Post-mortem muscle proteome of crossbred bulls and steers: Relationships with carcass and meat quality

Detalhes bibliográficos
Autor(a) principal: Santiago, Bismarck [UNESP]
Data de Publicação: 2023
Outros Autores: Baldassini, Welder [UNESP], Neto, Otávio Machado [UNESP], Chardulo, Luis Artur [UNESP], Torres, Rodrigo [UNESP], Pereira, Guilherme [UNESP], Curi, Rogério [UNESP], Chiaratti, Marcos Roberto, Padilha, Pedro [UNESP], Alessandroni, Laura, Gagaoua, Mohammed
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.jprot.2023.104871
http://hdl.handle.net/11449/247046
Resumo: This study investigated the skeletal muscle proteome of crossbred bulls and steers with the aim of explaining the differences in carcass and meat quality traits. Therefore, 640 post-weaning Angus-Nellore calves were fed a high-energy diet for a period of 180 days. In the feedlot trial, comparisons of steers (n = 320) and bulls (n = 320) showed lower (P < 0.01) average daily gain (1.38 vs. 1.60 ± 0.05 kg/d), final body weight (547.4 vs. 585.1 ± 9.3 kg), which resulted in lower hot carcass weight (298.4 vs. 333.7 ± 7.7 kg) and ribeye area (68.6 vs. 81.0 ± 2.56 cm2). Steers had higher (P < 0.01) carcass fatness, meat color parameters (L*, a*, b*, chroma (C*), hue (h°)) and lower ultimate pH. Moreover, lower (P < 0.01) Warner-Bratzler shear force (WBSF) were observed in steers compared to bulls (WBSF = 3.68 vs. 4.97 ± 0.08 kg; and 3.19 vs. 4.08 ± 0.08 kg). The proteomic approach using two-dimensional electrophoresis, mass spectrometry and bioinformatics procedures revealed several differentially expressed proteins between steers and bulls (P < 0.05). Interconnected pathways and substantial changes were revealed in biological processes, molecular functions, and cellular components between the post-mortem muscle proteomes of the compared animals. Steers had increased (P < 0.05) abundance of proteins related to energy metabolism (CKM, ALDOA, and GAPDH), and bulls had greater abundance of proteins associated with catabolic (glycolysis) processes (PGM1); oxidative stress (HSP60, HSPA8 and GSTP1); and muscle structure and contraction (TNNI2 and TNNT3). The better carcass (fatness and marbling degree) and meat quality traits (tenderness and color parameters) of steers were associated with higher abundance of key proteins of energy metabolism and lower abundance of enzymes related to catabolic processes, oxidative stress, and proteins of muscle contraction Significance: Sexual condition of cattle is known to be an important factor affecting animal performances and growth as well as the carcass and meat quality traits. The investigation of skeletal muscle proteome help a better understanding of the origin of the differences in quality traits between bulls and steers. The inferior meat quality of bulls was found to be due to the greater expression of proteins associated with primary and catabolic processes, oxidative stress, and muscle contraction. Steers had greater expression of proteins, from which several are known biomarkers of beef quality (mainly tenderness).
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spelling Post-mortem muscle proteome of crossbred bulls and steers: Relationships with carcass and meat qualityBeef tendernessFeedlot, meat color, proteomics, biological mechanismsMass spectrometryMeat qualityProteomeThis study investigated the skeletal muscle proteome of crossbred bulls and steers with the aim of explaining the differences in carcass and meat quality traits. Therefore, 640 post-weaning Angus-Nellore calves were fed a high-energy diet for a period of 180 days. In the feedlot trial, comparisons of steers (n = 320) and bulls (n = 320) showed lower (P < 0.01) average daily gain (1.38 vs. 1.60 ± 0.05 kg/d), final body weight (547.4 vs. 585.1 ± 9.3 kg), which resulted in lower hot carcass weight (298.4 vs. 333.7 ± 7.7 kg) and ribeye area (68.6 vs. 81.0 ± 2.56 cm2). Steers had higher (P < 0.01) carcass fatness, meat color parameters (L*, a*, b*, chroma (C*), hue (h°)) and lower ultimate pH. Moreover, lower (P < 0.01) Warner-Bratzler shear force (WBSF) were observed in steers compared to bulls (WBSF = 3.68 vs. 4.97 ± 0.08 kg; and 3.19 vs. 4.08 ± 0.08 kg). The proteomic approach using two-dimensional electrophoresis, mass spectrometry and bioinformatics procedures revealed several differentially expressed proteins between steers and bulls (P < 0.05). Interconnected pathways and substantial changes were revealed in biological processes, molecular functions, and cellular components between the post-mortem muscle proteomes of the compared animals. Steers had increased (P < 0.05) abundance of proteins related to energy metabolism (CKM, ALDOA, and GAPDH), and bulls had greater abundance of proteins associated with catabolic (glycolysis) processes (PGM1); oxidative stress (HSP60, HSPA8 and GSTP1); and muscle structure and contraction (TNNI2 and TNNT3). The better carcass (fatness and marbling degree) and meat quality traits (tenderness and color parameters) of steers were associated with higher abundance of key proteins of energy metabolism and lower abundance of enzymes related to catabolic processes, oxidative stress, and proteins of muscle contraction Significance: Sexual condition of cattle is known to be an important factor affecting animal performances and growth as well as the carcass and meat quality traits. The investigation of skeletal muscle proteome help a better understanding of the origin of the differences in quality traits between bulls and steers. The inferior meat quality of bulls was found to be due to the greater expression of proteins associated with primary and catabolic processes, oxidative stress, and muscle contraction. Steers had greater expression of proteins, from which several are known biomarkers of beef quality (mainly tenderness).Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)School of Agriculture and Veterinary Sciences (FCAV) São Paulo State University (UNESP), São PauloSchool of Veterinary Medicine and Animal Science (FMVZ) São Paulo State University (UNESP), São PauloUniversidade Federal de São Carlos (UFSCar) Departamento de Genética e Evolução, São PauloInstitute of Bioscience (IB) São Paulo State University (UNESP) Departamento de Química e Bioquímica, São PauloChemistry Interdisciplinary Project (CHIP) School of Pharmacy University of Camerino, Via Madonna delle CarceriPEGASE INRAE Institut AgroSchool of Agriculture and Veterinary Sciences (FCAV) São Paulo State University (UNESP), São PauloSchool of Veterinary Medicine and Animal Science (FMVZ) São Paulo State University (UNESP), São PauloInstitute of Bioscience (IB) São Paulo State University (UNESP) Departamento de Química e Bioquímica, São PauloFAPESP: 2018/00981-5FAPESP: 2019/11028-0Universidade Estadual Paulista (UNESP)Universidade Federal de São Carlos (UFSCar)University of CamerinoInstitut AgroSantiago, Bismarck [UNESP]Baldassini, Welder [UNESP]Neto, Otávio Machado [UNESP]Chardulo, Luis Artur [UNESP]Torres, Rodrigo [UNESP]Pereira, Guilherme [UNESP]Curi, Rogério [UNESP]Chiaratti, Marcos RobertoPadilha, Pedro [UNESP]Alessandroni, LauraGagaoua, Mohammed2023-07-29T12:57:34Z2023-07-29T12:57:34Z2023-04-30info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.jprot.2023.104871Journal of Proteomics, v. 278.1876-77371874-3919http://hdl.handle.net/11449/24704610.1016/j.jprot.2023.1048712-s2.0-85150882486Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Proteomicsinfo:eu-repo/semantics/openAccess2023-07-29T12:57:34Zoai:repositorio.unesp.br:11449/247046Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:08:29.408Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Post-mortem muscle proteome of crossbred bulls and steers: Relationships with carcass and meat quality
title Post-mortem muscle proteome of crossbred bulls and steers: Relationships with carcass and meat quality
spellingShingle Post-mortem muscle proteome of crossbred bulls and steers: Relationships with carcass and meat quality
Santiago, Bismarck [UNESP]
Beef tenderness
Feedlot, meat color, proteomics, biological mechanisms
Mass spectrometry
Meat quality
Proteome
title_short Post-mortem muscle proteome of crossbred bulls and steers: Relationships with carcass and meat quality
title_full Post-mortem muscle proteome of crossbred bulls and steers: Relationships with carcass and meat quality
title_fullStr Post-mortem muscle proteome of crossbred bulls and steers: Relationships with carcass and meat quality
title_full_unstemmed Post-mortem muscle proteome of crossbred bulls and steers: Relationships with carcass and meat quality
title_sort Post-mortem muscle proteome of crossbred bulls and steers: Relationships with carcass and meat quality
author Santiago, Bismarck [UNESP]
author_facet Santiago, Bismarck [UNESP]
