Myotendinous junction adaptations to ladder-based resistance training: identification of a new telocyte niche
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1038/s41598-020-70971-6 http://hdl.handle.net/11449/199289 |
Resumo: | The present study shows chronic adjustments in the myotendinous junction (MTJ) in response to different ladder-based resistance training (LRT) protocols. Thirty adult male Wistar rats were divided into groups: sedentary (S), calisthenics (LRT without additional load [C]), and resistance-trained (LRT with extra weight [R]). We demonstrated longer lengths of sarcoplasmatic invaginations in the trained groups; however, evaginations were seen mainly in group R. We showed a greater thickness of sarcoplasmatic invaginations in groups C and R, in addition to greater evaginations in R. We also observed thinner basal lamina in trained groups. The support collagen layer (SCL) adjacent to the MTJ and the diameters of the transverse fibrils were larger in R. We also discovered a niche of telocytes in the MTJ with electron micrographs of the plantar muscle and with immunostaining with CD34+ in the gastrocnemius muscle near the blood vessels and pericytes. We concluded that the continuous adjustments in the MTJ ultrastructure were the result of tissue plasticity induced by LRT, which is causally related to muscle hypertrophy and, consequently, to the remodeling of the contact interface. Also, we reveal the existence of a collagen layer adjacent to MTJ and discover a new micro anatomic location of telocytes. |
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spelling |
Myotendinous junction adaptations to ladder-based resistance training: identification of a new telocyte nicheThe present study shows chronic adjustments in the myotendinous junction (MTJ) in response to different ladder-based resistance training (LRT) protocols. Thirty adult male Wistar rats were divided into groups: sedentary (S), calisthenics (LRT without additional load [C]), and resistance-trained (LRT with extra weight [R]). We demonstrated longer lengths of sarcoplasmatic invaginations in the trained groups; however, evaginations were seen mainly in group R. We showed a greater thickness of sarcoplasmatic invaginations in groups C and R, in addition to greater evaginations in R. We also observed thinner basal lamina in trained groups. The support collagen layer (SCL) adjacent to the MTJ and the diameters of the transverse fibrils were larger in R. We also discovered a niche of telocytes in the MTJ with electron micrographs of the plantar muscle and with immunostaining with CD34+ in the gastrocnemius muscle near the blood vessels and pericytes. We concluded that the continuous adjustments in the MTJ ultrastructure were the result of tissue plasticity induced by LRT, which is causally related to muscle hypertrophy and, consequently, to the remodeling of the contact interface. Also, we reveal the existence of a collagen layer adjacent to MTJ and discover a new micro anatomic location of telocytes.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Laboratory of Morphology and Physical Activity (LAMAF) Institute of Biosciences (IB) São Paulo State University “Júlio de Mesquita Filho”-UNESPLaboratory of Morphoquantitative Studies and Immunohistochemistry Department of Physical Education São Judas Tadeu University (USJT)Department of Anatomy,ICB-III University of São Paulo-USPLaboratory of Morphology and Physical Activity (LAMAF) Institute of Biosciences (IB) São Paulo State University “Júlio de Mesquita Filho”-UNESPFAPESP: 2017/12525-1FAPESP: 2017/21977-3FAPESP: 2018/09199-8Universidade Estadual Paulista (Unesp)São Judas Tadeu University (USJT)Universidade de São Paulo (USP)Pimentel Neto, Jurandyr [UNESP]Rocha, Lara Caetano [UNESP]Barbosa, Gabriela Klein [UNESP]Jacob, Carolina dos Santos [UNESP]Krause Neto, WalterWatanabe, Ii-seiCiena, Adriano Polican [UNESP]2020-12-12T01:35:49Z2020-12-12T01:35:49Z2020-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1038/s41598-020-70971-6Scientific Reports, v. 10, n. 1, 2020.2045-2322http://hdl.handle.net/11449/19928910.1038/s41598-020-70971-62-s2.0-85089752885Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengScientific Reportsinfo:eu-repo/semantics/openAccess2021-10-23T06:52:14Zoai:repositorio.unesp.br:11449/199289Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T06:52:14Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Myotendinous junction adaptations to ladder-based resistance training: identification of a new telocyte niche |
