Detecting animal by-product intake using stable isotope ratio mass spectrometry (IRMS)

Detalhes bibliográficos
Autor(a) principal: da Silva, D. A.F. [UNESP]
Data de Publicação: 2016
Outros Autores: Biscola, N. P. [UNESP], dos Santos, L. D. [UNESP], Sartori, M. M.P. [UNESP], Denadai, J. C. [UNESP], da Silva, E. T. [UNESP], Ducatti, C. [UNESP], Bicudo, S. D. [UNESP], Barraviera, B. [UNESP], Ferreira, R. S. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.tvjl.2016.10.002
http://hdl.handle.net/11449/173731
Resumo: Sheep are used in many countries as food and for manufacturing bioproducts. However, when these animals consume animal by-products (ABP), which is widely prohibited, there is a risk of transmitting scrapie – a fatal prion disease in human beings. Therefore, it is essential to develop sensitive methods to detect previous ABP intake to select safe animals for producing biopharmaceuticals. We used stable isotope ratio mass spectrometry (IRMS) for 13C and 15N to trace animal proteins in the serum of three groups of sheep: 1 – received only vegetable protein (VP) for 89 days; 2 – received animal and vegetable protein (AVP); and 3 – received animal and vegetable protein with animal protein subsequently removed (AVPR). Groups 2 and 3 received diets with 30% bovine meat and bone meal (MBM) added to a vegetable diet (from days 16–89 in the AVP group and until day 49 in the AVPR group, when MBM was removed). The AVPR group showed 15N equilibrium 5 days after MBM removal (54th day). Conversely, 15N equilibrium in the AVP group occurred 22 days later (76th day). The half-life differed between these groups by 3.55 days. In the AVPR group, 15N elimination required 53 days, which was similar to this isotope's incorporation time. Turnover was determined based on natural 15N signatures. IRMS followed by turnover calculations was used to evaluate the time period for the incorporation and elimination of animal protein in sheep serum. The δ13C and δ15N values were used to track animal protein in the diet. This method is biologically and economically relevant for the veterinary field because it can track protein over time or make a point assessment of animal feed with high sensitivity and resolution, providing a low-cost analysis coupled with fast detection. Isotopic profiles could be measured throughout the experimental period, demonstrating the potential to use the method for traceability and certification assessments.
id UNSP_cbe5d995a6979593e100697395713de4
oai_identifier_str oai:repositorio.unesp.br:11449/173731
network_acronym_str UNSP
network_name_str Repositório Institucional da UNESP
repository_id_str 2946
spelling Detecting animal by-product intake using stable isotope ratio mass spectrometry (IRMS)Animal by-product intakeSheepStable isotopesδ13C valueδ15N valueSheep are used in many countries as food and for manufacturing bioproducts. However, when these animals consume animal by-products (ABP), which is widely prohibited, there is a risk of transmitting scrapie – a fatal prion disease in human beings. Therefore, it is essential to develop sensitive methods to detect previous ABP intake to select safe animals for producing biopharmaceuticals. We used stable isotope ratio mass spectrometry (IRMS) for 13C and 15N to trace animal proteins in the serum of three groups of sheep: 1 – received only vegetable protein (VP) for 89 days; 2 – received animal and vegetable protein (AVP); and 3 – received animal and vegetable protein with animal protein subsequently removed (AVPR). Groups 2 and 3 received diets with 30% bovine meat and bone meal (MBM) added to a vegetable diet (from days 16–89 in the AVP group and until day 49 in the AVPR group, when MBM was removed). The AVPR group showed 15N equilibrium 5 days after MBM removal (54th day). Conversely, 15N equilibrium in the AVP group occurred 22 days later (76th day). The half-life differed between these groups by 3.55 days. In the AVPR group, 15N elimination required 53 days, which was similar to this isotope's incorporation time. Turnover was determined based on natural 15N signatures. IRMS followed by turnover calculations was used to evaluate the time period for the incorporation and elimination of animal protein in sheep serum. The δ13C and δ15N values were used to track animal protein in the diet. This method is biologically and economically relevant for the veterinary field because it can track protein over time or make a point assessment of animal feed with high sensitivity and resolution, providing a low-cost analysis coupled with fast detection. Isotopic profiles could be measured throughout the experimental period, demonstrating the potential to use the method for traceability and certification assessments.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Center for the Study of Venoms and Venomous Animals (CEVAP) UNESP – Univ Estadual PaulistaBotucatu Medical School UNESP – Univ Estadual PaulistaCollege of Agricultural Sciences UNESP – Univ Estadual PaulistaStable Isotopes Center (CIE) UNESP – Univ Estadual PaulistaCollege of Veterinary Medicine and Animal Husbandry (FMVZ) UNESP – Univ Estadual PaulistaCenter for the Study of Venoms and Venomous Animals (CEVAP) UNESP – Univ Estadual PaulistaBotucatu Medical School UNESP – Univ Estadual PaulistaCollege of Agricultural Sciences UNESP – Univ Estadual PaulistaStable Isotopes Center (CIE) UNESP – Univ Estadual PaulistaCollege of Veterinary Medicine and Animal Husbandry (FMVZ) UNESP – Univ Estadual PaulistaCAPES: 23038.006285/2011-21CAPES: 23038.008557/2010CNPq: 563582/2010-3Universidade Estadual Paulista (Unesp)da Silva, D. A.F. [UNESP]Biscola, N. P. [UNESP]dos Santos, L. D. [UNESP]Sartori, M. M.P. [UNESP]Denadai, J. C. [UNESP]da Silva, E. T. [UNESP]Ducatti, C. [UNESP]Bicudo, S. D. [UNESP]Barraviera, B. [UNESP]Ferreira, R. S. [UNESP]2018-12-11T17:07:29Z2018-12-11T17:07:29Z2016-11-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article119-125application/pdfhttp://dx.doi.org/10.1016/j.tvjl.2016.10.002Veterinary Journal, v. 217, p. 119-125.1532-29711090-0233http://hdl.handle.net/11449/17373110.1016/j.tvjl.2016.10.0022-s2.0-849943861222-s2.0-84994386122.