Antifungal activity and biocompatibility of α-AgVO3 microcrystals: A promising material against oral Candida disease

Detalhes bibliográficos
Autor(a) principal: Pimentel, Bruna Natália Alves da Silva [UNESP]
Data de Publicação: 2020
Outros Autores: de Foggi, Camila Cristina [UNESP], Barbugli, Paula Aboud [UNESP], de Oliveira, Regiane Cristina, de Avila, Erica Dorigatti [UNESP], Longo, Elson, Vergani, Carlos Eduardo [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.msec.2019.110405
http://hdl.handle.net/11449/201310
Resumo: The number of studies on microcrystals containing silver has increased in recent decades. Among the silver-containing microcrystals, α-AgVO3 has gained prominence owing to its polymorphism that allows it to exert interesting antimicrobial activity against pathogenic microorganisms. The aim of this study was to evaluate the antifungal activity and cytotoxicity of three different α-AgVO3 microcrystals when in solution. α-AgVO3 microcrystals were synthesized using the co-precipitation method at three different temperatures (10 °C, 20 °C, and 30 °C), and then characterized by X-ray diffraction and scanning electron microscopy. The antifungal activity of α-AgVO3 microcrystals against Candida albicans was determined by estimating the minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC). Fluorescence images were obtained to confirm antifungal concentrations. To assess the biocompatibility of microcrystals applied at MIC and MFC on keratinocytes cells (NOK-si), an Alamar Blue assay, scanning electron microscopy, and a DNA gel integrity test were carried out. The quantitative and qualitative results showed that, regardless of the co-precipitation method used to synthetize α-AgVO3 microcrystals, C. albicans growth was visibly inhibited at 3.9 μg/mL (MIC) and completely inhibited at 15.62 μg/mL (MFC). The cytotoxic and genotoxic outcomes revealed that the MIC and MFC concentrations did not affect NOK-si cell morphology, proliferation, or DNA integrity. The search for new antimicrobial materials has been the focus of the research community recently because of increases in microbial resistance. The findings reported herein demonstrate a novel antifungal and non-cytotoxic material that could be used in biomedical and dental applications.
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spelling Antifungal activity and biocompatibility of α-AgVO3 microcrystals: A promising material against oral Candida diseaseAntifungal agentsBiocompatibilityCandida albicansKeratinocytesThe number of studies on microcrystals containing silver has increased in recent decades. Among the silver-containing microcrystals, α-AgVO3 has gained prominence owing to its polymorphism that allows it to exert interesting antimicrobial activity against pathogenic microorganisms. The aim of this study was to evaluate the antifungal activity and cytotoxicity of three different α-AgVO3 microcrystals when in solution. α-AgVO3 microcrystals were synthesized using the co-precipitation method at three different temperatures (10 °C, 20 °C, and 30 °C), and then characterized by X-ray diffraction and scanning electron microscopy. The antifungal activity of α-AgVO3 microcrystals against Candida albicans was determined by estimating the minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC). Fluorescence images were obtained to confirm antifungal concentrations. To assess the biocompatibility of microcrystals applied at MIC and MFC on keratinocytes cells (NOK-si), an Alamar Blue assay, scanning electron microscopy, and a DNA gel integrity test were carried out. The quantitative and qualitative results showed that, regardless of the co-precipitation method used to synthetize α-AgVO3 microcrystals, C. albicans growth was visibly inhibited at 3.9 μg/mL (MIC) and completely inhibited at 15.62 μg/mL (MFC). The cytotoxic and genotoxic outcomes revealed that the MIC and MFC concentrations did not affect NOK-si cell morphology, proliferation, or DNA integrity. The search for new antimicrobial materials has been the focus of the research community recently because of increases in microbial resistance. The findings reported herein demonstrate a novel antifungal and non-cytotoxic material that could be used in biomedical and dental applications.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)São Paulo State University (UNESP) School of Dentistry Araraquara, Rua Humaita, 1680CDMF-UFSCar - Universidade Federal de São Carlos, Washington Luis km 235, P.O. Box 676São Paulo State University (UNESP) School of Dentistry Araraquara, Rua Humaita, 1680FAPESP: 2013/07296-2FAPESP: 2015/03567-7FAPESP: 2015/03654-7FAPESP: 2015/13834-2FAPESP: 2015/25124-0Universidade Estadual Paulista (Unesp)Universidade Federal de São Carlos (UFSCar)Pimentel, Bruna Natália Alves da Silva [UNESP]de Foggi, Camila Cristina [UNESP]Barbugli, Paula Aboud [UNESP]de Oliveira, Regiane Cristinade Avila, Erica Dorigatti [UNESP]Longo, ElsonVergani, Carlos Eduardo [UNESP]2020-12-12T02:29:22Z2020-12-12T02:29:22Z2020-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.msec.2019.110405Materials Science and Engineering C, v. 108.1873-01910928-4931http://hdl.handle.net/11449/20131010.1016/j.msec.2019.1104052-s2.0-85074684916Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMaterials Science and Engineering Cinfo:eu-repo/semantics/openAccess2024-09-27T14:56:23Zoai:repositorio.unesp.br:11449/201310Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-27T14:56:23Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Antifungal activity and biocompatibility of α-AgVO3 microcrystals: A promising material against oral Candida disease
