Changes in the oxidative stress biomarkers in liver of streptozotocin-diabetic rats treated with combretum lanceolatum flowers extract

Detalhes bibliográficos
Autor(a) principal: Dechandt, Carlos Roberto Porto
Data de Publicação: 2014
Outros Autores: Souza, Damiana Luiza Pereira, Siqueira, Juliany Torres, Pereira, Mayara Peron, Assis, Renata Pires de [UNESP], Silva, Virginia Claudia da, Sousa Junior, Paulo Teixeira de, Brunetti, Iguatemy Lourenço [UNESP], Andrade, Claudia Marlise, Kawashita, Nair Honda, Baviera, Amanda Martins [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.9734/bjpr/2014/13558
http://hdl.handle.net/11449/133702
Resumo: Aims: The study investigated the in vivo antioxidant activity and the in vitro radical scavenging capacity of the Combretum lanceolatum Pohl (Combretaceae) flowers ethanolic extract (ClEtOH) in streptozotocin-diabetic rats. Place and Duration of Study: Department of Chemistry, Federal University of Mato Grosso, Cuiabá, Brazil; between February 2012 and December 2012. Methodology: Male Wistar rats were divided into four groups: Normal rats treated with water/vehicle (N); diabetic rats treated with water (DC); diabetic rats treated with 250 mg/kg (DT250) or with 500mg/kg (DT500) of ClEtOH. After 21 days of treatment, liver samples were used for the analysis of the oxidative stress biomarkers and activity of antioxidant enzymes. In vitro radical scavenger capacity was investigated by the following methods: DPPH radical scavenging, ABTS radical cation decolorization and crocin bleaching assays. Results: Significant oxidative stress was observed in liver of DC, since the malondialdehyde (MDA, biomarker of lipoperoxidation) levels were increased in comparison with N. Increased activities of the antioxidant enzymes superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) were also observed in DC, which could represent a compensatory mechanism against oxidative stress. Glutathione (GSH) levels were lower and similar between N and DC. The MDA levels were significantly decreased in liver of rats from DT250 and DT500, reaching levels similar those of N, suggesting that ClEtOH prevented lipoperoxidation. The treatment of diabetic rats with ClEtOH also increased the GSH levels, as well as increased the GSH-Px activity, and did not change the SOD activity. The results of in vitro radical scavenging capacity indicated that ClEtOH is highly active. Conclusion: These findings indicate that ClEtOH has antioxidant properties in liver of diabetic rats, decreasing lipoperoxidation and increasing the endogenous antioxidant responses. Both the antihyperglycemic effect and the capacity to scavenge free radicals may be related to the antioxidant activity of ClEtOH in diabetes.
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spelling Changes in the oxidative stress biomarkers in liver of streptozotocin-diabetic rats treated with combretum lanceolatum flowers extractCombretum lanceolatum PohlStreptozotocin-diabetic ratsMalondialdehydeReduced glutathioneAntioxidant enzymesAims: The study investigated the in vivo antioxidant activity and the in vitro radical scavenging capacity of the Combretum lanceolatum Pohl (Combretaceae) flowers ethanolic extract (ClEtOH) in streptozotocin-diabetic rats. Place and Duration of Study: Department of Chemistry, Federal University of Mato Grosso, Cuiabá, Brazil; between February 2012 and December 2012. Methodology: Male Wistar rats were divided into four groups: Normal rats treated with water/vehicle (N); diabetic rats treated with water (DC); diabetic rats treated with 250 mg/kg (DT250) or with 500mg/kg (DT500) of ClEtOH. After 21 days of treatment, liver samples were used for the analysis of the oxidative stress biomarkers and activity of antioxidant enzymes. In vitro radical scavenger capacity was investigated by the following methods: DPPH radical scavenging, ABTS radical cation decolorization and crocin bleaching assays. Results: Significant oxidative stress was observed in liver of DC, since the malondialdehyde (MDA, biomarker of lipoperoxidation) levels were increased in comparison with N. Increased