Evaluation of critical parameters for in vitro skin permeation and penetration studies using animal skin models
Autor(a) principal: | |
---|---|
Data de Publicação: | 2018 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.ejps.2017.09.034 http://hdl.handle.net/11449/175276 |
Resumo: | In vitro skin permeation/penetration studies may be affected by many sources of variation. Herein, we aimed to investigate the major critical procedures of in vitro skin delivery studies. These experiments were performed with model drugs according to official guidelines. The influence of skin source on penetration studies was studied as well as the use of a cryopreservation agent on skin freezing evaluated by transepidermal water loss, electrical resistance, permeation/penetration profiles and histological changes of the skin. The best condition for tape stripping procedure was validated through the evaluation of the distribution of corneocytes, mass of stratum corneum (SC) removed and amount of protein removed using finger pressure, a 2 kg weight and a roller. The interchangeability of the tape stripping procedures followed by the epidermis and dermis homogenate and the micrometric horizontal cryostat skin sectioning methods were also investigated, besides the effect of different formulations. Noteworthy, different skin sources were able to ensure reliable interchangeability for in vitro permeation studies. Furthermore, an increased penetration was obtained for stored frozen skin compared to fresh skin, even with the addition of a cryoprotectant agent. The best method for tape stripping was the finger pressure followed by the addition of a propylene glycol solvent leading to better SC removal. Finally, no significant difference was found in skin penetration studies performed by different methods suggesting their possible interchangeability. |
id |
UNSP_ccdfd75ede3517852f2f0eb9870328ac |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/175276 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Evaluation of critical parameters for in vitro skin permeation and penetration studies using animal skin modelsCryopreservation agentFrozen/fresh skinIn vitro skin permeation/penetration studyMicrometric horizontal cryostat skin sectioningSkin sourceTape strippingIn vitro skin permeation/penetration studies may be affected by many sources of variation. Herein, we aimed to investigate the major critical procedures of in vitro skin delivery studies. These experiments were performed with model drugs according to official guidelines. The influence of skin source on penetration studies was studied as well as the use of a cryopreservation agent on skin freezing evaluated by transepidermal water loss, electrical resistance, permeation/penetration profiles and histological changes of the skin. The best condition for tape stripping procedure was validated through the evaluation of the distribution of corneocytes, mass of stratum corneum (SC) removed and amount of protein removed using finger pressure, a 2 kg weight and a roller. The interchangeability of the tape stripping procedures followed by the epidermis and dermis homogenate and the micrometric horizontal cryostat skin sectioning methods were also investigated, besides the effect of different formulations. Noteworthy, different skin sources were able to ensure reliable interchangeability for in vitro permeation studies. Furthermore, an increased penetration was obtained for stored frozen skin compared to fresh skin, even with the addition of a cryoprotectant agent. The best method for tape stripping was the finger pressure followed by the addition of a propylene glycol solvent leading to better SC removal. Finally, no significant difference was found in skin penetration studies performed by different methods suggesting their possible interchangeability.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Ministry of Science and TechnologySchool of Pharmaceutical Sciences of Ribeirão Preto University of São Paulo, Avenida do Café, s/nFaculdades Integradas Padre Albino – FIPAResearch Institute for Medicines (iMed.ULisboa) Faculdade de Farmácia University of LisbonSchool of Pharmaceutical Sciences UNESP – São Paulo State University Campus Araraquara Department of Drugs and Medicines, Rodovia Araraquara-Jau, km. 1School of Pharmaceutical Sciences UNESP – São Paulo State University Campus Araraquara Department of Drugs and Medicines, Rodovia Araraquara-Jau, km. 1FAPESP: 04/09465-7FAPESP: 2013/16617-1Universidade de São Paulo (USP)Faculdades Integradas Padre Albino – FIPAUniversity of LisbonUniversidade Estadual Paulista (Unesp)Praça, Fabíola Silva GarciaMedina, Wanessa Silva GarciaEloy, Josimar O. [UNESP]Petrilli, RaquelCampos, Patrícia MazurekiAscenso, AndreiaBentley, Maria Vitória L.B.2018-12-11T17:15:06Z2018-12-11T17:15:06Z2018-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article121-132application/pdfhttp://dx.doi.org/10.1016/j.ejps.2017.09.034European Journal of Pharmaceutical Sciences, v. 111, p. 121-132.1879-07200928-0987http://hdl.handle.net/11449/17527610.1016/j.ejps.2017.09.0342-s2.0-850303079842-s2.0-85030307984.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengEuropean Journal of Pharmaceutical Sciences1,016info:eu-repo/semantics/openAccess2024-06-24T13:46:11Zoai:repositorio.unesp.br:11449/175276Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:46:06.754405Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Evaluation of critical parameters for in vitro skin permeation and penetration studies using animal skin models |
