Detecting animal by-product intake using stable isotope ratio mass spectrometry (IRMS)
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.tvjl.2016.10.002 http://hdl.handle.net/11449/173731 |
Resumo: | Sheep are used in many countries as food and for manufacturing bioproducts. However, when these animals consume animal by-products (ABP), which is widely prohibited, there is a risk of transmitting scrapie – a fatal prion disease in human beings. Therefore, it is essential to develop sensitive methods to detect previous ABP intake to select safe animals for producing biopharmaceuticals. We used stable isotope ratio mass spectrometry (IRMS) for 13C and 15N to trace animal proteins in the serum of three groups of sheep: 1 – received only vegetable protein (VP) for 89 days; 2 – received animal and vegetable protein (AVP); and 3 – received animal and vegetable protein with animal protein subsequently removed (AVPR). Groups 2 and 3 received diets with 30% bovine meat and bone meal (MBM) added to a vegetable diet (from days 16–89 in the AVP group and until day 49 in the AVPR group, when MBM was removed). The AVPR group showed 15N equilibrium 5 days after MBM removal (54th day). Conversely, 15N equilibrium in the AVP group occurred 22 days later (76th day). The half-life differed between these groups by 3.55 days. In the AVPR group, 15N elimination required 53 days, which was similar to this isotope's incorporation time. Turnover was determined based on natural 15N signatures. IRMS followed by turnover calculations was used to evaluate the time period for the incorporation and elimination of animal protein in sheep serum. The δ13C and δ15N values were used to track animal protein in the diet. This method is biologically and economically relevant for the veterinary field because it can track protein over time or make a point assessment of animal feed with high sensitivity and resolution, providing a low-cost analysis coupled with fast detection. Isotopic profiles could be measured throughout the experimental period, demonstrating the potential to use the method for traceability and certification assessments. |
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Detecting animal by-product intake using stable isotope ratio mass spectrometry (IRMS)Animal by-product intakeSheepStable isotopesδ13C valueδ15N valueSheep are used in many countries as food and for manufacturing bioproducts. However, when these animals consume animal by-products (ABP), which is widely prohibited, there is a risk of transmitting scrapie – a fatal prion disease in human beings. Therefore, it is essential to develop sensitive methods to detect previous ABP intake to select safe animals for producing biopharmaceuticals. We used stable isotope ratio mass spectrometry (IRMS) for 13C and 15N to trace animal proteins in the serum of three groups of sheep: 1 – received only vegetable protein (VP) for 89 days; 2 – received animal and vegetable protein (AVP); and 3 – received animal and vegetable protein with animal protein subsequently removed (AVPR). Groups 2 and 3 received diets with 30% bovine meat and bone meal (MBM) added to a vegetable diet (from days 16–89 in the AVP group and until day 49 in the AVPR group, when MBM was removed). The AVPR group showed 15N equilibrium 5 days after MBM removal (54th day). Conversely, 15N equilibrium in the AVP group occurred 22 days later (76th day). The half-life differed between these groups by 3.55 days. In the AVPR group, 15N elimination required 53 days, which was similar to this isotope's incorporation time. Turnover was determined based on natural 15N signatures. IRMS followed by turnover calculations was used to evaluate the time period for the incorporation and elimination of animal protein in sheep serum. The δ13C and δ15N values were used to track animal protein in the diet. This method is biologically and economically relevant for the veterinary field because it can track protein over time or make a point assessment of animal feed with high sensitivity and resolution, providing a low-cost analysis coupled with fast detection. Isotopic profiles could be measured throughout the experimental period, demonstrating the potential to use the method for traceability and certification assessments.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Center for the Study of Venoms and Venomous Animals (CEVAP) UNESP – Univ Estadual PaulistaBotucatu Medical School UNESP – Univ Estadual PaulistaCollege of Agricultural Sciences UNESP – Univ Estadual PaulistaStable Isotopes Center (CIE) UNESP – Univ Estadual PaulistaCollege of Veterinary Medicine and Animal Husbandry (FMVZ) UNESP – Univ Estadual PaulistaCenter for the Study of Venoms and Venomous Animals (CEVAP) UNESP – Univ Estadual PaulistaBotucatu Medical School UNESP – Univ Estadual PaulistaCollege of Agricultural Sciences UNESP – Univ Estadual PaulistaStable Isotopes Center (CIE) UNESP – Univ Estadual PaulistaCollege of Veterinary Medicine and Animal Husbandry (FMVZ) UNESP – Univ Estadual PaulistaCAPES: 23038.006285/2011-21CAPES: 23038.008557/2010CNPq: 563582/2010-3Universidade Estadual Paulista (Unesp)da Silva, D. A.F. [UNESP]Biscola, N. P. [UNESP]dos Santos, L. D. [UNESP]Sartori, M. M.P. [UNESP]Denadai, J. C. [UNESP]da Silva, E. T. [UNESP]Ducatti, C. [UNESP]Bicudo, S. D. [UNESP]Barraviera, B. [UNESP]Ferreira, R. S. [UNESP]2018-12-11T17:07:29Z2018-12-11T17:07:29Z2016-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article119-125application/pdfhttp://dx.doi.org/10.1016/j.tvjl.2016.10.002Veterinary Journal, v. 217, p. 119-125.1532-29711090-0233http://hdl.handle.net/11449/17373110.1016/j.tvjl.2016.10.0022-s2.0-849943861222-s2.0-84994386122.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengVeterinary Journal0,979info:eu-repo/semantics/openAccess2024-04-11T15:28:26Zoai:repositorio.unesp.br:11449/173731Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:52:10.518057Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Detecting animal by-product intake using stable isotope ratio mass spectrometry (IRMS) |
