Multilocus sequence typing of Dientamoeba fragilis identified a major clone with widespread geographical distribution
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.ijpara.2016.07.002 http://hdl.handle.net/11449/159190 |
Resumo: | The flagellated protozoan Dientamoeba fragilis is often detected in humans with gastrointestinal symptoms, but it is also commonly found in healthy subjects. As for other intestinal protozoa, the hypothesis that genetically dissimilar parasite isolates differ in their ability to cause symptoms has also been raised for D. fragilis. To date, only two D. fragilis genotypes (1 and 2) have been described, of which genotype 1 largely predominates worldwide. However, very few markers are available for genotyping studies and therefore the extent of genetic variation among isolates remains largely unknown. Here, we performed metagenomics experiments on two D. fragilis-positive stool samples, and identified a number of candidate markers based on sequence similarity to the phylogenetically related species Trichomonas vaginalis. Markers corresponding to structural genes and to genes encoding for proteases were selected for this study, and PCR experiments confirmed their belonging to the D. fragilis genome; two previously described markers (small subunit ribosomal DNA and large subunit of RNA polymerase II) were also included. Using this panel of markers, 111 isolates of human origin were genotyped, all of which, except one, belonged to genotype 1. These isolates had been collected at different times from symptomatic and asymptomatic persons of different age groups in Italy, Denmark, Brazil and Australia. By sequencing approximately 160 kb from 500 PCR products, a very low level of polymorphism was observed across all the investigated loci, suggesting the existence of a major clone of D. fragilis with a widespread geographical distribution. (C) 2016 Australian Society for Parasitology. Published by Elsevier Ltd. All rights reserved. |
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Multilocus sequence typing of Dientamoeba fragilis identified a major clone with widespread geographical distributionDientamoeba fragilisHumanGenetic markersMultilocus genotypingPopulation structureThe flagellated protozoan Dientamoeba fragilis is often detected in humans with gastrointestinal symptoms, but it is also commonly found in healthy subjects. As for other intestinal protozoa, the hypothesis that genetically dissimilar parasite isolates differ in their ability to cause symptoms has also been raised for D. fragilis. To date, only two D. fragilis genotypes (1 and 2) have been described, of which genotype 1 largely predominates worldwide. However, very few markers are available for genotyping studies and therefore the extent of genetic variation among isolates remains largely unknown. Here, we performed metagenomics experiments on two D. fragilis-positive stool samples, and identified a number of candidate markers based on sequence similarity to the phylogenetically related species Trichomonas vaginalis. Markers corresponding to structural genes and to genes encoding for proteases were selected for this study, and PCR experiments confirmed their belonging to the D. fragilis genome; two previously described markers (small subunit ribosomal DNA and large subunit of RNA polymerase II) were also included. Using this panel of markers, 111 isolates of human origin were genotyped, all of which, except one, belonged to genotype 1. These isolates had been collected at different times from symptomatic and asymptomatic persons of different age groups in Italy, Denmark, Brazil and Australia. By sequencing approximately 160 kb from 500 PCR products, a very low level of polymorphism was observed across all the investigated loci, suggesting the existence of a major clone of D. fragilis with a widespread geographical distribution. (C) 2016 Australian Society for Parasitology. Published by Elsevier Ltd. All rights reserved.Italian Ministry of Health (Ricerca Corrente)DG SANTE of the European CommissionIst Super Sanita, Rome, ItalyIRCCS San Matteo Hosp Fdn, Lab Parasitol Microbiol & Virol, Pavia, ItalyStatens Serum Inst, Dept Microbiol & Infect Control, Copenhagen, DenmarkSao Paulo State Univ, Inst Biosci, Dept Parasitol, Sao Paulo, BrazilIst Zooprofilatt Sperimentale Umbria & Marche, Parasitol Lab, Perugia, ItalyWestern Diagnost Pathol, Myaree, WA, AustraliaSao Paulo State Univ, Inst Biosci, Dept Parasitol, Sao Paulo, BrazilItalian Ministry of Health (Ricerca Corrente): RC04/12Elsevier B.V.Ist Super SanitaIRCCS San Matteo Hosp FdnStatens Serum InstUniversidade Estadual Paulista (Unesp)Ist Zooprofilatt Sperimentale Umbria & MarcheWestern Diagnost PatholCaccio, Simone M.Sannella, Anna RosaBruno, AntonellaStensvold, Christen R.David, Erica Boarato [UNESP]Guimaraes, Semiramis [UNESP]Manuali, ElisabettaMagistrali, ChiaraMahdad, KarimBeaman, MilesMaserati, RobertaTosini, FabioPozio, Edoardo2018-11-26T15:31:44Z2018-11-26T15:31:44Z2016-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article793-798application/pdfhttp://dx.doi.org/10.1016/j.ijpara.2016.07.002International Journal For Parasitology. Oxford: Elsevier Sci Ltd, v. 46, n. 12, p. 793-798, 2016.0020-7519http://hdl.handle.net/11449/15919010.1016/j.ijpara.2016.07.002WOS:000387630100004WOS000387630100004.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInternational Journal For Parasitology1,638info:eu-repo/semantics/openAccess2024-01-04T06:22:38Zoai:repositorio.unesp.br:11449/159190Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-01-04T06:22:38Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Multilocus sequence typing of Dientamoeba fragilis identified a major clone with widespread geographical distribution |
