Biofilm production under different atmospheres and growth media by Streptococcus agalactiae isolated from milk of cows with subclinical mastitis
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s00203-019-01727-8 http://hdl.handle.net/11449/189604 |
Resumo: | Different methods to analyze Streptococcus agalactiae biofilm formation have been investigated, but standardized protocols have not been developed. We compared S. agalactiae biofilm production among different atmospheres and growth media. Biofilm formation was studied in 32 isolates from bovine mastitis cases grown in Tryptone Soy Broth (TSB), Todd Hewitt Broth (THB), Luria Bertani Broth (LB) and Brain Heart Infusion (BHI), under two atmospheres, aerobic and 5% CO2. Regardless of the culture medium, growth under 5% CO2 resulted in a greater proportion of biofilm formation (65.63%), as compared with aerobic conditions (39.84%). Regardless of the atmosphere, the chances of biofilm formation were greater for isolates grown in TSB, as compared with THB [Odds ratio (OR) = 3.02], BHI (OR = 4.57), or LB (OR = 10.20). Thus, we suggest the use of 5% CO2 atmosphere and TSB in biofilm formation assays by Group-B streptococci (GBS) isolated from intramammary infections. |
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Biofilm production under different atmospheres and growth media by Streptococcus agalactiae isolated from milk of cows with subclinical mastitisBiofilmBovine mastitisMilkStreptococcus agalactiaeTryptone soy brothDifferent methods to analyze Streptococcus agalactiae biofilm formation have been investigated, but standardized protocols have not been developed. We compared S. agalactiae biofilm production among different atmospheres and growth media. Biofilm formation was studied in 32 isolates from bovine mastitis cases grown in Tryptone Soy Broth (TSB), Todd Hewitt Broth (THB), Luria Bertani Broth (LB) and Brain Heart Infusion (BHI), under two atmospheres, aerobic and 5% CO2. Regardless of the culture medium, growth under 5% CO2 resulted in a greater proportion of biofilm formation (65.63%), as compared with aerobic conditions (39.84%). Regardless of the atmosphere, the chances of biofilm formation were greater for isolates grown in TSB, as compared with THB [Odds ratio (OR) = 3.02], BHI (OR = 4.57), or LB (OR = 10.20). Thus, we suggest the use of 5% CO2 atmosphere and TSB in biofilm formation assays by Group-B streptococci (GBS) isolated from intramammary infections.Department of Microbiology and Immunology Institute of Biosciences Sao Paulo State University (UNESP), Postal Office Box 510Department of Veterinary Hygiene and Public Health Sao Paulo State University (UNESP)Department of Microbiology and Immunology Institute of Biosciences Sao Paulo State University (UNESP), Postal Office Box 510Department of Veterinary Hygiene and Public Health Sao Paulo State University (UNESP)Universidade Estadual Paulista (Unesp)Bonsaglia, Erika C. R. [UNESP]Latosinski, Giulia S. [UNESP]Rossi, Rodolfo S. [UNESP]Rossi, Bruna F. [UNESP]Possebon, Fábio S. [UNESP]Pantoja, José Carlos F. [UNESP]Fernandes Júnior, Ary [UNESP]Rall, Vera L. M. [UNESP]2019-10-06T16:45:58Z2019-10-06T16:45:58Z2019-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1007/s00203-019-01727-8Archives of Microbiology.1432-072X0302-8933http://hdl.handle.net/11449/18960410.1007/s00203-019-01727-82-s2.0-85071758125Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengArchives of Microbiologyinfo:eu-repo/semantics/openAccess2021-10-23T00:57:14Zoai:repositorio.unesp.br:11449/189604Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:49:13.919354Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Biofilm production under different atmospheres and growth media by Streptococcus agalactiae isolated from milk of cows with subclinical mastitis |