title |
Myotendinous junction adaptations to ladder-based resistance training: identification of a new telocyte niche |
spellingShingle |
Myotendinous junction adaptations to ladder-based resistance training: identification of a new telocyte niche Pimentel Neto, Jurandyr [UNESP] |
title_short |
Myotendinous junction adaptations to ladder-based resistance training: identification of a new telocyte niche |
title_full |
Myotendinous junction adaptations to ladder-based resistance training: identification of a new telocyte niche |
title_fullStr |
Myotendinous junction adaptations to ladder-based resistance training: identification of a new telocyte niche |
title_full_unstemmed |
Myotendinous junction adaptations to ladder-based resistance training: identification of a new telocyte niche |
title_sort |
Myotendinous junction adaptations to ladder-based resistance training: identification of a new telocyte niche |
author |
Pimentel Neto, Jurandyr [UNESP] |
author_facet |
Pimentel Neto, Jurandyr [UNESP] Rocha, Lara Caetano [UNESP] Barbosa, Gabriela Klein [UNESP] Jacob, Carolina dos Santos [UNESP] Krause Neto, Walter Watanabe, Ii-sei Ciena, Adriano Polican [UNESP] |
author_role |
author |
author2 |
Rocha, Lara Caetano [UNESP] Barbosa, Gabriela Klein [UNESP] Jacob, Carolina dos Santos [UNESP] Krause Neto, Walter Watanabe, Ii-sei Ciena, Adriano Polican [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) São Judas Tadeu University (USJT) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Pimentel Neto, Jurandyr [UNESP] Rocha, Lara Caetano [UNESP] Barbosa, Gabriela Klein [UNESP] Jacob, Carolina dos Santos [UNESP] Krause Neto, Walter Watanabe, Ii-sei Ciena, Adriano Polican [UNESP] |
description |
The present study shows chronic adjustments in the myotendinous junction (MTJ) in response to different ladder-based resistance training (LRT) protocols. Thirty adult male Wistar rats were divided into groups: sedentary (S), calisthenics (LRT without additional load [C]), and resistance-trained (LRT with extra weight [R]). We demonstrated longer lengths of sarcoplasmatic invaginations in the trained groups; however, evaginations were seen mainly in group R. We showed a greater thickness of sarcoplasmatic invaginations in groups C and R, in addition to greater evaginations in R. We also observed thinner basal lamina in trained groups. The support collagen layer (SCL) adjacent to the MTJ and the diameters of the transverse fibrils were larger in R. We also discovered a niche of telocytes in the MTJ with electron micrographs of the plantar muscle and with immunostaining with CD34+ in the gastrocnemius muscle near the blood vessels and pericytes. We concluded that the continuous adjustments in the MTJ ultrastructure were the result of tissue plasticity induced by LRT, which is causally related to muscle hypertrophy and, consequently, to the remodeling of the contact interface. Also, we reveal the existence of a collagen layer adjacent to MTJ and discover a new micro anatomic location of telocytes. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T01:35:49Z 2020-12-12T01:35:49Z 2020-12-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1038/s41598-020-70971-6 Scientific Reports, v. 10, n. 1, 2020. 2045-2322 http://hdl.handle.net/11449/199289 10.1038/s41598-020-70971-6 2-s2.0-85089752885 |
url |
http://dx.doi.org/10.1038/s41598-020-70971-6 http://hdl.handle.net/11449/199289 |
identifier_str_mv |
Scientific Reports, v. 10, n. 1, 2020. 2045-2322 10.1038/s41598-020-70971-6 2-s2.0-85089752885 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Scientific Reports |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1803650182882000896 |