Impact of the medicine Hydroxyurea on reproduction and development, using Drosophila melanogaster as a model organism
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://benthamopen.com/ABSTRACT/TOPHJ-7-16 http://hdl.handle.net/11449/122476 |
Resumo: | The clinical efficacy of oral hydroxyurea (HU) in the treatment of the sickle cell disease (SCD) is already proven. However, side-effects on reproduction and development still cause concerns, mainly in long term treatments. We used Drosophila melanogaster as a model organism in the study of this subject. This species has been considered favorable for studying human diseases and responses to drugs because both organisms share more than 50% of the genes for disease and exhibit a very similar drug metabolism. In addition, D. melanogaster allows laboratory approaches that are not possible in humans. We analyzed the impact of the concentrations HU 0.1 and HU 0.25 on productivity, oviposition rate, emergence period duration, mortality and development time from egg to adult. HU affected all these traits, showing dose dependence. The frequency of mating, and duration of premating and mating times of flies were also analyzed in treatments with HU 0.25. Considering all the traits, the treatments decreased the productivity, oviposition rate and frequency of mating, and increased the emergence period duration, mortality rate from egg to adult and development time. The premating and mating times duration were affected in a more complex way. On the basis of the knowledge that HU affects DNA synthesis and repair, and other data available in the literature, we raised hypotheses to explain the present observations. The results and hypotheses suggested new approaches for further studies of particular and important aspects. In general, this study reinforces the validity of the concern with HU side-effects. |
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Impact of the medicine Hydroxyurea on reproduction and development, using Drosophila melanogaster as a model organismEmergence period durationimpaired effect of hydroxyureamortality from egg to adultnumber of progenyoviposition rateThe clinical efficacy of oral hydroxyurea (HU) in the treatment of the sickle cell disease (SCD) is already proven. However, side-effects on reproduction and development still cause concerns, mainly in long term treatments. We used Drosophila melanogaster as a model organism in the study of this subject. This species has been considered favorable for studying human diseases and responses to drugs because both organisms share more than 50% of the genes for disease and exhibit a very similar drug metabolism. In addition, D. melanogaster allows laboratory approaches that are not possible in humans. We analyzed the impact of the concentrations HU 0.1 and HU 0.25 on productivity, oviposition rate, emergence period duration, mortality and development time from egg to adult. HU affected all these traits, showing dose dependence. The frequency of mating, and duration of premating and mating times of flies were also analyzed in treatments with HU 0.25. Considering all the traits, the treatments decreased the productivity, oviposition rate and frequency of mating, and increased the emergence period duration, mortality rate from egg to adult and development time. The premating and mating times duration were affected in a more complex way. On the basis of the knowledge that HU affects DNA synthesis and repair, and other data available in the literature, we raised hypotheses to explain the present observations. The results and hypotheses suggested new approaches for further studies of particular and important aspects. In general, this study reinforces the validity of the concern with HU side-effects.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento Pessoal de Nível Superior (CAPES)Universidade Estadual Paulista Júlio de Mesquita Filho, Departamento de Biologia, Instituto de Biociencias Letras e Ciencias Exatas de Sao Jose do Rio PretoUniversidade Estadual Paulista Júlio de Mesquita Filho, Departamento de Computação, Instituto de Biociencias Letras e Ciencias Exatas de Sao Jose do Rio PretoUniversidade Estadual Paulista (Unesp)Rangel, Veronica Ferreira [UNESP]Patarro, Thais de França [UNESP]Manzato, Antonio José [UNESP]Bicudo, Hermione Elly Melara de Campos [UNESP]2015-04-27T11:55:47Z2015-04-27T11:55:47Z2014info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article16-23application/pdfhttp://benthamopen.com/ABSTRACT/TOPHJ-7-16Open Public Health Journal, v. 7, p. 16-23, 2014.1874-9445http://hdl.handle.net/11449/12247610.2174/1874944501407010016ISSN1874-9445-2014-07-16-23.pdf74563561793477316200303968181270Currículo Lattesreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengOpen Public Health Journal0,102info:eu-repo/semantics/openAccess2023-12-03T06:17:49Zoai:repositorio.unesp.br:11449/122476Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-12-03T06:17:49Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Impact of the medicine Hydroxyurea on reproduction and development, using Drosophila melanogaster as a model organism |
