Putative progressive and abortive feline leukemia virus infection outcomes in captive jaguarundis (Puma yagouaroundi)
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1186/s12985-017-0889-z http://hdl.handle.net/11449/175505 |
Resumo: | Background: Feline leukemia virus (FeLV) is an exogenous gammaretrovirus of domestic cats (Felis catus) and some wild felids. The outcomes of FeLV infection in domestic cats vary according to host susceptibility, virus strain, and infectious challenge dose. Jaguarundis (Puma yagouaroundi) are small wild felids from South and Central America. We previously reported on FeLV infections in jaguarundis. We hypothesized here that the outcomes of FeLV infection in P. yagouaroundi mimic those observed in domestic cats. The aim of this study was to investigate the population of jaguarundis at Fundação Parque Zoológico de São Paulo for natural FeLV infection and resulting outcomes. Methods: We investigated the jaguarundis using serological and molecular methods and monitored them for FeLV-related diseases for 5 years. We retrieved relevant biological and clinical information for the entire population of 23 jaguarundis held at zoo. Post-mortem findings from necropsies were recorded and histopathological and immunohistopathological analyses were performed. Sequencing and phylogenetic analyses were performed for FeLV-positive samples. For sample prevalence, 95% confidence intervals (CI) were calculated. Fisher's exact test was used to compare frequencies between infected and uninfected animals. P-values <0.05 were considered significant. Results: In total, we detected evidence of FeLV exposure in four out of 23 animals (17%; 95% CI 5-39%). No endogenous FeLV (enFeLV) sequences were detected. An intestinal B-cell lymphoma in one jaguarundi was not associated with FeLV. Two jaguarundis presented FeLV test results consistent with an abortive FeLV infection with seroconversion, and two other jaguarundis had results consistent with a progressive infection and potentially FeLV-associated clinical disorders and post-mortem changes. Phylogenetic analysis of env revealed the presence of FeLV-A, a common origin of the virus in both animals (100% identity) and the closest similarity to FeLV-FAIDS and FeLV-3281 (98.4% identity), originally isolated from cats in the USA. Conclusions: We found evidence of progressive and abortive FeLV infection outcomes in jaguarundis, and domestic cats were probably the source of infection in these jaguarundis. |
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Putative progressive and abortive feline leukemia virus infection outcomes in captive jaguarundis (Puma yagouaroundi)enFeLVFeLV-AIntestinal B-cell lymphomaqPCRRetrovirusRT-qPCRWild felidsBackground: Feline leukemia virus (FeLV) is an exogenous gammaretrovirus of domestic cats (Felis catus) and some wild felids. The outcomes of FeLV infection in domestic cats vary according to host susceptibility, virus strain, and infectious challenge dose. Jaguarundis (Puma yagouaroundi) are small wild felids from South and Central America. We previously reported on FeLV infections in jaguarundis. We hypothesized here that the outcomes of FeLV infection in P. yagouaroundi mimic those observed in domestic cats. The aim of this study was to investigate the population of jaguarundis at Fundação Parque Zoológico de São Paulo for natural FeLV infection and resulting outcomes. Methods: We investigated the jaguarundis using serological and molecular methods and monitored them for FeLV-related diseases for 5 years. We retrieved relevant biological and clinical information for the entire population of 23 jaguarundis held at zoo. Post-mortem findings from necropsies were recorded and histopathological and immunohistopathological analyses were performed. Sequencing and phylogenetic analyses were performed for FeLV-positive samples. For sample prevalence, 95% confidence intervals (CI) were calculated. Fisher's exact test was used to compare frequencies between infected and uninfected animals. P-values <0.05 were considered significant. Results: In total, we detected