Escherichia coli strains isolated from the uteri horn, mouth, and rectum of bitches suffering from pyometra: virulence factors, antimicrobial susceptibilities, and clonal relationships among strains
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1155/2014/979584 http://hdl.handle.net/11449/131024 |
Resumo: | Pyometra is recognized as one of the main causes of disease and death in the bitch, and Escherichia coli is the major pathogen associated with this disease. In this study, 70 E. coli isolates from the uteri horn, mouth, and rectum of bitches suffering from the disease and 43 E. coli isolates from the rectum of clinically healthy bitches were examined for the presence of uropathogenic virulence genes and susceptibility to antimicrobial drugs. DNA profiles of isolates from uteri horn and mouth in bitches with pyometra were compared by REP, ERIC, and BOX-PCR. Virulence gene frequencies detected in isolates from canine pyometra were as follows: 95.7% fim, 27.1% iss, 25.7% hly, 18.5% iuc, and 17.1% usp. Predominant resistance was determined for cephalothin, ampicillin, and nalidixic acid among the isolates from all sites examined. Multidrug resistance was found on ∼ 50% pyometra isolates. Using the genotypic methods some isolates from uteri, pus, and saliva of the same bitch proved to have identical DNA profiles which is a reason for concern due to the close relationship between household pets and humans. |
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Escherichia coli strains isolated from the uteri horn, mouth, and rectum of bitches suffering from pyometra: virulence factors, antimicrobial susceptibilities, and clonal relationships among strainsPyometra is recognized as one of the main causes of disease and death in the bitch, and Escherichia coli is the major pathogen associated with this disease. In this study, 70 E. coli isolates from the uteri horn, mouth, and rectum of bitches suffering from the disease and 43 E. coli isolates from the rectum of clinically healthy bitches were examined for the presence of uropathogenic virulence genes and susceptibility to antimicrobial drugs. DNA profiles of isolates from uteri horn and mouth in bitches with pyometra were compared by REP, ERIC, and BOX-PCR. Virulence gene frequencies detected in isolates from canine pyometra were as follows: 95.7% fim, 27.1% iss, 25.7% hly, 18.5% iuc, and 17.1% usp. Predominant resistance was determined for cephalothin, ampicillin, and nalidixic acid among the isolates from all sites examined. Multidrug resistance was found on ∼ 50% pyometra isolates. Using the genotypic methods some isolates from uteri, pus, and saliva of the same bitch proved to have identical DNA profiles which is a reason for concern due to the close relationship between household pets and humans.Programa de Microbiologia Agropecuária, Faculdade de Ciências Agrárias e Veterinárias, UNESP-Campus de Jaboticabal, 14884-900 Jaboticabal, SP, Brazil.Departamento de Medicina Veterinária Preventiva, Faculdade de Ciências Agrárias e Veterinárias, UNESP-Campus de Jaboticabal, 14884-900 Jaboticabal, SP, Brazil.Departamento de Patologia Veterinária, Faculdade de Ciências Agrárias e Veterinárias, UNESP-Campus de Jaboticabal, 14884-900 Jaboticabal, SP, Brazil.Departamento de Morfologia, Fisiologia e Patologia Básica, FORP, Avenida do Café S/N, Campus USP, 14040-904 Ribeirão Preto, SP, Brazil.Programa de Microbiologia Agropecuária, Faculdade de Ciências Agrárias e Veterinárias, UNESP-Campus de Jaboticabal, 14884-900 Jaboticabal, SP, Brazil.Departamento de Medicina Veterinária Preventiva, Faculdade de Ciências Agrárias e Veterinárias, UNESP-Campus de Jaboticabal, 14884-900 Jaboticabal, SP, Brazil.Departamento de Patologia Veterinária, Faculdade de Ciências Agrárias e Veterinárias, UNESP-Campus de Jaboticabal, 14884-900 Jaboticabal, SP, Brazil.International Journal Of MicrobiologyUniversidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Agostinho, Juliana M. A. [UNESP]Souza, Andressa de [UNESP]Schocken-Iturrino, Ruben P. [UNESP]Beraldo, Lívia G. [UNESP]Borges, Clarissa A. [UNESP]Avila, Fernando A. [UNESP]Marin, José M.2015-12-07T15:30:58Z2015-12-07T15:30:58Z2014info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1155/2014/979584International Journal Of Microbiology, v. 2014, 2014.1687-918Xhttp://hdl.handle.net/11449/13102410.1155/2014/979584PMC3966421.pdf24734047PMC3966421PubMedreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInternational Journal Of Microbiologyinfo:eu-repo/semantics/openAccess2024-06-07T13:01:28Zoai:repositorio.unesp.br:11449/131024Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-06-07T13:01:28Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Escherichia coli strains isolated from the uteri horn, mouth, and rectum of bitches suffering from pyometra: virulence factors, antimicrobial susceptibilities, and clonal relationships among strains |
