Screening of Bacillus for the development of bioprotectants for the control of Fusarium oxysporum f. sp. vasinfectum and meloidogye incognita
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.biocontrol.2021.104764 http://hdl.handle.net/11449/229553 |
Resumo: | Fusarium oxysporum f. sp. vasinfectum and Meloidogyne incognita cause large losses in cotton productivity. These pathogens can occur simultaneously, resulting in a Fusarium-nematode complex, which strengthens the destructive power when compared to the pathogens alone. Due to the ineffectiveness of chemicals, and to the fact that chemicals pesticides are responsible for releasing residues into the environment, more attention has been given to biocontrol. Bacillus are recognized by their ability to control plant-parasitic pathogens. However, since Fusarium and Meloidogyne often occur simultaneously, and the resulting complex causes more losses than each pathogen alone, selecting bioagents that act against both pathogens simultaneously is important, and this is the objective of this study. Among 40 Bacillus strains obtained from cotton rhizosphere and other sites, the most efficient ones were selected to inhibit mycelial growth and spore germination of Fusarium, cause J2 mortality and inhibit hatching of M. incognita. Forty Bacillus strains were evaluated both through biochemical tests related to plant growth promotion (indoleacetic acid, siderophores, and cyanhydric acid production; catalase activities; nitrogen fixation and phosphate solubilization) and root colonization. Strains S2531, S2535, S2545, S2548, S2552, S2554, S2555, and S2557 showed >4 positive responses for biochemical tests. Strains S2527, S2535, S2538, S2545, S2573, AP03, AP115, AP117, AP210, QST713 colonized cotton roots. Strains S2535, S2543, S2545, S2557, S2560, S2564, AP03, AP10, AP115, and AP117 inhibited mycelial growth of Fusarium. The most efficient strains for the inhibition of microconidia germination were S2545, S2548, AP115, AP03, and S2543. Strains AP117, S2527, S2530, S2538, S2560, and S2569 showed the highest mortality of J2. Strains S2527, S2557, S2569 and AP03 were the ones with the lowest area under hatching progress curve. Overall, strains AP03, S2527 and S2545 were selected to evaluate the control of the complex Fusarium-nematodes in cotton plants. These three strains were identified as Bacillus velezensis. |
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Screening of Bacillus for the development of bioprotectants for the control of Fusarium oxysporum f. sp. vasinfectum and meloidogye incognitaBacillusBioprotectantsCottonFusarium oxysporum f. sp. vasinfectumMeloidogyne incognitaScreeningFusarium oxysporum f. sp. vasinfectum and Meloidogyne incognita cause large losses in cotton productivity. These pathogens can occur simultaneously, resulting in a Fusarium-nematode complex, which strengthens the destructive power when compared to the pathogens alone. Due to the ineffectiveness of chemicals, and to the fact that chemicals pesticides are responsible for releasing residues into the environment, more attention has been given to biocontrol. Bacillus are recognized by their ability to control plant-parasitic pathogens. However, since Fusarium and Meloidogyne often occur simultaneously, and the resulting complex causes more losses than each pathogen alone, selecting bioagents that act against both pathogens simultaneously is important, and this is the objective of this study. Among 40 Bacillus strains obtained from cotton rhizosphere and other sites, the most efficient ones were selected to inhibit mycelial growth and spore germination of Fusarium, cause J2 mortality and inhibit hatching of M. incognita. Forty Bacillus strains were evaluated both through biochemical tests related to plant growth promotion (indoleacetic acid, siderophores, and cyanhydric acid production; catalase activities; nitrogen fixation and phosphate solubilization) and root colonization. Strains S2531, S2535, S2545, S2548, S2552, S2554, S2555, and S2557 showed >4 positive responses for biochemical tests. Strains S2527, S2535, S2538, S2545, S2573, AP03, AP115, AP117, AP210, QST713 colonized cotton roots. Strains S2535, S2543, S2545, S2557, S2560, S2564, AP03, AP10, AP115, and AP117 inhibited mycelial growth of Fusarium. The most efficient strains for the inhibition of microconidia germination were S2545, S2548, AP115, AP03, and S2543. Strains AP117, S2527, S2530, S2538, S2560, and S2569 showed the highest mortality of J2. Strains S2527, S2557, S2569 and AP03 were the ones with the lowest area under hatching progress curve. Overall, strains AP03, S2527 and S2545 were selected to evaluate the control of the complex Fusarium-nematodes in cotton plants. These three strains were identified as Bacillus velezensis.Universidade Estadual Paulista “Júlio de Mesquita Filho” Faculdade de Ciências Agronômicas Campus Fazenda Lageado Departamento de Proteção VegetalEmbrapa Embrapa Recursos Genéticos e Biotecnologia CEP 70770-917Embrapa Meio Ambiente, Rod. SP-340 Km 127, 5Universidade Estadual Paulista “Júlio de Mesquita Filho” Faculdade de Ciências Agronômicas Campus Fazenda Lageado Departamento de Proteção VegetalUniversidade Estadual Paulista (UNESP)Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)Guimarães Pacifico, Marina [UNESP]Eckstein, BárbaraBettiol, Wagner2022-04-29T08:33:12Z2022-04-29T08:33:12Z2021-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.biocontrol.2021.104764Biological Control, v. 164.1049-9644http://hdl.handle.net/11449/22955310.1016/j.biocontrol.2021.1047642-s2.0-85115290732Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBiological Controlinfo:eu-repo/semantics/openAccess2024-04-30T18:07:33Zoai:repositorio.unesp.br:11449/229553Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-04-30T18:07:33Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Screening of Bacillus for the development of bioprotectants for the control of Fusarium oxysporum f. sp. vasinfectum and meloidogye incognita |