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengVeterinary Journal0,979info:eu-repo/semantics/openAccess2024-04-11T15:28:26Zoai:repositorio.unesp.br:11449/173731Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:52:10.518057Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Detecting animal by-product intake using stable isotope ratio mass spectrometry (IRMS)
title Detecting animal by-product intake using stable isotope ratio mass spectrometry (IRMS)
spellingShingle Detecting animal by-product intake using stable isotope ratio mass spectrometry (IRMS)
da Silva, D. A.F. [UNESP]
Animal by-product intake
Sheep
Stable isotopes
δ13C value
δ15N value
title_short Detecting animal by-product intake using stable isotope ratio mass spectrometry (IRMS)
title_full Detecting animal by-product intake using stable isotope ratio mass spectrometry (IRMS)
title_fullStr Detecting animal by-product intake using stable isotope ratio mass spectrometry (IRMS)
title_full_unstemmed Detecting animal by-product intake using stable isotope ratio mass spectrometry (IRMS)
title_sort Detecting animal by-product intake using stable isotope ratio mass spectrometry (IRMS)
author da Silva, D. A.F. [UNESP]
author_facet da Silva, D. A.F. [UNESP]
Biscola, N. P. [UNESP]
dos Santos, L. D. [UNESP]
Sartori, M. M.P. [UNESP]
Denadai, J. C. [UNESP]
da Silva, E. T. [UNESP]
Ducatti, C. [UNESP]
Bicudo, S. D. [UNESP]
Barraviera, B. [UNESP]
Ferreira, R. S. [UNESP]
author_role author
author2 Biscola, N. P. [UNESP]
dos Santos, L. D. [UNESP]
Sartori, M. M.P. [UNESP]
Denadai, J. C. [UNESP]
da Silva, E. T. [UNESP]
Ducatti, C. [UNESP]
Bicudo, S. D. [UNESP]
Barraviera, B. [UNESP]
Ferreira, R. S. [UNESP]
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv da Silva, D. A.F. [UNESP]
Biscola, N. P. [UNESP]
dos Santos, L. D. [UNESP]
Sartori, M. M.P. [UNESP]
Denadai, J. C. [UNESP]
da Silva, E. T. [UNESP]
Ducatti, C. [UNESP]
Bicudo, S. D. [UNESP]
Barraviera, B. [UNESP]
Ferreira, R. S. [UNESP]
dc.subject.por.fl_str_mv Animal by-product intake
Sheep
Stable isotopes
δ13C value
δ15N value
topic Animal by-product intake
Sheep
Stable isotopes
δ13C value
δ15N value
description Sheep are used in many countries as food and for manufacturing bioproducts. However, when these animals consume animal by-products (ABP), which is widely prohibited, there is a risk of transmitting scrapie – a fatal prion disease in human beings. Therefore, it is essential to develop sensitive methods to detect previous ABP intake to select safe animals for producing biopharmaceuticals. We used stable isotope ratio mass spectrometry (IRMS) for 13C and 15N to trace animal proteins in the serum of three groups of sheep: 1 – received only vegetable protein (VP) for 89 days; 2 – received animal and vegetable protein (AVP); and 3 – received animal and vegetable protein with animal protein subsequently removed (AVPR). Groups 2 and 3 received diets with 30% bovine meat and bone meal (MBM) added to a vegetable diet (from days 16–89 in the AVP group and until day 49 in the AVPR group, when MBM was removed). The AVPR group showed 15N equilibrium 5 days after MBM removal (54th day). Conversely, 15N equilibrium in the AVP group occurred 22 days later (76th day). The half-life differed between these groups by 3.55 days. In the AVPR group, 15N elimination required 53 days, which was similar to this isotope's incorporation time. Turnover was determined based on natural 15N signatures. IRMS followed by turnover calculations was used to evaluate the time period for the incorporation and elimination of animal protein in sheep serum. The δ13C and δ15N values were used to track animal protein in the diet. This method is biologically and economically relevant for the veterinary field because it can track protein over time or make a point assessment of animal feed with high sensitivity and resolution, providing a low-cost analysis coupled with fast detection. Isotopic profiles could be measured throughout the experimental period, demonstrating the potential to use the method for traceability and certification assessments.
publishDate 2016
dc.date.none.fl_str_mv 2016-11-01
2018-12-11T17:07:29Z
2018-12-11T17:07:29Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.tvjl.2016.10.002
Veterinary Journal, v. 217, p. 119-125.
1532-2971
1090-0233
http://hdl.handle.net/11449/173731
10.1016/j.tvjl.2016.10.002
2-s2.0-84994386122
2-s2.0-84994386122.pdf
url http://dx.doi.org/10.1016/j.tvjl.2016.10.002
http://hdl.handle.net/11449/173731
identifier_str_mv Veterinary Journal, v. 217, p. 119-125.
1532-2971
1090-0233
10.1016/j.tvjl.2016.10.002
2-s2.0-84994386122
2-s2.0-84994386122.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Veterinary Journal
0,979
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 119-125
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808129132135972864