activities of the antioxidant enzymes superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) were also observed in DC, which could represent a compensatory mechanism against oxidative stress. Glutathione (GSH) levels were lower and similar between N and DC. The MDA levels were significantly decreased in liver of rats from DT250 and DT500, reaching levels similar those of N, suggesting that ClEtOH prevented lipoperoxidation. The treatment of diabetic rats with ClEtOH also increased the GSH levels, as well as increased the GSH-Px activity, and did not change the SOD activity. The results of in vitro radical scavenging capacity indicated that ClEtOH is highly active. Conclusion: These findings indicate that ClEtOH has antioxidant properties in liver of diabetic rats, decreasing lipoperoxidation and increasing the endogenous antioxidant responses. Both the antihyperglycemic effect and the capacity to scavenge free radicals may be related to the antioxidant activity of ClEtOH in diabetes.Universidade Estadual Paulista Júlio de Mesquita Filho, Departamento de Análises Clínicas, Faculdade de Ciências Farmacêuticas de Araraquara, Araraquara, Rua Expedicionários do Brasil, 1621, Centro, CEP 14801-902, SP, BrasilUniversidade Estadual Paulista Júlio de Mesquita Filho, Departamento de Análises Clínicas, Faculdade de Ciências Farmacêuticas de Araraquara, Araraquara, Rua Expedicionários do Brasil, 1621, Centro, CEP 14801-902, SP, BrasilUniversidade Federal do Mato Grosso [UFMT]Universidade Estadual Paulista (Unesp)Dechandt, Carlos Roberto PortoSouza, Damiana Luiza PereiraSiqueira, Juliany TorresPereira, Mayara PeronAssis, Renata Pires de [UNESP]Silva, Virginia Claudia daSousa Junior, Paulo Teixeira deBrunetti, Iguatemy Lourenço [UNESP]Andrade, Claudia MarliseKawashita, Nair HondaBaviera, Amanda Martins [UNESP]2016-01-28T16:56:15Z2016-01-28T16:56:15Z2014info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2340-2356application/pdfhttp://dx.doi.org/10.9734/bjpr/2014/13558British Journal of Pharmaceutical Research, v. 4, p. 2340-2356, 2014.2231-2919http://hdl.handle.net/11449/13370210.9734/bjpr/2014/13558ISSN2231-2919-2014-04-2340-2356.pdf37364750251877501624929129014716Currículo Lattesreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBritish Journal of Pharmaceutical Researchinfo:eu-repo/semantics/openAccess2024-06-21T15:19:21Zoai:repositorio.unesp.br:11449/133702Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:15:26.865920Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Changes in the oxidative stress biomarkers in liver of streptozotocin-diabetic rats treated with combretum lanceolatum flowers extract
title Changes in the oxidative stress biomarkers in liver of streptozotocin-diabetic rats treated with combretum lanceolatum flowers extract
spellingShingle Changes in the oxidative stress biomarkers in liver of streptozotocin-diabetic rats treated with combretum lanceolatum flowers extract
Dechandt, Carlos Roberto Porto
Combretum lanceolatum Pohl
Streptozotocin-diabetic rats
Malondialdehyde
Reduced glutathione
Antioxidant enzymes
title_short Changes in the oxidative stress biomarkers in liver of streptozotocin-diabetic rats treated with combretum lanceolatum flowers extract
title_full Changes in the oxidative stress biomarkers in liver of streptozotocin-diabetic rats treated with combretum lanceolatum flowers extract
title_fullStr Changes in the oxidative stress biomarkers in liver of streptozotocin-diabetic rats treated with combretum lanceolatum flowers extract
title_full_unstemmed Changes in the oxidative stress biomarkers in liver of streptozotocin-diabetic rats treated with combretum lanceolatum flowers extract
title_sort Changes in the oxidative stress biomarkers in liver of streptozotocin-diabetic rats treated with combretum lanceolatum flowers extract
author Dechandt, Carlos Roberto Porto
author_facet Dechandt, Carlos Roberto Porto
Souza, Damiana Luiza Pereira
Siqueira, Juliany Torres
Pereira, Mayara Peron
Assis, Renata Pires de [UNESP]
Silva, Virginia Claudia da
Sousa Junior, Paulo Teixeira de
Brunetti, Iguatemy Lourenço [UNESP]
Andrade, Claudia Marlise
