Biofilm production under different atmospheres and growth media by Streptococcus agalactiae isolated from milk of cows with subclinical mastitis

Detalhes bibliográficos
Autor(a) principal: Bonsaglia, Erika C. R. [UNESP]
Data de Publicação: 2019
Outros Autores: Latosinski, Giulia S. [UNESP], Rossi, Rodolfo S. [UNESP], Rossi, Bruna F. [UNESP], Possebon, Fábio S. [UNESP], Pantoja, José Carlos F. [UNESP], Fernandes Júnior, Ary [UNESP], Rall, Vera L. M. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1007/s00203-019-01727-8
http://hdl.handle.net/11449/189604
Resumo: Different methods to analyze Streptococcus agalactiae biofilm formation have been investigated, but standardized protocols have not been developed. We compared S. agalactiae biofilm production among different atmospheres and growth media. Biofilm formation was studied in 32 isolates from bovine mastitis cases grown in Tryptone Soy Broth (TSB), Todd Hewitt Broth (THB), Luria Bertani Broth (LB) and Brain Heart Infusion (BHI), under two atmospheres, aerobic and 5% CO2. Regardless of the culture medium, growth under 5% CO2 resulted in a greater proportion of biofilm formation (65.63%), as compared with aerobic conditions (39.84%). Regardless of the atmosphere, the chances of biofilm formation were greater for isolates grown in TSB, as compared with THB [Odds ratio (OR) = 3.02], BHI (OR = 4.57), or LB (OR = 10.20). Thus, we suggest the use of 5% CO2 atmosphere and TSB in biofilm formation assays by Group-B streptococci (GBS) isolated from intramammary infections.
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spelling Biofilm production under different atmospheres and growth media by Streptococcus agalactiae isolated from milk of cows with subclinical mastitisBiofilmBovine mastitisMilkStreptococcus agalactiaeTryptone soy brothDifferent methods to analyze Streptococcus agalactiae biofilm formation have been investigated, but standardized protocols have not been developed. We compared S. agalactiae biofilm production among different atmospheres and growth media. Biofilm formation was studied in 32 isolates from bovine mastitis cases grown in Tryptone Soy Broth (TSB), Todd Hewitt Broth (THB), Luria Bertani Broth (LB) and Brain Heart Infusion (BHI), under two atmospheres, aerobic and 5% CO2. Regardless of the culture medium, growth under 5% CO2 resulted in a greater proportion of biofilm formation (65.63%), as compared with aerobic conditions (39.84%). Regardless of the atmosphere, the chances of biofilm formation were greater for isolates grown in TSB, as compared with THB [Odds ratio (OR) = 3.02], BHI (OR = 4.57), or LB (OR = 10.20). Thus, we suggest the use of 5% CO2 atmosphere and TSB in biofilm formation assays by Group-B streptococci (GBS) isolated from intramammary infections.Department of Microbiology and Immunology Institute of Biosciences Sao Paulo State University (UNESP), Postal Office Box 510Department of Veterinary Hygiene and Public Health Sao Paulo State University (UNESP)Department of Microbiology and Immunology Institute of Biosciences Sao Paulo State University (UNESP), Postal Office Box 510Department of Veterinary Hygiene and Public Health Sao Paulo State University (UNESP)Universidade Estadual Paulista (Unesp)Bonsaglia, Erika C. R. [UNESP]Latosinski, Giulia S. [UNESP]Rossi, Rodolfo S. [UNESP]Rossi, Bruna F. [UNESP]Possebon, Fábio S. [UNESP]Pantoja, José Carlos F. [UNESP]Fernandes Júnior, Ary [UNESP]Rall, Vera L. M. [UNESP]2019-10-06T16:45:58Z2019-10-06T16:45:58Z2019-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1007/s00203-019-01727-8Archives of Microbiology.1432-072X0302-8933http://hdl.handle.net/11449/18960410.1007/s00203-019-01727-82-s2.0-85071758125Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengArchives of Microbiologyinfo:eu-repo/semantics/openAccess2021-10-23T00:57:14Zoai:repositorio.unesp.br:11449/189604Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:49:13.919354Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Biofilm production under different atmospheres and growth media by Streptococcus agalactiae isolated from milk of cows with subclinical mastitis
