histopathological and immunohistopathological analyses were performed. Sequencing and phylogenetic analyses were performed for FeLV-positive samples. For sample prevalence, 95% confidence intervals (CI) were calculated. Fisher's exact test was used to compare frequencies between infected and uninfected animals. P-values <0.05 were considered significant. Results: In total, we detected evidence of FeLV exposure in four out of 23 animals (17%; 95% CI 5-39%). No endogenous FeLV (enFeLV) sequences were detected. An intestinal B-cell lymphoma in one jaguarundi was not associated with FeLV. Two jaguarundis presented FeLV test results consistent with an abortive FeLV infection with seroconversion, and two other jaguarundis had results consistent with a progressive infection and potentially FeLV-associated clinical disorders and post-mortem changes. Phylogenetic analysis of env revealed the presence of FeLV-A, a common origin of the virus in both animals (100% identity) and the closest similarity to FeLV-FAIDS and FeLV-3281 (98.4% identity), originally isolated from cats in the USA. Conclusions: We found evidence of progressive and abortive FeLV infection outcomes in jaguarundis, and domestic cats were probably the source of infection in these jaguarundis.
publishDate 2017
dc.date.none.fl_str_mv 2017-11-17
2018-12-11T17:16:05Z
2018-12-11T17:16:05Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1186/s12985-017-0889-z
Virology Journal, v. 14, n. 1, 2017.
1743-422X
http://hdl.handle.net/11449/175505
10.1186/s12985-017-0889-z
2-s2.0-85034445742
2-s2.0-85034445742.pdf
url http://dx.doi.org/10.1186/s12985-017-0889-z
http://hdl.handle.net/11449/175505
identifier_str_mv Virology Journal, v. 14, n. 1, 2017.
1743-422X
10.1186/s12985-017-0889-z
2-s2.0-85034445742
2-s2.0-85034445742.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Virology Journal
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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