title |
Escherichia coli strains isolated from the uteri horn, mouth, and rectum of bitches suffering from pyometra: virulence factors, antimicrobial susceptibilities, and clonal relationships among strains |
spellingShingle |
Escherichia coli strains isolated from the uteri horn, mouth, and rectum of bitches suffering from pyometra: virulence factors, antimicrobial susceptibilities, and clonal relationships among strains Agostinho, Juliana M. A. [UNESP] |
title_short |
Escherichia coli strains isolated from the uteri horn, mouth, and rectum of bitches suffering from pyometra: virulence factors, antimicrobial susceptibilities, and clonal relationships among strains |
title_full |
Escherichia coli strains isolated from the uteri horn, mouth, and rectum of bitches suffering from pyometra: virulence factors, antimicrobial susceptibilities, and clonal relationships among strains |
title_fullStr |
Escherichia coli strains isolated from the uteri horn, mouth, and rectum of bitches suffering from pyometra: virulence factors, antimicrobial susceptibilities, and clonal relationships among strains |
title_full_unstemmed |
Escherichia coli strains isolated from the uteri horn, mouth, and rectum of bitches suffering from pyometra: virulence factors, antimicrobial susceptibilities, and clonal relationships among strains |
title_sort |
Escherichia coli strains isolated from the uteri horn, mouth, and rectum of bitches suffering from pyometra: virulence factors, antimicrobial susceptibilities, and clonal relationships among strains |
author |
Agostinho, Juliana M. A. [UNESP] |
author_facet |
Agostinho, Juliana M. A. [UNESP] Souza, Andressa de [UNESP] Schocken-Iturrino, Ruben P. [UNESP] Beraldo, Lívia G. [UNESP] Borges, Clarissa A. [UNESP] Avila, Fernando A. [UNESP] Marin, José M. |
author_role |
author |
author2 |
Souza, Andressa de [UNESP] Schocken-Iturrino, Ruben P. [UNESP] Beraldo, Lívia G. [UNESP] Borges, Clarissa A. [UNESP] Avila, Fernando A. [UNESP] Marin, José M. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Agostinho, Juliana M. A. [UNESP] Souza, Andressa de [UNESP] Schocken-Iturrino, Ruben P. [UNESP] Beraldo, Lívia G. [UNESP] Borges, Clarissa A. [UNESP] Avila, Fernando A. [UNESP] Marin, José M. |
description |
Pyometra is recognized as one of the main causes of disease and death in the bitch, and Escherichia coli is the major pathogen associated with this disease. In this study, 70 E. coli isolates from the uteri horn, mouth, and rectum of bitches suffering from the disease and 43 E. coli isolates from the rectum of clinically healthy bitches were examined for the presence of uropathogenic virulence genes and susceptibility to antimicrobial drugs. DNA profiles of isolates from uteri horn and mouth in bitches with pyometra were compared by REP, ERIC, and BOX-PCR. Virulence gene frequencies detected in isolates from canine pyometra were as follows: 95.7% fim, 27.1% iss, 25.7% hly, 18.5% iuc, and 17.1% usp. Predominant resistance was determined for cephalothin, ampicillin, and nalidixic acid among the isolates from all sites examined. Multidrug resistance was found on ∼ 50% pyometra isolates. Using the genotypic methods some isolates from uteri, pus, and saliva of the same bitch proved to have identical DNA profiles which is a reason for concern due to the close relationship between household pets and humans. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014 2015-12-07T15:30:58Z 2015-12-07T15:30:58Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1155/2014/979584 International Journal Of Microbiology, v. 2014, 2014. 1687-918X http://hdl.handle.net/11449/131024 10.1155/2014/979584 PMC3966421.pdf 24734047 PMC3966421 |
url |
http://dx.doi.org/10.1155/2014/979584 http://hdl.handle.net/11449/131024 |
identifier_str_mv |
International Journal Of Microbiology, v. 2014, 2014. 1687-918X 10.1155/2014/979584 PMC3966421.pdf 24734047 PMC3966421 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
International Journal Of Microbiology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
International Journal Of Microbiology |
publisher.none.fl_str_mv |
International Journal Of Microbiology |
dc.source.none.fl_str_mv |
PubMed reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1803649363714506752 |