Synergism and Antagonism between Bacillus thuringiensis Vip3A and Cry1 Proteins in Heliothis virescens, Diatraea saccharalis and Spodoptera frugiperda
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
DOI: | 10.1371/journal.pone.0107196 |
Texto Completo: | http://dx.doi.org/10.1371/journal.pone.0107196 http://hdl.handle.net/11449/117408 |
Resumo: | Second generation Bt crops (insect resistant crops carrying Bacillus thuringiensis genes) combine more than one gene that codes for insecticidal proteins in the same plant to provide better control of agricultural pests. Some of the new combinations involve co-expression of cry and vip genes. Because Cry and Vip proteins have different midgut targets and possibly different mechanisms of toxicity, it is important to evaluate possible synergistic or antagonistic interactions between these two classes of toxins. Three members of the Cry1 class of proteins and three from the Vip3A class were tested against Heliothis virescens for possible interactions. At the level of LC50, Cry1Ac was the most active protein, whereas the rest of proteins tested were similarly active. However, at the level of LC90, Cry1Aa and Cry1Ca were the least active proteins, and Cry1Ac and Vip3A proteins were not significantly different. Under the experimental conditions used in this study, we found an antagonistic effect of Cry1Ca with the three Vip3A proteins. The interaction between Cry1Ca and Vip3Aa was also tested on two other species of Lepidoptera. Whereas antagonism was observed in Spodoptera frugiperda, synergism was found in Diatraea saccharalis. In all cases, the interaction between Vip3A and Cry1 proteins was more evident at the LC90 level than at the LC50 level. The fact that the same combination of proteins may result in a synergistic or an antagonistic interaction may be an indication that there are different types of interactions within the host, depending on the insect species tested. |
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Synergism and Antagonism between Bacillus thuringiensis Vip3A and Cry1 Proteins in Heliothis virescens, Diatraea saccharalis and Spodoptera frugiperdaSecond generation Bt crops (insect resistant crops carrying Bacillus thuringiensis genes) combine more than one gene that codes for insecticidal proteins in the same plant to provide better control of agricultural pests. Some of the new combinations involve co-expression of cry and vip genes. Because Cry and Vip proteins have different midgut targets and possibly different mechanisms of toxicity, it is important to evaluate possible synergistic or antagonistic interactions between these two classes of toxins. Three members of the Cry1 class of proteins and three from the Vip3A class were tested against Heliothis virescens for possible interactions. At the level of LC50, Cry1Ac was the most active protein, whereas the rest of proteins tested were similarly active. However, at the level of LC90, Cry1Aa and Cry1Ca were the least active proteins, and Cry1Ac and Vip3A proteins were not significantly different. Under the experimental conditions used in this study, we found an antagonistic effect of Cry1Ca with the three Vip3A proteins. The interaction between Cry1Ca and Vip3Aa was also tested on two other species of Lepidoptera. Whereas antagonism was observed in Spodoptera frugiperda, synergism was found in Diatraea saccharalis. In all cases, the interaction between Vip3A and Cry1 proteins was more evident at the LC90 level than at the LC50 level. The fact that the same combination of proteins may result in a synergistic or an antagonistic interaction may be an indication that there are different types of interactions within the host, depending on the insect species tested.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Spanish Ministry of Economy and CompetivityFEDERUniv Estadual Paulista, Dept Biol Aplicada Agr, UNESP, Fac Ciencias Agr & Vet, Sao Paulo, BrazilDept Fitossanidade, Jaboticabal, SP, BrazilUniv Valencia, Dept Genet, E-46100 Burjassot, Valencia, SpainUniv Estadual Paulista, Dept Biol Aplicada Agr, UNESP, Fac Ciencias Agr & Vet, Sao Paulo, BrazilCAPES: DGU-194/09Spanish Ministry of Economy and CompetivityAGL2012-39946-C02-01Public Library ScienceUniversidade Estadual Paulista (Unesp)Dept FitossanidadeUniv ValenciaNunes Lemes, Ana Rita [UNESP]Davolos, Camila Chiaradia [UNESP]Brunini Crialesi Legori, Paula Cristina [UNESP]Fernandes, Odair AparecidoFerre, JuanFranco Lemos, Manoel Victor [UNESP]Desiderio, Janete Apparecida [UNESP]2015-03-18T15:56:04Z2015-03-18T15:56:04Z2014-10-02info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article8application/pdfhttp://dx.doi.org/10.1371/journal.pone.0107196Plos One. San Francisco: Public Library Science, v. 9, n. 10, 8 p., 2014.1932-6203http://hdl.handle.net/11449/11740810.1371/journal.pone.0107196WOS:000342591500006WOS000342591500006.pdf1458288287757880717927306062476112950513405918360000-0003-3489-4754Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPlos One2.7661,164info:eu-repo/semantics/openAccess2024-06-06T13:05:09Zoai:repositorio.unesp.br:11449/117408Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:36:40.571417Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Synergism and Antagonism between Bacillus thuringiensis Vip3A and Cry1 Proteins in Heliothis virescens, Diatraea saccharalis and Spodoptera frugiperda |
title |
Synergism and Antagonism between Bacillus thuringiensis Vip3A and Cry1 Proteins in Heliothis virescens, Diatraea saccharalis and Spodoptera frugiperda |
spellingShingle |