title Antifungal activity and biocompatibility of α-AgVO3 microcrystals: A promising material against oral Candida disease
spellingShingle Antifungal activity and biocompatibility of α-AgVO3 microcrystals: A promising material against oral Candida disease
Pimentel, Bruna Natália Alves da Silva [UNESP]
Antifungal agents
Biocompatibility
Candida albicans
Keratinocytes
title_short Antifungal activity and biocompatibility of α-AgVO3 microcrystals: A promising material against oral Candida disease
title_full Antifungal activity and biocompatibility of α-AgVO3 microcrystals: A promising material against oral Candida disease
title_fullStr Antifungal activity and biocompatibility of α-AgVO3 microcrystals: A promising material against oral Candida disease
title_full_unstemmed Antifungal activity and biocompatibility of α-AgVO3 microcrystals: A promising material against oral Candida disease
title_sort Antifungal activity and biocompatibility of α-AgVO3 microcrystals: A promising material against oral Candida disease
author Pimentel, Bruna Natália Alves da Silva [UNESP]
author_facet Pimentel, Bruna Natália Alves da Silva [UNESP]
de Foggi, Camila Cristina [UNESP]
Barbugli, Paula Aboud [UNESP]
de Oliveira, Regiane Cristina
de Avila, Erica Dorigatti [UNESP]
Longo, Elson
Vergani, Carlos Eduardo [UNESP]
author_role author
author2 de Foggi, Camila Cristina [UNESP]
Barbugli, Paula Aboud [UNESP]
de Oliveira, Regiane Cristina
de Avila, Erica Dorigatti [UNESP]
Longo, Elson
Vergani, Carlos Eduardo [UNESP]
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidade Federal de São Carlos (UFSCar)
dc.contributor.author.fl_str_mv Pimentel, Bruna Natália Alves da Silva [UNESP]
de Foggi, Camila Cristina [UNESP]
Barbugli, Paula Aboud [UNESP]
de Oliveira, Regiane Cristina
de Avila, Erica Dorigatti [UNESP]
Longo, Elson
Vergani, Carlos Eduardo [UNESP]
dc.subject.por.fl_str_mv Antifungal agents
Biocompatibility
Candida albicans
Keratinocytes
topic Antifungal agents
Biocompatibility
Candida albicans
Keratinocytes
description The number of studies on microcrystals containing silver has increased in recent decades. Among the silver-containing microcrystals, α-AgVO3 has gained prominence owing to its polymorphism that allows it to exert interesting antimicrobial activity against pathogenic microorganisms. The aim of this study was to evaluate the antifungal activity and cytotoxicity of three different α-AgVO3 microcrystals when in solution. α-AgVO3 microcrystals were synthesized using the co-precipitation method at three different temperatures (10 °C, 20 °C, and 30 °C), and then characterized by X-ray diffraction and scanning electron microscopy. The antifungal activity of α-AgVO3 microcrystals against Candida albicans was determined by estimating the minimum inhibitory concentration (MIC) and minimum fungicidal concentration (MFC). Fluorescence images were obtained to confirm antifungal concentrations. To assess the biocompatibility of microcrystals applied at MIC and MFC on keratinocytes cells (NOK-si), an Alamar Blue assay, scanning electron microscopy, and a DNA gel integrity test were carried out. The quantitative and qualitative results showed that, regardless of the co-precipitation method used to synthetize α-AgVO3 microcrystals, C. albicans growth was visibly inhibited at 3.9 μg/mL (MIC) and completely inhibited at 15.62 μg/mL (MFC). The cytotoxic and genotoxic outcomes revealed that the MIC and MFC concentrations did not affect NOK-si cell morphology, proliferation, or DNA integrity. The search for new antimicrobial materials has been the focus of the research community recently because of increases in microbial resistance. The findings reported herein demonstrate a novel antifungal and non-cytotoxic material that could be used in biomedical and dental applications.
publishDate 2020
dc.date.none.fl_str_mv 2020-12-12T02:29:22Z
2020-12-12T02:29:22Z
2020-03-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.msec.2019.110405
Materials Science and Engineering C, v. 108.
1873-0191
0928-4931
http://hdl.handle.net/11449/201310
10.1016/j.msec.2019.110405
2-s2.0-85074684916
url http://dx.doi.org/10.1016/j.msec.2019.110405
http://hdl.handle.net/11449/201310
identifier_str_mv Materials Science and Engineering C, v. 108.
1873-0191
0928-4931
10.1016/j.msec.2019.110405
2-s2.0-85074684916
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Materials Science and Engineering C
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
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