title |
Evaluation of critical parameters for in vitro skin permeation and penetration studies using animal skin models |
spellingShingle |
Evaluation of critical parameters for in vitro skin permeation and penetration studies using animal skin models Praça, Fabíola Silva Garcia Cryopreservation agent Frozen/fresh skin In vitro skin permeation/penetration study Micrometric horizontal cryostat skin sectioning Skin source Tape stripping |
title_short |
Evaluation of critical parameters for in vitro skin permeation and penetration studies using animal skin models |
title_full |
Evaluation of critical parameters for in vitro skin permeation and penetration studies using animal skin models |
title_fullStr |
Evaluation of critical parameters for in vitro skin permeation and penetration studies using animal skin models |
title_full_unstemmed |
Evaluation of critical parameters for in vitro skin permeation and penetration studies using animal skin models |
title_sort |
Evaluation of critical parameters for in vitro skin permeation and penetration studies using animal skin models |
author |
Praça, Fabíola Silva Garcia |
author_facet |
Praça, Fabíola Silva Garcia Medina, Wanessa Silva Garcia Eloy, Josimar O. [UNESP] Petrilli, Raquel Campos, Patrícia Mazureki Ascenso, Andreia Bentley, Maria Vitória L.B. |
author_role |
author |
author2 |
Medina, Wanessa Silva Garcia Eloy, Josimar O. [UNESP] Petrilli, Raquel Campos, Patrícia Mazureki Ascenso, Andreia Bentley, Maria Vitória L.B. |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade de São Paulo (USP) Faculdades Integradas Padre Albino – FIPA University of Lisbon Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Praça, Fabíola Silva Garcia Medina, Wanessa Silva Garcia Eloy, Josimar O. [UNESP] Petrilli, Raquel Campos, Patrícia Mazureki Ascenso, Andreia Bentley, Maria Vitória L.B. |
dc.subject.por.fl_str_mv |
Cryopreservation agent Frozen/fresh skin In vitro skin permeation/penetration study Micrometric horizontal cryostat skin sectioning Skin source Tape stripping |
topic |
Cryopreservation agent Frozen/fresh skin In vitro skin permeation/penetration study Micrometric horizontal cryostat skin sectioning Skin source Tape stripping |
description |
In vitro skin permeation/penetration studies may be affected by many sources of variation. Herein, we aimed to investigate the major critical procedures of in vitro skin delivery studies. These experiments were performed with model drugs according to official guidelines. The influence of skin source on penetration studies was studied as well as the use of a cryopreservation agent on skin freezing evaluated by transepidermal water loss, electrical resistance, permeation/penetration profiles and histological changes of the skin. The best condition for tape stripping procedure was validated through the evaluation of the distribution of corneocytes, mass of stratum corneum (SC) removed and amount of protein removed using finger pressure, a 2 kg weight and a roller. The interchangeability of the tape stripping procedures followed by the epidermis and dermis homogenate and the micrometric horizontal cryostat skin sectioning methods were also investigated, besides the effect of different formulations. Noteworthy, different skin sources were able to ensure reliable interchangeability for in vitro permeation studies. Furthermore, an increased penetration was obtained for stored frozen skin compared to fresh skin, even with the addition of a cryoprotectant agent. The best method for tape stripping was the finger pressure followed by the addition of a propylene glycol solvent leading to better SC removal. Finally, no significant difference was found in skin penetration studies performed by different methods suggesting their possible interchangeability. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-11T17:15:06Z 2018-12-11T17:15:06Z 2018-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.ejps.2017.09.034 European Journal of Pharmaceutical Sciences, v. 111, p. 121-132. 1879-0720 0928-0987 http://hdl.handle.net/11449/175276 10.1016/j.ejps.2017.09.034 2-s2.0-85030307984 2-s2.0-85030307984.pdf |
url |
http://dx.doi.org/10.1016/j.ejps.2017.09.034 http://hdl.handle.net/11449/175276 |
identifier_str_mv |
European Journal of Pharmaceutical Sciences, v. 111, p. 121-132. 1879-0720 0928-0987 10.1016/j.ejps.2017.09.034 2-s2.0-85030307984 2-s2.0-85030307984.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
European Journal of Pharmaceutical Sciences 1,016 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
121-132 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129246024957952 |