Kawashita, Nair Honda
Baviera, Amanda Martins [UNESP]
author_role author
author2 Souza, Damiana Luiza Pereira
Siqueira, Juliany Torres
Pereira, Mayara Peron
Assis, Renata Pires de [UNESP]
Silva, Virginia Claudia da
Sousa Junior, Paulo Teixeira de
Brunetti, Iguatemy Lourenço [UNESP]
Andrade, Claudia Marlise
Kawashita, Nair Honda
Baviera, Amanda Martins [UNESP]
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Federal do Mato Grosso [UFMT]
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Dechandt, Carlos Roberto Porto
Souza, Damiana Luiza Pereira
Siqueira, Juliany Torres
Pereira, Mayara Peron
Assis, Renata Pires de [UNESP]
Silva, Virginia Claudia da
Sousa Junior, Paulo Teixeira de
Brunetti, Iguatemy Lourenço [UNESP]
Andrade, Claudia Marlise
Kawashita, Nair Honda
Baviera, Amanda Martins [UNESP]
dc.subject.por.fl_str_mv Combretum lanceolatum Pohl
Streptozotocin-diabetic rats
Malondialdehyde
Reduced glutathione
Antioxidant enzymes
topic Combretum lanceolatum Pohl
Streptozotocin-diabetic rats
Malondialdehyde
Reduced glutathione
Antioxidant enzymes
description Aims: The study investigated the in vivo antioxidant activity and the in vitro radical scavenging capacity of the Combretum lanceolatum Pohl (Combretaceae) flowers ethanolic extract (ClEtOH) in streptozotocin-diabetic rats. Place and Duration of Study: Department of Chemistry, Federal University of Mato Grosso, Cuiabá, Brazil; between February 2012 and December 2012. Methodology: Male Wistar rats were divided into four groups: Normal rats treated with water/vehicle (N); diabetic rats treated with water (DC); diabetic rats treated with 250 mg/kg (DT250) or with 500mg/kg (DT500) of ClEtOH. After 21 days of treatment, liver samples were used for the analysis of the oxidative stress biomarkers and activity of antioxidant enzymes. In vitro radical scavenger capacity was investigated by the following methods: DPPH radical scavenging, ABTS radical cation decolorization and crocin bleaching assays. Results: Significant oxidative stress was observed in liver of DC, since the malondialdehyde (MDA, biomarker of lipoperoxidation) levels were increased in comparison with N. Increased activities of the antioxidant enzymes superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) were also observed in DC, which could represent a compensatory mechanism against oxidative stress. Glutathione (GSH) levels were lower and similar between N and DC. The MDA levels were significantly decreased in liver of rats from DT250 and DT500, reaching levels similar those of N, suggesting that ClEtOH prevented lipoperoxidation. The treatment of diabetic rats with ClEtOH also increased the GSH levels, as well as increased the GSH-Px activity, and did not change the SOD activity. The results of in vitro radical scavenging capacity indicated that ClEtOH is highly active. Conclusion: These findings indicate that ClEtOH has antioxidant properties in liver of diabetic rats, decreasing lipoperoxidation and increasing the endogenous antioxidant responses. Both the antihyperglycemic effect and the capacity to scavenge free radicals may be related to the antioxidant activity of ClEtOH in diabetes.
publishDate 2014
dc.date.none.fl_str_mv 2014
2016-01-28T16:56:15Z
2016-01-28T16:56:15Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.9734/bjpr/2014/13558
British Journal of Pharmaceutical Research, v. 4, p. 2340-2356, 2014.
2231-2919
http://hdl.handle.net/11449/133702
10.9734/bjpr/2014/13558
ISSN2231-2919-2014-04-2340-2356.pdf
3736475025187750
1624929129014716
url http://dx.doi.org/10.9734/bjpr/2014/13558
http://hdl.handle.net/11449/133702
identifier_str_mv British Journal of Pharmaceutical Research, v. 4, p. 2340-2356, 2014.
2231-2919
10.9734/bjpr/2014/13558
ISSN2231-2919-2014-04-2340-2356.pdf
3736475025187750
1624929129014716
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv British Journal of Pharmaceutical Research
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 2340-2356
application/pdf
dc.source.none.fl_str_mv Currículo Lattes
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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