Antifungal activity and biocompatibility of α-AgVO3 microcrystals: A promising material against oral Candida disease
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.msec.2019.110405 http://hdl.handle.net/11449/201310 |
Resumo: | The number of studies on microcrystals containing silver has increased in recent decades. Among the silver-containing microcrystals, α-AgVO3 has gained prominence owing to its polymorphism that allows it to exert interesting antimicrobial activity against pathogenic microorganisms. The aim of this study was to evaluate the antifungal activity and cytotoxicity of three different α-AgVO3 microcrystals when in solution. α-AgVO3 microcrystals were synthesized using the co-precipitation method at three different temperatures (10 °C, 20 °C, and 30 °C), and then characterized by X-ray diffraction and scanning electron microscopy. The antifungal activity of α-AgVO3 microcrystals against Candida albicans was determined by estimating the minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC). Fluorescence images were obtained to confirm antifungal concentrations. To assess the biocompatibility of microcrystals applied at MIC and MFC on keratinocytes cells (NOK-si), an Alamar Blue assay, scanning electron microscopy, and a DNA gel integrity test were carried out. The quantitative and qualitative results showed that, regardless of the co-precipitation method used to synthetize α-AgVO3 microcrystals, C. albicans growth was visibly inhibited at 3.9 μg/mL (MIC) and completely inhibited at 15.62 μg/mL (MFC). The cytotoxic and genotoxic outcomes revealed that the MIC and MFC concentrations did not affect NOK-si cell morphology, proliferation, or DNA integrity. The search for new antimicrobial materials has been the focus of the research community recently because of increases in microbial resistance. The findings reported herein demonstrate a novel antifungal and non-cytotoxic material that could be used in biomedical and dental applications. |
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Antifungal activity and biocompatibility of α-AgVO3 microcrystals: A promising material against oral Candida diseaseAntifungal agentsBiocompatibilityCandida albicansKeratinocytesThe number of studies on microcrystals containing silver has increased in recent decades. Among the silver-containing microcrystals, α-AgVO3 has gained prominence owing to its polymorphism that allows it to exert interesting antimicrobial activity against pathogenic microorganisms. The aim of this study was to evaluate the antifungal activity and cytotoxicity of three different α-AgVO3 microcrystals when in solution. α-AgVO3 microcrystals were synthesized using the co-precipitation method at three different temperatures (10 °C, 20 °C, and 30 °C), and then characterized by X-ray diffraction and scanning electron microscopy. The antifungal activity of α-AgVO3 microcrystals against Candida albicans was determined by estimating the minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC). Fluorescence images were obtained to confirm antifungal concentrations. To assess the biocompatibility of microcrystals applied at MIC and MFC on keratinocytes cells (NOK-si), an Alamar Blue assay, scanning electron microscopy, and a DNA gel integrity test were carried out. The quantitative and qualitative results showed that, regardless of the co-precipitation method used to synthetize α-AgVO3 microcrystals, C. albicans growth was visibly inhibited at 3.9 μg/mL (MIC) and completely inhibited at 15.62 μg/mL (MFC). The cytotoxic and genotoxic outcomes revealed that the MIC and MFC concentrations did not affect NOK-si cell morphology, proliferation, or DNA integrity. The search for new antimicrobial materials has been the focus of the research community recently because of increases in microbial resistance. The findings reported herein demonstrate a novel antifungal and non-cytotoxic material that could be used in biomedical and dental applications.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)São Paulo State University (UNESP) School of Dentistry Araraquara, Rua Humaita, 1680CDMF-UFSCar - Universidade Federal de São Carlos, Washington Luis km 235, P.O. Box 676São Paulo State University (UNESP) School of Dentistry Araraquara, Rua Humaita, 1680FAPESP: 2013/07296-2FAPESP: 2015/03567-7FAPESP: 2015/03654-7FAPESP: 2015/13834-2FAPESP: 2015/25124-0Universidade Estadual Paulista (Unesp)Universidade Federal de São Carlos (UFSCar)Pimentel, Bruna Natália Alves da Silva [UNESP]de Foggi, Camila Cristina [UNESP]Barbugli, Paula Aboud [UNESP]de Oliveira, Regiane Cristinade Avila, Erica Dorigatti [UNESP]Longo, ElsonVergani, Carlos Eduardo [UNESP]2020-12-12T02:29:22Z2020-12-12T02:29:22Z2020-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.msec.2019.110405Materials Science and Engineering C, v. 108.1873-01910928-4931http://hdl.handle.net/11449/20131010.1016/j.msec.2019.1104052-s2.0-85074684916Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMaterials Science and Engineering Cinfo:eu-repo/semantics/openAccess2024-09-27T14:56:23Zoai:repositorio.unesp.br:11449/201310Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-27T14:56:23Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Antifungal activity and biocompatibility of α-AgVO3 microcrystals: A promising material against oral Candida disease |