evidence of FeLV exposure in four out of 23 animals (17%; 95% CI 5-39%). No endogenous FeLV (enFeLV) sequences were detected. An intestinal B-cell lymphoma in one jaguarundi was not associated with FeLV. Two jaguarundis presented FeLV test results consistent with an abortive FeLV infection with seroconversion, and two other jaguarundis had results consistent with a progressive infection and potentially FeLV-associated clinical disorders and post-mortem changes. Phylogenetic analysis of env revealed the presence of FeLV-A, a common origin of the virus in both animals (100% identity) and the closest similarity to FeLV-FAIDS and FeLV-3281 (98.4% identity), originally isolated from cats in the USA. Conclusions: We found evidence of progressive and abortive FeLV infection outcomes in jaguarundis, and domestic cats were probably the source of infection in these jaguarundis.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Institute of Biosciences Department of Microbiology and Immunology São Paulo State University (UNESP) Campus Botucatu, Rua Professor Doutor Antonio Celso Wagner Zanin SNVetsuisse Faculty Clinical Laboratory Center for Clinical Studies University of Zurich, Winterthurerstrasse 260School of Veterinary Medicine and Animal Sciences Department of Pathology University of São Paulo (USP), Av. Prof. Orlando Marques de Paiva 87, São PauloFundação Parque Zoológico de São Paulo (FPZSP), Av. Miguel Stéfano 4241, São PauloSchool of Veterinary Medicine and Animal Sciences Veterinary Hospital (HOVET) University of São Paulo (USP), Av. Prof. Orlando Marques de Paiva 87, São PauloInstitute of Veterinary Pathology University of Giessen, Frankfurter Strasse 96Institute of Biosciences Department of Microbiology and Immunology São Paulo State University (UNESP) Campus Botucatu, Rua Professor Doutor Antonio Celso Wagner Zanin SNUniversidade Estadual Paulista (Unesp)University of ZurichUniversidade de São Paulo (USP)Fundação Parque Zoológico de São Paulo (FPZSP)University of GiessenFiloni, Claudia [UNESP]Helfer-Hungerbuehler, A. KatrinCatão-Dias, José LuizMarques, Mara CristinaTorres, Luciana NevesReinacher, ManfredHofmann-Lehmann, Regina2018-12-11T17:16:05Z2018-12-11T17:16:05Z2017-11-17info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1186/s12985-017-0889-zVirology Journal, v. 14, n. 1, 2017.1743-422Xhttp://hdl.handle.net/11449/17550510.1186/s12985-017-0889-z2-s2.0-850344457422-s2.0-85034445742.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengVirology Journalinfo:eu-repo/semantics/openAccess2023-11-02T06:11:39Zoai:repositorio.unesp.br:11449/175505Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:44:40.864633Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Putative progressive and abortive feline leukemia virus infection outcomes in captive jaguarundis (Puma yagouaroundi) |
title |
Putative progressive and abortive feline leukemia virus infection outcomes in captive jaguarundis (Puma yagouaroundi) |
spellingShingle |
Putative progressive and abortive feline leukemia virus infection outcomes in captive jaguarundis (Puma yagouaroundi) Filoni, Claudia [UNESP] enFeLV FeLV-A Intestinal B-cell lymphoma qPCR Retrovirus RT-qPCR Wild felids |
title_short |
Putative progressive and abortive feline leukemia virus infection outcomes in captive jaguarundis (Puma yagouaroundi) |
title_full |
Putative progressive and abortive feline leukemia virus infection outcomes in captive jaguarundis (Puma yagouaroundi) |
title_fullStr |
Putative progressive and abortive feline leukemia virus infection outcomes in captive jaguarundis (Puma yagouaroundi) |
title_full_unstemmed |
Putative progressive and abortive feline leukemia virus infection outcomes in captive jaguarundis (Puma yagouaroundi) |
title_sort |
Putative progressive and abortive feline leukemia virus infection outcomes in captive jaguarundis (Puma yagouaroundi) |
author |
Filoni, Claudia [UNESP] |
author_facet |
Filoni, Claudia [UNESP] Helfer-Hungerbuehler, A. Katrin Catão-Dias, José Luiz Marques, Mara Cristina Torres, Luciana Neves Reinacher, Manfred Hofmann-Lehmann, Regina |
author_role |