title |
Impact of the medicine Hydroxyurea on reproduction and development, using Drosophila melanogaster as a model organism |
spellingShingle |
Impact of the medicine Hydroxyurea on reproduction and development, using Drosophila melanogaster as a model organism Rangel, Veronica Ferreira [UNESP] Emergence period duration impaired effect of hydroxyurea mortality from egg to adult number of progeny oviposition rate |
title_short |
Impact of the medicine Hydroxyurea on reproduction and development, using Drosophila melanogaster as a model organism |
title_full |
Impact of the medicine Hydroxyurea on reproduction and development, using Drosophila melanogaster as a model organism |
title_fullStr |
Impact of the medicine Hydroxyurea on reproduction and development, using Drosophila melanogaster as a model organism |
title_full_unstemmed |
Impact of the medicine Hydroxyurea on reproduction and development, using Drosophila melanogaster as a model organism |
title_sort |
Impact of the medicine Hydroxyurea on reproduction and development, using Drosophila melanogaster as a model organism |
author |
Rangel, Veronica Ferreira [UNESP] |
author_facet |
Rangel, Veronica Ferreira [UNESP] Patarro, Thais de França [UNESP] Manzato, Antonio José [UNESP] Bicudo, Hermione Elly Melara de Campos [UNESP] |
author_role |
author |
author2 |
Patarro, Thais de França [UNESP] Manzato, Antonio José [UNESP] Bicudo, Hermione Elly Melara de Campos [UNESP] |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Rangel, Veronica Ferreira [UNESP] Patarro, Thais de França [UNESP] Manzato, Antonio José [UNESP] Bicudo, Hermione Elly Melara de Campos [UNESP] |
dc.subject.por.fl_str_mv |
Emergence period duration impaired effect of hydroxyurea mortality from egg to adult number of progeny oviposition rate |
topic |
Emergence period duration impaired effect of hydroxyurea mortality from egg to adult number of progeny oviposition rate |
description |
The clinical efficacy of oral hydroxyurea (HU) in the treatment of the sickle cell disease (SCD) is already proven. However, side-effects on reproduction and development still cause concerns, mainly in long term treatments. We used Drosophila melanogaster as a model organism in the study of this subject. This species has been considered favorable for studying human diseases and responses to drugs because both organisms share more than 50% of the genes for disease and exhibit a very similar drug metabolism. In addition, D. melanogaster allows laboratory approaches that are not possible in humans. We analyzed the impact of the concentrations HU 0.1 and HU 0.25 on productivity, oviposition rate, emergence period duration, mortality and development time from egg to adult. HU affected all these traits, showing dose dependence. The frequency of mating, and duration of premating and mating times of flies were also analyzed in treatments with HU 0.25. Considering all the traits, the treatments decreased the productivity, oviposition rate and frequency of mating, and increased the emergence period duration, mortality rate from egg to adult and development time. The premating and mating times duration were affected in a more complex way. On the basis of the knowledge that HU affects DNA synthesis and repair, and other data available in the literature, we raised hypotheses to explain the present observations. The results and hypotheses suggested new approaches for further studies of particular and important aspects. In general, this study reinforces the validity of the concern with HU side-effects. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014 2015-04-27T11:55:47Z 2015-04-27T11:55:47Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://benthamopen.com/ABSTRACT/TOPHJ-7-16 Open Public Health Journal, v. 7, p. 16-23, 2014. 1874-9445 http://hdl.handle.net/11449/122476 10.2174/1874944501407010016 ISSN1874-9445-2014-07-16-23.pdf 7456356179347731 6200303968181270 |
url |
http://benthamopen.com/ABSTRACT/TOPHJ-7-16 http://hdl.handle.net/11449/122476 |
identifier_str_mv |
Open Public Health Journal, v. 7, p. 16-23, 2014. 1874-9445 10.2174/1874944501407010016 ISSN1874-9445-2014-07-16-23.pdf 7456356179347731 6200303968181270 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Open Public Health Journal 0,102 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
16-23 application/pdf |
dc.source.none.fl_str_mv |
Currículo Lattes reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965161273950208 |