title |
Detecting animal by-product intake using stable isotope ratio mass spectrometry (IRMS) |
spellingShingle |
Detecting animal by-product intake using stable isotope ratio mass spectrometry (IRMS) da Silva, D. A.F. [UNESP] Animal by-product intake Sheep Stable isotopes δ13C value δ15N value |
title_short |
Detecting animal by-product intake using stable isotope ratio mass spectrometry (IRMS) |
title_full |
Detecting animal by-product intake using stable isotope ratio mass spectrometry (IRMS) |
title_fullStr |
Detecting animal by-product intake using stable isotope ratio mass spectrometry (IRMS) |
title_full_unstemmed |
Detecting animal by-product intake using stable isotope ratio mass spectrometry (IRMS) |
title_sort |
Detecting animal by-product intake using stable isotope ratio mass spectrometry (IRMS) |
author |
da Silva, D. A.F. [UNESP] |
author_facet |
da Silva, D. A.F. [UNESP] Biscola, N. P. [UNESP] dos Santos, L. D. [UNESP] Sartori, M. M.P. [UNESP] Denadai, J. C. [UNESP] da Silva, E. T. [UNESP] Ducatti, C. [UNESP] Bicudo, S. D. [UNESP] Barraviera, B. [UNESP] Ferreira, R. S. [UNESP] |
author_role |
author |
author2 |
Biscola, N. P. [UNESP] dos Santos, L. D. [UNESP] Sartori, M. M.P. [UNESP] Denadai, J. C. [UNESP] da Silva, E. T. [UNESP] Ducatti, C. [UNESP] Bicudo, S. D. [UNESP] Barraviera, B. [UNESP] Ferreira, R. S. [UNESP] |
author2_role |
author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
da Silva, D. A.F. [UNESP] Biscola, N. P. [UNESP] dos Santos, L. D. [UNESP] Sartori, M. M.P. [UNESP] Denadai, J. C. [UNESP] da Silva, E. T. [UNESP] Ducatti, C. [UNESP] Bicudo, S. D. [UNESP] Barraviera, B. [UNESP] Ferreira, R. S. [UNESP] |
dc.subject.por.fl_str_mv |
Animal by-product intake Sheep Stable isotopes δ13C value δ15N value |
topic |
Animal by-product intake Sheep Stable isotopes δ13C value δ15N value |
description |
Sheep are used in many countries as food and for manufacturing bioproducts. However, when these animals consume animal by-products (ABP), which is widely prohibited, there is a risk of transmitting scrapie – a fatal prion disease in human beings. Therefore, it is essential to develop sensitive methods to detect previous ABP intake to select safe animals for producing biopharmaceuticals. We used stable isotope ratio mass spectrometry (IRMS) for 13C and 15N to trace animal proteins in the serum of three groups of sheep: 1 – received only vegetable protein (VP) for 89 days; 2 – received animal and vegetable protein (AVP); and 3 – received animal and vegetable protein with animal protein subsequently removed (AVPR). Groups 2 and 3 received diets with 30% bovine meat and bone meal (MBM) added to a vegetable diet (from days 16–89 in the AVP group and until day 49 in the AVPR group, when MBM was removed). The AVPR group showed 15N equilibrium 5 days after MBM removal (54th day). Conversely, 15N equilibrium in the AVP group occurred 22 days later (76th day). The half-life differed between these groups by 3.55 days. In the AVPR group, 15N elimination required 53 days, which was similar to this isotope's incorporation time. Turnover was determined based on natural 15N signatures. IRMS followed by turnover calculations was used to evaluate the time period for the incorporation and elimination of animal protein in sheep serum. The δ13C and δ15N values were used to track animal protein in the diet. This method is biologically and economically relevant for the veterinary field because it can track protein over time or make a point assessment of animal feed with high sensitivity and resolution, providing a low-cost analysis coupled with fast detection. Isotopic profiles could be measured throughout the experimental period, demonstrating the potential to use the method for traceability and certification assessments. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-11-01 2018-12-11T17:07:29Z 2018-12-11T17:07:29Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.tvjl.2016.10.002 Veterinary Journal, v. 217, p. 119-125. 1532-2971 1090-0233 http://hdl.handle.net/11449/173731 10.1016/j.tvjl.2016.10.002 2-s2.0-84994386122 2-s2.0-84994386122.pdf |
url |
http://dx.doi.org/10.1016/j.tvjl.2016.10.002 http://hdl.handle.net/11449/173731 |
identifier_str_mv |
Veterinary Journal, v. 217, p. 119-125. 1532-2971 1090-0233 10.1016/j.tvjl.2016.10.002 2-s2.0-84994386122 2-s2.0-84994386122.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Veterinary Journal 0,979 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
119-125 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129132135972864 |