title |
Antifungal activity and biocompatibility of α-AgVO3 microcrystals: A promising material against oral Candida disease |
spellingShingle |
Antifungal activity and biocompatibility of α-AgVO3 microcrystals: A promising material against oral Candida disease Pimentel, Bruna Natália Alves da Silva [UNESP] Antifungal agents Biocompatibility Candida albicans Keratinocytes |
title_short |
Antifungal activity and biocompatibility of α-AgVO3 microcrystals: A promising material against oral Candida disease |
title_full |
Antifungal activity and biocompatibility of α-AgVO3 microcrystals: A promising material against oral Candida disease |
title_fullStr |
Antifungal activity and biocompatibility of α-AgVO3 microcrystals: A promising material against oral Candida disease |
title_full_unstemmed |
Antifungal activity and biocompatibility of α-AgVO3 microcrystals: A promising material against oral Candida disease |
title_sort |
Antifungal activity and biocompatibility of α-AgVO3 microcrystals: A promising material against oral Candida disease |
author |
Pimentel, Bruna Natália Alves da Silva [UNESP] |
author_facet |
Pimentel, Bruna Natália Alves da Silva [UNESP] de Foggi, Camila Cristina [UNESP] Barbugli, Paula Aboud [UNESP] de Oliveira, Regiane Cristina de Avila, Erica Dorigatti [UNESP] Longo, Elson Vergani, Carlos Eduardo [UNESP] |
author_role |
author |
author2 |
de Foggi, Camila Cristina [UNESP] Barbugli, Paula Aboud [UNESP] de Oliveira, Regiane Cristina de Avila, Erica Dorigatti [UNESP] Longo, Elson Vergani, Carlos Eduardo [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Federal de São Carlos (UFSCar) |
dc.contributor.author.fl_str_mv |
Pimentel, Bruna Natália Alves da Silva [UNESP] de Foggi, Camila Cristina [UNESP] Barbugli, Paula Aboud [UNESP] de Oliveira, Regiane Cristina de Avila, Erica Dorigatti [UNESP] Longo, Elson Vergani, Carlos Eduardo [UNESP] |
dc.subject.por.fl_str_mv |
Antifungal agents Biocompatibility Candida albicans Keratinocytes |
topic |
Antifungal agents Biocompatibility Candida albicans Keratinocytes |
description |
The number of studies on microcrystals containing silver has increased in recent decades. Among the silver-containing microcrystals, α-AgVO3 has gained prominence owing to its polymorphism that allows it to exert interesting antimicrobial activity against pathogenic microorganisms. The aim of this study was to evaluate the antifungal activity and cytotoxicity of three different α-AgVO3 microcrystals when in solution. α-AgVO3 microcrystals were synthesized using the co-precipitation method at three different temperatures (10 °C, 20 °C, and 30 °C), and then characterized by X-ray diffraction and scanning electron microscopy. The antifungal activity of α-AgVO3 microcrystals against Candida albicans was determined by estimating the minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC). Fluorescence images were obtained to confirm antifungal concentrations. To assess the biocompatibility of microcrystals applied at MIC and MFC on keratinocytes cells (NOK-si), an Alamar Blue assay, scanning electron microscopy, and a DNA gel integrity test were carried out. The quantitative and qualitative results showed that, regardless of the co-precipitation method used to synthetize α-AgVO3 microcrystals, C. albicans growth was visibly inhibited at 3.9 μg/mL (MIC) and completely inhibited at 15.62 μg/mL (MFC). The cytotoxic and genotoxic outcomes revealed that the MIC and MFC concentrations did not affect NOK-si cell morphology, proliferation, or DNA integrity. The search for new antimicrobial materials has been the focus of the research community recently because of increases in microbial resistance. The findings reported herein demonstrate a novel antifungal and non-cytotoxic material that could be used in biomedical and dental applications. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T02:29:22Z 2020-12-12T02:29:22Z 2020-03-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.msec.2019.110405 Materials Science and Engineering C, v. 108. 1873-0191 0928-4931 http://hdl.handle.net/11449/201310 10.1016/j.msec.2019.110405 2-s2.0-85074684916 |
url |
http://dx.doi.org/10.1016/j.msec.2019.110405 http://hdl.handle.net/11449/201310 |
identifier_str_mv |
Materials Science and Engineering C, v. 108. 1873-0191 0928-4931 10.1016/j.msec.2019.110405 2-s2.0-85074684916 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Materials Science and Engineering C |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
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1813546385154244608 |