title |
Biofilm production under different atmospheres and growth media by Streptococcus agalactiae isolated from milk of cows with subclinical mastitis |
spellingShingle |
Biofilm production under different atmospheres and growth media by Streptococcus agalactiae isolated from milk of cows with subclinical mastitis Bonsaglia, Erika C. R. [UNESP] Biofilm Bovine mastitis Milk Streptococcus agalactiae Tryptone soy broth |
title_short |
Biofilm production under different atmospheres and growth media by Streptococcus agalactiae isolated from milk of cows with subclinical mastitis |
title_full |
Biofilm production under different atmospheres and growth media by Streptococcus agalactiae isolated from milk of cows with subclinical mastitis |
title_fullStr |
Biofilm production under different atmospheres and growth media by Streptococcus agalactiae isolated from milk of cows with subclinical mastitis |
title_full_unstemmed |
Biofilm production under different atmospheres and growth media by Streptococcus agalactiae isolated from milk of cows with subclinical mastitis |
title_sort |
Biofilm production under different atmospheres and growth media by Streptococcus agalactiae isolated from milk of cows with subclinical mastitis |
author |
Bonsaglia, Erika C. R. [UNESP] |
author_facet |
Bonsaglia, Erika C. R. [UNESP] Latosinski, Giulia S. [UNESP] Rossi, Rodolfo S. [UNESP] Rossi, Bruna F. [UNESP] Possebon, Fábio S. [UNESP] Pantoja, José Carlos F. [UNESP] Fernandes Júnior, Ary [UNESP] Rall, Vera L. M. [UNESP] |
author_role |
author |
author2 |
Latosinski, Giulia S. [UNESP] Rossi, Rodolfo S. [UNESP] Rossi, Bruna F. [UNESP] Possebon, Fábio S. [UNESP] Pantoja, José Carlos F. [UNESP] Fernandes Júnior, Ary [UNESP] Rall, Vera L. M. [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Bonsaglia, Erika C. R. [UNESP] Latosinski, Giulia S. [UNESP] Rossi, Rodolfo S. [UNESP] Rossi, Bruna F. [UNESP] Possebon, Fábio S. [UNESP] Pantoja, José Carlos F. [UNESP] Fernandes Júnior, Ary [UNESP] Rall, Vera L. M. [UNESP] |
dc.subject.por.fl_str_mv |
Biofilm Bovine mastitis Milk Streptococcus agalactiae Tryptone soy broth |
topic |
Biofilm Bovine mastitis Milk Streptococcus agalactiae Tryptone soy broth |
description |
Different methods to analyze Streptococcus agalactiae biofilm formation have been investigated, but standardized protocols have not been developed. We compared S. agalactiae biofilm production among different atmospheres and growth media. Biofilm formation was studied in 32 isolates from bovine mastitis cases grown in Tryptone Soy Broth (TSB), Todd Hewitt Broth (THB), Luria Bertani Broth (LB) and Brain Heart Infusion (BHI), under two atmospheres, aerobic and 5% CO2. Regardless of the culture medium, growth under 5% CO2 resulted in a greater proportion of biofilm formation (65.63%), as compared with aerobic conditions (39.84%). Regardless of the atmosphere, the chances of biofilm formation were greater for isolates grown in TSB, as compared with THB [Odds ratio (OR) = 3.02], BHI (OR = 4.57), or LB (OR = 10.20). Thus, we suggest the use of 5% CO2 atmosphere and TSB in biofilm formation assays by Group-B streptococci (GBS) isolated from intramammary infections. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-06T16:45:58Z 2019-10-06T16:45:58Z 2019-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s00203-019-01727-8 Archives of Microbiology. 1432-072X 0302-8933 http://hdl.handle.net/11449/189604 10.1007/s00203-019-01727-8 2-s2.0-85071758125 |
url |
http://dx.doi.org/10.1007/s00203-019-01727-8 http://hdl.handle.net/11449/189604 |
identifier_str_mv |
Archives of Microbiology. 1432-072X 0302-8933 10.1007/s00203-019-01727-8 2-s2.0-85071758125 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Archives of Microbiology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129361531895808 |