title |
Screening of Bacillus for the development of bioprotectants for the control of Fusarium oxysporum f. sp. vasinfectum and meloidogye incognita |
spellingShingle |
Screening of Bacillus for the development of bioprotectants for the control of Fusarium oxysporum f. sp. vasinfectum and meloidogye incognita Guimarães Pacifico, Marina [UNESP] Bacillus Bioprotectants Cotton Fusarium oxysporum f. sp. vasinfectum Meloidogyne incognita Screening |
title_short |
Screening of Bacillus for the development of bioprotectants for the control of Fusarium oxysporum f. sp. vasinfectum and meloidogye incognita |
title_full |
Screening of Bacillus for the development of bioprotectants for the control of Fusarium oxysporum f. sp. vasinfectum and meloidogye incognita |
title_fullStr |
Screening of Bacillus for the development of bioprotectants for the control of Fusarium oxysporum f. sp. vasinfectum and meloidogye incognita |
title_full_unstemmed |
Screening of Bacillus for the development of bioprotectants for the control of Fusarium oxysporum f. sp. vasinfectum and meloidogye incognita |
title_sort |
Screening of Bacillus for the development of bioprotectants for the control of Fusarium oxysporum f. sp. vasinfectum and meloidogye incognita |
author |
Guimarães Pacifico, Marina [UNESP] |
author_facet |
Guimarães Pacifico, Marina [UNESP] Eckstein, Bárbara Bettiol, Wagner |
author_role |
author |
author2 |
Eckstein, Bárbara Bettiol, Wagner |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) |
dc.contributor.author.fl_str_mv |
Guimarães Pacifico, Marina [UNESP] Eckstein, Bárbara Bettiol, Wagner |
dc.subject.por.fl_str_mv |
Bacillus Bioprotectants Cotton Fusarium oxysporum f. sp. vasinfectum Meloidogyne incognita Screening |
topic |
Bacillus Bioprotectants Cotton Fusarium oxysporum f. sp. vasinfectum Meloidogyne incognita Screening |
description |
Fusarium oxysporum f. sp. vasinfectum and Meloidogyne incognita cause large losses in cotton productivity. These pathogens can occur simultaneously, resulting in a Fusarium-nematode complex, which strengthens the destructive power when compared to the pathogens alone. Due to the ineffectiveness of chemicals, and to the fact that chemicals pesticides are responsible for releasing residues into the environment, more attention has been given to biocontrol. Bacillus are recognized by their ability to control plant-parasitic pathogens. However, since Fusarium and Meloidogyne often occur simultaneously, and the resulting complex causes more losses than each pathogen alone, selecting bioagents that act against both pathogens simultaneously is important, and this is the objective of this study. Among 40 Bacillus strains obtained from cotton rhizosphere and other sites, the most efficient ones were selected to inhibit mycelial growth and spore germination of Fusarium, cause J2 mortality and inhibit hatching of M. incognita. Forty Bacillus strains were evaluated both through biochemical tests related to plant growth promotion (indoleacetic acid, siderophores, and cyanhydric acid production; catalase activities; nitrogen fixation and phosphate solubilization) and root colonization. Strains S2531, S2535, S2545, S2548, S2552, S2554, S2555, and S2557 showed >4 positive responses for biochemical tests. Strains S2527, S2535, S2538, S2545, S2573, AP03, AP115, AP117, AP210, QST713 colonized cotton roots. Strains S2535, S2543, S2545, S2557, S2560, S2564, AP03, AP10, AP115, and AP117 inhibited mycelial growth of Fusarium. The most efficient strains for the inhibition of microconidia germination were S2545, S2548, AP115, AP03, and S2543. Strains AP117, S2527, S2530, S2538, S2560, and S2569 showed the highest mortality of J2. Strains S2527, S2557, S2569 and AP03 were the ones with the lowest area under hatching progress curve. Overall, strains AP03, S2527 and S2545 were selected to evaluate the control of the complex Fusarium-nematodes in cotton plants. These three strains were identified as Bacillus velezensis. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-12-01 2022-04-29T08:33:12Z 2022-04-29T08:33:12Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.biocontrol.2021.104764 Biological Control, v. 164. 1049-9644 http://hdl.handle.net/11449/229553 10.1016/j.biocontrol.2021.104764 2-s2.0-85115290732 |
url |
http://dx.doi.org/10.1016/j.biocontrol.2021.104764 http://hdl.handle.net/11449/229553 |
identifier_str_mv |
Biological Control, v. 164. 1049-9644 10.1016/j.biocontrol.2021.104764 2-s2.0-85115290732 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Biological Control |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803046970146684928 |