title Biofilm production under different atmospheres and growth media by Streptococcus agalactiae isolated from milk of cows with subclinical mastitis
spellingShingle Biofilm production under different atmospheres and growth media by Streptococcus agalactiae isolated from milk of cows with subclinical mastitis
Bonsaglia, Erika C. R. [UNESP]
Biofilm
Bovine mastitis
Milk
Streptococcus agalactiae
Tryptone soy broth
title_short Biofilm production under different atmospheres and growth media by Streptococcus agalactiae isolated from milk of cows with subclinical mastitis
title_full Biofilm production under different atmospheres and growth media by Streptococcus agalactiae isolated from milk of cows with subclinical mastitis
title_fullStr Biofilm production under different atmospheres and growth media by Streptococcus agalactiae isolated from milk of cows with subclinical mastitis
title_full_unstemmed Biofilm production under different atmospheres and growth media by Streptococcus agalactiae isolated from milk of cows with subclinical mastitis
title_sort Biofilm production under different atmospheres and growth media by Streptococcus agalactiae isolated from milk of cows with subclinical mastitis
author Bonsaglia, Erika C. R. [UNESP]
author_facet Bonsaglia, Erika C. R. [UNESP]
Latosinski, Giulia S. [UNESP]
Rossi, Rodolfo S. [UNESP]
Rossi, Bruna F. [UNESP]
Possebon, Fábio S. [UNESP]
Pantoja, José Carlos F. [UNESP]
Fernandes Júnior, Ary [UNESP]
Rall, Vera L. M. [UNESP]
author_role author
author2 Latosinski, Giulia S. [UNESP]
Rossi, Rodolfo S. [UNESP]
Rossi, Bruna F. [UNESP]
Possebon, Fábio S. [UNESP]
Pantoja, José Carlos F. [UNESP]
Fernandes Júnior, Ary [UNESP]
Rall, Vera L. M. [UNESP]
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Bonsaglia, Erika C. R. [UNESP]
Latosinski, Giulia S. [UNESP]
Rossi, Rodolfo S. [UNESP]
Rossi, Bruna F. [UNESP]
Possebon, Fábio S. [UNESP]
Pantoja, José Carlos F. [UNESP]
Fernandes Júnior, Ary [UNESP]
Rall, Vera L. M. [UNESP]
dc.subject.por.fl_str_mv Biofilm
Bovine mastitis
Milk
Streptococcus agalactiae
Tryptone soy broth
topic Biofilm
Bovine mastitis
Milk
Streptococcus agalactiae
Tryptone soy broth
description Different methods to analyze Streptococcus agalactiae biofilm formation have been investigated, but standardized protocols have not been developed. We compared S. agalactiae biofilm production among different atmospheres and growth media. Biofilm formation was studied in 32 isolates from bovine mastitis cases grown in Tryptone Soy Broth (TSB), Todd Hewitt Broth (THB), Luria Bertani Broth (LB) and Brain Heart Infusion (BHI), under two atmospheres, aerobic and 5% CO2. Regardless of the culture medium, growth under 5% CO2 resulted in a greater proportion of biofilm formation (65.63%), as compared with aerobic conditions (39.84%). Regardless of the atmosphere, the chances of biofilm formation were greater for isolates grown in TSB, as compared with THB [Odds ratio (OR) = 3.02], BHI (OR = 4.57), or LB (OR = 10.20). Thus, we suggest the use of 5% CO2 atmosphere and TSB in biofilm formation assays by Group-B streptococci (GBS) isolated from intramammary infections.
publishDate 2019
dc.date.none.fl_str_mv 2019-10-06T16:45:58Z
2019-10-06T16:45:58Z
2019-01-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1007/s00203-019-01727-8
Archives of Microbiology.
1432-072X
0302-8933
http://hdl.handle.net/11449/189604
10.1007/s00203-019-01727-8
2-s2.0-85071758125
url http://dx.doi.org/10.1007/s00203-019-01727-8
http://hdl.handle.net/11449/189604
identifier_str_mv Archives of Microbiology.
1432-072X
0302-8933
10.1007/s00203-019-01727-8
2-s2.0-85071758125
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Archives of Microbiology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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