title |
Multilocus sequence typing of Dientamoeba fragilis identified a major clone with widespread geographical distribution |
spellingShingle |
Multilocus sequence typing of Dientamoeba fragilis identified a major clone with widespread geographical distribution Caccio, Simone M. Dientamoeba fragilis Human Genetic markers Multilocus genotyping Population structure |
title_short |
Multilocus sequence typing of Dientamoeba fragilis identified a major clone with widespread geographical distribution |
title_full |
Multilocus sequence typing of Dientamoeba fragilis identified a major clone with widespread geographical distribution |
title_fullStr |
Multilocus sequence typing of Dientamoeba fragilis identified a major clone with widespread geographical distribution |
title_full_unstemmed |
Multilocus sequence typing of Dientamoeba fragilis identified a major clone with widespread geographical distribution |
title_sort |
Multilocus sequence typing of Dientamoeba fragilis identified a major clone with widespread geographical distribution |
author |
Caccio, Simone M. |
author_facet |
Caccio, Simone M. Sannella, Anna Rosa Bruno, Antonella Stensvold, Christen R. David, Erica Boarato [UNESP] Guimaraes, Semiramis [UNESP] Manuali, Elisabetta Magistrali, Chiara Mahdad, Karim Beaman, Miles Maserati, Roberta Tosini, Fabio Pozio, Edoardo |
author_role |
author |
author2 |
Sannella, Anna Rosa Bruno, Antonella Stensvold, Christen R. David, Erica Boarato [UNESP] Guimaraes, Semiramis [UNESP] Manuali, Elisabetta Magistrali, Chiara Mahdad, Karim Beaman, Miles Maserati, Roberta Tosini, Fabio Pozio, Edoardo |
author2_role |
author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Ist Super Sanita IRCCS San Matteo Hosp Fdn Statens Serum Inst Universidade Estadual Paulista (Unesp) Ist Zooprofilatt Sperimentale Umbria & Marche Western Diagnost Pathol |
dc.contributor.author.fl_str_mv |
Caccio, Simone M. Sannella, Anna Rosa Bruno, Antonella Stensvold, Christen R. David, Erica Boarato [UNESP] Guimaraes, Semiramis [UNESP] Manuali, Elisabetta Magistrali, Chiara Mahdad, Karim Beaman, Miles Maserati, Roberta Tosini, Fabio Pozio, Edoardo |
dc.subject.por.fl_str_mv |
Dientamoeba fragilis Human Genetic markers Multilocus genotyping Population structure |
topic |
Dientamoeba fragilis Human Genetic markers Multilocus genotyping Population structure |
description |
The flagellated protozoan Dientamoeba fragilis is often detected in humans with gastrointestinal symptoms, but it is also commonly found in healthy subjects. As for other intestinal protozoa, the hypothesis that genetically dissimilar parasite isolates differ in their ability to cause symptoms has also been raised for D. fragilis. To date, only two D. fragilis genotypes (1 and 2) have been described, of which genotype 1 largely predominates worldwide. However, very few markers are available for genotyping studies and therefore the extent of genetic variation among isolates remains largely unknown. Here, we performed metagenomics experiments on two D. fragilis-positive stool samples, and identified a number of candidate markers based on sequence similarity to the phylogenetically related species Trichomonas vaginalis. Markers corresponding to structural genes and to genes encoding for proteases were selected for this study, and PCR experiments confirmed their belonging to the D. fragilis genome; two previously described markers (small subunit ribosomal DNA and large subunit of RNA polymerase II) were also included. Using this panel of markers, 111 isolates of human origin were genotyped, all of which, except one, belonged to genotype 1. These isolates had been collected at different times from symptomatic and asymptomatic persons of different age groups in Italy, Denmark, Brazil and Australia. By sequencing approximately 160 kb from 500 PCR products, a very low level of polymorphism was observed across all the investigated loci, suggesting the existence of a major clone of D. fragilis with a widespread geographical distribution. (C) 2016 Australian Society for Parasitology. Published by Elsevier Ltd. All rights reserved. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-11-01 2018-11-26T15:31:44Z 2018-11-26T15:31:44Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.ijpara.2016.07.002 International Journal For Parasitology. Oxford: Elsevier Sci Ltd, v. 46, n. 12, p. 793-798, 2016. 0020-7519 http://hdl.handle.net/11449/159190 10.1016/j.ijpara.2016.07.002 WOS:000387630100004 WOS000387630100004.pdf |
url |
http://dx.doi.org/10.1016/j.ijpara.2016.07.002 http://hdl.handle.net/11449/159190 |
identifier_str_mv |
International Journal For Parasitology. Oxford: Elsevier Sci Ltd, v. 46, n. 12, p. 793-798, 2016. 0020-7519 10.1016/j.ijpara.2016.07.002 WOS:000387630100004 WOS000387630100004.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
International Journal For Parasitology 1,638 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
793-798 application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier B.V. |
publisher.none.fl_str_mv |
Elsevier B.V. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1803650164254048256 |