author |
author2 |
Helfer-Hungerbuehler, A. Katrin Catão-Dias, José Luiz Marques, Mara Cristina Torres, Luciana Neves Reinacher, Manfred Hofmann-Lehmann, Regina |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) University of Zurich Universidade de São Paulo (USP) Fundação Parque Zoológico de São Paulo (FPZSP) University of Giessen |
dc.contributor.author.fl_str_mv |
Filoni, Claudia [UNESP] Helfer-Hungerbuehler, A. Katrin Catão-Dias, José Luiz Marques, Mara Cristina Torres, Luciana Neves Reinacher, Manfred Hofmann-Lehmann, Regina |
dc.subject.por.fl_str_mv |
enFeLV FeLV-A Intestinal B-cell lymphoma qPCR Retrovirus RT-qPCR Wild felids |
topic |
enFeLV FeLV-A Intestinal B-cell lymphoma qPCR Retrovirus RT-qPCR Wild felids |
description |
Background: Feline leukemia virus (FeLV) is an exogenous gammaretrovirus of domestic cats (Felis catus) and some wild felids. The outcomes of FeLV infection in domestic cats vary according to host susceptibility, virus strain, and infectious challenge dose. Jaguarundis (Puma yagouaroundi) are small wild felids from South and Central America. We previously reported on FeLV infections in jaguarundis. We hypothesized here that the outcomes of FeLV infection in P. yagouaroundi mimic those observed in domestic cats. The aim of this study was to investigate the population of jaguarundis at Fundação Parque Zoológico de São Paulo for natural FeLV infection and resulting outcomes. Methods: We investigated the jaguarundis using serological and molecular methods and monitored them for FeLV-related diseases for 5 years. We retrieved relevant biological and clinical information for the entire population of 23 jaguarundis held at zoo. Post-mortem findings from necropsies were recorded and histopathological and immunohistopathological analyses were performed. Sequencing and phylogenetic analyses were performed for FeLV-positive samples. For sample prevalence, 95% confidence intervals (CI) were calculated. Fisher's exact test was used to compare frequencies between infected and uninfected animals. P-values <0.05 were considered significant. Results: In total, we detected evidence of FeLV exposure in four out of 23 animals (17%; 95% CI 5-39%). No endogenous FeLV (enFeLV) sequences were detected. An intestinal B-cell lymphoma in one jaguarundi was not associated with FeLV. Two jaguarundis presented FeLV test results consistent with an abortive FeLV infection with seroconversion, and two other jaguarundis had results consistent with a progressive infection and potentially FeLV-associated clinical disorders and post-mortem changes. Phylogenetic analysis of env revealed the presence of FeLV-A, a common origin of the virus in both animals (100% identity) and the closest similarity to FeLV-FAIDS and FeLV-3281 (98.4% identity), originally isolated from cats in the USA. Conclusions: We found evidence of progressive and abortive FeLV infection outcomes in jaguarundis, and domestic cats were probably the source of infection in these jaguarundis. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-11-17 2018-12-11T17:16:05Z 2018-12-11T17:16:05Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1186/s12985-017-0889-z Virology Journal, v. 14, n. 1, 2017. 1743-422X http://hdl.handle.net/11449/175505 10.1186/s12985-017-0889-z 2-s2.0-85034445742 2-s2.0-85034445742.pdf |
url |
http://dx.doi.org/10.1186/s12985-017-0889-z http://hdl.handle.net/11449/175505 |
identifier_str_mv |
Virology Journal, v. 14, n. 1, 2017. 1743-422X 10.1186/s12985-017-0889-z 2-s2.0-85034445742 2-s2.0-85034445742.pdf |
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eng |
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eng |
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Virology Journal |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
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Universidade Estadual Paulista (UNESP) |
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UNESP |
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Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
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