Synergism and Antagonism between Bacillus thuringiensis Vip3A and Cry1 Proteins in Heliothis virescens, Diatraea saccharalis and Spodoptera frugiperda Synergism and Antagonism between Bacillus thuringiensis Vip3A and Cry1 Proteins in Heliothis virescens, Diatraea saccharalis and Spodoptera frugiperda Nunes Lemes, Ana Rita [UNESP] Nunes Lemes, Ana Rita [UNESP] |
title_short |
Synergism and Antagonism between Bacillus thuringiensis Vip3A and Cry1 Proteins in Heliothis virescens, Diatraea saccharalis and Spodoptera frugiperda |
title_full |
Synergism and Antagonism between Bacillus thuringiensis Vip3A and Cry1 Proteins in Heliothis virescens, Diatraea saccharalis and Spodoptera frugiperda |
title_fullStr |
Synergism and Antagonism between Bacillus thuringiensis Vip3A and Cry1 Proteins in Heliothis virescens, Diatraea saccharalis and Spodoptera frugiperda Synergism and Antagonism between Bacillus thuringiensis Vip3A and Cry1 Proteins in Heliothis virescens, Diatraea saccharalis and Spodoptera frugiperda |
title_full_unstemmed |
Synergism and Antagonism between Bacillus thuringiensis Vip3A and Cry1 Proteins in Heliothis virescens, Diatraea saccharalis and Spodoptera frugiperda Synergism and Antagonism between Bacillus thuringiensis Vip3A and Cry1 Proteins in Heliothis virescens, Diatraea saccharalis and Spodoptera frugiperda |
title_sort |
Synergism and Antagonism between Bacillus thuringiensis Vip3A and Cry1 Proteins in Heliothis virescens, Diatraea saccharalis and Spodoptera frugiperda |
author |
Nunes Lemes, Ana Rita [UNESP] |
author_facet |
Nunes Lemes, Ana Rita [UNESP] Nunes Lemes, Ana Rita [UNESP] Davolos, Camila Chiaradia [UNESP] Brunini Crialesi Legori, Paula Cristina [UNESP] Fernandes, Odair Aparecido Ferre, Juan Franco Lemos, Manoel Victor [UNESP] Desiderio, Janete Apparecida [UNESP] Davolos, Camila Chiaradia [UNESP] Brunini Crialesi Legori, Paula Cristina [UNESP] Fernandes, Odair Aparecido Ferre, Juan Franco Lemos, Manoel Victor [UNESP] Desiderio, Janete Apparecida [UNESP] |
author_role |
author |
author2 |
Davolos, Camila Chiaradia [UNESP] Brunini Crialesi Legori, Paula Cristina [UNESP] Fernandes, Odair Aparecido Ferre, Juan Franco Lemos, Manoel Victor [UNESP] Desiderio, Janete Apparecida [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Dept Fitossanidade Univ Valencia |
dc.contributor.author.fl_str_mv |
Nunes Lemes, Ana Rita [UNESP] Davolos, Camila Chiaradia [UNESP] Brunini Crialesi Legori, Paula Cristina [UNESP] Fernandes, Odair Aparecido Ferre, Juan Franco Lemos, Manoel Victor [UNESP] Desiderio, Janete Apparecida [UNESP] |
description |
Second generation Bt crops (insect resistant crops carrying Bacillus thuringiensis genes) combine more than one gene that codes for insecticidal proteins in the same plant to provide better control of agricultural pests. Some of the new combinations involve co-expression of cry and vip genes. Because Cry and Vip proteins have different midgut targets and possibly different mechanisms of toxicity, it is important to evaluate possible synergistic or antagonistic interactions between these two classes of toxins. Three members of the Cry1 class of proteins and three from the Vip3A class were tested against Heliothis virescens for possible interactions. At the level of LC50, Cry1Ac was the most active protein, whereas the rest of proteins tested were similarly active. However, at the level of LC90, Cry1Aa and Cry1Ca were the least active proteins, and Cry1Ac and Vip3A proteins were not significantly different. Under the experimental conditions used in this study, we found an antagonistic effect of Cry1Ca with the three Vip3A proteins. The interaction between Cry1Ca and Vip3Aa was also tested on two other species of Lepidoptera. Whereas antagonism was observed in Spodoptera frugiperda, synergism was found in Diatraea saccharalis. In all cases, the interaction between Vip3A and Cry1 proteins was more evident at the LC90 level than at the LC50 level. The fact that the same combination of proteins may result in a synergistic or an antagonistic interaction may be an indication that there are different types of interactions within the host, depending on the insect species tested. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-10-02 2015-03-18T15:56:04Z 2015-03-18T15:56:04Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1371/journal.pone.0107196 Plos One. San Francisco: Public Library Science, v. 9, n. 10, 8 p., 2014. 1932-6203 http://hdl.handle.net/11449/117408 10.1371/journal.pone.0107196 WOS:000342591500006 WOS000342591500006.pdf 1458288287757880 7179273060624761 1295051340591836 0000-0003-3489-4754 |
url |
http://dx.doi.org/10.1371/journal.pone.0107196 http://hdl.handle.net/11449/117408 |
identifier_str_mv |
Plos One. San Francisco: Public Library Science, v. 9, n. 10, 8 p., 2014. 1932-6203 10.1371/journal.pone.0107196 WOS:000342591500006 WOS000342591500006.pdf 1458288287757880 7179273060624761 1295051340591836 0000-0003-3489-4754 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Plos One 2.766 1,164 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
dc.format.none.fl_str_mv |
8 application/pdf |
dc.publisher.none.fl_str_mv |
Public Library Science |
publisher.none.fl_str_mv |
Public Library Science |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
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Repositório Institucional da UNESP |
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Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1822182293281701888 |
dc.identifier.doi.none.fl_str_mv |
10.1371/journal.pone.0107196 |