Detecção sorológica e molecular de Anaplasma marginale em búfalos na Ilha de Marajó, Pará

• Autor(a) principal: Silva, Jenevaldo Barbosa Da [UNESP]
• Data de Publicação: 2014
• Outros Autores: Lopes, Cinthia Távora De Albuquerque, Souza, Melina Garcia Saraiva De, Gibson, André Felipe Bagarrão, Vinhote, Wagner Marcelo De Souza, Fonseca, Adivaldo Henrique Da, Araújo, Flábio Ribeiro De, Barbosa-neto, José Diomedes
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.1590/S0100-736X2014000100002; http://hdl.handle.net/11449/110046
• topic: Anaplasma marginale; anaplasmose; bubalinos; ELISA; MSP5; PCR; Anaplasma marginale; anaplasmosis; Buffaloes; ELISA; MSP5; PCR

The aim of the study was to test the molecular and serological prevalence of Anaplasma marginale in water buffaloes of the Marajó Island, State of Pará, Brazil. For serologic research were randomly selected 800 buffaloes and for molecular research 50 of these animals were randomly chosen. To quantify the serological prevalence we used the indirect enzyme linked immunosorbent assay (iELISA) with total antigen containing proteins outer surface. To quantify the prevalence molecular was used the polymerase chain reaction (PCR) involving gene amplification fragment larger surface protein 5 (MSP5). The prevalence of positive animals in iELISA was 25% (200/800). In the PCR we detected the presence of A. marginale in 2% (1/50) of animals. Although only one animal was positive in PCR, we found that it was negative in ELISA. The presence of the agent, even in low prevalence, shows that buffaloes can act as an important reservoir for transmission of the pathogen to cattle in northern Brazil.