Baldassini, Welder [UNESP]
Neto, Otávio Machado [UNESP]
Chardulo, Luis Artur [UNESP]
Torres, Rodrigo [UNESP]
Pereira, Guilherme [UNESP]
Curi, Rogério [UNESP]
Chiaratti, Marcos Roberto
Padilha, Pedro [UNESP]
Alessandroni, Laura
Gagaoua, Mohammed
author_role author
author2 Baldassini, Welder [UNESP]
Neto, Otávio Machado [UNESP]
Chardulo, Luis Artur [UNESP]
Torres, Rodrigo [UNESP]
Pereira, Guilherme [UNESP]
Curi, Rogério [UNESP]
Chiaratti, Marcos Roberto
Padilha, Pedro [UNESP]
Alessandroni, Laura
Gagaoua, Mohammed
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
Universidade Federal de São Carlos (UFSCar)
University of Camerino
Institut Agro
dc.contributor.author.fl_str_mv Santiago, Bismarck [UNESP]
Baldassini, Welder [UNESP]
Neto, Otávio Machado [UNESP]
Chardulo, Luis Artur [UNESP]
Torres, Rodrigo [UNESP]
Pereira, Guilherme [UNESP]
Curi, Rogério [UNESP]
Chiaratti, Marcos Roberto
Padilha, Pedro [UNESP]
Alessandroni, Laura
Gagaoua, Mohammed
dc.subject.por.fl_str_mv Beef tenderness
Feedlot, meat color, proteomics, biological mechanisms
Mass spectrometry
Meat quality
Proteome
topic Beef tenderness
Feedlot, meat color, proteomics, biological mechanisms
Mass spectrometry
Meat quality
Proteome
description This study investigated the skeletal muscle proteome of crossbred bulls and steers with the aim of explaining the differences in carcass and meat quality traits. Therefore, 640 post-weaning Angus-Nellore calves were fed a high-energy diet for a period of 180 days. In the feedlot trial, comparisons of steers (n = 320) and bulls (n = 320) showed lower (P < 0.01) average daily gain (1.38 vs. 1.60 ± 0.05 kg/d), final body weight (547.4 vs. 585.1 ± 9.3 kg), which resulted in lower hot carcass weight (298.4 vs. 333.7 ± 7.7 kg) and ribeye area (68.6 vs. 81.0 ± 2.56 cm2). Steers had higher (P < 0.01) carcass fatness, meat color parameters (L*, a*, b*, chroma (C*), hue (h°)) and lower ultimate pH. Moreover, lower (P < 0.01) Warner-Bratzler shear force (WBSF) were observed in steers compared to bulls (WBSF = 3.68 vs. 4.97 ± 0.08 kg; and 3.19 vs. 4.08 ± 0.08 kg). The proteomic approach using two-dimensional electrophoresis, mass spectrometry and bioinformatics procedures revealed several differentially expressed proteins between steers and bulls (P < 0.05). Interconnected pathways and substantial changes were revealed in biological processes, molecular functions, and cellular components between the post-mortem muscle proteomes of the compared animals. Steers had increased (P < 0.05) abundance of proteins related to energy metabolism (CKM, ALDOA, and GAPDH), and bulls had greater abundance of proteins associated with catabolic (glycolysis) processes (PGM1); oxidative stress (HSP60, HSPA8 and GSTP1); and muscle structure and contraction (TNNI2 and TNNT3). The better carcass (fatness and marbling degree) and meat quality traits (tenderness and color parameters) of steers were associated with higher abundance of key proteins of energy metabolism and lower abundance of enzymes related to catabolic processes, oxidative stress, and proteins of muscle contraction Significance: Sexual condition of cattle is known to be an important factor affecting animal performances and growth as well as the carcass and meat quality traits. The investigation of skeletal muscle proteome help a better understanding of the origin of the differences in quality traits between bulls and steers. The inferior meat quality of bulls was found to be due to the greater expression of proteins associated with primary and catabolic processes, oxidative stress, and muscle contraction. Steers had greater expression of proteins, from which several are known biomarkers of beef quality (mainly tenderness).
publishDate 2023
dc.date.none.fl_str_mv 2023-07-29T12:57:34Z
2023-07-29T12:57:34Z
2023-04-30
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.jprot.2023.104871
Journal of Proteomics, v. 278.
1876-7737
1874-3919
http://hdl.handle.net/11449/247046
10.1016/j.jprot.2023.104871
2-s2.0-85150882486
url http://dx.doi.org/10.1016/j.jprot.2023.104871
http://hdl.handle.net/11449/247046
identifier_str_mv Journal of Proteomics, v. 278.
1876-7737
1874-3919
10.1016/j.jprot.2023.104871
2-s2.0-85150882486
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of Proteomics
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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